Engineering and Identifying Supercharged Proteins
... Most medicines are small molecules, chemical compounds containing less than 100 atoms. For many diseases, however, small-molecule-based therapies have not been found. Recent efforts to discover bioactive molecules including human therapeutics have increasingly focused on macromolecules—for the purp ...
... Most medicines are small molecules, chemical compounds containing less than 100 atoms. For many diseases, however, small-molecule-based therapies have not been found. Recent efforts to discover bioactive molecules including human therapeutics have increasingly focused on macromolecules—for the purp ...
Leishmania Flagellum
... the characterization of individual elements. Bin/Amphiphysin/Rvs (BAR) domains are 200-amino-acid modular elements found in many eukaryotic multi-domain proteins [14]. Although their amino acid sequence may be poorly conserved and therefore difficult to identify, their structure is well conserved th ...
... the characterization of individual elements. Bin/Amphiphysin/Rvs (BAR) domains are 200-amino-acid modular elements found in many eukaryotic multi-domain proteins [14]. Although their amino acid sequence may be poorly conserved and therefore difficult to identify, their structure is well conserved th ...
Nuclear Transport of Plant Potyviral Proteins
... p.i. and remained at relatively constant or slightly decreased levels for an additional 3 days (data not shown). lmmunocytochemical analysis of TEV-infected tissue revealed virus activity in most cells by 3 days p.i. (see below), suggesting that accumulation between days 3 and 5 was due to increases ...
... p.i. and remained at relatively constant or slightly decreased levels for an additional 3 days (data not shown). lmmunocytochemical analysis of TEV-infected tissue revealed virus activity in most cells by 3 days p.i. (see below), suggesting that accumulation between days 3 and 5 was due to increases ...
The Ubiquitin System for Protein Degradation and Some of Its Roles
... reproduces energy-dependent protein degradation in the test tube, essential for the biochemical analysis of this system. For this purpose, we tried different sources, such as liver homogenates and extracts from cultured cells, and even from bacteria. We did not have any success in any of these attem ...
... reproduces energy-dependent protein degradation in the test tube, essential for the biochemical analysis of this system. For this purpose, we tried different sources, such as liver homogenates and extracts from cultured cells, and even from bacteria. We did not have any success in any of these attem ...
Characterization of the pscC
... fourth leading cause of nosocomial infection and is associated with cystic fibrosis; burn ...
... fourth leading cause of nosocomial infection and is associated with cystic fibrosis; burn ...
Entropy in Biology -R-ES-O-N-A-N-C-E--I-s-e-p-te-m-b-e-r--2-0-0
... protein unfolding reaction can exist in a larger number of equivalent states, compared with the native state, entropy increases during the unfolding reaction. From the simple formula defined by Ludwig Boltzmann in 1877, the entropy of the unfolded state, which is equal to kIn W (where k is the Boltz ...
... protein unfolding reaction can exist in a larger number of equivalent states, compared with the native state, entropy increases during the unfolding reaction. From the simple formula defined by Ludwig Boltzmann in 1877, the entropy of the unfolded state, which is equal to kIn W (where k is the Boltz ...
Uniikki kuitu
... RNAfold reads RNA sequences from stdin and calculates their minimum free energy (mfe) structure, partition function (pf) and base pairing probability matrix. It returns the mfe structure in bracket notation, its energy, the free energy of the thermodynamic ensemble and the frequency of the mfe struc ...
... RNAfold reads RNA sequences from stdin and calculates their minimum free energy (mfe) structure, partition function (pf) and base pairing probability matrix. It returns the mfe structure in bracket notation, its energy, the free energy of the thermodynamic ensemble and the frequency of the mfe struc ...
nitrogen fixing bacteria
... Living thing require nutrient cycles ( nitrogen cycle) because Some organism ( decomposers) obtain energy from the break down of dead bodies and waste ...
... Living thing require nutrient cycles ( nitrogen cycle) because Some organism ( decomposers) obtain energy from the break down of dead bodies and waste ...
BIOLOGY
... the synthesis of the protein on the ribosome. These post-translational modifications change the size and the structure of the final protein. Some modifications occur after a protein is made; others occur during translation of the protein, and are required for proper folding of the protein. One possi ...
