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Controlling Gene Expression
Controlling Gene Expression

... constitutive genes and are expressed by all cells.  Other genes are only needed by certain cells or at specific times. The expression of these inducible genes is tightly controlled in most cells.  For example, beta cells in the pancreas make the protein insulin by expressing the insulin gene. If n ...
Chapter 11 Powerpoint
Chapter 11 Powerpoint

... specific aa-tRNAs leads to synthesis of polypeptide with an ordered amino acid sequence ...
13.2 Ribosomes and Protein Synthesis
13.2 Ribosomes and Protein Synthesis

... ________________ are made by joining _____________________ together into long chains, called _________________________. As many as 20 different amino acids are commonly found in polypeptides. ...
Controling Gene Expression
Controling Gene Expression

... constitutive genes and are expressed by all cells.  Other genes are only needed by certain cells or at specific times. The expression of these inducible genes is tightly controlled in most cells.  For example, beta cells in the pancreas make the protein insulin by expressing the insulin gene. If n ...
Translation - My Teacher Pages
Translation - My Teacher Pages

... • There are no tRNA molecules that match the stop codons, but a special protein called a release factor does recognize the sequence, and attaches. • This special protein causes the newly created amino acid chain to be released. ...
Translation Initiation in E
Translation Initiation in E

... percent of CDSs use GUG and 1% UUG. Rarely AUA, ACG and CUG used. [Some abundant proteins (e.g., ribsomal proteins) use GUG as a start codon, and thus the argument that AUG is the strongest start codon does not seem to be true.] ...
Gene Section KIF14 (kinesin family member 14) Atlas of Genetics and Cytogenetics
Gene Section KIF14 (kinesin family member 14) Atlas of Genetics and Cytogenetics

... 274 aa kinesin motor domain containing an ATP-binding site (aa 447-454) which is involved in microtubule-dependent ATPase activity, and a microtubule binding site (aa 455-628) involved in ATP-dependent protein transport. The second is a 67 aa forkhead-associated (FHA) domain (aa 825-891) which has s ...
报告清单 - SIBCB
报告清单 - SIBCB

... cognate amino acid catalyzed by aminoacyl-tRNA synthetase with high levels of accuracy and efficiency. Crucial bases in tRNALeu to aminoacylation or editing functions of leucyl-tRNA synthetase have been extensively studied mainly by in vitro methods. In the present study, we constructed two Saccharo ...
2007b
2007b

... 1. Describe the properties of the general transcription factors of RNA polymerase II, I and III. What is TBP and what general transcription factors have TBP as a component? What enzymatic activities do some of the transcription factors possess and how do they facilitate transcription? Describe TAFs ...
Chapter 17 Practice Multple Choice
Chapter 17 Practice Multple Choice

... e. a discrete unit of hereditary information that consists of a sequence of amino acids ____ 23. The anticodon of a particular tRNA molecule is a. complementary to the corresponding mRNA codon. b. complementary to the corresponding triplet in rRNA. c. the part of tRNA that bonds to a specific amino ...
Chapter 18 - Operons - Foothill Technology High
Chapter 18 - Operons - Foothill Technology High

... operons, using proteins that inhibit or promote binding of RNA pol. – Distal and Proximal Control Elements – Proteins involved include: • Transcription factors • Activators • Mediator Proteins ...
Free Response 2009 - Page County Public Schools
Free Response 2009 - Page County Public Schools

... (b) Cells regulate both protein synthesis and protein activity. Discuss TWO specific mechanisms of protein regulation in eukaryotic cells. (c) The central dogma does not apply to some viruses. Select a specific virus or type of virus and explain how it • deviates from the central dogma. ...
Protein Synthesis
Protein Synthesis

... which genes will be expressed (used to make a protein). This can be affected by the cell’s history and/or environment (g+e=p) Proteins may be overproduced, underproduced or produced at incorrect times ...
Transcription and RNA Processing
Transcription and RNA Processing

... • Pol I - pre rRNA not known to be used by viruses • Pol II - makes mRNAs and some micro RNAs • Pol III - Adenovirus VA RNAs, EBV EBERs and some micro RNAs ...
Technical Journal Club: Intrabodies to Visualize Endogenous Proteins
Technical Journal Club: Intrabodies to Visualize Endogenous Proteins

