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calicin (S-13): sc-162627 - Santa Cruz Biotechnology
calicin (S-13): sc-162627 - Santa Cruz Biotechnology

... calicin (CCIN) is a 588 amino acid testis specific protein of the sperm head cytoskeleton. Found in the sperm head perinuclear theca, calicin localizes to the postacrosomal calyx and colocalizes with Actin in the acrosomal region of round spermatids. calicin’s ability to form tetramers and higher po ...
The Nucleotide and Derived Amino Acid
The Nucleotide and Derived Amino Acid

... apo-A-IV contained 376 residues. Throughout most of quence and organization of the docosapeptide units are reits length, human apo-A-IVwas found to contain mul- markably homologous to the tandemly repeated segments tiple tandem 22-residue repeated segments having am- found in apo-A-I (7,9),which sug ...
post-transcriptional gene silencing by double
post-transcriptional gene silencing by double

... has also been shown in fungi, Drosophila17, C. elegans18 and rodent fibroblasts19. Transgene silencing in the ascomycete fungus, Neurospora crassa, had been termed ‘quelling’ and occurs at the post-transcriptional level (BOX 1). Furthermore, silencing that has been triggered in a transgenic nucleus ...
Yeast whole-genome analysis of conserved regulatory motifs
Yeast whole-genome analysis of conserved regulatory motifs

... Highly Likely Modifications in State ...
G5. Strategies for Stabilization of Enzymes in Organic
G5. Strategies for Stabilization of Enzymes in Organic

... genetic modification. The most effective method to enhance enzyme stability is replacing free enzymes to immobilized forms. This immobilization creates stiffer structure of biocatalyst preventing them to and unfold enzymes and form malformations of its active sites. Furthermore, the method increases ...
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof

... of PspGI (Kd = 0.5 μM).31 Unfortunately, higher PspGI concentrations result in a significant tryptophan fluorescence background signal impeding these experiments. Nevertheless, the observation of a long-lived bright state, in principle, suggests that 2AP flipping by the enzyme may be directly monitored ...
Three-Dimensional Structure of Adenosylcobinamide Kinase
Three-Dimensional Structure of Adenosylcobinamide Kinase

... ABSTRACT: The X-ray structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) from Salmonella typhimurium has been determined to 2.3 Å resolution. This enzyme of subunit molecular weight 19 770 plays a central role in the assembly of the nucleotide loop for adeno ...
ribosome
ribosome

... Watching the Process The mRNA enters the ribosome. The ribosome will begin to read the mRNA one codon at a time. Click on the amino acid that will be 1st delivered to the ribosome? Hint: Examine the Genetic Code chart on your handout to find the 1st codon. Now that methionine is delivered, tRNA dro ...
How oncoproteins regulate gene expression
How oncoproteins regulate gene expression

... as yet un-identified genes; this would allow p53 to exert control over the tumour suppression pathway with or without microRNAs (He et al., 2009). ...
Stockholm University
Stockholm University

... effective for overexpression of membrane proteins [53-56, 61, 62]), there are also several examples where they have failed [63, 64]. Significantly, analyses of large data sets have failed to find a correlation between codon usage and overexpression levels of membrane proteins in E. coli and S. cerev ...
Cytoplasmic N-Terminal Protein Acetylation Is Required for Efficient
Cytoplasmic N-Terminal Protein Acetylation Is Required for Efficient

... those of wild-type plants (data not shown). Furthermore, in the progeny of all transformants, the wild-type phenotype cosegregated with the transgene. Database searches of the completely sequenced Arabidopsis genome revealed that At2g38130 is a single-copy gene. The predicted coding region is entire ...
July 2012 Volume 22 In This Issue Dazzling Diamond of Hope
July 2012 Volume 22 In This Issue Dazzling Diamond of Hope

... exons, to reflect the fact that they are what you see as final expressed portions of a gene. The intervening sequences between exons, so called introns, are rapidly removed and oftentimes degraded, and so seemed like a terribly wasteful way to organize genetic information. Rather than focus on the r ...
Exercise 11 - Understanding the Output for a blastn Search
Exercise 11 - Understanding the Output for a blastn Search