... the synthesis of the protein on the ribosome. These post-translational modifications change the size and the structure of the final protein. Some modifications occur after a protein is made; others occur during translation of the protein, and are required for proper folding of the protein. One possi ...
Are There Beans and Peas On Your Plate?
... Are There Beans and Peas On Your Plate? Most Americans do not eat many dry beans and peas but there are great health benefits from these foods. Dry beans and peas are low in fat and high in fiber. When you eat them the fiber can help you feel full for a while. This may make it easier to manage your ...
... Are There Beans and Peas On Your Plate? Most Americans do not eat many dry beans and peas but there are great health benefits from these foods. Dry beans and peas are low in fat and high in fiber. When you eat them the fiber can help you feel full for a while. This may make it easier to manage your ...
Engineered tRNA suppression of a CFTR nonsense mutation
... mutation at the site of the original PTC (Roy, Friesen et al. 2016). Therefore, when therapeutically assisted PTC stop codon read-through is successful, the non-selective incorporation of an amino acid at the location of the nonsense codon has the potential to affect protein folding, trafficking and ...
... mutation at the site of the original PTC (Roy, Friesen et al. 2016). Therefore, when therapeutically assisted PTC stop codon read-through is successful, the non-selective incorporation of an amino acid at the location of the nonsense codon has the potential to affect protein folding, trafficking and ...
Review Article
... the early work on secretion centred on higher animals, both for reasons of medical interest and for the fact that some specialized tissues are essentially devoted to secretion of hormones or digestive enzymes and thus provide a rich source of active experimental material. Micro-organisms secrete sev ...
... the early work on secretion centred on higher animals, both for reasons of medical interest and for the fact that some specialized tissues are essentially devoted to secretion of hormones or digestive enzymes and thus provide a rich source of active experimental material. Micro-organisms secrete sev ...
BMC Microbiology
... Based on the sequence of the original 363 bp PCR product, gene-specific primers and nested gene-specific primers were designed for RACE as described in Methods. The 5' and 3' ends of the sscmk1 gene were obtained from two different RACE reactions each. PCR reactions were performed to confirm both th ...
... Based on the sequence of the original 363 bp PCR product, gene-specific primers and nested gene-specific primers were designed for RACE as described in Methods. The 5' and 3' ends of the sscmk1 gene were obtained from two different RACE reactions each. PCR reactions were performed to confirm both th ...
Thermodynamic and transport studies on some basic amino acids in
... aqueous sodium butyrate solution. Required amount of water and sodium acetate were taken to prepare at 0.4 m (molality) of solution in a clean dry conical flask with ground stopper. The required amount of amino acids for a given molality was dissolved and similar procedure has been adopted for diffe ...
... aqueous sodium butyrate solution. Required amount of water and sodium acetate were taken to prepare at 0.4 m (molality) of solution in a clean dry conical flask with ground stopper. The required amount of amino acids for a given molality was dissolved and similar procedure has been adopted for diffe ...
Screening for increased protein thiol oxidation in oxidatively
... variety of antioxidant systems to remove reactive oxygen species (ROS) generated from a variety of sources. Disruption of this oxidative balance, often referred to as oxidative stress, is evident in many chronic disease states including muscle wasting conditions and may contribute to the pathology [ ...
... variety of antioxidant systems to remove reactive oxygen species (ROS) generated from a variety of sources. Disruption of this oxidative balance, often referred to as oxidative stress, is evident in many chronic disease states including muscle wasting conditions and may contribute to the pathology [ ...
Capillary Electrophoresis System
... Capillary Electrophoresis (CE) is a well-established technique to separate and quantify proteins or other macromolecules (such as DNA, RNA) from complex mixtures. It also allowed the measurement of anions in solutions which can be interesting for example to determine the protein binding to metallic ...
... Capillary Electrophoresis (CE) is a well-established technique to separate and quantify proteins or other macromolecules (such as DNA, RNA) from complex mixtures. It also allowed the measurement of anions in solutions which can be interesting for example to determine the protein binding to metallic ...
