NucleoSpin 96 Flash Plasmid and Large-Construct DNA
... soluble proteins and other cell constituents. Afterwards, all precipitates are removed by filtration of the lysate through the NucleoSpin® 96 Flash Filter Plate under vacuum or in a suitable centrifuge for microtiter plates. Plasmid DNA is precipitated by addition of isopropanol to the filtrate and ...
... soluble proteins and other cell constituents. Afterwards, all precipitates are removed by filtration of the lysate through the NucleoSpin® 96 Flash Filter Plate under vacuum or in a suitable centrifuge for microtiter plates. Plasmid DNA is precipitated by addition of isopropanol to the filtrate and ...
Structure of DIG
... Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
... Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
Homologous Recombination 1. Query: Could you explain what
... The two duplexes taking part in the exchange are homologous chromosomes (one paternal and the other maternal). They are largely identical but have some differences, for example, in the region of interest, one carries M and the other m. This is what we designate as blue duplex (say M/M on the two str ...
... The two duplexes taking part in the exchange are homologous chromosomes (one paternal and the other maternal). They are largely identical but have some differences, for example, in the region of interest, one carries M and the other m. This is what we designate as blue duplex (say M/M on the two str ...
RT-PCR Master Mix (2X)
... or forward primer is complementary to the corresponding first strand of cDNA. In reverse transcription, the reverse primer initiates synthesis of the first strand of cDNA. In PCR, the forward primer initiates synthesis of the second strand of cDNA during the first PCR cycle. Both primers then exponenti ...
... or forward primer is complementary to the corresponding first strand of cDNA. In reverse transcription, the reverse primer initiates synthesis of the first strand of cDNA. In PCR, the forward primer initiates synthesis of the second strand of cDNA during the first PCR cycle. Both primers then exponenti ...
Chapter 8
... Enhancers: regulatory sequences located farther away from the start site. First identified in studies of the promoter of virus SV40. Activity of enhancers does not depend on their distance from, or orientation with respect to the transcription ...
... Enhancers: regulatory sequences located farther away from the start site. First identified in studies of the promoter of virus SV40. Activity of enhancers does not depend on their distance from, or orientation with respect to the transcription ...
Functional Requirements of the Yellow Fever Virus Capsid Protein
... functions to protect the E protein from premature fusion during virion morphogenesis (42). By themselves, the E and (pr)M proteins are able to assemble into noninfectious virus-like particles (VLPs). VLPs are small T⫽1 particles that are normally produced during flavivirus infectious (11, 25). Simil ...
... functions to protect the E protein from premature fusion during virion morphogenesis (42). By themselves, the E and (pr)M proteins are able to assemble into noninfectious virus-like particles (VLPs). VLPs are small T⫽1 particles that are normally produced during flavivirus infectious (11, 25). Simil ...
NSPCD LABORATORIES - World Health Organization
... Interpretation of typing results Multiple isolates representing a single type are most appropriately designated “indistinguishable” No typing method confirms that entire genomes of two organisms are identical Indistinguishable vs. closely related vs. possibly related →Final assessment lies with int ...
... Interpretation of typing results Multiple isolates representing a single type are most appropriately designated “indistinguishable” No typing method confirms that entire genomes of two organisms are identical Indistinguishable vs. closely related vs. possibly related →Final assessment lies with int ...
Threading-based Protein Structure Prediction
... need ~15 occurrences of each X-mer For tricodon-based models, need at least ...
... need ~15 occurrences of each X-mer For tricodon-based models, need at least ...
Regulation of Gene Expression
... • Function: It is the binding site for CTF1 (CAATbinding transcription factor) and C/EBP. The DNA-binding domain of TF1 is rich in basic Aas, and most likely it is in the alpha-helical conformation. C/EBP binds to DNA in a dimer known as leucine zipper. • Eukaryotes frequently have CAAT boxes, a str ...
... • Function: It is the binding site for CTF1 (CAATbinding transcription factor) and C/EBP. The DNA-binding domain of TF1 is rich in basic Aas, and most likely it is in the alpha-helical conformation. C/EBP binds to DNA in a dimer known as leucine zipper. • Eukaryotes frequently have CAAT boxes, a str ...
currently Final program
... WD2101 (order Tepidisphaerales). Members of the family Gemmataceae are also common in boreal and tundra wetlands. Although several peat-inhabiting planctomycetes have been obtained in pure cultures, little is known about their potential functions in the environment. In order to get insights into the ...
... WD2101 (order Tepidisphaerales). Members of the family Gemmataceae are also common in boreal and tundra wetlands. Although several peat-inhabiting planctomycetes have been obtained in pure cultures, little is known about their potential functions in the environment. In order to get insights into the ...
