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embj201489478-sup-0013-fig
embj201489478-sup-0013-fig

... Supplementary Figure S3. AtNTR1 interacts directly with ILP1 and ilp1 shows similar phenotypes to atntr1. (A) ilp1 shows similar phenotypic defects to antr1 including lethality at elevated temperature and late flowering. WT Col-0, atntr1-1, atntr1-2, ilp1-1, ilp1-2, atntr1-1 complemented with WT cop ...
PPT
PPT

... Strong, easy to detect, but rare in population; may not be reflective of common disease. Also, hard to collect family data. ...
Amino acid specificity in translation
Amino acid specificity in translation

... binds in a cleft formed by two rRNA bases, presumably deriving stability via stacking interactions (Figure 2c). At first glance, the three amino-acid-binding pockets seem to be well suited for their required functions. TyrRS, like all aaRSs, uses its pocket to specifically select its cognate amino a ...
Note 20 - South Tuen Mun Government Secondary School
Note 20 - South Tuen Mun Government Secondary School

... Mendel’s first law / Law of segregation – a character is controlled by a pair of alleles, the alleles separate during gamete formation and recombines randomly in fertilization. Phenotype – the observable character Genotype – the genetic make up Homozygous – the two alleles of a gene is the same e.g. ...
classification
classification

Ribosomes slide on lysine-encoding homopolymeric A stretches
Ribosomes slide on lysine-encoding homopolymeric A stretches

... amino acids. When a gene is ‘switched on’, the DNA that makes up the gene is copied into messenger ribonucleic acid (or mRNA) molecules, composed of building blocks called nucleotides. There are four types of nucleotides in mRNA molecules—commonly referred to as A, C, G, and U—and a set of three nuc ...
Perturbations ofDrosophila aActinin Cause Muscle Paralysis
Perturbations ofDrosophila aActinin Cause Muscle Paralysis

... We found additional heterogeneity within the musclespecific exon when we investigated the sequence of larval a-actinin cDNA . Larval cDNA fragments that included the muscle-specific exon were noted to be larger than their nonmuscle and pupal muscle counterparts . We discovered that both larval and s ...
regulation of a bacteriophage t4 late gene, soc, which
regulation of a bacteriophage t4 late gene, soc, which

... the soc-coding sequence (see Figure 1). T h e 1050- and 1550-base early transcripts (marked with closed circles in Figure 3) found both in del(39-56)4 and in the 3 3 - 5 7 mutant were detected with the RsaI probe 2 but not with the RsaI probe 1 shown in Figure 4. Both transcripts were also detected ...
Hyper-eccentric structural genes in the mitochondrial genome of the
Hyper-eccentric structural genes in the mitochondrial genome of the

... Diplonemid mitochondria are considered to have very eccentric structural genes. Coding regions of individual diplonemid mitochondrial genes are fragmented into small pieces and found on different circular DNAs. Short RNAs transcribed from each DNA molecule mature through a unique RNA maturation proc ...
High-Throughput Neurotechnology
High-Throughput Neurotechnology

... Dust, debris, air bubbles, and bacteria may also enter the chip. 2. Capture of a single animal by the single aspiration channel. 3. Isolation of a single animal within the chamber by low-pressure washing of the channels to remove and recycle the rest of the nematodes. 4. Cleaning of channels by high ...
March 21, 1968, Number 12, Page Number 659
March 21, 1968, Number 12, Page Number 659

... f u l l y separated subunits will reaggregate when the magnesium concentration is • increased. The analogous role of magnesium in plant and animal systems cited and the generality ot this phenomenon suggest its fundamental importance in cellular function. Magnesium is further involved in protein syn ...
Maneeshi Prasad
Maneeshi Prasad

... Yang, J. et al. (2009) Nat. Neurosci. 12:113. ...
Protein synthesis: Twenty three amino acids and
Protein synthesis: Twenty three amino acids and

