Example - Hivebench
... the Ta is too close to the Tm, or unless they are unusually long. The optimum length of a primer depends upon its (A+T) content, and the Tm of its partner if one runs the risk of having problems such as described above. Apart from the Tm, a prime consideration is that the primers should be complex e ...
... the Ta is too close to the Tm, or unless they are unusually long. The optimum length of a primer depends upon its (A+T) content, and the Tm of its partner if one runs the risk of having problems such as described above. Apart from the Tm, a prime consideration is that the primers should be complex e ...
The molecular epidemiology of iridovirus in Murray cod
... Iridoviruses have emerged over 20 years to cause epizootics in finfish and amphibians in many countries. They may have originated in tropical Asia and spread through trade in farmed food fish or ornamental fish, but this has been difficult to prove. Consequently, MCP, ATPase and other viral genes we ...
... Iridoviruses have emerged over 20 years to cause epizootics in finfish and amphibians in many countries. They may have originated in tropical Asia and spread through trade in farmed food fish or ornamental fish, but this has been difficult to prove. Consequently, MCP, ATPase and other viral genes we ...
Teacher Guide - Science Take-Out
... Every effort has been made to reduce the use of hazardous chemicals in Science Take-Out kits. Most kits contain common household chemicals or chemicals that pose little or no risk. ...
... Every effort has been made to reduce the use of hazardous chemicals in Science Take-Out kits. Most kits contain common household chemicals or chemicals that pose little or no risk. ...
Creating conditional dual fluorescence labelled transgenic animals
... critical to the field. We developed a fast and straightforward method to determine ncRNAtransgene copy number, orientation, and insertion site in the genome. Furthermore, upon tissuespecific expression of ncRNA, a Cre-loxP mediated dual-fluorescence expression system facilitates fluorescence signal ...
... critical to the field. We developed a fast and straightforward method to determine ncRNAtransgene copy number, orientation, and insertion site in the genome. Furthermore, upon tissuespecific expression of ncRNA, a Cre-loxP mediated dual-fluorescence expression system facilitates fluorescence signal ...
Gene as the unit of genetic material - E
... The part of the cell which occurs between the plasma membrane and nuclear envelope is known as the cytoplasm. It forms most essential part of the cell because it is seat of all biosynthetic and bio energetic functions. Most of the phenotypic characters are controlled by the genes present in the chro ...
... The part of the cell which occurs between the plasma membrane and nuclear envelope is known as the cytoplasm. It forms most essential part of the cell because it is seat of all biosynthetic and bio energetic functions. Most of the phenotypic characters are controlled by the genes present in the chro ...
Viruses & Microorganisms
... lysogenic cycle are unusual. 2. They contain RNA, but their host cell’s genetic material is DNA. ...
... lysogenic cycle are unusual. 2. They contain RNA, but their host cell’s genetic material is DNA. ...
Cloning, characterization and in vitro and in planta expression of a
... b(1,6)glucan elicitors that are released during this process are perceived by the plant, by a presumed plasma membrane-bound receptor, and a signaling cascade is initiated, resulting in the up-regulation of a number of plant defenses. Since the recent identification of GIPs, studies have focused on ...
... b(1,6)glucan elicitors that are released during this process are perceived by the plant, by a presumed plasma membrane-bound receptor, and a signaling cascade is initiated, resulting in the up-regulation of a number of plant defenses. Since the recent identification of GIPs, studies have focused on ...
Lecture 10 - University of New England
... • This line gives the width (‘width’), number of occurrences in the training set (‘sites’), log likelihood ratio (‘llr’) and E-value of the motif. Each motif describes a pattern of a fixed width and no gaps are allowed in MEME motifs. MEME numbers the motifs consecutively from one as it finds them. ...
... • This line gives the width (‘width’), number of occurrences in the training set (‘sites’), log likelihood ratio (‘llr’) and E-value of the motif. Each motif describes a pattern of a fixed width and no gaps are allowed in MEME motifs. MEME numbers the motifs consecutively from one as it finds them. ...
PPT1
... • Collect all known sequences that bind a certain TF. • Align all sequences (using multiple sequence alignment). • Compute the frequency of each nucleotide in each position (PSPM). • Incorporate background frequency for each nucleotide (PSSM). ...
... • Collect all known sequences that bind a certain TF. • Align all sequences (using multiple sequence alignment). • Compute the frequency of each nucleotide in each position (PSPM). • Incorporate background frequency for each nucleotide (PSSM). ...
Foundations in Microbiology
... (a) DNA heating and cooling. DNA responds to heat by denaturing—losing nucleotides to hydrogen its hydrogen bonding and thereby separating into its two strands. When cooled, the two strands rejoin at complementary regions. The two strands bond and the DNA will need not be from the same organisms as ...
... (a) DNA heating and cooling. DNA responds to heat by denaturing—losing nucleotides to hydrogen its hydrogen bonding and thereby separating into its two strands. When cooled, the two strands rejoin at complementary regions. The two strands bond and the DNA will need not be from the same organisms as ...
Ho - Engineering Computing Facility
... cells modify their gene expression patterns in order to survive and thrive. Consequently, profiling the transcriptome (mRNA) indicates how cells react to better their own chances at survival, or to protect surrounding tissues. Although several methods exist to measure gene expression, serial analysi ...
