... The moistened filters were placed on agar plates of the same medium that had been overlayed with l-2 ml of liquid medium. Droplets of conidial suspension from each strain to be tested were then spotted on the filters. The inoculated filters were incubated for 24 h at 30° C. The filters were then air ...
Chapter 7: Microbial Genetics 10/8/2015
... The lac repressor is bound by lactose and inactive, and the low glucose levels activate CAP, a transcriptional activator, which binds the CAP site & enhances binding of RNA polymerase to the promoter. Since lactose is a much more important source of energy in the absence of glucose, the lac operon i ...
... The lac repressor is bound by lactose and inactive, and the low glucose levels activate CAP, a transcriptional activator, which binds the CAP site & enhances binding of RNA polymerase to the promoter. Since lactose is a much more important source of energy in the absence of glucose, the lac operon i ...
Co-dominance • WT protein will make WT phenotype. Mutant gene
... • Repression of genes involved in the lytic cycle. Bound c1 repressor blocks expression of genes either side of it on the λ chromosome, thus blocking the lytic cycle (stops expression of genes to make more phage & lyse cell). It is now undergoing the lysogenic pathway. • Production of integrase ...
... • Repression of genes involved in the lytic cycle. Bound c1 repressor blocks expression of genes either side of it on the λ chromosome, thus blocking the lytic cycle (stops expression of genes to make more phage & lyse cell). It is now undergoing the lysogenic pathway. • Production of integrase ...
BIO 10 Lecture 9 REPRODUCTION: MITOSIS AND MEIOSIS
... • Must have a way of reducing the chromosome number by half, to get 23(n) + 23(n) = 46 (2n) total for the new organism each generation – Cells with half the number of chromosomes (one of each) are haploid and are called gametes (sperm and eggs) – These cells are made only in sexually reproducing or ...
... • Must have a way of reducing the chromosome number by half, to get 23(n) + 23(n) = 46 (2n) total for the new organism each generation – Cells with half the number of chromosomes (one of each) are haploid and are called gametes (sperm and eggs) – These cells are made only in sexually reproducing or ...
41. Situations in which one allele for a gene is not
... determine whether a particular allele of a gene is dominant or recessive. c. identify similarities and differences in the genomes of different kinds of organisms. d. compare the phenotypes of different organisms. A Punnett square shows all of the following EXCEPT a. the genotypes of the offspring. b ...
... determine whether a particular allele of a gene is dominant or recessive. c. identify similarities and differences in the genomes of different kinds of organisms. d. compare the phenotypes of different organisms. A Punnett square shows all of the following EXCEPT a. the genotypes of the offspring. b ...
Bio08 DNA RNA
... • The ribosome will continue to add new amino acids until a stop signal is reached on the mRNA molecule. • The stop codon can be either UAA, UAG, or ...
... • The ribosome will continue to add new amino acids until a stop signal is reached on the mRNA molecule. • The stop codon can be either UAA, UAG, or ...
Genetics Science Learning Worksheet How Does DNA Determine
... How Does DNA Determine the Traits of an Organism Introduction: In this simulation, you will examine the DNA sequence of a fictitious organism - the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to a ...
... How Does DNA Determine the Traits of an Organism Introduction: In this simulation, you will examine the DNA sequence of a fictitious organism - the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to a ...
Biochem17_DNA_RNA
... • The ribosome will continue to add new amino acids until a stop signal is reached on the mRNA molecule. • The stop codon can be either UAA, UAG, or ...
... • The ribosome will continue to add new amino acids until a stop signal is reached on the mRNA molecule. • The stop codon can be either UAA, UAG, or ...
2657/113 Recombinant DNA……To Exempt or Non
... What is recombinant DNA (rDNA) you may be asking yourself? The National Institutes of Health Office of Biotechnology Activities (NIH-OBA) defines rDNA molecules as either: (i) molecules that are constructed outside living cells by joining natural or synthetic DNA segments to DNA molecules that can r ...
... What is recombinant DNA (rDNA) you may be asking yourself? The National Institutes of Health Office of Biotechnology Activities (NIH-OBA) defines rDNA molecules as either: (i) molecules that are constructed outside living cells by joining natural or synthetic DNA segments to DNA molecules that can r ...
