Fulltext - Jultika
... University of Oulu, during the years 2001–2008. I would like to express my deepest gratitude to my supervisor Professor Kalervo Hiltunen for his excellent guidance and constant support during this study. He shared with me his hand-on experiences from protein purification from animal tissues to commu ...
... University of Oulu, during the years 2001–2008. I would like to express my deepest gratitude to my supervisor Professor Kalervo Hiltunen for his excellent guidance and constant support during this study. He shared with me his hand-on experiences from protein purification from animal tissues to commu ...
Oxidation-Reduction Enzymes
... oxygen as described in the introduction; in addition it may act also as a peroxidase. It is present in greater or smaller amounts in all tissues (in structures called peroxisomes) and body fluids. Its major significance lies in decomposition of H2O2, harmful for the organism. In the present experime ...
... oxygen as described in the introduction; in addition it may act also as a peroxidase. It is present in greater or smaller amounts in all tissues (in structures called peroxisomes) and body fluids. Its major significance lies in decomposition of H2O2, harmful for the organism. In the present experime ...
Answer Key 2 - UC Davis Plant Sciences
... Why were rats fed with heptanoic acid (7:0) relatively healthy? (3 pts) Heptanoic acid is an odd-numbered fatty acid, which is degraded by β-oxidation into acetyl-CoA and propionyl-CoA. Unlike acetyl-CoA, propionyl-CoA can be converted to oxaloacetate (OAA), which is a precursor in gluconeogenesis a ...
... Why were rats fed with heptanoic acid (7:0) relatively healthy? (3 pts) Heptanoic acid is an odd-numbered fatty acid, which is degraded by β-oxidation into acetyl-CoA and propionyl-CoA. Unlike acetyl-CoA, propionyl-CoA can be converted to oxaloacetate (OAA), which is a precursor in gluconeogenesis a ...
Effects of dietary polyunsaturated fatty acids on
... The activity of COX was measured using the CYTOCOX1 kit from Sigma-Aldrich. As COX is only found on the inner mitochondrial membrane, its activity can be used as an indicator of inner mitochondrial membrane surface area (Leary et al., 2003). The colorimetric assay is based on the decrease in absorba ...
... The activity of COX was measured using the CYTOCOX1 kit from Sigma-Aldrich. As COX is only found on the inner mitochondrial membrane, its activity can be used as an indicator of inner mitochondrial membrane surface area (Leary et al., 2003). The colorimetric assay is based on the decrease in absorba ...
melatonin and succinate reduce rat liver mitochondrial dysfunction
... reduced glutathione decreased in red blood cells (by 25%, p<0.05) (Table 2). The liver mitochondrial protein thiol group content and mitochondrial total thiol group content did not change as a result of diabetes (Table 2). The level of PSSG increased in mitochondria of diabetic rats (by 50%, p<0.05) ...
... reduced glutathione decreased in red blood cells (by 25%, p<0.05) (Table 2). The liver mitochondrial protein thiol group content and mitochondrial total thiol group content did not change as a result of diabetes (Table 2). The level of PSSG increased in mitochondria of diabetic rats (by 50%, p<0.05) ...
Bax - Hypromatrix
... 0.2 µg/µl, store at 4 oC For research use only BACKGROUND Bax (for Bcl-associated X protein) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax has extensive ...
... 0.2 µg/µl, store at 4 oC For research use only BACKGROUND Bax (for Bcl-associated X protein) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax has extensive ...
Pavel Doležal
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
Pavel Doležal
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
Oxygen - CriticalCareMedicine
... The ratio of VO2/DO2 is the oxygen extraction ratio (ER). How can you calculate the ER without knowing the Hgb? The ER increases in conditions such as exercise, CHF, and anemia as a result of a lower CvO2. The converse occurs in sepsis. Each organ has its own metabolic needs so individual organ ER v ...
... The ratio of VO2/DO2 is the oxygen extraction ratio (ER). How can you calculate the ER without knowing the Hgb? The ER increases in conditions such as exercise, CHF, and anemia as a result of a lower CvO2. The converse occurs in sepsis. Each organ has its own metabolic needs so individual organ ER v ...
