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Micro chpt. 9 notes
Micro chpt. 9 notes

... a. Assume that the right-hand side of the DNA molecule is where the strands separate, replication begins, and the replication fork moves from right-to-left. The leading strand would be copied from right-to-left (5’3’) in the same direction as the movement of the replication fork with use of the top ...
c-Myc co-ordinates mRNA cap methylation and ribosomal RNA
c-Myc co-ordinates mRNA cap methylation and ribosomal RNA

... mammals, the triphosphatase and guanylyltransferase are contained in one protein, CE/RNGTT (capping enzyme/RNA guanylyltransferase and 50 -triphosphatase). The methyltransferase, RNMT (RNA guanine-7 methyltransferase), catalyses mRNA cap methylation. RNMT has a cofactor, RAM (RNA-activating miniprot ...
Slide 1
Slide 1

... form the basal transcription complex to initiate transcription at a low frequency. For a gene to achieve a higher rate of mRNA synthesis, additional transcription factors must bind to other sites that can be near the promoter or quite distant. This is achieved by combinatorial control. Each factor r ...
ch 12 notes
ch 12 notes

... Mutations in the autosomal cells do not get passed on Mutations that occur in sex cells are passed on to the organism’s offspring and will be present in every cell of the offspring. ...
Genomes
Genomes

... These codons determine the beginning and end of the genetic code in an mRNA molecule (and so the beginning and end of a polypeptide) Components of Translation Template - mRNA tRNA - Picks up the appropriate amino acid floating in the cytoplasm and transports amino acids to the mRNA. tRNAs are transc ...
RNA Isolation and Technology Applications
RNA Isolation and Technology Applications

... • Multiple types and roles • Often permanently modified via splicing • Usually single-stranded • Intermolecular binding ...
DNA, RNA, Proteins
DNA, RNA, Proteins

... Another name for protein synthesis is _______________ translation ...
Glycolysis, Krebs Cycle, Electron Transport Chain
Glycolysis, Krebs Cycle, Electron Transport Chain

... Glycolysis (NADH only), and the Krebs Cycle. 27. In which phase of cellular respiration is water made? Electron Transport Chain 28. What would happen to the cellular respiration process if the enzyme (aka catalyst) for one step of the process was missing or defective? Entire process would stop. 29. ...
How to Study for the Anatomy and Physiology Prerequisite Exam:
How to Study for the Anatomy and Physiology Prerequisite Exam:

... Topics in Biology to Prepare for APE (Anatomy & Physiology Prerequisite Exam) based on Mader’s biology text Chapter 1: Introduction  Levels of Organization  Prokaryote vs Eukaryote Characteristics Chapter 2: Basic Chemistry  Elements most common in living organisms  Atomic Structure  Bonding: ...
DNA Review Sheet Answers
DNA Review Sheet Answers

... What does the TATA box do? A sequence of DNA found in Eukaryotes –marks the spot right before RNA polymerase will transcribe the desired gene ...
Library screening
Library screening

... poly(A) RNA fraction obtained from one sample is used as a template for the synthesis of the corresponding cDNA, which is then cloned into vector The cDNA library is then plated at a relatively low density to facilitate subsequent identification of individual clones by colonies or plaques hybridizat ...
A. DNA and Chromosomes
A. DNA and Chromosomes

... contains a complete copy of your DNA. Why, then, are some cells nerve cells with dendrites and axons, while others are red blood cells that have lost their nuclei and are packed with hemoglobin? Why are cells so different in structure and function? If the characteristics of a cell depend upon the pr ...
CST Review
CST Review

... 45. The gene for color vision (C) is dominant to the gene for color blindness (c) and is located on the X chromosome. If a color blind man and a woman with homozygous normal color vision have children, what are the chances that they will have a colorblind child? 46. Why do some lethal (deadly) allel ...
Cauliflower mosaic virus: still in the news
Cauliflower mosaic virus: still in the news

... et al., 2000). Recently, it has been shown that the function of P6 depends on its association with polysomes and the eukaryotic initiation factor eIF3 (Park et al., 2001). P6 physically interacts with the g subunit of eIF3 and three proteins of the 60S ribosomal subunit, namely L18 (Leh et al., 2000 ...
SAT II Protein Synthesis
SAT II Protein Synthesis

... 10. E, RNA is active during translation 11. The enzyme used in transcription is D, RNA polymerase 12. Protein synthesis takes place outside the nucleus in the cytoplasm or C 39. Use the base pairing rules to find the DNA (just include Ts not Us) so the answer should be D 40. mRNA does not have deoxy ...
RNA gene prediction
RNA gene prediction

... Biased and incomplete Database sequenced genomes are not evenly distributed on the tree of life, and does not reflect the diversity accordingly ...
DNA
DNA

... This preparation is then divided into four batches, and each is treated with a different replication-halting nucleotide (depicted here with a diamond shape), together with the four "usual" nucleotides. Each replication reaction then proceeds until a reactionterminating nucleotide is incorporated int ...
Transcription Translation 2017 p2.notebook
Transcription Translation 2017 p2.notebook

... a. facilitate the export of mRNA from nucleus b. protect mRNA from attack from cellular enzymes c. help ribosome bind to mRNA 4. RNA Splicing: process where introns are removed               and exons are adhered together a. intron: non coded regions b. exons: coding regions (will be translated) c.  ...
Gene Regulation Is Necessary
Gene Regulation Is Necessary

... Operons Operons are groups of genes that function to produce proteins needed by the cell. There are two different kinds of genes in operons: Structural genes code for proteins needed for the normal operation of the cell. For example, they may be proteins needed for the breakdown of sugars. The struc ...
Novel Function of the Eukaryotic Polypeptide
Novel Function of the Eukaryotic Polypeptide

... Abstract—The mammalian GTP-binding protein GSPT, whose carboxy-terminal sequence is homologous to the eukaryotic elongation factor EF1α, binds to the polypeptide chain releasing factor eRF1 to function as eRF3 in translation termination. However, the amino-terminal domain of GSPT, which contains a p ...
steroid hormone-97
steroid hormone-97

... estradiol HO ...
Microarray Protocol
Microarray Protocol

... harvest pool tracking form (see example on page 6) b) Place a conscious, non-anesthetized rat into a plastic restraint cone and decapitate, with the head falling directly into liquid nitrogen. Drain blood until muscle activity ceases and then open the rat at the midline. c) Remove and mince heart, l ...
Introduction to cDNA Microarray Technology complementary DNA
Introduction to cDNA Microarray Technology complementary DNA

... • Glass slides or similar supports containing cDNA sequences that serve as probes for measuring mRNA levels in target samples ...
PowerPoint
PowerPoint

... • Sequence length varies from a few hundred bases to a thousand or so. ...
Notes to Students:
Notes to Students:

... RNA processing questions (each question worth a total of 2 points; questions #4-5, each part worth one point) 1. Which answer best describes RNA processing? a. the process by which RNA is assembled from a DNA template b. the attraction of a binding protein and other transcription factors to tell the ...
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