Reg Bio DNA tech 2013 ppt
... Manipulation and alteration of genes for practical purposes (use DNA technology) - identify genes for specific traits - transfer genes from one organism to another ...
... Manipulation and alteration of genes for practical purposes (use DNA technology) - identify genes for specific traits - transfer genes from one organism to another ...
Grimmer presentation
... Supported by the Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior Interior Business Center (DoI/ICB) contract number D15PC0002. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation ...
... Supported by the Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior Interior Business Center (DoI/ICB) contract number D15PC0002. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation ...
7th Grade Science-Chapter 11 Test Study Guide: Human Genetics
... Two techniques for selective breeding are: inbreeding and hybridization. Inbreeding- breeding technique that involves crossing two individuals that have similar desirable characteristics. This process produces organisms that are genetically very similar. This type of breeding leads to a greater cha ...
... Two techniques for selective breeding are: inbreeding and hybridization. Inbreeding- breeding technique that involves crossing two individuals that have similar desirable characteristics. This process produces organisms that are genetically very similar. This type of breeding leads to a greater cha ...
CP Biology Second Semester Final Exam Review Guide
... 3. What is a scientific theory? 4. Describe the Galapagos Islands (why is life there so diverse?) 5. Why were Darwin’s ideas so controversial at the time? 6. What did James Hutton propose? 7. What did Charles Lyell propose? 8. How did the above scientists help shape Darwin’s theory? 9. Describe AND ...
... 3. What is a scientific theory? 4. Describe the Galapagos Islands (why is life there so diverse?) 5. Why were Darwin’s ideas so controversial at the time? 6. What did James Hutton propose? 7. What did Charles Lyell propose? 8. How did the above scientists help shape Darwin’s theory? 9. Describe AND ...
Y12 Biology Year 1 AS LOs Student Teacher 1
... In prokaryotic cells, DNA molecules are short, circular and not associated with proteins. In the nucleus of eukaryotic cells, DNA molecules are very long, linear and associated with proteins, called histones. Together a DNA molecule and its associated proteins form a chromosome. The mitochondria and ...
... In prokaryotic cells, DNA molecules are short, circular and not associated with proteins. In the nucleus of eukaryotic cells, DNA molecules are very long, linear and associated with proteins, called histones. Together a DNA molecule and its associated proteins form a chromosome. The mitochondria and ...
Concept Check Questions with answers
... Some human genes are too large to be incorporated into bacterial plasmids. Bacterial cells lack the means to process RNA transcripts, and even if the need for RNA processing is avoided by using cDNA, bacteria lack enzymes to catalyze the post-translational processing that many human proteins undergo ...
... Some human genes are too large to be incorporated into bacterial plasmids. Bacterial cells lack the means to process RNA transcripts, and even if the need for RNA processing is avoided by using cDNA, bacteria lack enzymes to catalyze the post-translational processing that many human proteins undergo ...
Note 8.1 - Cloning DNA
... Restriction enzymes were first discovered by DR. Hamilton Smith in 1970, as he studied bacteria and their ability to resisted viral infections. Since the start of his research there have been 2500 restriction enzymes classified with a 200 target sequences. The primary functions of restriction enzyme ...
... Restriction enzymes were first discovered by DR. Hamilton Smith in 1970, as he studied bacteria and their ability to resisted viral infections. Since the start of his research there have been 2500 restriction enzymes classified with a 200 target sequences. The primary functions of restriction enzyme ...
Nucleotides, nucleic acids and the genetic material It all started with
... • 2. Helicase accomplishes unwinding of the original double strand, once supercoiling has been eliminated by the topoisomerase. The two strands very much want to bind together because of their hydrogen bonding affinity for each other, so the helicase activity requires energy ...
... • 2. Helicase accomplishes unwinding of the original double strand, once supercoiling has been eliminated by the topoisomerase. The two strands very much want to bind together because of their hydrogen bonding affinity for each other, so the helicase activity requires energy ...
What is Biotechnology?
... • Once the location of the DNA sequence has been located, scientists can use restrictiion enzymes to separate the DNA at a particular location on the gene • Once the pieces of DNA are removed other DNA canbe spliced in or recombined with the remaining DNA – This results in recombinant DNA ...
... • Once the location of the DNA sequence has been located, scientists can use restrictiion enzymes to separate the DNA at a particular location on the gene • Once the pieces of DNA are removed other DNA canbe spliced in or recombined with the remaining DNA – This results in recombinant DNA ...
Nucleotides, nucleic acids and the genetic material
... DNA. The tension holding the helix in its coiled and supercoiled structure can be broken by nicking a single strand of DNA. Try this with string. Twist two strings together, holding both the top and the bottom. If you cut only one of the two strings, the tension of the twisting is released and the s ...
... DNA. The tension holding the helix in its coiled and supercoiled structure can be broken by nicking a single strand of DNA. Try this with string. Twist two strings together, holding both the top and the bottom. If you cut only one of the two strings, the tension of the twisting is released and the s ...
Slide 1
... Describe the characteristics of plasmids Explain how plasmids are used in cloning a gene Describe the function of restriction enzymes Explain how to use restriction enzymes to create a recombinant plasmid ...