Some Aspects of Fatty Acid Oxidation in Isolated Fat
... These were either male Wistar rats obtained from A. Tuck and Son Ltd. (Rayleigh, Essex, U.K.), male Sprague-Dawley rats from Bantin and Kingman (Hull, U.K.) or were Wistar or Sprague-Dawley rats bred in the animal colony at University College London. Fed animals were maintained on cube diet 41B (Bru ...
... These were either male Wistar rats obtained from A. Tuck and Son Ltd. (Rayleigh, Essex, U.K.), male Sprague-Dawley rats from Bantin and Kingman (Hull, U.K.) or were Wistar or Sprague-Dawley rats bred in the animal colony at University College London. Fed animals were maintained on cube diet 41B (Bru ...
A strategically designed small molecule attacks alpha
... signaling molecules with the ability to modulate a number of cellular signaling processes, owing to their ability to modify proteins, including via oxidation of specific cysteine residues [1,2]. ROS have also been found to directly regulate key enzymes of matter/energy metabolism. The mitochondrial ...
... signaling molecules with the ability to modulate a number of cellular signaling processes, owing to their ability to modify proteins, including via oxidation of specific cysteine residues [1,2]. ROS have also been found to directly regulate key enzymes of matter/energy metabolism. The mitochondrial ...
A strategically designed small molecule attacks alpha-ketoglutarate dehydrogenase in tumor cells
... signaling molecules with the ability to modulate a number of cellular signaling processes, owing to their ability to modify proteins, including via oxidation of specific cysteine residues [1,2]. ROS have also been found to directly regulate key enzymes of matter/energy metabolism. The mitochondrial ...
... signaling molecules with the ability to modulate a number of cellular signaling processes, owing to their ability to modify proteins, including via oxidation of specific cysteine residues [1,2]. ROS have also been found to directly regulate key enzymes of matter/energy metabolism. The mitochondrial ...
When is the human soul infused
... did indeed exist even before fecundation. Embryologists of the day soon were maintaining that the “ embryo” in the unfertilized “ ovum ” possessed miniature human organs, even though the body was not yet living. The natural conclusion was that the sperm’s activity consisted in vivifying the pre-form ...
... did indeed exist even before fecundation. Embryologists of the day soon were maintaining that the “ embryo” in the unfertilized “ ovum ” possessed miniature human organs, even though the body was not yet living. The natural conclusion was that the sperm’s activity consisted in vivifying the pre-form ...
Cellular Respirationn Review Answers
... Statement: Women should not drink even small amounts of alcoholic beverages while pregnant. Student answers will vary. Some possible points that students may make include the following: • Because it is not possible to determine a safe amount of alcohol consumption during pregnancy, women should not ...
... Statement: Women should not drink even small amounts of alcoholic beverages while pregnant. Student answers will vary. Some possible points that students may make include the following: • Because it is not possible to determine a safe amount of alcohol consumption during pregnancy, women should not ...
pdf file
... mitochondria, decreased substantially the Ca2+ induced cytochrome c release, an early event of the apoptotic programme. It is possible that the different effects observed could arise in part from differences in the concentration range used. Furthermore, functions of mitochondria from various tissues ...
... mitochondria, decreased substantially the Ca2+ induced cytochrome c release, an early event of the apoptotic programme. It is possible that the different effects observed could arise in part from differences in the concentration range used. Furthermore, functions of mitochondria from various tissues ...
Cloning in Livestock Animal
... (TI) at a subsequent date, but sperm banking provides the assisted reproductive technologies (ART) couple with a backup option if a sample cannot be collected on demand or if sample quality is poor on the day eggs are available. In the case of donor insemination, samples are quarantined for 6 month ...
... (TI) at a subsequent date, but sperm banking provides the assisted reproductive technologies (ART) couple with a backup option if a sample cannot be collected on demand or if sample quality is poor on the day eggs are available. In the case of donor insemination, samples are quarantined for 6 month ...
Chapter 9. Cellular Respiration STAGE 1: Glycolysis
... also used by prokaryotes and yeasts that are facultative anaerobes. Fermentation is also used by your own muscles when you are working out strenuously and gas exchange is not happening fast enough to replenish ATP through oxidative phosphorylation. ...
... also used by prokaryotes and yeasts that are facultative anaerobes. Fermentation is also used by your own muscles when you are working out strenuously and gas exchange is not happening fast enough to replenish ATP through oxidative phosphorylation. ...
