Nucleic Acid Structures, Energetics, and Dynamics
... sequences occur in the target, a longer n-mer sequence can be used to resolve ambiguities. To implement the method, a complete set of 4n oligonucleotides of length n is immobilized on a solid support, and a target strand is presented. The target strand hybridizes by Watson-Crick base pairing to each ...
... sequences occur in the target, a longer n-mer sequence can be used to resolve ambiguities. To implement the method, a complete set of 4n oligonucleotides of length n is immobilized on a solid support, and a target strand is presented. The target strand hybridizes by Watson-Crick base pairing to each ...
M2 RNA Pol Ⅰ genes
... Eukaryotic promoters are extremely diverse and are difficult to characterize. They typically lie upstream of the gene and can have regulatory elements several kilobases away from the transcriptional start site. (Fig.11.19) ...
... Eukaryotic promoters are extremely diverse and are difficult to characterize. They typically lie upstream of the gene and can have regulatory elements several kilobases away from the transcriptional start site. (Fig.11.19) ...
nucleicacidchemistry
... sRNA: few bases / molecule often found in nucleus; thus it’s often called small nuclear RNA, snRNA Involved in various functions, including processing of mRNA in the spliceosome Some are catalytic Typically 20-1000 bases Not terribly plentiful: ~2 % of total RNA ...
... sRNA: few bases / molecule often found in nucleus; thus it’s often called small nuclear RNA, snRNA Involved in various functions, including processing of mRNA in the spliceosome Some are catalytic Typically 20-1000 bases Not terribly plentiful: ~2 % of total RNA ...
Supplemental Data
... and used for hybridizaiton to the cDNA macroarray. Normalized mean signal intensities from all time points after inoculation with Bgh or from the corresponding non-inoculated controls in three biological replicates were calculated and compared to each other per spotted unigene. ...
... and used for hybridizaiton to the cDNA macroarray. Normalized mean signal intensities from all time points after inoculation with Bgh or from the corresponding non-inoculated controls in three biological replicates were calculated and compared to each other per spotted unigene. ...
Geuvadis Analysis Meeting
... • Quantifications: do you want a normalization before uploading or is this in the responsibility of the analyzing group? • Quantifications: • Timeline for studies—main paper Oct-end of the year. • Separate publications possible if there is sufficient material for a separate story? • What would be th ...
... • Quantifications: do you want a normalization before uploading or is this in the responsibility of the analyzing group? • Quantifications: • Timeline for studies—main paper Oct-end of the year. • Separate publications possible if there is sufficient material for a separate story? • What would be th ...
Chapter 15: Genes and How They Work
... To find out how a eukaryotic cell uses its DNA to direct the production of particular proteins, you must first ask where in the cell the proteins are made. We can answer this question by placing cells in a medium containing radioactively labeled amino acids for a short time. The cells will take up t ...
... To find out how a eukaryotic cell uses its DNA to direct the production of particular proteins, you must first ask where in the cell the proteins are made. We can answer this question by placing cells in a medium containing radioactively labeled amino acids for a short time. The cells will take up t ...
Bacteriophage MS2 RNA
... permitted the direct comparison of genetic information and corresponding gene products (Fiers et al., 1976). One of the observations made possible by analysis of this sequence is that the choice of degenerate codons is clearly non-random (Fiers et al., 1971 and ...
... permitted the direct comparison of genetic information and corresponding gene products (Fiers et al., 1976). One of the observations made possible by analysis of this sequence is that the choice of degenerate codons is clearly non-random (Fiers et al., 1971 and ...
HUA1, a Regulator of Stamen and Carpel Identities
... weak ag-4 allele or heterozygous for the strong ag-1 allele). HUA2 was cloned previously and shown to code for a novel protein. We isolated the HUA1 gene using a map-based approach and show that it encodes a protein with six CCCHtype zinc finger motifs that is also found in yeast, Caenorhabditis ele ...
... weak ag-4 allele or heterozygous for the strong ag-1 allele). HUA2 was cloned previously and shown to code for a novel protein. We isolated the HUA1 gene using a map-based approach and show that it encodes a protein with six CCCHtype zinc finger motifs that is also found in yeast, Caenorhabditis ele ...
Infectious Hematopoietic Necrosis Virus genesig
... The test is positive so if you are only interested in obtaining a ‘present or absent’ answer for your sample then your result is secure as a positive test. However, the test contains an Internal Extraction Control component that identifies if the sample is of high quality. This quality control test ...
