Protective action of vitamin C against DNA damage induced by
... present work we have clearly shown that the conjugate (NH3)2Pt(SeO3) exerts a pronounced genotoxic effect on normal human peripheral blood lymphocytes. It seems therefore very doubtful whether it is advisable to use intravenously this drug in chemotherapy unless protective steps against its genotoxi ...
... present work we have clearly shown that the conjugate (NH3)2Pt(SeO3) exerts a pronounced genotoxic effect on normal human peripheral blood lymphocytes. It seems therefore very doubtful whether it is advisable to use intravenously this drug in chemotherapy unless protective steps against its genotoxi ...
complex polypeptide-1 gene and related sequences
... Because some of the ^-complex polypeptides had been shown to be abundant in the testes and because the various aspects of the t mutant phenotype described above manifest themselves in the testes, we began a molecular analysis of spermatogenesis. We isolated and started to characterize cDNAs correspo ...
... Because some of the ^-complex polypeptides had been shown to be abundant in the testes and because the various aspects of the t mutant phenotype described above manifest themselves in the testes, we began a molecular analysis of spermatogenesis. We isolated and started to characterize cDNAs correspo ...
Chelatococcus sambhunathii sp. nov., a moderately thermophilic
... synthetic medium defined by Egli et al. (1988). As strain HT4T was isolated from a hot spring, aerobic growth on reduced sulfur compounds was tested in mineral salts medium supplemented with different inorganic sulfur sources (thiosulfate, tetrathionate or elemental sulfur) at a concentration of 0.1 ...
... synthetic medium defined by Egli et al. (1988). As strain HT4T was isolated from a hot spring, aerobic growth on reduced sulfur compounds was tested in mineral salts medium supplemented with different inorganic sulfur sources (thiosulfate, tetrathionate or elemental sulfur) at a concentration of 0.1 ...
Human Genome and Human Genome Project
... Genes are specific sequences of bases that encode instructions on how to make proteins. • Proteins perform most life functions and even make up the majority of cellular structures. Proteins are large, complex molecules made up of smaller subunits called amino acids. A protein folds up into specific ...
... Genes are specific sequences of bases that encode instructions on how to make proteins. • Proteins perform most life functions and even make up the majority of cellular structures. Proteins are large, complex molecules made up of smaller subunits called amino acids. A protein folds up into specific ...
1 CHAPTER 3- DNA FUNCTION – THE EXPRESSION OF GENETIC
... primary structure - amino acid sequence linked by peptide bonds secondary structure - weak bonds (e.g. e.g. H-bonds) between neighbouring amino acids tertiary structure - bonds (e.g. sulfide bonds) between distant amino acids quaternary structure - interactions between different polypeptides to form ...
... primary structure - amino acid sequence linked by peptide bonds secondary structure - weak bonds (e.g. e.g. H-bonds) between neighbouring amino acids tertiary structure - bonds (e.g. sulfide bonds) between distant amino acids quaternary structure - interactions between different polypeptides to form ...
Genetic Engineering and Genomics
... restriction enzyme mixed with the same sequence of DNA always produces the same number of fragments. The length of the pieces may vary if there are variable repeat sequences, for example, but the number of pieces and the places cut are always the same. Before the discovery of restriction enzymes, br ...
... restriction enzyme mixed with the same sequence of DNA always produces the same number of fragments. The length of the pieces may vary if there are variable repeat sequences, for example, but the number of pieces and the places cut are always the same. Before the discovery of restriction enzymes, br ...
Mapping Disease Genes
... diseases. This is often determined only after the genes have been mapped and cloned. Becker muscular dystrophy (OMIM 300376) is caused by partially inactive alleles of the same gene that causes Duschenne muscular dystrophy (OMIM 310200). DMD is more severe, caused by mutations that completely inacti ...
... diseases. This is often determined only after the genes have been mapped and cloned. Becker muscular dystrophy (OMIM 300376) is caused by partially inactive alleles of the same gene that causes Duschenne muscular dystrophy (OMIM 310200). DMD is more severe, caused by mutations that completely inacti ...
History of Biotechnology
... characterizes viruses that infect bacteria, called bacteriaphages • 1944: DNA is shown to be the building block of the gene • 1949: Pauling proves that sickle cell anemia is a "molecular disease" caused by a mutation ...
... characterizes viruses that infect bacteria, called bacteriaphages • 1944: DNA is shown to be the building block of the gene • 1949: Pauling proves that sickle cell anemia is a "molecular disease" caused by a mutation ...