... relative of the model fruit fly Drosophila melanogaster) contains region(s) with sequence similarity to any known genes. The unknown sequence is an 11,000 base pair (bp) fragment of genomic DNA, and the objective of gene annotation is to find and precisely map the coding regions of any genes in this ...
emboj201294-sup
emboj201294-sup

... (A) Schematic representation of the generation of the Cdk7lox and Cdk7mut alleles from the Cdk7loxfrt allele present in ES clone D032B11 (German Gene Trap Consortium). (Top) Representation of the Cdk7loxfrt null allele generated by the insertion of the rFlipROSAbgeo gene-trap cassette within intron ...
Supplemental Material 1 Simultaneous isolation of mRNA, miRNA
Supplemental Material 1 Simultaneous isolation of mRNA, miRNA

... expressed as [(All MREs in transcript X for all miRs predicted to target X’s 3’UTR) - (# of miRs predicted to target X’s 3’UTR + 1)] / (MREs in X’s 3’UTR for all considered miRs). This criteria rewards transcripts for having a higher number of predicted MREs for each targeting miR. As the number of ...
Let`s see How Dramatic Effect can be drawn by Point Mutation with
Let`s see How Dramatic Effect can be drawn by Point Mutation with

... Introduction to our Project Movie Point mutation? Mutation with replacement of single base nucleotide in DNA or RNA, also with insertions or deletions of nucleotides. ...
Portfolio 2 - Biology2Nash
Portfolio 2 - Biology2Nash

... three basic parts: a base, a deoxyribose molecule, and a phosphate group. There are four different bases: adenine, cytosine, guanine, and thymine. Only one base is found in each nucleotide. Follow the directions. 1. In the diagram below, the sequence of nucleotides has the code AGCT. Color the diagr ...
1 - life.illinois.edu
1 - life.illinois.edu

... gene are often less polar than nonsense mutations near the 5’ end. This is likely because termination of translation in mRNA near the 3’ end of the gene occurs close to a ribosome binding site and AUG codon for the next downstream gene. Ribosomes can reinitiate translation and thereby protect the mR ...
Fatty Acids - Mayo Clinic
Fatty Acids - Mayo Clinic

... HIV-1 RNA quantitation is performed by PCR using the Roche Amplicor System. Plasma is chemically extracted and the viral RNA is precipitated with isopropanol. A known amount of a standard synthetic RNA molecule is added to each specimen to permit quantitation of HIV RNA by a comparison of resulting ...
Click to Add Text
Click to Add Text

... • 1 in 6 individual in US is affected from depression during their lifetime • Approximately 18.8 million American adults, or about 9.5 percent of the U.S. population age 18 and older in a given year, have a depressive disorder • Nearly twice as many women (12.0 percent) as men (6.6 percent) are affe ...
Translation Section 1 From Genes to Proteins Chapter 10
Translation Section 1 From Genes to Proteins Chapter 10

... • Both prokaryotic and eukaryotic cells are able to regulate which genes are expressed and which are not, depending on the cell’s needs. • The piece of DNA that overlaps the promoter site and serves as the on-off switch is called an operator. ...
Identification of a novel testis‐specific gene and its potential roles in
Identification of a novel testis‐specific gene and its potential roles in

... Aim: To identify and characterize a novel gene with potential roles in testis development and spermatogenesis. Methods: A cDNA microarray was constructed from a human testis large insert cDNA library and hybridized with probes of human or mouse adult and fetal testes. Differentially expressed genes ...
Characterization of transcription factor binding sites by
Characterization of transcription factor binding sites by

Nucleic acid crystallography: current progress
Nucleic acid crystallography: current progress

... observed base pairs are of the standard Watson–Crick type and the backbone between the third and the fourth nucleotide assumes a sharp kink. The structure probably provides just a glimpse at the potential complexity of DNA structure when non-canonical pairing modes are considered. Although DNA canno ...
143KB - NZQA
143KB - NZQA

... Merit, candidates who were awarded Achievement with Excellence commonly: • compared independent assortment and crossing over with the recombination process in linked genes • used well annotated diagrams showing how the linked genes could be separated by crossing over if they were far enough apart on ...
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