Manipulation of the host translation initiation complex eIF4F by DNA
... In the absence of their own translational machinery, all viruses must gain access to host cell ribosomes to synthesize viral proteins and replicate. Ribosome recruitment and scanning of capped host mRNAs is facilitated by the multisubunit eIF (eukaryotic initiation factor) 4F, which consists of a ca ...
... In the absence of their own translational machinery, all viruses must gain access to host cell ribosomes to synthesize viral proteins and replicate. Ribosome recruitment and scanning of capped host mRNAs is facilitated by the multisubunit eIF (eukaryotic initiation factor) 4F, which consists of a ca ...
Comparison of Trypsin Immobilization Techniques With or Without a
... The first stage in peptide mapping consists of chemical or enzymatic cleavage of a protein into specific peptides in order to obtain its fingerprint. To address the need for higher throughput in proteomics, fast enzymatic digestions and efficient analysis techniques like capillary electrophoresis (C ...
... The first stage in peptide mapping consists of chemical or enzymatic cleavage of a protein into specific peptides in order to obtain its fingerprint. To address the need for higher throughput in proteomics, fast enzymatic digestions and efficient analysis techniques like capillary electrophoresis (C ...
Amino acid substitutions that specifically impair the transcriptional
... Brd4(L). As can be been seen in Figs. 2A and B, Brd4(L) was indeed detected in the eluates from the GST-E2 TAD column but not those from control columns. Similar experiments were then performed with the E2 TAD from HPV31. For this type also, the eluate from the column containing the GST-E2 TAD conta ...
... Brd4(L). As can be been seen in Figs. 2A and B, Brd4(L) was indeed detected in the eluates from the GST-E2 TAD column but not those from control columns. Similar experiments were then performed with the E2 TAD from HPV31. For this type also, the eluate from the column containing the GST-E2 TAD conta ...
Recent advances in technology for measuring and manipulating cell
... GFPs as donor and acceptor. For example, activation of protein kinase Cα eventually results in autophosphorylation of Thr250, for which a phosphospecific antibody has been generated. Such autophosphorylation could be detected in live COS-7 cells by FRET from a GFP that has been fused to the PKC, to ...
... GFPs as donor and acceptor. For example, activation of protein kinase Cα eventually results in autophosphorylation of Thr250, for which a phosphospecific antibody has been generated. Such autophosphorylation could be detected in live COS-7 cells by FRET from a GFP that has been fused to the PKC, to ...
Proteomic analyses of the time course responses of mice infected
... Brucellosis is a major zoonotic disease caused by pathogens of the genus Brucella. The eradication of brucellosis in domestic animals, associated with the prevention of human infection, can be attained through accurate diagnosis. However, the conventional serological diagnosis of brucellosis has lim ...
... Brucellosis is a major zoonotic disease caused by pathogens of the genus Brucella. The eradication of brucellosis in domestic animals, associated with the prevention of human infection, can be attained through accurate diagnosis. However, the conventional serological diagnosis of brucellosis has lim ...
Gene7-06
... pool of free ribosomes that are in equilibrium with separate small and large subunits. 7. A ribosome can carry two aminoacyl-tRNAs simultaneously: its P site is occupied by a polypeptidyltRNA, which carries the polypeptide chain synthesized so far, while the A site is used for entry by an aminoacyl- ...
... pool of free ribosomes that are in equilibrium with separate small and large subunits. 7. A ribosome can carry two aminoacyl-tRNAs simultaneously: its P site is occupied by a polypeptidyltRNA, which carries the polypeptide chain synthesized so far, while the A site is used for entry by an aminoacyl- ...
IvDimitrov_slides
... Conclusions 1. An alignment-free method for in silico prediction of allergens based on the main physicochemical properties of proteins was developed. 2. The method uses z descriptors for representation of amino acids in the protein sequences and ACC transformation for conversion of proteins into ...
... Conclusions 1. An alignment-free method for in silico prediction of allergens based on the main physicochemical properties of proteins was developed. 2. The method uses z descriptors for representation of amino acids in the protein sequences and ACC transformation for conversion of proteins into ...
The protein import apparatus of chloroplasts
... indicate a common reeeptor for most of the bulk protein import or at least for the precursor proteins' listed above. which represent major chloroplast constituents of different localization. Though the influence of the synthetic peptides on the import pathway seems to be specific, it is not known wh ...
... indicate a common reeeptor for most of the bulk protein import or at least for the precursor proteins' listed above. which represent major chloroplast constituents of different localization. Though the influence of the synthetic peptides on the import pathway seems to be specific, it is not known wh ...
chromatography - Bio-Rad
... A human serum sample was loaded onto a BioLogic DuoFlow chromatography system configured to use both an Affi-Gel Blue and an Affi-Gel protein A column for removal of albumin and IgG, respectively (Figure 1). Since protein A media can be costly, the relative purification efficiency achieved using dif ...
... A human serum sample was loaded onto a BioLogic DuoFlow chromatography system configured to use both an Affi-Gel Blue and an Affi-Gel protein A column for removal of albumin and IgG, respectively (Figure 1). Since protein A media can be costly, the relative purification efficiency achieved using dif ...
Chemical genetics to chemical genomics: small
... This journal is ß The Royal Society of Chemistry 2005 ...
... This journal is ß The Royal Society of Chemistry 2005 ...
