Caldicellulosiruptor tāpirins bind to crystalline cellulose! ! 1 Discrete
... ABSTRACT A variety of catalytic and non-catalytic protein domains are deployed by select microorganisms to deconstruct lignocellulose. These extracellular proteins are used to attach to, modify, and hydrolyze the complex polysaccharides present in plant cell walls. Cellulolytic enzymes, often contai ...
... ABSTRACT A variety of catalytic and non-catalytic protein domains are deployed by select microorganisms to deconstruct lignocellulose. These extracellular proteins are used to attach to, modify, and hydrolyze the complex polysaccharides present in plant cell walls. Cellulolytic enzymes, often contai ...
Chapter 3 - McGraw Hill Higher Education
... that are insoluble in water. • Fats and oils are well-known lipids used for energy storage and other purposes. • Phospholipids are components of the membranes that surround cells. • Steroids, which have a different structure from most lipids, are used as hormones and for other purposes. ...
... that are insoluble in water. • Fats and oils are well-known lipids used for energy storage and other purposes. • Phospholipids are components of the membranes that surround cells. • Steroids, which have a different structure from most lipids, are used as hormones and for other purposes. ...
Molecular Characterization of Two Arabidopsis Ire1 Homologs
... conformation or that eliminates them via a degradation after transport to the vacuole, or in the cytosol, after retrotranslocation across the ER membrane (for review, see Bonifacino and Weissman, 1998; Mori, 2000). When stress causes protein folding to be slowed, the presence of unfolded proteins in ...
... conformation or that eliminates them via a degradation after transport to the vacuole, or in the cytosol, after retrotranslocation across the ER membrane (for review, see Bonifacino and Weissman, 1998; Mori, 2000). When stress causes protein folding to be slowed, the presence of unfolded proteins in ...
CELLULAR ADAPTATION TO AMINO ACID AVAILABILITY:
... D. Ron’s group has identified a signalling pathway for regulating gene expression in mammals that is homologous to the wellcharacterised yeast general control response to amino acid deprivation (Harding et al. 2000). Its components include (figure 3) the mammalian homologue of the GCN2 kinase, the i ...
... D. Ron’s group has identified a signalling pathway for regulating gene expression in mammals that is homologous to the wellcharacterised yeast general control response to amino acid deprivation (Harding et al. 2000). Its components include (figure 3) the mammalian homologue of the GCN2 kinase, the i ...
Extracting quantitative information from
... starting points • Anders’ starting point this Monday: – Let’s say that we want to study life at the protein level – what technologies do we have at hand? ...
... starting points • Anders’ starting point this Monday: – Let’s say that we want to study life at the protein level – what technologies do we have at hand? ...
RbcL | Rubisco large subunit, form I and form II (50 µl) product
... AS07 218 | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein AS01 017S | anti-RbcL | Rubisco protein standard for quantitative western blot or positive control AS07 259 | anti-RbcS | Rubisco small subunit (SSU), rabbit antibody AS07 222 | RbcS | Rubisco small subunit (SSU) from ...
... AS07 218 | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein AS01 017S | anti-RbcL | Rubisco protein standard for quantitative western blot or positive control AS07 259 | anti-RbcS | Rubisco small subunit (SSU), rabbit antibody AS07 222 | RbcS | Rubisco small subunit (SSU) from ...
PDF - Oxford Academic - Oxford University Press
... protein as a molecular probe is whether it behaves biologically as the native protein. To check that point, we compared the relative association with polysomes of hStaufen-TAP, expressed by transfection of plasmid pChStaufen-TAP into 293T cells, and the endogenous hStaufen isoforms. The results are ...
... protein as a molecular probe is whether it behaves biologically as the native protein. To check that point, we compared the relative association with polysomes of hStaufen-TAP, expressed by transfection of plasmid pChStaufen-TAP into 293T cells, and the endogenous hStaufen isoforms. The results are ...
Quantitative Host Cell Protein Analysis Using Two
... of the insoluble constituent of the hydrolyzed Rapigest. The supernatant was reduced to dryness via vacuum centrifugation. Dried peptides were reconstituted in 500 μL of 0.1% formic acid and analyzed using a Waters 2695 Alliance HPLC instrument with UV detection for first dimension separation. Peptid ...
