• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
TGAC * Sequence Polymorphisms Module
TGAC * Sequence Polymorphisms Module

... Q.41: What is the function of the gene? CDH13 function is not fully understood. CDH13 protein may act as a coreceptor for a signaling receptor through which adiponectin transmits metabolic signals. Q.42: What is the length of the protein that the gene encodes? 731 amino acids Q.43: What is the lengt ...
deschamp_2009_sequencing
deschamp_2009_sequencing

... Filtering, to accept clusters with only two members (A, B) with exactly one mismatch Recovering matched HQ unitag sequences and SNP sites from Vmatch alignments ...
Chapter 10
Chapter 10

... – Viruses exhibit some, but not all, characteristics of living organisms. Viruses: ...
TNT SP6 High-Yield Wheat Germ Protein Expression
TNT SP6 High-Yield Wheat Germ Protein Expression

IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)

... It is documented that large intragenic deletions and duplications together account for more than twothirds of the mutations leading to DMD and BMD and, despite heterogeneity in deletion size and location, two hot spots have been identified. Of which, the major one involves exons 40-55 [27, 28]. Thes ...
(lectures 24
(lectures 24

... 3. Immediately after an inversion occurs, or after an inversion chromosome is passed on to an individual which also has a normal, uninverted chromosome, the individual is an inversion heterozygote (its chromosomes having two different gene orders – the individual genes may or may not be heterozygous ...
Domain Bacteria
Domain Bacteria

...  Most bacteria thrive in pHs between __________________________  __________________________ are bacteria that thrive in low pHs (below 6) Reproduction  Usually reproduce by _________________________  ________________________________  ________________________________  DNA molecules move to ____ ...
Biology II - Grant County Schools
Biology II - Grant County Schools

... nucleotide nuclein blender experiment genetic recombination bacteriophage X-ray diffraction ...
Ch 6 Powerpoint
Ch 6 Powerpoint

... the same time.  ABO blood type is an example of this with both the dominant A and B being expressed in a type AB person. ...
GENECLEAN® Kit
GENECLEAN® Kit

... DNA generally binds to silica in high concentrations of chaotropic salt and elutes when the salt concentration is lowered. The mechanism of DNA binding to silica in high salt has not been completely described, but may involve chaotropic salt disruption of the water structure around negatively charge ...
The study of threshold determination of gene identification and its
The study of threshold determination of gene identification and its

Ironing Out Jesse`s Problem
Ironing Out Jesse`s Problem

... Student correctly determines the answer and expresses it in a fraction, decimal, or percent. ...
to the complete text - David Moore`s World of Fungi
to the complete text - David Moore`s World of Fungi

... Obviously, the recombinant progeny amount to 50% of the total, yet we know, because we set this feature at the outset, that these genes are on different chromosomes. A consequence of this is that if we cross two unknown genes together and get a close to 50% recombinant genotypes we can only describe ...
Bacterial Screening PCR Kit
Bacterial Screening PCR Kit

... In order to maintain consumer trust in the safety of food products, a high priority has been placed on assuring product quality at each step of the food supply process. PCR (Polymerase Chain Reaction) process is recognized as one of the more useful methods for such food-related quality control appli ...
Looking at long molecules in solution: what happens when they are
Looking at long molecules in solution: what happens when they are

... Many molecules in biological systems are long (e.g. DNA) or part of long assemblies of molecules (e.g. fibrous proteins or membrane-bound molecules). While it is clear that the structures of such moieties are important to their function, many of the powerful techniques of structural biology, includin ...
CHAPTER 6
CHAPTER 6

... Classes of Nucleic Acids? • DNA - one type, one purpose: - a single DNA molecules in virus and bacteria - Eukaryotic cells have many diploid chromosomes mainly in nucleus, but also mitochondria and chloroplasts. • RNA - 3 (or 4) types, 3 (or 4) purposes – ribosomal RNA - the basis of structure and f ...
Genetic analysis of acidocin B, a novel bacteriocin
Genetic analysis of acidocin B, a novel bacteriocin

... (Tagg e t al., 1976). The majority of bacteriocins produced by LAB can be classified into four distinct groups based on biochemical and genetic characteristics : (i) small ...
Electrophoresis and Hardy Wienberg notes
Electrophoresis and Hardy Wienberg notes

... These are STR sequences from two separate people, each block on the ‘string’ represents one repeat of an STR sequence on a chromosome – for this example, we’ll say the sequence is GATA. Notice that the mother has two repeats of the GATA sequence on one chromosome and three on the other, while the fa ...
Protocols for 16S rDNA Array Analyses of Microbial
Protocols for 16S rDNA Array Analyses of Microbial

here - Genomes Unzipped
here - Genomes Unzipped

... these two types of artifact in this analysis, as both are non-biological. For the test of position bias, it is known that the error rate of Illumina sequencing depends on the position in the read [6]. Additionally, mapping errors around insertions/deletions relative to a reference genome can lead to ...
Solution
Solution

... you  amplified  wild-­‐type  and  mutant  sickle  cell  anemia  alleles  by  PCR,  and  size-­‐separated   the  resulting  fragments,  would  you  be  able  to  distinguish  the  two  alleles?    Answer  yes  or   no,  and  explain  you ...
Co-dominant SCAR marker, P6-25 - Department of Plant Pathology
Co-dominant SCAR marker, P6-25 - Department of Plant Pathology

... 1 min. These cycles were followed by 72 C for 10 min, and then the reaction was held at 4 C. PCR reactions were performed in the MJ DNA Engine PT200 Thermocycler™ (MJ Research Inc., Waltham, MA). PCR-amplified fragments were separated by gel electrophoresis with 1.5% agarose in 0.5 X TBE buffer, sta ...
DNA-Directed Antibody Immobilization for
DNA-Directed Antibody Immobilization for

... chamber at 67% humidity. Following the overnight immobilization, the chips were washed with 50 mM ethanolamine in 1× Tris-buffered saline (150 mM NaCl and 50 mM Tris-HCl, Fisher Scientific), pH = 8.5, for 30 min to quench the remaining NHS groups in the polymer, then washed with PBST (PBS with 0.1% Tw ...
Epigenetic differences arise during the lifetime of
Epigenetic differences arise during the lifetime of

... genomic distribution of 5-methylcytosine DNA and histone acetylation, affecting their gene-expression portrait. These findings indicate how an appreciation of epigenetics is missing from our understanding of how different phenotypes can be originated from the same genotype. DNA methylation 兩 epigene ...
Quantitative Real-Time PCR for Non-invasive Rapid and
Quantitative Real-Time PCR for Non-invasive Rapid and

... subjects (n=15), and normal controls (n=10) that were tested by quantitative realtime PCR. This technique was applied by a MGB TaqMan probe based real-time PCR assay for rapid diagnosis of monosomy X-linked status in Turner syndrome. In the present study, we have measured and determined the gene dos ...
< 1 ... 102 103 104 105 106 107 108 109 110 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report