... The test is positive so if you are only interested in obtaining a ‘present or absent’ answer for your sample then your result is secure as a positive test. However, the test contains an Internal Extraction Control component that identifies if the sample is of high quality. This quality control test ...
Exercise 1: RNA
... should certainly check them out. Note, as we discovered today, because many of these tools are written by different authors, there are often errors or subtle incompatibilities, and it is often a case of either trial and error, googling error messages, or emailing the tool authors to solve these prob ...
... should certainly check them out. Note, as we discovered today, because many of these tools are written by different authors, there are often errors or subtle incompatibilities, and it is often a case of either trial and error, googling error messages, or emailing the tool authors to solve these prob ...
RNA gene prediction
... distributed on the tree of life, and does not reflect the diversity accordingly either. ...
... distributed on the tree of life, and does not reflect the diversity accordingly either. ...
Trans - Wiley
... • Spread efficiently into a homologous position in an allele that lacks the intron. • Movement is mediated by highly-specific homing endonucleases that are typically encoded by the self-splicing intron. ...
... • Spread efficiently into a homologous position in an allele that lacks the intron. • Movement is mediated by highly-specific homing endonucleases that are typically encoded by the self-splicing intron. ...
UCSC Known Genes (by Jim Kent)
... • Single exon gene edges take 4 though. • Rank input RNA by whether refSeq, and number of good edges they use. • If any good edges, output a transcript consisting of the edges used by the first RNA. • Output transcript based on next RNA if the good edges it uses have not been output in same order be ...
... • Single exon gene edges take 4 though. • Rank input RNA by whether refSeq, and number of good edges they use. • If any good edges, output a transcript consisting of the edges used by the first RNA. • Output transcript based on next RNA if the good edges it uses have not been output in same order be ...
Return to the RNAi world: rethinking gene expression and
... in the DNA sequence. The germline cells, which in C. elegans inherit PIE-1 protein, are the only cells that retain the potential to launch the developmental program again in the next generation. How do developing cells, all with the same DNA content, lock in different programs of gene expression tha ...
... in the DNA sequence. The germline cells, which in C. elegans inherit PIE-1 protein, are the only cells that retain the potential to launch the developmental program again in the next generation. How do developing cells, all with the same DNA content, lock in different programs of gene expression tha ...
Chapter 24: Promoters and Enhancers
... – An array short (<10 bp) sequence elements – also bind transcription factors – may be located several to many kb distant. ...
... – An array short (<10 bp) sequence elements – also bind transcription factors – may be located several to many kb distant. ...
Chapter 18 - Operons - Foothill Technology High
... The Lac operon controls the production of the ß-galactosidase, an enzyme that catalyzes the hydrolysis (break-down) of lactose into glucose and galactose. This is an inducible operon, meaning gene expression ß-galactosidase is stimulated by the presence of an co-inducer, lactose. ...
... The Lac operon controls the production of the ß-galactosidase, an enzyme that catalyzes the hydrolysis (break-down) of lactose into glucose and galactose. This is an inducible operon, meaning gene expression ß-galactosidase is stimulated by the presence of an co-inducer, lactose. ...
You may not start to read the questions printed on the subsequent
... the mutant monomer is "poisoning" any tetramer that it is part of A simple quantitative model would be that the inclusion of even a single mutant monomer makes the tetramer non-functional so only one in 16 tetramers would be jUnctional. This may lead to significant de-repression because of a greater ...
... the mutant monomer is "poisoning" any tetramer that it is part of A simple quantitative model would be that the inclusion of even a single mutant monomer makes the tetramer non-functional so only one in 16 tetramers would be jUnctional. This may lead to significant de-repression because of a greater ...
NOTE slides 15-21
... During what process must DNA replication occur? Why? What is cell division also known as in unicellular organisms? Explain how DNA structure allows for precise replication using the terms template and base pairing (review). Use the diagram (right) to describe replication of the single, circular prok ...
... During what process must DNA replication occur? Why? What is cell division also known as in unicellular organisms? Explain how DNA structure allows for precise replication using the terms template and base pairing (review). Use the diagram (right) to describe replication of the single, circular prok ...
Chapter Sixteen
... tRNA) present in a cell and what their roles in protein synthesis are. Understand the general process by which proteins are made in a cell: where it happens and how it happens. Understand the basic idea of the genetic code —that each amino acid is coded for by a sequence of three nucleotides (a codo ...
... tRNA) present in a cell and what their roles in protein synthesis are. Understand the general process by which proteins are made in a cell: where it happens and how it happens. Understand the basic idea of the genetic code —that each amino acid is coded for by a sequence of three nucleotides (a codo ...