Molecular Biology of the Cell
... amphibian oocyte. •A special form of chromosomes, found in oocytes of most animals (except mammals). •Seen at early meiosis stage due to an active transcription of many genes. •DNA are organized into a series of large chromatin loops, emanating from a chromosomal axis. Early in oocyte differentiatio ...
... amphibian oocyte. •A special form of chromosomes, found in oocytes of most animals (except mammals). •Seen at early meiosis stage due to an active transcription of many genes. •DNA are organized into a series of large chromatin loops, emanating from a chromosomal axis. Early in oocyte differentiatio ...
Treatment of lactose intolerance via β-galactosidase - Blogs at H-SC
... lactase gene could be delivered and incorporated into the host’s genome. Attempts at gene therapy have used adenoviruses and adeno-associated virus (AAV), which would behave as non-pathogenic viral vectors to deliver a corrected copy of a desired gene to the recipient. Unfortunately, this approach i ...
... lactase gene could be delivered and incorporated into the host’s genome. Attempts at gene therapy have used adenoviruses and adeno-associated virus (AAV), which would behave as non-pathogenic viral vectors to deliver a corrected copy of a desired gene to the recipient. Unfortunately, this approach i ...
Chapter 12
... non-LTR retrotransposons no long terminal repeats long interspersed elements short interspersed elements Alu I family 300 bp 106 copies 11% Human DNA ...
... non-LTR retrotransposons no long terminal repeats long interspersed elements short interspersed elements Alu I family 300 bp 106 copies 11% Human DNA ...
Glossary of Genetic Terms 11Jul15
... Cellulose - A sugar-rich compound of cell walls of plants and some protoctists CentiMorgan (cM) - (1) A measure of genetic distance that tells how far apart two genes are. Generally one centimorgan equals about 1 million base pairs. (2) A unit used to express distances on a genetic map. In genetic m ...
... Cellulose - A sugar-rich compound of cell walls of plants and some protoctists CentiMorgan (cM) - (1) A measure of genetic distance that tells how far apart two genes are. Generally one centimorgan equals about 1 million base pairs. (2) A unit used to express distances on a genetic map. In genetic m ...
genes, pseudogenes, deletions, insertion elements and DNA islands
... the aroG gene through to the beginning of opcB and 90% from the end of the opcB gene to comEA. Thus, large portions of the two regions were strongly homologous within each species and between both species. The Opc protein family The opcA region of Ng strains FA1090 and MS11 contains an opcA-like gen ...
... the aroG gene through to the beginning of opcB and 90% from the end of the opcB gene to comEA. Thus, large portions of the two regions were strongly homologous within each species and between both species. The Opc protein family The opcA region of Ng strains FA1090 and MS11 contains an opcA-like gen ...
Transgenic and gene disruption techniques from a concept to a tool
... prokaryotic organism or cultured eukaryotic cells, genetic modification of a multicellular organism is a complex and inefficient process. In the last decade three approaches have evolved in manipulating the mammalian genome: (1) introduction of complete transcriptional unit or minigene into the germ ...
... prokaryotic organism or cultured eukaryotic cells, genetic modification of a multicellular organism is a complex and inefficient process. In the last decade three approaches have evolved in manipulating the mammalian genome: (1) introduction of complete transcriptional unit or minigene into the germ ...
RACC BIO transcription and translation
... • Point mutations can affect protein structure and function • Mutations o Are changes in the genetic material of a cell that may involve large portions of a chromosome or just one base pair of nucleotides, which are Point mutations • Point mutations o Are changes in just one base pair of a gene ...
... • Point mutations can affect protein structure and function • Mutations o Are changes in the genetic material of a cell that may involve large portions of a chromosome or just one base pair of nucleotides, which are Point mutations • Point mutations o Are changes in just one base pair of a gene ...
Bio499 Bioinformatics
... on the sequence from _________ primer (choose one: forward or reverse). Use Workbench to ‘reverse and complement’ and then save the sequence. 3. Use “Merger” from my link to merge the two sequences. Remember one of the sequences has to be reverse-complement so both DNAs are in the same orientation. ...
... on the sequence from _________ primer (choose one: forward or reverse). Use Workbench to ‘reverse and complement’ and then save the sequence. 3. Use “Merger” from my link to merge the two sequences. Remember one of the sequences has to be reverse-complement so both DNAs are in the same orientation. ...
video slide - Greensburg
... • In a eukaryotic cell, the nuclear envelope separates transcription from translation • Eukaryotic RNA transcripts are modified through RNA processing to yield finished mRNA • Cells are governed by a cellular chain of command: DNA RNA protein ...
