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Bacterial Genetics
Bacterial Genetics

... 4. SOS response: induce many genes 5. Error-prone repair: fill in gaps with random sequences Thymine-thymine dimer formed by UV radiation ...
Document
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... • Chromosomes are composed of DNA and protein - the DNA is the genetic material. • Nucleic acid structure gives important insight into genetic function. ...
ERT 101 Biochemistry
ERT 101 Biochemistry

... 2. Compare between DNA and RNA ...
Session 4 - OpenWetWare
Session 4 - OpenWetWare

... depending on the location and size of the DNA and the type of cells being used, the basic steps are the same: break open the cells, purify the DNA, and re-suspend the DNA. ...
DNA stucture - worldofbiology09
DNA stucture - worldofbiology09

... contain all the necessary instructions (genes), DNA must have the ability to replicate itself. DNA replication is said to be semi-conservative as each new DNA molecule contains one strand from the original and one newly synthesised ...
Day 58 - upwardsapbio
Day 58 - upwardsapbio

... new DNA strand is created from the parent strand. There are many enzymes and proteins that aid in this complex process. After the many enzymes have created this new, semiconservative strand of DNA, it must then be proofread and repaired. The final, completed strand of DNA has about 1 in 10 billion n ...
Modes of Prokaryotic Genetic Exchange
Modes of Prokaryotic Genetic Exchange

... The phage can take up any DNA that is about the same size as it’s genome. ...
10 Worksheet 9 Handout for powerpoint Applying our Knowledg
10 Worksheet 9 Handout for powerpoint Applying our Knowledg

... • Involve inserting a proper working gene into the cell that lack the ability to produce their own protein • Insulin, inserting that gene into pancreatic cells • Need to use a vector to insert the gene, like a virus, which is site specific to pancreatic cells • Once there the virus would insert the ...
15.3_Applications_of_Genetic_Engineering
15.3_Applications_of_Genetic_Engineering

... Patient’s cells are then infected with the genetically engineered virus Virus will insert the healthy gene into the target cell and correct the defect. ...
Ch 15 Genetic Engineering
Ch 15 Genetic Engineering

... Patient’s cells are then infected with the genetically engineered virus Virus will insert the healthy gene into the target cell and correct the defect. ...
DNA Structure and Replication
DNA Structure and Replication

... – Enzymes break bonds between bases – Complementary strands separate – Complementary bases are added to strands – Copy of DNA results ...
Chapter 13 PowerPoint
Chapter 13 PowerPoint

... loop. Two replication forks begin at a single site, known as the origin of replication. Replication occurs in opposite directions until the forks meet on the other side of the loop. Eukaryotic cell replication starts at many sites along the chromosome. ...
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... Give the genotype and phenotype of the offspring. Genotype is what letters you use to represent a gene, such as Tt, or tt. Phenotype is what the baby would look like physically (tall, short, etc) 10. Mitosis is used to produce somatic cells, meaning all cells but sperm and egg. What would the mitosi ...
Sequence 1 - Human DNA
Sequence 1 - Human DNA

... 6. Another mutation changes the insulin gene to read T C T (instead of the normal T A G). Will this person be diabetic? Explain. ...
BIOTECHNOLOGY
BIOTECHNOLOGY

... DNA fingerprinting and cloning that uses biological systems and living organisms to make or modify products or processes for specific use. ...
pbs weekly syllabus - Madison Local Schools
pbs weekly syllabus - Madison Local Schools

... PBS WEEKLY SYLLABUS WEEK OF 2/10 – 2/14 CONCEPTS WE’LL BE LEARNING THIS WEEK: ...
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... to the homeorrhetic (Waddington) rules of the networks themselves. The final step, from metabolism to phenotypes is, in turn, strongly affected by exterior signals transferred to genes through the cytoplasm, activating, inhibiting and modulating response-buffering batteries of genes specific to diff ...
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Organization of Eukaryotic DNA Dr: Hussein abdelaziz

Name: DNA Stations Once Mendel`s work was rediscovered in the
Name: DNA Stations Once Mendel`s work was rediscovered in the

... Station 3--Hershey and Chase Watch the video and answer the questions as you go. You may need to watch it more than once. A little background info: Bacteriophages are viruses that infect bacteria. Although they are not living, they do contain DNA. At the time no one knew whether the genetic material ...
Visualizing DNA
Visualizing DNA

... is  constructed  by  using  gel  electrophoresis  to   separate  the  DNA  fragments  from  several  of   ...
DNA made Simple
DNA made Simple

... Scale analogy - If every cell in your hand was the size of a grain of sand, your hand would be the size of a school bus! Cells – they are unique Each cell has its own job. Some cells help us detect light and see, some cells help us hear, other cells help us digest food by secreting enzymes. There ar ...
DNA Extraction - Sterlingmontessoriscience
DNA Extraction - Sterlingmontessoriscience

... other small glass containers, each about 1/3 full. Add a pinch of enzymes to each test tube and stir gently. Be careful! If you stir too hard, you'll break up the DNA, making it harder to see. ...
Bio 181 Weekly Internet
Bio 181 Weekly Internet

... Recombinase-based cloning technologies are becoming increasingly popular because of their easy use and high efficiency. These tools exploit bacterial or viral site-specific recombinases like the bacteriophage P1 Cre, the Saccharomyces cerevisiae FLP or the bacteriophage lambda integrase. These enzym ...
Honors Biology Final Exam Review Mrs. Speirs Directions: In no
Honors Biology Final Exam Review Mrs. Speirs Directions: In no

... Antiparallel Phosphate Deoxyribose Base pairing rules DNA polymerase Mutation What could happen if one letter ATCG in the DNA molecule is changed, duplicated, or deleted? Compare and contrast DNA with RNA in terms of structure, function and location. The three forms of RNA and their functions in the ...
Molecular genetics (cloning)
Molecular genetics (cloning)

... E. Börje Lindström ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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