Nerve activates contraction
... chromosomes (YACs) - an origin site for replication, a centromere, and two telomeres with foreign DNA. ...
... chromosomes (YACs) - an origin site for replication, a centromere, and two telomeres with foreign DNA. ...
NOTES AND PROBLEM SET 3
... 1. Consider a semiflexible polymer of length L and persistence length lp : L can be either larger or smaller than lp . Consider this polymer to be confined within the long tube of diameter D. The tube diameter is much smaller than both L and lp . Estimate the number of collision points between the t ...
... 1. Consider a semiflexible polymer of length L and persistence length lp : L can be either larger or smaller than lp . Consider this polymer to be confined within the long tube of diameter D. The tube diameter is much smaller than both L and lp . Estimate the number of collision points between the t ...
Intro Bioinform 1-19..
... Medical Sciences in their quest for understanding protein structure and function in living organisms. ...
... Medical Sciences in their quest for understanding protein structure and function in living organisms. ...
polymerase chain reaction (pcr)
... Inner self-complementary hairpins of >4 and of dimers >8 should be avoided. •3' terminus is extremely case sensitive - it must not be complementary to any region of the other primer used in the reaction and must provide correct base matching to template. •3’-end Sequence- 3’ position in PCR primers ...
... Inner self-complementary hairpins of >4 and of dimers >8 should be avoided. •3' terminus is extremely case sensitive - it must not be complementary to any region of the other primer used in the reaction and must provide correct base matching to template. •3’-end Sequence- 3’ position in PCR primers ...
Transcription and Translation
... STEP TWO: Each set of three RNA bases is called a codon. For this sequence: AUGCUA, AUG is a codon ...
... STEP TWO: Each set of three RNA bases is called a codon. For this sequence: AUGCUA, AUG is a codon ...
Written Transcript of this video lesson in English (PDF
... organisms, all living things are made of one or more cells, surrounded by a membrane contains the cytoplasm inside in which cell components and organelles swim. The most important ones are the nucleus ...
... organisms, all living things are made of one or more cells, surrounded by a membrane contains the cytoplasm inside in which cell components and organelles swim. The most important ones are the nucleus ...
Isolation and identification of viral DNA from
... included in the total nucleic acid amount. • The amount of DNA decreases as time increases since the lysozyme activity acts longer on the corresponding bacterial targets. • The final yield of the samples are over 30 ng, which is a sufficient amount for future genomic amplifications ...
... included in the total nucleic acid amount. • The amount of DNA decreases as time increases since the lysozyme activity acts longer on the corresponding bacterial targets. • The final yield of the samples are over 30 ng, which is a sufficient amount for future genomic amplifications ...
genetic info notes
... How many chromosomes do human cells have? Then how come you don’t have 92 (46 + 46) chromosomes when you combine an egg with sperm? ...
... How many chromosomes do human cells have? Then how come you don’t have 92 (46 + 46) chromosomes when you combine an egg with sperm? ...
DNA Technology and Genomics I.
... These extensions can form hydrogen-bonded base pairs with complementary single-stranded stretches (sticky ends) on other DNA molecules cut with the same restriction enzyme. ...
... These extensions can form hydrogen-bonded base pairs with complementary single-stranded stretches (sticky ends) on other DNA molecules cut with the same restriction enzyme. ...
Written Transcript of this video lesson in English
... broken down into smaller components. As we all know, complex proteins such as meat and bean are broken down into simple molecules that are more easily absorbed into blood stream and from there to the cell. This is where the nucleic acid DNA starts working. The DNA is responsible for determining the ...
... broken down into smaller components. As we all know, complex proteins such as meat and bean are broken down into simple molecules that are more easily absorbed into blood stream and from there to the cell. This is where the nucleic acid DNA starts working. The DNA is responsible for determining the ...
THE GENETIC PROCESS CHAPTER 4
... The two strands at the replication fork are different in that one strand has an exposed 3' end, and the other an exposed 5' end. For the strand with exposed 3' end, the replication proceeds continuously. The new daughter strand is synthesized in a 5' to 3' direction. This daughter strand is known as ...
... The two strands at the replication fork are different in that one strand has an exposed 3' end, and the other an exposed 5' end. For the strand with exposed 3' end, the replication proceeds continuously. The new daughter strand is synthesized in a 5' to 3' direction. This daughter strand is known as ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.