SFE1, a Constituent of the Fertilization Envelope
... First decision: 27 June 2000. Accepted: 26 July 2000. Q 2000 by the Society for the Study of Reproduction, Inc. ISSN: 0006-3363. http://www.biolreprod.org ...
... First decision: 27 June 2000. Accepted: 26 July 2000. Q 2000 by the Society for the Study of Reproduction, Inc. ISSN: 0006-3363. http://www.biolreprod.org ...
Mitochondria, Chloroplasts, and Peroxisomes
... reproduce within eukaryotic cells. Consistent with their similar symbiotic lifestyles, the genomic DNA sequences of Rickettsia and mitochondria suggest that they share a common ancestor, from which the genetic system of present-day mitochondria evolved. Mitochondrial genomes are usually circular DNA ...
... reproduce within eukaryotic cells. Consistent with their similar symbiotic lifestyles, the genomic DNA sequences of Rickettsia and mitochondria suggest that they share a common ancestor, from which the genetic system of present-day mitochondria evolved. Mitochondrial genomes are usually circular DNA ...
The Senescence?Related Mitochondrial/Oxidative Stress Pathway
... stress pathway still needs to be provided. Structural analyses of mitochondria within hESCs have demonstrated an immature network characterized by few organelles with poorly developed cristae with peri-nuclear polarization [20–22]. Studies of organelle number and functionality confirmed a low conten ...
... stress pathway still needs to be provided. Structural analyses of mitochondria within hESCs have demonstrated an immature network characterized by few organelles with poorly developed cristae with peri-nuclear polarization [20–22]. Studies of organelle number and functionality confirmed a low conten ...
Stryer An overview of the citric acid cycle
... Origin of mitochondria: the endosymbiont hypothesis The endosymbiont hypothesis suggests that mitochondria have evolved from anaerobic bacteria which were phagocytosed by eukaryote cells at the time oxygen appeared on earth, Similarities between mitochondria and bacteria include the presence of: • ...
... Origin of mitochondria: the endosymbiont hypothesis The endosymbiont hypothesis suggests that mitochondria have evolved from anaerobic bacteria which were phagocytosed by eukaryote cells at the time oxygen appeared on earth, Similarities between mitochondria and bacteria include the presence of: • ...
Chemiosmotic theory of oxidative phosphorylation. Inhibitors
... Bacteriorhodopsin protein that pumps protons when illuminated. When the vesicle is exposed to light, ATP is formed. ...
... Bacteriorhodopsin protein that pumps protons when illuminated. When the vesicle is exposed to light, ATP is formed. ...
Proteomic Mapping of Mitochondria in Living Cells
... Fig. 2. Specificity and depth of coverage of the mitochondrial matrix proteome. (A) Analysis of specificity. The left two columns show the fraction of proteins with prior mitochondrial annotation in the entire human proteome (column 1) and in our matrix proteome (column 2). The right two columns sho ...
... Fig. 2. Specificity and depth of coverage of the mitochondrial matrix proteome. (A) Analysis of specificity. The left two columns show the fraction of proteins with prior mitochondrial annotation in the entire human proteome (column 1) and in our matrix proteome (column 2). The right two columns sho ...
Free fatty acids regulate the uncoupling protein and alternative
... respiration in the presence of D T T and Pyr had an intermediary value, which could be explained by the presence of a small amount of endogenous F F A in the mitochondrial suspension. These observations clearly indicated an inhibitory effect of LA on the A O X activity stimulated by D T T and Pyr. T ...
... respiration in the presence of D T T and Pyr had an intermediary value, which could be explained by the presence of a small amount of endogenous F F A in the mitochondrial suspension. These observations clearly indicated an inhibitory effect of LA on the A O X activity stimulated by D T T and Pyr. T ...
Minimum length of homology in a donor DNA to facilitate
... Zinc-finger nucleases (ZFNs) can induce targeted mutations by causing double strand breaks at a specific location on a chromosome (Urnov et al., 2005). CMP-Neu5Ac hydroxylase (CMAH) is widely expressed on the endothelial cells of many mammals except humans (both of these genes are pseudo genes in hu ...
... Zinc-finger nucleases (ZFNs) can induce targeted mutations by causing double strand breaks at a specific location on a chromosome (Urnov et al., 2005). CMP-Neu5Ac hydroxylase (CMAH) is widely expressed on the endothelial cells of many mammals except humans (both of these genes are pseudo genes in hu ...