... has been known for many years that chinks can be found in the cell’s armor. For instance, the leucine analog 5′,5′,5′-trifluoroleucine was shown to be charged onto tRNA and incorporated into proteins by Escherichia coli [7], while Bacillus subtilis growth could be made dependent on the tryptophan an ...
2 -1 -2 -1 1 2 K
2 -1 -2 -1 1 2 K

... Seed contains representative members of the family Full alignment contains all members of the family as detected with HMM constructed from seen alignment ...
Regulatory sequences
Regulatory sequences

... and Y sequences conserved in all MHC-II, Ii, and HLA-DM promoters. RFX, X2BP, NF-Y, and an as yet undefined W-binding protein bind cooperatively to these sequences and assemble into a stable higher order nucleoprotein complex referred to here as the MHC-II enhanceosome. CIITA is tethered to the enha ...
Giant chromosomes
Giant chromosomes

... be incubated with a radioactive RNA probe. • Autoradiography can be used to visualize the precise location where the gene is being transcribed. ...
Allelic variation in normal human FBN1 expression in a family with
Allelic variation in normal human FBN1 expression in a family with

... Subsequent FISH analysis also revealed that the MFAP1 locus, encoding a microfibril associated protein, was deleted in this patient. A single MFAP1-specific cosmid probe signal was detected in 76 out of 100 interphase cells in comparison to two 15q telomere control signals (data not shown). Pulse ch ...
Directed Evolution of ATP Binding Proteins from a Zinc Finger
Directed Evolution of ATP Binding Proteins from a Zinc Finger

... The sequences of the clones discussed above are shown in Figure 3, and the multiple alignment reveals clear sequence patterns. The first random loop of every sequence begins with XVXH, where X is any amino acid. The second random loop of every sequence with the exception of clone 19 begins with XXCX ...
RNA Editing, ADAR1, and the Innate Immune Response
RNA Editing, ADAR1, and the Innate Immune Response

... in Aicardi–Goutières syndrome (AGS) [57], a severe autoimmune disease with early onset encephalopathy associated with a high level of IFN-α in the cerebrospinal fluid and upregulated ISG transcription [58–61]. A subtype of AGS was found to be associated with multiple mutations in the ADAR1 gene [57] ...
Ubiquinone
Ubiquinone

... • Synthesis of several proteins require this tRNA is interrupted. ...
Experimental Procedures for Grant Write-Up
Experimental Procedures for Grant Write-Up

... b. Background values: The background value provides a measure of the signal intensity resulting from autofluorescence of the array surface and nonspecific binding of target or stain molecules. The background values for all the arrays in one experiment should be within +25% of the overall average bac ...
Interaction of Sesbania Mosaic Virus Movement Protein
Interaction of Sesbania Mosaic Virus Movement Protein

... and dynamic process which involves functional contribution from many proteins of viral and host origin [2]. A number of RNA viruses have been identified which require an MP and two or more additional proteins for effective cell-to-cell spread of their genetic material. For example, in the case of po ...
lecture notes
lecture notes

... occur during the movement of the nuclei to the periphery. An hour after fertilization the embryo is composed of about 800 nuclei forming a monolayer around the periphery of the egg. During the next 30 min the nuclei undergo another 3 rounds of division to form nuclear cleavage cycle 14 embryos conta ...
Cellular Respiration
Cellular Respiration

... Lactic Acid Fermentation • The enzyme lactate dehydrogenase converts pyruvate into lactic acid and converts NADH into NAD+. • Usually blood can remove the lactate, however if this does not happen muscle fatigue results. ...
Pursuing DNA Catalysts for Protein Modification
Pursuing DNA Catalysts for Protein Modification

... surrounding structures of unrelated proteins.7−9 At present, such approaches are untenable for nucleic acid catalysts, and their prospects are uncertain in part because of poor current understanding of active-site structure and function. One-at-atime screening approaches are inherently unlikely to s ...
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