Expect Values
... when you really want the whole of two sequences to be aligned, without truncation. GAP could completely bypass a region of high local homology, if a better (or even just as good) path can be found in a different way. This is problematic if one short sequence is aligned against a longer one with inte ...
... when you really want the whole of two sequences to be aligned, without truncation. GAP could completely bypass a region of high local homology, if a better (or even just as good) path can be found in a different way. This is problematic if one short sequence is aligned against a longer one with inte ...
Training for Endurance and Strength: Lessons from Cell Signaling
... exercise results in an increase in muscle mass, and endurance exercise results in an increase in muscle capillary density, mitochondrial protein, fatty acid-oxidation enzymes, and more metabolically efficient forms of contractile and regulatory proteins. In the 25 yr since Hickson's initial descript ...
... exercise results in an increase in muscle mass, and endurance exercise results in an increase in muscle capillary density, mitochondrial protein, fatty acid-oxidation enzymes, and more metabolically efficient forms of contractile and regulatory proteins. In the 25 yr since Hickson's initial descript ...
Proteins and Enzymes
... Evolution of proteins • Some pre-RNA polymer is believed to have come first; RNA evolved from that • RNA can function as enzymes • RNA became the template for DNA • Amino acids are attracted to codons • RNA catalyzed condensation of amino acids • Ribosomes evolved to increase efficiency and ...
... Evolution of proteins • Some pre-RNA polymer is believed to have come first; RNA evolved from that • RNA can function as enzymes • RNA became the template for DNA • Amino acids are attracted to codons • RNA catalyzed condensation of amino acids • Ribosomes evolved to increase efficiency and ...
Central Dogma of Molecular Biology: The way of diverting
... number of atoms known in the universe). ...
... number of atoms known in the universe). ...
Prediction of Anti-parallel and Parallel Beta
... in accuracy in general and 80-85% for predicting helix and coils. The major bottleneck lies in predicting beta-sheets, which involves long-range interactions between regions of the protein chain that are not necessarily consecutive in the primary sequence. Therefore designing an effective algorithm ...
... in accuracy in general and 80-85% for predicting helix and coils. The major bottleneck lies in predicting beta-sheets, which involves long-range interactions between regions of the protein chain that are not necessarily consecutive in the primary sequence. Therefore designing an effective algorithm ...
The Structure and Function of Large Biological Molecules
... Plants generally transport carbohydrates from leaves to roots ...
... Plants generally transport carbohydrates from leaves to roots ...
Protein Synthesis I
... iii. Only about ½ of the proteins we make get folded- one reason is that there is a 10-20% failure of synthetases to put the right amino acid on the right tRNA 1. So not all proteins are synthesized properly 2. That is one reason why it is more economical to make quaternary structures out of small n ...
... iii. Only about ½ of the proteins we make get folded- one reason is that there is a 10-20% failure of synthetases to put the right amino acid on the right tRNA 1. So not all proteins are synthesized properly 2. That is one reason why it is more economical to make quaternary structures out of small n ...
![HydF as a scaffold protein in [FeFe] hydrogenase H](http://s1.studyres.com/store/data/022194833_1-d80dffc73c0d81a42175e12a27187746-300x300.png)
HydF as a scaffold protein in [FeFe] hydrogenase H
... These results, which demonstrate the ability of purified HydFEG to activate HydADEFG in the absence of any other proteins or small molecules, support our hypothesis that HydF serves as a scaffold for assembly of a cluster that is subsequently transferred to HydADEFG, thus converting it to an active ho ...
... These results, which demonstrate the ability of purified HydFEG to activate HydADEFG in the absence of any other proteins or small molecules, support our hypothesis that HydF serves as a scaffold for assembly of a cluster that is subsequently transferred to HydADEFG, thus converting it to an active ho ...
Robustness of the model
... proteins. Using a p-value cut-off of 0.05, only coiled-coil proteins (p-value 0.005) and uncharacterized proteins (p-value 0.015) significantly changed the network when opisthokont-specific proteins were removed (Table S5). Due to the strong individual signal of uncharacterized proteins, all but one ...
... proteins. Using a p-value cut-off of 0.05, only coiled-coil proteins (p-value 0.005) and uncharacterized proteins (p-value 0.015) significantly changed the network when opisthokont-specific proteins were removed (Table S5). Due to the strong individual signal of uncharacterized proteins, all but one ...
A target site for template-based design of measles virus entry inhibitors
... stretch of the fusion protein (F protein) ectodomain, the fusion peptide, into the target cell membrane. Further conformational rearrangements in the F protein ectodomain ultimately result in merging of the two membranes (14). To date, a crystal structure of a paramyxovirus fusion protein trimer is ...
... stretch of the fusion protein (F protein) ectodomain, the fusion peptide, into the target cell membrane. Further conformational rearrangements in the F protein ectodomain ultimately result in merging of the two membranes (14). To date, a crystal structure of a paramyxovirus fusion protein trimer is ...
2 Nucleic Acids
... that speed up chemical reactions in cells. Other proteins are antibodies, which bind to foreign substances such as bacteria and target them for destruction. Still other proteins carry messages or transport materials. For example, human red blood cells contain a protein called hemoglobin, which binds ...
... that speed up chemical reactions in cells. Other proteins are antibodies, which bind to foreign substances such as bacteria and target them for destruction. Still other proteins carry messages or transport materials. For example, human red blood cells contain a protein called hemoglobin, which binds ...
on the nature of the "non-saturable" migration of
... fusion. If it were practical to test solute levels at still higher levels, saturability might well become evident; but of course a slowing of the relative uptake rate at very high levels might result from the replacement of the environmental Na + or C1- by the amino acid, from effects on water distr ...