... of the insoluble constituent of the hydrolyzed Rapigest. The supernatant was reduced to dryness via vacuum centrifugation. Dried peptides were reconstituted in 500 μL of 0.1% formic acid and analyzed using a Waters 2695 Alliance HPLC instrument with UV detection for first dimension separation. Peptid ...
CHAPTER 1 PATHOGENESIS OF GOUSIEKTE 1.1
... Actin is one of the most conserved eukaryotic proteins and actin isoforms show greater than 90 % overall sequence homology, except in their 18 N-terminal residues (Lessard, 1988). The main actin in the heart is α-actin. The thin filament proteins tropomyosin (TPM) and the globular Ca2+-binding tropo ...
... Actin is one of the most conserved eukaryotic proteins and actin isoforms show greater than 90 % overall sequence homology, except in their 18 N-terminal residues (Lessard, 1988). The main actin in the heart is α-actin. The thin filament proteins tropomyosin (TPM) and the globular Ca2+-binding tropo ...
Finding a way to the nucleus - Purdue University
... to T-strands in the plant, thus protecting the DNA from nucleolytic destruction [9,10]. The complex of T-strands covalently linked to VirD2 and coated by VirE2 molecules is termed the ‘T-complex’ [11]. Although there are strong genetic and in vitro binding data indicating the existence of the T-comp ...
... to T-strands in the plant, thus protecting the DNA from nucleolytic destruction [9,10]. The complex of T-strands covalently linked to VirD2 and coated by VirE2 molecules is termed the ‘T-complex’ [11]. Although there are strong genetic and in vitro binding data indicating the existence of the T-comp ...
Effects of calf serum on pinocytosis and proteolysis in rat yolk sacs
... Intermediate concentrations of serum resulted in a quantity of tissue-associated radioactivity between these two extremes. The progressive accumulation of radioactivity by yolk sacs incubated in the presence of 50% serum indicates that lysosomal proteolysis, rather than pinocytosis, is ratelimiting ...
... Intermediate concentrations of serum resulted in a quantity of tissue-associated radioactivity between these two extremes. The progressive accumulation of radioactivity by yolk sacs incubated in the presence of 50% serum indicates that lysosomal proteolysis, rather than pinocytosis, is ratelimiting ...
Initiation of Innate Immune Responses in the
... only a few species of gram-negative bacteria and are bacteriostatic rather than bacteriolytic. However, until now no glycine-rich molecules have been reported in mammals. Some of this group antibacterial peptides such as apidaecin, gloverins, and attacins have an immediate effect on protein synthesi ...
... only a few species of gram-negative bacteria and are bacteriostatic rather than bacteriolytic. However, until now no glycine-rich molecules have been reported in mammals. Some of this group antibacterial peptides such as apidaecin, gloverins, and attacins have an immediate effect on protein synthesi ...
Coupling Coherence Distinguishes Structure Sensitivity in Protein
... We suggest that, in the multiple-pathway regime, the evolutionary linkage between the specific protein fold and the ET rate is likely to be weak: In this regime, RDA determines tunneling propensity. In the single-pathway large C regime, however, ET kinetics and protein structure are strongly linked. ...
... We suggest that, in the multiple-pathway regime, the evolutionary linkage between the specific protein fold and the ET rate is likely to be weak: In this regime, RDA determines tunneling propensity. In the single-pathway large C regime, however, ET kinetics and protein structure are strongly linked. ...
Proteomics methods for subcellular proteome analysis
... thereby increasing the reproducibility while being costeffective and time-effective. However, such kits are often limited with regard to what organelles can be enriched. Free flow electrophoresis is a highly versatile technology that was introduced nearly 50 years ago for the separation of a wide va ...
... thereby increasing the reproducibility while being costeffective and time-effective. However, such kits are often limited with regard to what organelles can be enriched. Free flow electrophoresis is a highly versatile technology that was introduced nearly 50 years ago for the separation of a wide va ...
Exploration of the Dynamic Properties of Protein Complexes
... to each other. Interactions within protein complexes are modulated through regulatory inputs that alter interactions and introduce new components and deplete existing components through exchange. While it is clear that the structure and function of any given protein complex is coupled to its dynamic ...
... to each other. Interactions within protein complexes are modulated through regulatory inputs that alter interactions and introduce new components and deplete existing components through exchange. While it is clear that the structure and function of any given protein complex is coupled to its dynamic ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.