... • In a eukaryotic cell, the nuclear envelope separates transcription from translation • Eukaryotic RNA transcripts are modified through RNA processing to yield finished mRNA • Cells are governed by a cellular chain of command: DNA RNA protein ...
Example-Abstract
... biochemical analysis and find it to be the same as described before by genetic studies. Human and Saccharomyces cerevisiae RNASEH2A/Rnh201p subunits contain the catalytic center and are similar to each other and to prokaryotic RNase HII, which is functionally active as a single polypeptide. Although ...
... biochemical analysis and find it to be the same as described before by genetic studies. Human and Saccharomyces cerevisiae RNASEH2A/Rnh201p subunits contain the catalytic center and are similar to each other and to prokaryotic RNase HII, which is functionally active as a single polypeptide. Although ...
Adenine - One of the four bases in DNA tha make up the letters
... Cellulose - A sugar-rich compound of cell walls of plants and some protoctists CentiMorgan (cM) - (1) A measure of genetic distance that tells how far apart two genes are. Generally one centimorgan equals about 1 million base pairs. (2) A unit used to express distances on a genetic map. In genetic m ...
... Cellulose - A sugar-rich compound of cell walls of plants and some protoctists CentiMorgan (cM) - (1) A measure of genetic distance that tells how far apart two genes are. Generally one centimorgan equals about 1 million base pairs. (2) A unit used to express distances on a genetic map. In genetic m ...
mutations
... 1. Alkylation of guanine causes the breakage of the n-glycosidic bond of this purine from deoxyribose of the DNA backbone a) Can be repaired by the n-glycosidic endonuclease repair mechanism (1) Apurinic glycosylase breaks phosphodiester bond of DNA backbone when sugar is lacking its purine base ...
... 1. Alkylation of guanine causes the breakage of the n-glycosidic bond of this purine from deoxyribose of the DNA backbone a) Can be repaired by the n-glycosidic endonuclease repair mechanism (1) Apurinic glycosylase breaks phosphodiester bond of DNA backbone when sugar is lacking its purine base ...
Novel Roles for Selected Genes in Meiotic DNA Processing
... High-throughput studies of the 6,200 genes of Saccharomyces cerevisiae have provided valuable data resources. However, these resources require a return to experimental analysis to test predictions. An in-silico screen, mining existing interaction, expression, localization, and phenotype datasets was ...
... High-throughput studies of the 6,200 genes of Saccharomyces cerevisiae have provided valuable data resources. However, these resources require a return to experimental analysis to test predictions. An in-silico screen, mining existing interaction, expression, localization, and phenotype datasets was ...
Bacillus Subtilis Expression Vectors
... subtilis fused to the lac operator allowing their induction by addition of ITPG. While the background level of expression of these expression cassettes is very low in the absence of the inducer, an induction factor of about 1,300 was measured using the bgaB reporter gene (Phan et al., 2005). The amo ...
... subtilis fused to the lac operator allowing their induction by addition of ITPG. While the background level of expression of these expression cassettes is very low in the absence of the inducer, an induction factor of about 1,300 was measured using the bgaB reporter gene (Phan et al., 2005). The amo ...
BCH-201:Nucleotides and Nucleic acids
... Nucleic acids are defined as biopolymers that are involved in the preservation/storage and transmission of genetic information from one generation to another.The nucleotides that make up the nucleic acids are linked by phosphodiester bonds between 3’ and 5’ positions of the sugars. The linkage is ca ...
... Nucleic acids are defined as biopolymers that are involved in the preservation/storage and transmission of genetic information from one generation to another.The nucleotides that make up the nucleic acids are linked by phosphodiester bonds between 3’ and 5’ positions of the sugars. The linkage is ca ...
lecture1
... Nucleic acids are defined as biopolymers that are involved in the preservation/storage and transmission of genetic information from one generation to another.The nucleotides that make up the nucleic acids are linked by phosphodiester bonds between 3’ and 5’ positions of the sugars. The linkage is ca ...
... Nucleic acids are defined as biopolymers that are involved in the preservation/storage and transmission of genetic information from one generation to another.The nucleotides that make up the nucleic acids are linked by phosphodiester bonds between 3’ and 5’ positions of the sugars. The linkage is ca ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.