Defective HIV-1 Proviruses Can Be Transcribed Upon Activation
... suppressive antiretroviral therapy were activated with anti-CD3/CD28 costimulation under enfuvirtide to prevent new rounds of in vitro infection. To examine whether cells containing intact or defective HIV-1 can be eliminated by CTLs, peripheral blood mononuclear cells (PBMC) containing autologous C ...
... suppressive antiretroviral therapy were activated with anti-CD3/CD28 costimulation under enfuvirtide to prevent new rounds of in vitro infection. To examine whether cells containing intact or defective HIV-1 can be eliminated by CTLs, peripheral blood mononuclear cells (PBMC) containing autologous C ...
Full Text - BioTechniques
... pensing performance equal to those achieved when spotting nucleic acids or organic compounds (such as fluorescein) was observed. DISCUSSION The Tango and Hydra systems incorporate nondisposable precision glass syringes that offer a new type of printing technology free of disposable tips, pins, or in ...
... pensing performance equal to those achieved when spotting nucleic acids or organic compounds (such as fluorescein) was observed. DISCUSSION The Tango and Hydra systems incorporate nondisposable precision glass syringes that offer a new type of printing technology free of disposable tips, pins, or in ...
File
... Concept 3 – Genetics Learning Concept Investigate the transmission of characteristics from parents to offspring, and identify examples of characteristics in offspring that are: The same as the characteristics of both parents The same as the characteristics of one parent Intermediate between ...
... Concept 3 – Genetics Learning Concept Investigate the transmission of characteristics from parents to offspring, and identify examples of characteristics in offspring that are: The same as the characteristics of both parents The same as the characteristics of one parent Intermediate between ...
A bacterial two-hybrid genome fragment library for
... Bacterial gene regulation is controlled by complex regulatory cascades which integrate input environmental signals and adapt specific and adequate output cellular responses. These complex networks are far from being elucidated, in particular in Pseudomonas aeruginosa. In the present study, we develo ...
... Bacterial gene regulation is controlled by complex regulatory cascades which integrate input environmental signals and adapt specific and adequate output cellular responses. These complex networks are far from being elucidated, in particular in Pseudomonas aeruginosa. In the present study, we develo ...
S1 Document.
... QuikChange site-directed mutagenesis kit (Agilent Technologies). Primers used for mutagenesis are listed in Table S1. ...
... QuikChange site-directed mutagenesis kit (Agilent Technologies). Primers used for mutagenesis are listed in Table S1. ...
Document
... individuals with the disease are selected in the population, a matching group of controls without disease are then chosen, and the genotypes of individuals in the two groups are determined; an association between disease and genotype is then calculated by an odds ratio. Odds are ratios. With use of ...
... individuals with the disease are selected in the population, a matching group of controls without disease are then chosen, and the genotypes of individuals in the two groups are determined; an association between disease and genotype is then calculated by an odds ratio. Odds are ratios. With use of ...
The global repressor FliZ antagonizes gene
... FliZ binding sites correspond to known pS-dependent promoters, promoter activity is not a prerequisite for FliZ binding and repressor function. Thus, we demonstrate that FliZ also feedback-controls flagellar gene expression by binding to a site in the flhDC control region that shows similarity only ...
... FliZ binding sites correspond to known pS-dependent promoters, promoter activity is not a prerequisite for FliZ binding and repressor function. Thus, we demonstrate that FliZ also feedback-controls flagellar gene expression by binding to a site in the flhDC control region that shows similarity only ...
Green Fluorescent Protein (GFP) Purification Kit
... Is Finding a Needle in a Hay Stack Easier When it Glows? This laboratory activity is designed to follow Biotechnology Explorer Kit 1, “Bacterial Transformation—the pGLO System” (catalog number 166-0003-EDU). Students begin this activity with the bacteria they genetically transformed using the plasm ...
... Is Finding a Needle in a Hay Stack Easier When it Glows? This laboratory activity is designed to follow Biotechnology Explorer Kit 1, “Bacterial Transformation—the pGLO System” (catalog number 166-0003-EDU). Students begin this activity with the bacteria they genetically transformed using the plasm ...
Journal of Molecular Biology
... only cuts once, 2.7 kb from one end (Rothstein & Reznikoff, 1981). The sizes of the Hind111 and BarnHI restrict’ion fragments of each derivative were used to locate each insertion precisely. The results, presented in Figure 3. show that the presumed insertions in phoR as well as those in phoB are cl ...
... only cuts once, 2.7 kb from one end (Rothstein & Reznikoff, 1981). The sizes of the Hind111 and BarnHI restrict’ion fragments of each derivative were used to locate each insertion precisely. The results, presented in Figure 3. show that the presumed insertions in phoR as well as those in phoB are cl ...
What Do You Mean, “Epigenetic”?
... Definitions of Epigenetics It was largely through a shared interest in development and cellular differentiation that Waddington, Nanney, and others came to use the term epigenetic; however, the focus of those within the field did vary, with some, such as Waddington, being more concerned with gene regu ...
... Definitions of Epigenetics It was largely through a shared interest in development and cellular differentiation that Waddington, Nanney, and others came to use the term epigenetic; however, the focus of those within the field did vary, with some, such as Waddington, being more concerned with gene regu ...
disease revealed by a metagenomic approach Reduced diversity of
... inserts having similar end sequences (data not shown), confirmed that major biases can be avoided by using this method. The DNA macroarrays were constructed after fosmid DNA extraction from the 50 000 recombinant clones and spotting on two nylon membranes, one for HSL and one for CPL (fig 1). 16S rR ...
