The Formation of the Central Element of the
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
selection of antigens for antibody-based proteomics
... susceptible to degradation, and therefore careful handling of samples is required to not lower already weak protein signals further [Falk et al. 2007]. There is no equivalent to the polymerase chain reaction (PCR) for proteins, and without enrichment, the low signals are only detectable by a few sen ...
... susceptible to degradation, and therefore careful handling of samples is required to not lower already weak protein signals further [Falk et al. 2007]. There is no equivalent to the polymerase chain reaction (PCR) for proteins, and without enrichment, the low signals are only detectable by a few sen ...
Here. - Blumenstiel Lab
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
Structure of the Reovirus Membrane
... residues in two discontinuous polypeptide-chain segments (279–305 and 515–640). There are five ␣ helices (␣F–␣J) and a short  strand (25). Helices F, G, and J are an antiparallel bundle linking domains II and IV. Helices H and I and the exposed bridge between them project laterally, forming a crad ...
... residues in two discontinuous polypeptide-chain segments (279–305 and 515–640). There are five ␣ helices (␣F–␣J) and a short  strand (25). Helices F, G, and J are an antiparallel bundle linking domains II and IV. Helices H and I and the exposed bridge between them project laterally, forming a crad ...
Phosphoproteomic analysis of Arabidopsis thaliana Hanna Klang Årstrand
... identified 249 ribosomal protein genes that encode 32 small subunit (S) proteins and 48 large subunit (L) proteins, in total 80 ribosomal proteins (Barakat et al. 2001). The genes are organized into multigene families with two and up to seven members with an average of three family members. Many fam ...
... identified 249 ribosomal protein genes that encode 32 small subunit (S) proteins and 48 large subunit (L) proteins, in total 80 ribosomal proteins (Barakat et al. 2001). The genes are organized into multigene families with two and up to seven members with an average of three family members. Many fam ...
Noppl40 Shuttles on Tracks
... suggested that at least one of the identically repeated consensus sites is phosphorylated (Figure 3A, serine 567). This confirmed that CK II type phosphorylation indeed occurs in cellular Noppl40. Besides CK II sites, protein pattern analysis revealed 19 protein kinase C consensus sites, most of whi ...
... suggested that at least one of the identically repeated consensus sites is phosphorylated (Figure 3A, serine 567). This confirmed that CK II type phosphorylation indeed occurs in cellular Noppl40. Besides CK II sites, protein pattern analysis revealed 19 protein kinase C consensus sites, most of whi ...
ALL: What is diffusion? What are the consequences to molecules in
... ribosome How do the large & small subunits get together with the mRNA? How does it know where to start? How does it 'define' a codon? How does it know when to stop? How does it determine which amino acid to add? termination factor How does it know when to tell the ribosome to quit? How does it avoid ...
... ribosome How do the large & small subunits get together with the mRNA? How does it know where to start? How does it 'define' a codon? How does it know when to stop? How does it determine which amino acid to add? termination factor How does it know when to tell the ribosome to quit? How does it avoid ...
Presence and Absence of COX8 in Reptile Transcriptomes
... iguanids (Chameleo, Pogona), and three other reptiles (Tribolonotus, Gecko, Leioheterodon) (Figure 2, Table 2) while using the NCBI database we could find COX8 for other orders (Table 1). The COX8 coding contigs that were extracted were not highly conserved between the reptiles and mammals. For inst ...
... iguanids (Chameleo, Pogona), and three other reptiles (Tribolonotus, Gecko, Leioheterodon) (Figure 2, Table 2) while using the NCBI database we could find COX8 for other orders (Table 1). The COX8 coding contigs that were extracted were not highly conserved between the reptiles and mammals. For inst ...
Quantitative profiling of differentiation
... 2B. ICAT-labeled peptides were apparent as paired signals with a mass differential of 8 mass units for singly charged peptides. Numerous peptide peaks were detected, most of which appeared paired with another signal. The automated precursor ion selection afforded by the mass spectrometer, together w ...
... 2B. ICAT-labeled peptides were apparent as paired signals with a mass differential of 8 mass units for singly charged peptides. Numerous peptide peaks were detected, most of which appeared paired with another signal. The automated precursor ion selection afforded by the mass spectrometer, together w ...
J-Domain Protein CDJ2 and HSP70B Are a Plastidic Chaperone
... highly conserved J-domain that is responsible for the interaction with Hsp70. In addition, these cochaperones contain domains typical for protein–protein interactions, such as zinc finger or coiled-coil domains, by which specific substrates are bound (Cyr et al., 1994; Szabo et al., 1996; Miernyk, 200 ...
... highly conserved J-domain that is responsible for the interaction with Hsp70. In addition, these cochaperones contain domains typical for protein–protein interactions, such as zinc finger or coiled-coil domains, by which specific substrates are bound (Cyr et al., 1994; Szabo et al., 1996; Miernyk, 200 ...
Glucose Induces Lipolytic Cleavage of a Glycolipidic Plasma
... encoded by separate, although very similar genes, and may be expressed or processed differently in different cell types (for review see Low, 1989). In most cases the enzymic activities of the alternative forms of the proteins are very similar, and no specific difference has been found to correlate w ...
... encoded by separate, although very similar genes, and may be expressed or processed differently in different cell types (for review see Low, 1989). In most cases the enzymic activities of the alternative forms of the proteins are very similar, and no specific difference has been found to correlate w ...
CRONOS: the cross-reference navigation server
... In order to detect gene and protein names which are assigned to products of different genes and thus result in erroneous cross-references, dedicated lists are created for each organism separately. Organism-specific lists are necessary, since terms that are ambiguous in one organism might be explicit ...