... Transfected PSD95.FingRGFP into neurons of mouse embryos using in utero electroporation Expression was examined at 7 weeks of age from sections of perfused and fixed brains. Image E is from a live animal through a cranial window ...
Honors Biology
Honors Biology

... C. Overall Process of Protein Synthesis Label the structures on the diagram using the structures listed below. ...
Worksheet/Learning Aid Key
Worksheet/Learning Aid Key

... 1. Bacterial genes coding for individual proteins needed for the same cellular process are organized as operons that share the same non-coding regulatory DNA sequence known as the promoter region. 2. Each of the “operon” genes is known as a structural ...
Promega Notes: T4 RNA Ligase: A Molecular Tool for RNA and DNA
Promega Notes: T4 RNA Ligase: A Molecular Tool for RNA and DNA

... amino acid. This improvement greatly simplified the original anticodon loop replacement procedure, and they demonstrated that, while lacking post-transcriptional base modifications, the efficiency of amber suppression of the run-off suppressor tRNA was equivalent to a suppressor tRNA constructed by ...
splicing
splicing

... RNAi that targets the gene’s control region, a promoter-associated transcript) rather than the coding region • Another silencing mechanism targets nuclear RNA as endogenous double-stranded siRNAs that contain a nuclear localization signal can enter the nucleus and cause degradation of nuclear RNAs b ...
Differential Gene Expression in the Gastrula of Xenopus Laevis
Differential Gene Expression in the Gastrula of Xenopus Laevis

... Southern Blot using Eco-RI digest of Xenopus genomic DNA (Fig. 3)(in kb) ...
First week lectures
First week lectures

... enzymatic (machine-like) activity – Can be dangerous for the data to process itself! ...
Transcription/translation
Transcription/translation

... How do cells control Gene Expression?  For RNA Polymerase to do its job it has to attach to the DNA molecule ...
Molecular Testing and Clinical Diagnosis
Molecular Testing and Clinical Diagnosis

... • Two common detection systems have been developed. – On glass slides, hybridization can be detected by fluorescence and spot color detection by a microarray scanner. – The silicone chip consists of electrodes, independently addressable via an electronic control system. Hybridization is detected by ...
Mapping of the RNA-binding domain of the alfalfa mosaic virus
Mapping of the RNA-binding domain of the alfalfa mosaic virus

... virus (RCNMV) (Osman et al., 1992), the ORF2 (open reading frame 2) product of foxtail mosaic potexvirus (Rouleau et al., 1993) and the putative 17K movement protein of potato leafroll luteovirus (Tacke et al., 1993). The nucleic acid binding domain(s) have been localized in the movement proteins of ...
Gene Section HNRNPK (heterogeneous nuclear ribonucleoprotein K)
Gene Section HNRNPK (heterogeneous nuclear ribonucleoprotein K)

... The functions of hnRNPK are defined by its modular structure that allows it to interact with both nucleic acids and proteins. It has been suggested that hnRNPK serves as a docking platform that facilitates the interaction between the molecular partners involved in the processes that compose gene exp ...
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RNA-binding protein

RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes.RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others.They are cytoplasmic and nuclear proteins. However, since most mature RNA is exported from the nucleus relatively quickly, most RBPs in the nucleus exist as complexes of protein and pre-mRNA called heterogeneous ribonucleoprotein particles (hnRNPs).RBPs have crucial roles in various cellular processes such as: cellular function, transport and localization. They especially play a major role in post- transcriptional control of RNAs, such as: splicing, polyadenylation, mRNA stabilization, mRNA localization and translation. Eukaryotic cells encode diverse RBPs, approximately 500 genes, with unique RNA-binding activity and protein-protein interaction. During evolution, the diversity of RBPs greatly increased with the increase in the number of introns. Diversity enabled eukaryotic cells to utilize RNA exons in various arrangements, giving rise to a unique RNP (ribonucleoprotein) for each RNA. Although RBPs have a crucial role in post-transcriptional regulation in gene expression, relatively few RBPs have been studied systematically.
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