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DNA Replication
DNA Replication

... a series of proteins aid in the unwinding and separation of the double-stranded DNA molecule. These proteins are required because DNA must be single-stranded before replication can ...
Protein Synthesis
Protein Synthesis

... • Amino Acids are the building blocks for proteins • Since there are 4 nucleotides, when three are grouped together, there are 64 possible triplet combinations (43 = 64) • However, there are only 20 amino acids so some amino acids have more than one codon (ex. GGA, GGC, and GGG all code for glycine) ...
Unit test review
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...  Independent Work  Exit Question ...
Central Dogma of Molecular Biology
Central Dogma of Molecular Biology

... control your characteristics? DNA contains instructions for all the proteins your body makes. Proteins, in turn, determine the structure and function of all your cells. What determines a protein’s structure? It begins with the sequence of amino acids that make up the protein. Instructions for making ...
Application of Microarray- Based Genomic Technology to Mutation
Application of Microarray- Based Genomic Technology to Mutation

... • Single-stranded DNA affect the choice of probe length. • High salt conditions can form internal secondary structures. ...
DNA and PROTEIN SYNTHESIS DNA, functioning as the hereditary
DNA and PROTEIN SYNTHESIS DNA, functioning as the hereditary

... in which DNA affects these traits. DNA functions by coding for the synthesis of proteins. The DNA (deoxyribonucleic acid) is found in the nucleus of the cell, yet protein synthesis occurs outside the nucleus on ribosomes within the cytoplasm. Molecules of RNA (ribonucleic acid) carry a transcribed g ...
Inhibition of Pax 5 activity by expression of its DNA binding domain
Inhibition of Pax 5 activity by expression of its DNA binding domain

... phBSAPls. CAT expression w m determined us described for Fig. I . is obtained if these constructs are co-transfected with phBSAPl s. This confirms the previous observation that the Pax 5 gene product is not sufficient to activate target genes. Co-transfection of the reporter constructs with phBSAP.V ...
Biotechnology 15 ECTS
Biotechnology 15 ECTS

... marked correctly: 1 point. If marked incorrectly: -1 point. If there is no mark: zero point. 1. Without microorganisms, all higher life forms on earth would cease to exist.____ 2. According to our present understanding, each of the major domains has what is known as its own universal ancestor.____ 3 ...
Vincience™ Biofunctionals
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RNA polymerase
RNA polymerase

... DNA that is transcribed into RNA is called a gene). RNA polymerase separates the DNA strands and synthesises a complementary RNA copy from the antisense DNA strand Transcription occurs in a 5’ to 3’ direction: RNA polymerase adds the 5´ end of the free RNA nucleotide to the 3´ end of the growing mRN ...
Genetics Debate - Region 11 Math And Science Teacher Partnership
Genetics Debate - Region 11 Math And Science Teacher Partnership

... 1) Cells are removed from the parent plant, they grow on a special medium-Single cells, plant cells without cell walls and pieces of leaves can often be used to generate a new plant on culture media given the required nutrients and hormones. 2) The gene then transferred into the plant cells by: - el ...
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Microbial Genetics Thesaurus

... amino acid USE amino acids Preferred terms may contain the following information: UF (Use For): The UF reference is used to indicate synonyms or variant forms of a preferred term that are not used in indexing. This reference is also used for those terms that were “upward posted” or indexed under a m ...
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Prometheus & Myriad
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LABORATORY 2: LIGATION OF DNA FRAGMENTS
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... Be sure to include a vector only control: a ligation reaction with no fragment DNA. This will give you an idea of the background ligation (the vector religating without insert). This could be prevented by dephosphorylation of the linearized vector but doesn't always work completely. Ligation of DNA ...
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... a) This is the DNA strand that is complimentary to the DNA strand being transcribed (1) It's sequence will be identical (not complimentary) to the RNA molecule synthesized from the coding strand (a) Except Ts replace Us b) The coding strand sequence is given when talking about double stranded DNA (1 ...
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Chapter13 Section03 cell transformation ppt

... Copyright Pearson Prentice Hall ...
3 Cell Transformation
3 Cell Transformation

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Chapter 19. - Kenston Local Schools
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... often fixed by special enzymes within the cell-but even these are not 100% effective at removing all errors. • Additionally, some errors occur after DNA synthesis has been completed. ...
Translation/Protein Synthesis
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... Made up of repeating units called nucleotides Contains the 5 C sugar ribose. Found in the nucleus & cytoplasm 4 nitrogen bases ...
Crossing-over and Independent Assortment
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... meiosis because of the way that chromosomes line up and then separate into daughter cells. There are two ways genetic recombination occurs, crossing-over and independent assortment. ...
recombinant DNA
recombinant DNA

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7.1 Identification of specific DNA motifs. Note: For this exercise use

Ch17_note_summary
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... what is spliced; this is called alternative RNA splicing. Exon shuffling during cross-over may also be useful in evolution. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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