... inserts having similar end sequences (data not shown), confirmed that major biases can be avoided by using this method. The DNA macroarrays were constructed after fosmid DNA extraction from the 50 000 recombinant clones and spotting on two nylon membranes, one for HSL and one for CPL (fig 1). 16S rR ...
enzymes - 11biolcsc
... The substrate (S) binds to the active site of the enzyme (E) to form an enzyme-substrate complex (ES) ...
... The substrate (S) binds to the active site of the enzyme (E) to form an enzyme-substrate complex (ES) ...
5 - Parkway C-2
... • A cell translates an mRNA message into protein with the help of transfer RNA (tRNA) • Molecules of tRNA are not identical: – Each carries a specific amino acid on one end – Each has an anticodon on the other end; the anticodon base-pairs with a complementary codon on mRNA ...
... • A cell translates an mRNA message into protein with the help of transfer RNA (tRNA) • Molecules of tRNA are not identical: – Each carries a specific amino acid on one end – Each has an anticodon on the other end; the anticodon base-pairs with a complementary codon on mRNA ...
Translation
... RNA polymerase adds to the first transcribed DNA nucleotide complementary RNA nucleotide and thus the transcription is started. Elongation phase of transcription: RNA polymerase continues along template DNA strand, it unwinds ahead a short segment of DNA double helix and at the same time it synthesi ...
... RNA polymerase adds to the first transcribed DNA nucleotide complementary RNA nucleotide and thus the transcription is started. Elongation phase of transcription: RNA polymerase continues along template DNA strand, it unwinds ahead a short segment of DNA double helix and at the same time it synthesi ...
Translation
... RNA polymerase adds to the first transcribed DNA nucleotide complementary RNA nucleotide and thus the transcription is started. Elongation phase of transcription: RNA polymerase continues along template DNA strand, it unwinds ahead a short segment of DNA double helix and at the same time it synthesi ...
... RNA polymerase adds to the first transcribed DNA nucleotide complementary RNA nucleotide and thus the transcription is started. Elongation phase of transcription: RNA polymerase continues along template DNA strand, it unwinds ahead a short segment of DNA double helix and at the same time it synthesi ...
Translation
... RNA polymerase adds to the first transcribed DNA nucleotide complementary RNA nucleotide and thus the transcription is started. Elongation phase of transcription: RNA polymerase continues along template DNA strand, it unwinds ahead a short segment of DNA double helix and at the same time it synthesi ...
... RNA polymerase adds to the first transcribed DNA nucleotide complementary RNA nucleotide and thus the transcription is started. Elongation phase of transcription: RNA polymerase continues along template DNA strand, it unwinds ahead a short segment of DNA double helix and at the same time it synthesi ...
UV-Targeted Dinucleotides Are Not Depleted in Light
... et al. 2003; Rocap et al. 2003) and, therefore, exposed to different intensities of UV radiation. This seemed to make it an ideal model organism for investigating this hypothesis. Dufresne et al. (2003) have shown that the SS120 strain is adapted to living at a depth of 120 m. The MIT 9313 strain is ...
... et al. 2003; Rocap et al. 2003) and, therefore, exposed to different intensities of UV radiation. This seemed to make it an ideal model organism for investigating this hypothesis. Dufresne et al. (2003) have shown that the SS120 strain is adapted to living at a depth of 120 m. The MIT 9313 strain is ...
PDF
... of a focused light beam [12, 13]. Soon thereafter, magnetic tweezers were invented to allow the manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their o ...
... of a focused light beam [12, 13]. Soon thereafter, magnetic tweezers were invented to allow the manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their o ...
Hyper-eccentric structural genes in the mitochondrial genome of the
... Diplonemid mitochondria are considered to have very eccentric structural genes. Coding regions of individual diplonemid mitochondrial genes are fragmented into small pieces and found on different circular DNAs. Short RNAs transcribed from each DNA molecule mature through a unique RNA maturation proc ...
... Diplonemid mitochondria are considered to have very eccentric structural genes. Coding regions of individual diplonemid mitochondrial genes are fragmented into small pieces and found on different circular DNAs. Short RNAs transcribed from each DNA molecule mature through a unique RNA maturation proc ...
Molecular cloning and biochemical characterization of a
... converted to volatile aldehydes, alcohols and ketones through the action of other enzymes of the lipoxygenase pathway, e.g. hydroperoxide lyases and isomerases [1]. Some of these volatile compounds are thought to induce the synthesis of molecules, such as systemin, that are important in intercellula ...
... converted to volatile aldehydes, alcohols and ketones through the action of other enzymes of the lipoxygenase pathway, e.g. hydroperoxide lyases and isomerases [1]. Some of these volatile compounds are thought to induce the synthesis of molecules, such as systemin, that are important in intercellula ...
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof
... for 2AP quenching in dsDNA is still controversial,18−20 it is widely accepted that stacking interactions between 2AP and neighboring bases result in fluorescence quenching.16,21,22 To characterize the fluorescence of 2AP at the singlemolecule level, we surface-immobilized dsDNA on a quartz microscope ...
... for 2AP quenching in dsDNA is still controversial,18−20 it is widely accepted that stacking interactions between 2AP and neighboring bases result in fluorescence quenching.16,21,22 To characterize the fluorescence of 2AP at the singlemolecule level, we surface-immobilized dsDNA on a quartz microscope ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.