
ViRUSES AND PRiONS
... coat and other required proteins, including some enzymes needed for replication. Everything else is supplied by the host cell. Outside of a host cell, viruses cannot replicate and they show few if any of the properties commonly attributed to living things. However, once inside an appropriate cell, v ...
... coat and other required proteins, including some enzymes needed for replication. Everything else is supplied by the host cell. Outside of a host cell, viruses cannot replicate and they show few if any of the properties commonly attributed to living things. However, once inside an appropriate cell, v ...
1495/Chapter 07
... quantity of DNA in somatic cells is both constant and double the quantity of DNA in gametes. Since at each mating two gametes come together to produce a zygote with a full complement of hereditary material, you would expect reproductive cells to have only half as much hereditary material as the cell ...
... quantity of DNA in somatic cells is both constant and double the quantity of DNA in gametes. Since at each mating two gametes come together to produce a zygote with a full complement of hereditary material, you would expect reproductive cells to have only half as much hereditary material as the cell ...
Lec 13 Microbial diseases of skin and eyes
... Candidiasis may result from suppression of competing bacteria by antibiotics Occurs in skin; mucous membranes of genitourinary tract and mouth Thrush is an infection of mucous membranes of mouth Topical treatment with miconazole or ...
... Candidiasis may result from suppression of competing bacteria by antibiotics Occurs in skin; mucous membranes of genitourinary tract and mouth Thrush is an infection of mucous membranes of mouth Topical treatment with miconazole or ...
Microbiology 1: Bacterial Properties
... They grow with a very rapid generation time: e.g. Streptococcus pyogenes ...
... They grow with a very rapid generation time: e.g. Streptococcus pyogenes ...
EVD - UNSSECAA
... with EVO patients should be avoided. o It is important to always perform hand hygiene with soap and running water when hands are visibly soiled. o •Alcohol-based hand rubs should be made available at every point of care (at the entrance and within the Isolation rooms and areas); o Running water, soa ...
... with EVO patients should be avoided. o It is important to always perform hand hygiene with soap and running water when hands are visibly soiled. o •Alcohol-based hand rubs should be made available at every point of care (at the entrance and within the Isolation rooms and areas); o Running water, soa ...
bacteria and fungi Reproduction
... Disinfectants kill / inhibit growth of harmful / pathogenic microorganisms - reduce chance of ...
... Disinfectants kill / inhibit growth of harmful / pathogenic microorganisms - reduce chance of ...
(Macroparasites and microparasites)edited [Recovered]
... Viruses • Viruses are infectious agents that can only replicate inside a host cell. Viruses contain genetic material in the form of DNA or RNA, packaged in a protective protein coat. • Some viruses have a lipid membrane surround derived from host cell materials. The outer surface of a virus contain ...
... Viruses • Viruses are infectious agents that can only replicate inside a host cell. Viruses contain genetic material in the form of DNA or RNA, packaged in a protective protein coat. • Some viruses have a lipid membrane surround derived from host cell materials. The outer surface of a virus contain ...
International Society for Analytical Cytology Biosafety Standard for
... bronchoalveolar lavage, cerebrospinal fluid, blood Blood ...
... bronchoalveolar lavage, cerebrospinal fluid, blood Blood ...
ROLE DU LABORATOIRE
... the sequence of the amplicon need not be known in order to detect it. The technology offers advantages over routine single-target and multiplex PCR in that it is a full bioinformatics sequence analysis system. ...
... the sequence of the amplicon need not be known in order to detect it. The technology offers advantages over routine single-target and multiplex PCR in that it is a full bioinformatics sequence analysis system. ...
Exploring the Normal Flora of the Human Body
... Because of the complex chemical composition of the slime, biofilms are resistant to immune systems, disinfectants, and antibiotics (about 1000 times more resistant). Significant problem on internal medical devices such as catheters, stents, and mechanical heart valves; also problematic on contac ...
... Because of the complex chemical composition of the slime, biofilms are resistant to immune systems, disinfectants, and antibiotics (about 1000 times more resistant). Significant problem on internal medical devices such as catheters, stents, and mechanical heart valves; also problematic on contac ...
Hepatitis A-E Viruses part ІІ
... – Acute symptoms include jaundice, nausea, abdominal pain, loss of appetite, dark urine ...
... – Acute symptoms include jaundice, nausea, abdominal pain, loss of appetite, dark urine ...
AntibioticsVirusesED
... http://en.wikipedia.org/wiki/Image:Staphylococcus_aureus_%28AB_Test%29.jpg Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College. ...
... http://en.wikipedia.org/wiki/Image:Staphylococcus_aureus_%28AB_Test%29.jpg Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College. ...
STAC RESTRUCTURE
... Production of Novel Ebola Virus-Like Particles from cDNAs: an Alternative to Ebola Virus Generation by Reverse Genetics ...
... Production of Novel Ebola Virus-Like Particles from cDNAs: an Alternative to Ebola Virus Generation by Reverse Genetics ...
Microbial Mechanisms of Pathogenicity (Chapter 15) Lecture
... -Neisseria then grows inside these cells C. Mycolic acid (waxy) of Mycobacterium ...
... -Neisseria then grows inside these cells C. Mycolic acid (waxy) of Mycobacterium ...
Influence of insertion site of the avian influenza virus haemagglutinin
... CEFs, separated in denaturing agarose gels, transferred to nylon membranes and hybridized with 32P-labelled antisense RNA specific for AIV H5, AIV N1 or NDV P. The signals were quantified by radioluminography using a FLA3000 scanner (Fujifilm) and Advanced Image Data Analyser software (Raytest) for ...
