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Bacteria Reproduction
Bacteria Reproduction

... Bacteria reproduce through a process called binary fission. During binary fission, the chromosome copies itself, forming two genetically identical copies. Then, the cell enlarges and divides into two new daughter cells. The two daughter cells are identical to the parent cell. Binary fission can happ ...
LP - Columbia University
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... Prophase: this stage is reached when you can see chromosomes (as opposed to just chromatin) and nuclear membrane starts to break down. Chromosomes are doubled (2 chromatids/chromosome) but the two sister chromatids can stick together and appear as a single unit. So chromosomes may not look doubled ( ...
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... From the PHAC e-learning module: ‘For recombinant organisms. The parental and modified pathogens should be considered together when examining each of the risk factors. Risk group assessments for recombinant organisms require the researcher to consider the effect of the modification in the context of ...
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You may not start to read the questions printed on the subsequent

... additional copies of upstream genes and upregulating a transcription factor responsible for the expression ofthe pathway. They reduced flux to byproducts by replacing the promoter for the enzyme at that step with a repressible one. In the muconic acid pathway, then, you would introduce the foreign g ...
definitive non definitive non-invasive invasive prenatal diagnosis
definitive non definitive non-invasive invasive prenatal diagnosis

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ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

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Human-Genetics-Concepts-and-Applications-9E

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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