Protein Targeting into the Complex Plastid of Cryptophytes
... al. 2000). This cytoplasm, the periplastidal compartment, is devoid of typical eukaryotic compartments, e.g., mitochondrion and Golgi apparatus, but still harbors 80S ribosomes and a pigmy nucleus, the nucleomorph (Maier et al. 2000, Douglas et al. 2001; Cavalier-Smith 2002; Gilson and McFadden 2002 ...
... al. 2000). This cytoplasm, the periplastidal compartment, is devoid of typical eukaryotic compartments, e.g., mitochondrion and Golgi apparatus, but still harbors 80S ribosomes and a pigmy nucleus, the nucleomorph (Maier et al. 2000, Douglas et al. 2001; Cavalier-Smith 2002; Gilson and McFadden 2002 ...
Protein Structure Prediction
... been ( = = 180o). The second model is build up by constructed by taking all the peptide residues in the extended conformation assigning the peptide residues the , angles of the secondary structure states predicted by PSIPRED. The third model has been constructed with , angles corresponding ...
... been ( = = 180o). The second model is build up by constructed by taking all the peptide residues in the extended conformation assigning the peptide residues the , angles of the secondary structure states predicted by PSIPRED. The third model has been constructed with , angles corresponding ...
The RNA origin of transfer RNA aminoacylation and beyond
... respectively. P3 shown in box with question mark is a putative pair region. Proposed bases that form Watson–Crick pairs in r24min (G43 –U45) and tRNAs (A73 –C75) are underlined and the red-dashed arrow indicates their interactions. Putative bases involving the constitution of the Phe-binding site ar ...
... respectively. P3 shown in box with question mark is a putative pair region. Proposed bases that form Watson–Crick pairs in r24min (G43 –U45) and tRNAs (A73 –C75) are underlined and the red-dashed arrow indicates their interactions. Putative bases involving the constitution of the Phe-binding site ar ...
Proti-Ace Kit - Hampton Research
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
12.4 G Protein–Coupled Receptors and Second Messengers
... P –Tyr residues in IRS-1 serve as binding sites for proteins with SH2 domains. Some of these proteins, such as Grb2, have two or more protein-binding domains and can serve as adaptors that bring two proteins into proximity. ...
... P –Tyr residues in IRS-1 serve as binding sites for proteins with SH2 domains. Some of these proteins, such as Grb2, have two or more protein-binding domains and can serve as adaptors that bring two proteins into proximity. ...
Detection of plum pox potyviral protein–protein interactions in planta
... In previous studies, protein interaction maps of different potyviruses have been generated using yeast two-hybrid (YTH) systems, and these maps have demonstrated a high diversity of interactions of potyviral proteins. Using an optimized bimolecular fluorescence complementation (BiFC) system, a compl ...
... In previous studies, protein interaction maps of different potyviruses have been generated using yeast two-hybrid (YTH) systems, and these maps have demonstrated a high diversity of interactions of potyviral proteins. Using an optimized bimolecular fluorescence complementation (BiFC) system, a compl ...
Plastid-Targeting Peptides from the
... LASTIDS, the light-harvesting organelles of plants and algae, are the product of an ancient symbiosis between a cyanobacterium and a non-photosynthetic eukaryote. This process is referred to as primary endosymbiosis, and has given rise to the plastids of green algae and land plants, red algae and gl ...
... LASTIDS, the light-harvesting organelles of plants and algae, are the product of an ancient symbiosis between a cyanobacterium and a non-photosynthetic eukaryote. This process is referred to as primary endosymbiosis, and has given rise to the plastids of green algae and land plants, red algae and gl ...
Fractionation of rice glutelin polypeptides using gel filtration
... 29KD marker. Many of the fractions collected for subsequent SDS-PAGE in this study were closely spaced, and, therefore, a certain degree of component overlap was expected. However, the elution patternsfrom gel filtration in 0.5% SDS showed a less defined separation of the dissociated acidic polypept ...
... 29KD marker. Many of the fractions collected for subsequent SDS-PAGE in this study were closely spaced, and, therefore, a certain degree of component overlap was expected. However, the elution patternsfrom gel filtration in 0.5% SDS showed a less defined separation of the dissociated acidic polypept ...
Setting sail for glucose homeostasis with the AKAP150PP2Banchor
... b cell-specific AKAP150KO mice secrete less insulin upon glucose stimulation despite increased insulin content in the b cell that occurs as an adaptation to the impaired glucose tolerance. These mice clearly exhibited an impaired glucose tolerance that is due to defective insulin secretion because th ...
... b cell-specific AKAP150KO mice secrete less insulin upon glucose stimulation despite increased insulin content in the b cell that occurs as an adaptation to the impaired glucose tolerance. These mice clearly exhibited an impaired glucose tolerance that is due to defective insulin secretion because th ...
FYVE-dependent endosomal targeting of an arrestin-related
... In this report, we chose to focus on the protein AdcA from D. discoideum (Figure 1A–B). Arrestin-domain containing proteins extended with a FYVE domain were also found in D. purpureum, Polysphondylium pallidum, Entamoeba histolytica and E. dispar, other members of the Conosea group within the Amoebo ...
... In this report, we chose to focus on the protein AdcA from D. discoideum (Figure 1A–B). Arrestin-domain containing proteins extended with a FYVE domain were also found in D. purpureum, Polysphondylium pallidum, Entamoeba histolytica and E. dispar, other members of the Conosea group within the Amoebo ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.