... fusion. If it were practical to test solute levels at still higher levels, saturability might well become evident; but of course a slowing of the relative uptake rate at very high levels might result from the replacement of the environmental Na + or C1- by the amino acid, from effects on water distr ...
Qproteome Nuclear Protein Handbook
... cell biologists. The identification of nuclear proteins — especially nucleic-acid– binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s ge ...
... cell biologists. The identification of nuclear proteins — especially nucleic-acid– binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s ge ...
can detect white spot syndrome virus (wssv
... Early attempts to generate antibodies against purified WSSV proved to be difficult because the virus was always damaged during purification. Therefore, expressed recombinant protein was utilized as an antigen. In order to obtain a high protein yield in E. coli, a truncated version of the protein lac ...
... Early attempts to generate antibodies against purified WSSV proved to be difficult because the virus was always damaged during purification. Therefore, expressed recombinant protein was utilized as an antigen. In order to obtain a high protein yield in E. coli, a truncated version of the protein lac ...
Proteins - virtual laboratories
... The answer is DNA-dependent RNA polymerase, which we will refer to as RNA polymerase. In bacteria, groups of genes share regulatory sequences recognized by specific σ factors. As we will see this makes it possible to regulate groups of specific genes in a coordinated manner. Now let us turn to how, ...
... The answer is DNA-dependent RNA polymerase, which we will refer to as RNA polymerase. In bacteria, groups of genes share regulatory sequences recognized by specific σ factors. As we will see this makes it possible to regulate groups of specific genes in a coordinated manner. Now let us turn to how, ...
Bioinorganic motifs: towards functional classification of metalloproteins
... (i) In coordination chemistry, the atoms or chemical groups bound to the central atom (usually a metal) via dative bond are called ligands. The donors of one or more electron pairs to the central atom are called monodentate or polydentate ligands, respectively. In bioinorganic chemistry, the ligands ...
... (i) In coordination chemistry, the atoms or chemical groups bound to the central atom (usually a metal) via dative bond are called ligands. The donors of one or more electron pairs to the central atom are called monodentate or polydentate ligands, respectively. In bioinorganic chemistry, the ligands ...
Biological Molecules: Water and Carbohydrates
... during this reaction. During a hydrolysis reaction a________ breaks up to form ________. Water is ______ during this reaction. ...
... during this reaction. During a hydrolysis reaction a________ breaks up to form ________. Water is ______ during this reaction. ...
Wool Tech. and Sheep Breed. 50
... a USDA Romanov/Suffolk flock (K. Leymaster) and the Roslin Institute Scottish Blackface flocks (S. Bishop). Only heterozygous sheep inheriting the gene from their sire (polar overdominance) express muscular hypertrophy (Georges et al. 1996), so the callipyge gene would be difficult to use commercial ...
... a USDA Romanov/Suffolk flock (K. Leymaster) and the Roslin Institute Scottish Blackface flocks (S. Bishop). Only heterozygous sheep inheriting the gene from their sire (polar overdominance) express muscular hypertrophy (Georges et al. 1996), so the callipyge gene would be difficult to use commercial ...
Production of Polyclonal Antibodies against Sucrose Transporter
... consisted of one cycle at 94 oC for 2 min, 30 cycles at 94 o C for 30 s, 55 oC for 60 s and 72 oC for 2 min, and a final extension step at 72 oC for 7 min. The amplified product was analyzed by 1% agarose gel electrophoresis. Recombinant plasmids were extracted from two clones using the Roche High P ...
... consisted of one cycle at 94 oC for 2 min, 30 cycles at 94 o C for 30 s, 55 oC for 60 s and 72 oC for 2 min, and a final extension step at 72 oC for 7 min. The amplified product was analyzed by 1% agarose gel electrophoresis. Recombinant plasmids were extracted from two clones using the Roche High P ...
UBIQUITIN AT FOX CHASE
... transfers Ub to the -NH2 of lysine of proteins. To determine the substrate specificity of this system Dr. Pickart found that a variety of small primary amines at much higher concentration are also very good Ub acceptors. The Ub-amides were good substrates for the previously purified Ub-thiolesteras ...
... transfers Ub to the -NH2 of lysine of proteins. To determine the substrate specificity of this system Dr. Pickart found that a variety of small primary amines at much higher concentration are also very good Ub acceptors. The Ub-amides were good substrates for the previously purified Ub-thiolesteras ...
Surface-activated Dynabeads
... A comprehensive selection of Dynabeads® is available. Some Dynabeads® are precoupled with specific ligands (e.g., streptavidin, protein A or G, antibodies, etc.). Other Dynabeads® have a specific surface chemistry for ligand coupling. We are also able to work with our customers to develop and custom ...
... A comprehensive selection of Dynabeads® is available. Some Dynabeads® are precoupled with specific ligands (e.g., streptavidin, protein A or G, antibodies, etc.). Other Dynabeads® have a specific surface chemistry for ligand coupling. We are also able to work with our customers to develop and custom ...
effects of insulin and anchorage on hepatocytic protein metabolism
... effect is potentiated by intermediate concentrations of amino acids, but is largely abolished at high amino acid concentrations which suppress degradation maximally, suggesting that the hormone may exert its effect indirectly by acting upon the more basal amino acid control mechanism. Glucagon, whic ...
... effect is potentiated by intermediate concentrations of amino acids, but is largely abolished at high amino acid concentrations which suppress degradation maximally, suggesting that the hormone may exert its effect indirectly by acting upon the more basal amino acid control mechanism. Glucagon, whic ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.