Cell-Free Protein Expression Systems
... Rabbit Reticulocyte Lysate (RRL), Nuclease-Treated, is optimized for mRNA translation by the addition of several supplements. These include hemin, which prevents activation of the heme-regulated eIF-2a kinase; an energy-generating system consisting of phosphocreatine kinase and phosphocreatine; and ...
... Rabbit Reticulocyte Lysate (RRL), Nuclease-Treated, is optimized for mRNA translation by the addition of several supplements. These include hemin, which prevents activation of the heme-regulated eIF-2a kinase; an energy-generating system consisting of phosphocreatine kinase and phosphocreatine; and ...
SAPS(1) USER COMMANDS SAPS(1) NAME saps
... Output is directed to standard output. To run SAPS on the sequence file HMCU_DROME, for example (see above), one might type the command `saps HMCU_DROME | more' or `saps HMCU_DROME > OUTPUT'. The output format can be modified by the flags -d, -t, or -v, and -T: -d ...
... Output is directed to standard output. To run SAPS on the sequence file HMCU_DROME, for example (see above), one might type the command `saps HMCU_DROME | more' or `saps HMCU_DROME > OUTPUT'. The output format can be modified by the flags -d, -t, or -v, and -T: -d ...
Phosphoproteomics as a tool to unravel plant
... receptors. The analysis of plasma membrane phosphoproteins also showed that regions surrounding the phosphorylation sites can often be grouped into conserved phosphorylation motifs that may be targeted by similar kinases. Such analyses will accelerate the identification of the responsible kinase tar ...
... receptors. The analysis of plasma membrane phosphoproteins also showed that regions surrounding the phosphorylation sites can often be grouped into conserved phosphorylation motifs that may be targeted by similar kinases. Such analyses will accelerate the identification of the responsible kinase tar ...
Department of Chemistry and Clinical Biochemistry
... 2.1. Student is expected to attend all scheduled seminars and laboratory classes, and be punctual for classes. 2.2. For seminars, a student is obliged to present two topics during semester, related to the subject of the seminar, as an oral presentation for 10-15 minutes, granted 0-5 points. List of ...
... 2.1. Student is expected to attend all scheduled seminars and laboratory classes, and be punctual for classes. 2.2. For seminars, a student is obliged to present two topics during semester, related to the subject of the seminar, as an oral presentation for 10-15 minutes, granted 0-5 points. List of ...
Kidney function test
... Renal function tests • Glomerular function tests: all the clearance tests (innulin, creatinine, urea) • Tubular function test: urine concentration or dilution test or urine acidification test • Analysis of blood/serum: blood urea, serum creatinine, protein and electrolytes • Urine examination: simp ...
... Renal function tests • Glomerular function tests: all the clearance tests (innulin, creatinine, urea) • Tubular function test: urine concentration or dilution test or urine acidification test • Analysis of blood/serum: blood urea, serum creatinine, protein and electrolytes • Urine examination: simp ...
Synapse Specificity Minireview and Long
... exposure of the peripheral synapses to 5-HT, these results indicate that synapses need not be tagged or active to receive the proteins required for long-term synaptic plasticity. One might argue, however, that the application of 5-HT to sensory neuron somata “short circuits” the normal signaling pat ...
... exposure of the peripheral synapses to 5-HT, these results indicate that synapses need not be tagged or active to receive the proteins required for long-term synaptic plasticity. One might argue, however, that the application of 5-HT to sensory neuron somata “short circuits” the normal signaling pat ...
Manipulation of the host translation initiation complex eIF4F by DNA
... In the absence of their own translational machinery, all viruses must gain access to host cell ribosomes to synthesize viral proteins and replicate. Ribosome recruitment and scanning of capped host mRNAs is facilitated by the multisubunit eIF (eukaryotic initiation factor) 4F, which consists of a ca ...
... In the absence of their own translational machinery, all viruses must gain access to host cell ribosomes to synthesize viral proteins and replicate. Ribosome recruitment and scanning of capped host mRNAs is facilitated by the multisubunit eIF (eukaryotic initiation factor) 4F, which consists of a ca ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.