... CEFs, separated in denaturing agarose gels, transferred to nylon membranes and hybridized with 32P-labelled antisense RNA specific for AIV H5, AIV N1 or NDV P. The signals were quantified by radioluminography using a FLA3000 scanner (Fujifilm) and Advanced Image Data Analyser software (Raytest) for ...
Viruses and Evolution Student Recording Sheet
... Controlling the evolution of HIV may be the way to control this constantly adapting virus. ...
... Controlling the evolution of HIV may be the way to control this constantly adapting virus. ...
Chapter 18: Infectious Diseases Affecting the Skin and Eyes
... chickenpox except they may last for weeks B) Inflammation of portions of the neurons may also lead to pain and tenderness that can last for months 4. Because the chickenpox lesions are itchy, many children are prone to secondary infection by S. pyogenes or S. aureus ...
... chickenpox except they may last for weeks B) Inflammation of portions of the neurons may also lead to pain and tenderness that can last for months 4. Because the chickenpox lesions are itchy, many children are prone to secondary infection by S. pyogenes or S. aureus ...
The Genetics of Bacteria and Viruses
... a retrovirus After HIV enters the host cell, reverse transcriptase synthesizes double stranded DNA from the viral RNA Transcription produces more copies of the viral RNA that are translated into viral proteins, which self-assemble into a virus particle and leave the host ...
... a retrovirus After HIV enters the host cell, reverse transcriptase synthesizes double stranded DNA from the viral RNA Transcription produces more copies of the viral RNA that are translated into viral proteins, which self-assemble into a virus particle and leave the host ...
Detection and Identification of Microorganisms
... • The most widely used methods for HIV detection are the ELISA assays, the enzyme-linked immunosorbant assays, which can come in the form of enzyme immunoassay or chemoluminescent immunoassay. They can detect either HIV-1 antibodies, or HIV-2 antibodies, or HIV-1 p24 antigen, or a combination of HIV ...
... • The most widely used methods for HIV detection are the ELISA assays, the enzyme-linked immunosorbant assays, which can come in the form of enzyme immunoassay or chemoluminescent immunoassay. They can detect either HIV-1 antibodies, or HIV-2 antibodies, or HIV-1 p24 antigen, or a combination of HIV ...
Attack of the Viruses!
... detailing the specific points they learned from the lesson. Resources/Materials needed: 11 X 17 paper for each student, markers, crayons, colored pencils, overhead projector and computer with powerpoint and media player, digital file of Discovery School’s Biology Concepts – Viruses, Styrofoam cups f ...
... detailing the specific points they learned from the lesson. Resources/Materials needed: 11 X 17 paper for each student, markers, crayons, colored pencils, overhead projector and computer with powerpoint and media player, digital file of Discovery School’s Biology Concepts – Viruses, Styrofoam cups f ...
Microscopy - WordPress.com
... (deoxyribonucleic acid), RNA (ribonucleic acid), or proteins of an infectious agent in a clinical sample can be used to help identify the agent. In many cases the agent can be detected and identified in this way, even if it cannot be isolated or detected by immunologic means. New techniques and ad ...
... (deoxyribonucleic acid), RNA (ribonucleic acid), or proteins of an infectious agent in a clinical sample can be used to help identify the agent. In many cases the agent can be detected and identified in this way, even if it cannot be isolated or detected by immunologic means. New techniques and ad ...
RNA silencing
... RNA silencing• plant “immunity” to viral infection involves spread of signals across membranes • silencing triggered by dsRNA, transgenes, viruses • If virus carries a plant gene, that plant gene is also silenced • “Recovery”- when virus degrades the virus entirley, and is now protected against inf ...
... RNA silencing• plant “immunity” to viral infection involves spread of signals across membranes • silencing triggered by dsRNA, transgenes, viruses • If virus carries a plant gene, that plant gene is also silenced • “Recovery”- when virus degrades the virus entirley, and is now protected against inf ...
Lecture Notes: Bacteriophage
... (see textbook, Fig 19.4). After a complete + strand has been copied, CisA re-ligates the DNA, creating a circular ssDNA + strand. Early in infection, the replication cycle produces several RFs; late in infection, the only product of replication is progeny + strands that are packaged into viral capsi ...
... (see textbook, Fig 19.4). After a complete + strand has been copied, CisA re-ligates the DNA, creating a circular ssDNA + strand. Early in infection, the replication cycle produces several RFs; late in infection, the only product of replication is progeny + strands that are packaged into viral capsi ...
Lytic cycle
... -Nucleic acid core surrounded by capsid Nucleic acid can be DNA or RNA; Circular or linear; Single- or double-stranded Some viruses store specialized enzymes inside their capsids Many animal viruses have an envelope ...
... -Nucleic acid core surrounded by capsid Nucleic acid can be DNA or RNA; Circular or linear; Single- or double-stranded Some viruses store specialized enzymes inside their capsids Many animal viruses have an envelope ...
Virus quantification

Virus quantification involves counting the number of viruses in a specific volume to determine the virus concentration. It is utilized in both research and development (R&D) in commercial and academic laboratories as well as production situations where the quantity of virus at various steps is an important variable. For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus quantification to continually adapt and monitor the process in order to optimize production yields and respond to ever changing demands and applications. Examples of specific instances where known viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization and adaptation of methods to cell culture. This page discusses various techniques currently used to quantify viruses in liquid samples. These methods are separated into two categories, traditional vs. modern methods. Traditional methods are industry-standard methods that have been used for decades but are generally slow and labor-intensive. Modern methods are relatively new commercially available products and kits that greatly reduce quantification time. This is not meant to be an exhaustive review of all potential methods, but rather a representative cross-section of traditional methods and new, commercially available methods. While other published methods may exist for virus quantification, non-commercial methods are not discussed here.