... In order to detect gene and protein names which are assigned to products of different genes and thus result in erroneous cross-references, dedicated lists are created for each organism separately. Organism-specific lists are necessary, since terms that are ambiguous in one organism might be explicit ...
emerson - ShopRite
... At ShopRite, we think eating healthy should be affordable, fun, and tasty. That is why we are introducing Well Everyday! Well Everyday is a platform that will provide healthy inspiration, education, and a wide selection of delicious options to help you take small steps towards better nutrition. Wher ...
... At ShopRite, we think eating healthy should be affordable, fun, and tasty. That is why we are introducing Well Everyday! Well Everyday is a platform that will provide healthy inspiration, education, and a wide selection of delicious options to help you take small steps towards better nutrition. Wher ...
Document
... Ion channels and receptors - voltage-gated ion channels are membrane-bound proteins activated by change in transmembrane voltage - they are multi-subunit complexes with circular arrangement of identical or different proteins forming a pore region - it conducts specific species of ions such as Na+, ...
... Ion channels and receptors - voltage-gated ion channels are membrane-bound proteins activated by change in transmembrane voltage - they are multi-subunit complexes with circular arrangement of identical or different proteins forming a pore region - it conducts specific species of ions such as Na+, ...
Relationships between amino acid sequence and backbone torsion
... i) and PXYZ(i, i⫹1), of the configurations of each triplet XYZ. Here, PXYZ(i, i) is the probability of observing the middle residue (type Y) to be in state (i, i) and PXYZ(i, i⫹1) is the probability of observing residue type Y to be in state (i) and Z to be in state (i⫹1). PXYZ(i, i) is ...
... i) and PXYZ(i, i⫹1), of the configurations of each triplet XYZ. Here, PXYZ(i, i) is the probability of observing the middle residue (type Y) to be in state (i, i) and PXYZ(i, i⫹1) is the probability of observing residue type Y to be in state (i) and Z to be in state (i⫹1). PXYZ(i, i) is ...
Targeting of interleukin-2 to the periplasm of
... promotor regulated by the lacIQrepressor. The 420 bp EcoRI-Hind111 fragment of BBG3 replicative form (BBL) was inserted between the EcoRI and HindIII sites of pJF118EH to create pGF25 (Fig. 1). The ssOmpA-IL-2 fusion was created by linking the synthetic IL-2 gene to the signal peptide of OmpA by ins ...
... promotor regulated by the lacIQrepressor. The 420 bp EcoRI-Hind111 fragment of BBG3 replicative form (BBL) was inserted between the EcoRI and HindIII sites of pJF118EH to create pGF25 (Fig. 1). The ssOmpA-IL-2 fusion was created by linking the synthetic IL-2 gene to the signal peptide of OmpA by ins ...
Interaction of cycloheximide with 25S ribosomal RNA from yeast
... sulphate or by kethoxal. The sites of chemical modification and the drug protection site(s) are detected by isolating the ribosomal RNA and carrying out primer extension using suitable DNA oligomers that prime the action of the enzyme reverse transcriptase. After the above experiments had been carri ...
... sulphate or by kethoxal. The sites of chemical modification and the drug protection site(s) are detected by isolating the ribosomal RNA and carrying out primer extension using suitable DNA oligomers that prime the action of the enzyme reverse transcriptase. After the above experiments had been carri ...
Cellular Internalization of Fluorescent Proteins via Arginine
... layer between two outer hydrophilic surface in the membranes of plant cells to serve as a screening barrier for transport of materials across the biomembranes. Only specific biomolecules and ions may pass through via specific transporters or protein channels. Therefore, highly basic and hydrophilic ...
... layer between two outer hydrophilic surface in the membranes of plant cells to serve as a screening barrier for transport of materials across the biomembranes. Only specific biomolecules and ions may pass through via specific transporters or protein channels. Therefore, highly basic and hydrophilic ...
X-ray structure of the C-terminal domain of a coronavirus nucle
... nucleocapsid protein was expressed as before (Zhou, Williams et al. 1996). The protein was further purified by heparin affinity chromatography, concentrated to 1-2 mg/ml and was checked for monodispersity by dynamic light scattering (Dynapro) and negative stain electron microscopy. Limited proteolyt ...
... nucleocapsid protein was expressed as before (Zhou, Williams et al. 1996). The protein was further purified by heparin affinity chromatography, concentrated to 1-2 mg/ml and was checked for monodispersity by dynamic light scattering (Dynapro) and negative stain electron microscopy. Limited proteolyt ...
SDS-PAGE Sample Loading Buffer - G
... PREPARATION BEFORE USE The SDS may precipitate at cooler temperatures. Simply warm in a 37°C waterbath or incubator to redissolve the SDS. 2X SDS-PAGE SAMPLE LOADING BUFFER PROTOCOL ...
... PREPARATION BEFORE USE The SDS may precipitate at cooler temperatures. Simply warm in a 37°C waterbath or incubator to redissolve the SDS. 2X SDS-PAGE SAMPLE LOADING BUFFER PROTOCOL ...
The Dock and Lock Method: A Novel
... C-DDD1-Fab-hMN-14, was generated by linking the DDD1 peptide sequence, which is composed of amino acids 1 to 44 of human RIIa, to the COOH-terminal end of the Fd chain via a 14-residue flexible peptide linker (Fig. 2B). This construct was modified by incorporation of a cysteine residue adjacent to t ...
... C-DDD1-Fab-hMN-14, was generated by linking the DDD1 peptide sequence, which is composed of amino acids 1 to 44 of human RIIa, to the COOH-terminal end of the Fd chain via a 14-residue flexible peptide linker (Fig. 2B). This construct was modified by incorporation of a cysteine residue adjacent to t ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.