... cells modify their gene expression patterns in order to survive and thrive. Consequently, profiling the transcriptome (mRNA) indicates how cells react to better their own chances at survival, or to protect surrounding tissues. Although several methods exist to measure gene expression, serial analysi ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
... An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low levels of mutagenicity and toxicity compared ...
... An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low levels of mutagenicity and toxicity compared ...
Bacteria-based communication in nanonetworks
... bacteria sense, via chemoreceptors, the concentration gradient of specific particles and swim towards higher concentrations of beneficial particles called attractants. E. coli can sense at least 12 different attractants using different chemoreceptors that cause independent chemotactic responses [3]. ...
... bacteria sense, via chemoreceptors, the concentration gradient of specific particles and swim towards higher concentrations of beneficial particles called attractants. E. coli can sense at least 12 different attractants using different chemoreceptors that cause independent chemotactic responses [3]. ...
Chromatin regulates origin activity in Drosophila follicle cells
... identity can change during development, suggesting that there are epigenetic influences. A notable example of developmental specificity occurs in Drosophila, where somatic follicle cells of the ovary transition from genomic replication to exclusive rereplication at origins that control amplification ...
... identity can change during development, suggesting that there are epigenetic influences. A notable example of developmental specificity occurs in Drosophila, where somatic follicle cells of the ovary transition from genomic replication to exclusive rereplication at origins that control amplification ...
Chemical Genetics
... This experiment was the beginning of a series of investigations on the relationship between genetics and metabolism, partly because it was now possible to produce mutants of the desired biochemical type instead of waiting for them to occur by chance. Over the years the efficiency with which mutants ...
... This experiment was the beginning of a series of investigations on the relationship between genetics and metabolism, partly because it was now possible to produce mutants of the desired biochemical type instead of waiting for them to occur by chance. Over the years the efficiency with which mutants ...
DNA methylation involved in proline accumulation in - Funpec-RP
... (Siripornadulsil et al., 2002; Verbruggen and Hermans, 2008). Recent findings have suggested that proline may also play a role in flowering and development both as a metabolite and a signal molecule (Mattioli et al., 2009). In any case, proline clearly plays crucial roles not only in drought toleran ...
... (Siripornadulsil et al., 2002; Verbruggen and Hermans, 2008). Recent findings have suggested that proline may also play a role in flowering and development both as a metabolite and a signal molecule (Mattioli et al., 2009). In any case, proline clearly plays crucial roles not only in drought toleran ...
De Bruijn Graphs for DNA Sequencing (Part 1)
... that are complementary to substrings of length k of the fragment. Modelli Biologici Discreti ...
... that are complementary to substrings of length k of the fragment. Modelli Biologici Discreti ...
CpG Mutation Rates in the Human Genome Are
... We chose to discard repetitive sequences because it would be more difficult operationally to establish their orthology with the same level of confidence. Designing such a computer program would require not only performing an additional series of sequence comparisons to establish the sequence context ...
... We chose to discard repetitive sequences because it would be more difficult operationally to establish their orthology with the same level of confidence. Designing such a computer program would require not only performing an additional series of sequence comparisons to establish the sequence context ...
Influence of industrial contamination on mobile genetic elements
... Yet, how MGE abundance is affected by environmental conditions and selective pressures is poorly understood and remains to be tested systematically. In this study, we examined the potential contribution of integrons to bacterial evolution under varying degrees of selective pressure from metal exposu ...
... Yet, how MGE abundance is affected by environmental conditions and selective pressures is poorly understood and remains to be tested systematically. In this study, we examined the potential contribution of integrons to bacterial evolution under varying degrees of selective pressure from metal exposu ...
Identification of C. elegans lin
... protein coding sequences of the lin-4 rescuing region from the four species. These four lin4 genes contain no conserved protein sequence that begins and ends with conventional start and stop codons or that can be predicted to be assembled using conventional C. elegans splice donor and acceptor sites ...
... protein coding sequences of the lin-4 rescuing region from the four species. These four lin4 genes contain no conserved protein sequence that begins and ends with conventional start and stop codons or that can be predicted to be assembled using conventional C. elegans splice donor and acceptor sites ...
Supplemental Digital Content
... extension. As positive control and reference an in-house cyclophilin (gCYC) qPCR was included. The specificities of the in house KRAS mutation assays varied between 0.1% and better than 0.01%. The final primer mixtures are shown in Table 1. All qPCR reactions were performed in a volume of 25 µl in ...
... extension. As positive control and reference an in-house cyclophilin (gCYC) qPCR was included. The specificities of the in house KRAS mutation assays varied between 0.1% and better than 0.01%. The final primer mixtures are shown in Table 1. All qPCR reactions were performed in a volume of 25 µl in ...
Trawling DNA Databases For Partial Matches: What Is The FBI
... resolve the dispute by the mid-1990s. Since 2000, however, reports have surfaced of shocking numbers of “partial matches” among samples within large DNA databases, and some scientists have complained that the infinitesimal figures used in court to estimate the probability of a random match are no be ...
... resolve the dispute by the mid-1990s. Since 2000, however, reports have surfaced of shocking numbers of “partial matches” among samples within large DNA databases, and some scientists have complained that the infinitesimal figures used in court to estimate the probability of a random match are no be ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.