DNA constructs designed to produce short hairpin, interfering RNAs
... than the 100 fold responses found by Bridge et al. (2003). Thus, an interferon response to highly expressing shRNA constructs may contribute to apparent early fetal lethality of these constructs. This interferon response could be related to the amount of shRNA generated by the transgene and it is ve ...
... than the 100 fold responses found by Bridge et al. (2003). Thus, an interferon response to highly expressing shRNA constructs may contribute to apparent early fetal lethality of these constructs. This interferon response could be related to the amount of shRNA generated by the transgene and it is ve ...
Gene Expression Microarray Analysis of Archival FFPE Samples
... frozen tumor tissue. Many laboratories are trying to develop methods to allow a similar degree of gene expression profiling using FFPE samples.3 FFPE samples represent the largest source of archival biological material available for large retrospective prognostic studies of human cancer, with over 4 ...
... frozen tumor tissue. Many laboratories are trying to develop methods to allow a similar degree of gene expression profiling using FFPE samples.3 FFPE samples represent the largest source of archival biological material available for large retrospective prognostic studies of human cancer, with over 4 ...
Human fetal normal cDNA panel
... Description: cDNA panel is comprised of 5 tubes each containing 10 μl of PCR Ready First Strand cDNAs from 5 tissues. Human fetal normal cDNA panels representing 6 systems of human fetal normal organs are available. Each panel contains from 4 different fetal normal organs and human placenta cDNA as ...
... Description: cDNA panel is comprised of 5 tubes each containing 10 μl of PCR Ready First Strand cDNAs from 5 tissues. Human fetal normal cDNA panels representing 6 systems of human fetal normal organs are available. Each panel contains from 4 different fetal normal organs and human placenta cDNA as ...
Infectious Bursal Disease Virus (IBDV) genesig
... genus Avibirnavirus of family Birnaviridae. There are two distinct serotypes of the virus, but only serotype 1 viruses cause disease in poultry. At least six antigenic subtypes of IBDV serotype 1 have been identified by in vitro cross-neutralization assay. Viruses belonging to one of these antigenic ...
... genus Avibirnavirus of family Birnaviridae. There are two distinct serotypes of the virus, but only serotype 1 viruses cause disease in poultry. At least six antigenic subtypes of IBDV serotype 1 have been identified by in vitro cross-neutralization assay. Viruses belonging to one of these antigenic ...
Transcription and Translation
... of your codon is ‘U’ Look at the top of the table where you see the title ...
... of your codon is ‘U’ Look at the top of the table where you see the title ...
RNA world
The RNA world refers to the self-replicating ribonucleic acid (RNA) molecules that were precursors to all current life on Earth. It is generally accepted that current life on Earth descends from an RNA world, although RNA-based life may not have been the first life to exist.RNA stores genetic information like DNA, and catalyzes chemical reactions like an enzyme protein. It may, therefore, have played a major step in the evolution of cellular life. The RNA world would have eventually been replaced by the DNA, RNA and protein world of today, likely through an intermediate stage of ribonucleoprotein enzymes such as the ribosome and ribozymes, since proteins large enough to self-fold and have useful activities would only have come about after RNA was available to catalyze peptide ligation or amino acid polymerization. DNA is thought to have taken over the role of data storage due to its increased stability, while proteins, through a greater variety of monomers (amino acids), replaced RNA's role in specialized biocatalysis.The RNA world hypothesis is supported by many independent lines of evidence, such as the observations that RNA is central to the translation process and that small RNAs can catalyze all of the chemical group and information transfers required for life. The structure of the ribosome has been called the ""smoking gun,"" as it showed that the ribosome is a ribozyme, with a central core of RNA and no amino acid side chains within 18 angstroms of the active site where peptide bond formation is catalyzed. Many of the most critical components of cells (those that evolve the slowest) are composed mostly or entirely of RNA. Also, many critical cofactors (ATP, Acetyl-CoA, NADH, etc.) are either nucleotides or substances clearly related to them. This would mean that the RNA and nucleotide cofactors in modern cells are an evolutionary remnant of an RNA-based enzymatic system that preceded the protein-based one seen in all extant life.Evidence suggests chemical conditions (including the presence of boron, molybdenum and oxygen) for initially producing RNA molecules may have been better on the planet Mars than those on the planet Earth. If so, life-suitable molecules, originating on Mars, may have later migrated to Earth via panspermia or similar process.