... Describe the characteristics of plasmids Explain how plasmids are used in cloning a gene Describe the function of restriction enzymes Explain how to use restriction enzymes to create a recombinant plasmid ...
Nucleic Acids and Nucleotides
... DNA is the genetic material found in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is found in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the DNA is not enclosed in a membranous envelope. The entire geneti ...
... DNA is the genetic material found in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is found in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the DNA is not enclosed in a membranous envelope. The entire geneti ...
DNA Isolation for Low-Melting Point Agarose (using elu
... Load DNA sample onto the column slowly (1-2 drops/second). NOTE: When recovering DNA from low-melt temperature agarose, use of the pre-filter is not recommended. Consult the protocols booklet for specific parameters of different types of nucleic acid purification (i.e. DNA purification when LMP agar ...
... Load DNA sample onto the column slowly (1-2 drops/second). NOTE: When recovering DNA from low-melt temperature agarose, use of the pre-filter is not recommended. Consult the protocols booklet for specific parameters of different types of nucleic acid purification (i.e. DNA purification when LMP agar ...
Paper Plasmid activity - Liberty Union High School District
... 4. The start and stop sequences for transcribing the Jellyfish GFP or Glo gene are highlighted. 5. These are needed to transcribe the gene properly when it is read. 6. The HindIII & EcoR1 restriction enzyme cutting sites (sequences of bases) are marked in bold on the Jellyfish Glo gene DNA. 7. The t ...
... 4. The start and stop sequences for transcribing the Jellyfish GFP or Glo gene are highlighted. 5. These are needed to transcribe the gene properly when it is read. 6. The HindIII & EcoR1 restriction enzyme cutting sites (sequences of bases) are marked in bold on the Jellyfish Glo gene DNA. 7. The t ...
Cell Review - Oakland Schools Online Studies
... •Protein kinases, give the go-ahead signals at the G1 and G2 checkpoints ...
... •Protein kinases, give the go-ahead signals at the G1 and G2 checkpoints ...
Mitosis Lecture
... a. involves the division of somatic (body) cells b. genetic material must be duplicated so each new cell can have a full set of instructions 2. Meiosis a. involves the division of germ cells (gametes - sperm & egg) b. genetic material must be reduced from a full set (diploid) to a half set (haploid) ...
... a. involves the division of somatic (body) cells b. genetic material must be duplicated so each new cell can have a full set of instructions 2. Meiosis a. involves the division of germ cells (gametes - sperm & egg) b. genetic material must be reduced from a full set (diploid) to a half set (haploid) ...
Outline for the Second Part of the Bio Final
... Function of mRNA, tRNA, and rRNA Know how to find the complementary DNA strand Know how to code mRNA from DNA Know how to code for amino acids Types of Mutations o Inversion, Deletion, Insertion, Duplication, Translocation ...
... Function of mRNA, tRNA, and rRNA Know how to find the complementary DNA strand Know how to code mRNA from DNA Know how to code for amino acids Types of Mutations o Inversion, Deletion, Insertion, Duplication, Translocation ...
Unit VII Study Guide KEY
... _repressible____ operon, on the other hand, is normally on. Therefore, its repressor is synthesized in a _dysfunctional_______ form. An example is the _trp____ operon. When _tryptophan_____ is present in the cell due to over-production or availability from surrounding environment, it binds to the re ...
... _repressible____ operon, on the other hand, is normally on. Therefore, its repressor is synthesized in a _dysfunctional_______ form. An example is the _trp____ operon. When _tryptophan_____ is present in the cell due to over-production or availability from surrounding environment, it binds to the re ...
Nucleic acids
... • The two strands are Antiparallel wrt 5’& 3’ ends. • They are held together by Hydrogen Bonds between the bases. • H-Bond energies are weak BUT there are many of them which makes the duplex DNA very stable. • Bases are Complementary such that: – A always pairs with T (2 H Bonds). – C always pairs w ...
... • The two strands are Antiparallel wrt 5’& 3’ ends. • They are held together by Hydrogen Bonds between the bases. • H-Bond energies are weak BUT there are many of them which makes the duplex DNA very stable. • Bases are Complementary such that: – A always pairs with T (2 H Bonds). – C always pairs w ...
Gene Cloning
... using mRNA as a template. This process also requires a primer and an enzyme, reverse transcriptase (a DNA polymerase that synthesizes a DNA strand from the mRNA) • This complementary DNA is called cDNA • cDNA may be attached to a vector such as a plasmid and then introduced into bacterial cells. ...
... using mRNA as a template. This process also requires a primer and an enzyme, reverse transcriptase (a DNA polymerase that synthesizes a DNA strand from the mRNA) • This complementary DNA is called cDNA • cDNA may be attached to a vector such as a plasmid and then introduced into bacterial cells. ...
Biology-Chapter8 (Biology
... code and make their proteins. B. DNA is in the nucleus because the nucleus also stores amino acids to make the proteins in the directions. C. The chromosomes where the DNA code is stored are much too large to be read by individual ribosomes, so many RNA messages are sent from the nucleus. D. The DNA ...
... code and make their proteins. B. DNA is in the nucleus because the nucleus also stores amino acids to make the proteins in the directions. C. The chromosomes where the DNA code is stored are much too large to be read by individual ribosomes, so many RNA messages are sent from the nucleus. D. The DNA ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.