REAL-TIME PCR
... fluorescein (from the donor probe) is directly transferred to the acceptor dye by FRET. The acceptor fluorophore emits light at a different wavelength. Subsequently the fluorescent signal can be detected and measured. This happens during the annealing phase and first part of the extension phase of t ...
... fluorescein (from the donor probe) is directly transferred to the acceptor dye by FRET. The acceptor fluorophore emits light at a different wavelength. Subsequently the fluorescent signal can be detected and measured. This happens during the annealing phase and first part of the extension phase of t ...
Observations on the role of nuclear medicine in molecular imaging
... CT and MRI are morphological technologies capable of superb spatial resolution. While CT depends upon tissue density (i.e., selective absorption of X-rays), MRI depends upon differences in the chemical environment (i.e., magnetic relaxation) surround water protons and several other nuclei in tissues ...
... CT and MRI are morphological technologies capable of superb spatial resolution. While CT depends upon tissue density (i.e., selective absorption of X-rays), MRI depends upon differences in the chemical environment (i.e., magnetic relaxation) surround water protons and several other nuclei in tissues ...
Pvlea-18, a Member of a New Late-Embryogenesis
... review, see Dure, 1993a; Ingram and Bartles, 1996). Other hypothetical roles assigned to the LEA proteins are sequestration of ions (Dure, 1993b), molecular chaperone activity (Close, 1996), and transport of nuclear-targeted proteins during stress (Goday et al., 1994). Based on regions of significan ...
... review, see Dure, 1993a; Ingram and Bartles, 1996). Other hypothetical roles assigned to the LEA proteins are sequestration of ions (Dure, 1993b), molecular chaperone activity (Close, 1996), and transport of nuclear-targeted proteins during stress (Goday et al., 1994). Based on regions of significan ...
Azospirillum and related microorganisms.
... and leaves from different plants in rather high numbers. Interestingly, it could be demonstrated that the mild plant pathogenic Pseudomonas rubrislibalbicans is related to Herbaspirillum seropedicae on the genus level (Gillis et al. 1991, Hartmann et al., 1994). Molecular taxonomy is a powerful tool ...
... and leaves from different plants in rather high numbers. Interestingly, it could be demonstrated that the mild plant pathogenic Pseudomonas rubrislibalbicans is related to Herbaspirillum seropedicae on the genus level (Gillis et al. 1991, Hartmann et al., 1994). Molecular taxonomy is a powerful tool ...
10 Fungal Genetics Newsletter mcm
... Generation of hygromycin resistance transformants: The wild type strain of N. crassa OR23-I V A (FGSC 2489) was transformed by electroporation (Gene Pulsar II, BIORAD) with the plasmid pCSN44 containing the hph gene which encodes for hygromycin resistance (Staben et al. 1989 Fungal Genet. Newsl. 36: ...
... Generation of hygromycin resistance transformants: The wild type strain of N. crassa OR23-I V A (FGSC 2489) was transformed by electroporation (Gene Pulsar II, BIORAD) with the plasmid pCSN44 containing the hph gene which encodes for hygromycin resistance (Staben et al. 1989 Fungal Genet. Newsl. 36: ...
ISOLATE II PCR and Gel Kit
... PCR primers from reactions are eliminated while small DNA fragments are still bound and purified with high recovery. The cut-off for small DNA fragments can be shifted from <50bp to several hundred base pairs by diluting Binding Buffer CB to remove primer-dimers from target PCR products. A yellow pH ...
... PCR primers from reactions are eliminated while small DNA fragments are still bound and purified with high recovery. The cut-off for small DNA fragments can be shifted from <50bp to several hundred base pairs by diluting Binding Buffer CB to remove primer-dimers from target PCR products. A yellow pH ...
this lecture as PDF here
... phage DNA or RNA is stored) and tunnel tails, the tips of which have the ability to bind to specific molecules on the surface of their target bacteria. The viral DNA is then injected through the tail into the host cell, where it directs the production of progeny phages often over a hundred in half a ...
... phage DNA or RNA is stored) and tunnel tails, the tips of which have the ability to bind to specific molecules on the surface of their target bacteria. The viral DNA is then injected through the tail into the host cell, where it directs the production of progeny phages often over a hundred in half a ...
Optimality models in biology nanocourse @ Harvard
... See also: Alon, Biological networks - the tinkerer as an engineer, Science (2003) ...
... See also: Alon, Biological networks - the tinkerer as an engineer, Science (2003) ...
Foundations of Biology - Geoscience Research Institute
... 1 To every thing there is a season, and a time to every purpose under the heaven: ©2000 Timothy G. Standish ...
... 1 To every thing there is a season, and a time to every purpose under the heaven: ©2000 Timothy G. Standish ...