Biochemistry
... Proteins are the most complex macromolecules in the cell. They are composed of linear polymers called polypeptides, which contain amino acids connected by peptide bonds. ...
... Proteins are the most complex macromolecules in the cell. They are composed of linear polymers called polypeptides, which contain amino acids connected by peptide bonds. ...
Biotechnology and Bioinformatics
... represents portions of expressed genes. This technique allows for a comparison ESTs from lesser-known species with those from a species whose genome has been well-characterized enabling researchers to identify the function of gene loci in the new organism. ...
... represents portions of expressed genes. This technique allows for a comparison ESTs from lesser-known species with those from a species whose genome has been well-characterized enabling researchers to identify the function of gene loci in the new organism. ...
Total RNA MinElute Cleanup - Yale Center for Genome Analysis
... 4. Transfer RNeasy MinElute column into a new 2 ml collection tube. Save flow through until sample quantitation is completed. 5. Pipet 500 μl of Buffer RPE onto column. Spin for 15 sec at ≥10,000 rpm. Discard flowthrough. 6. Pipette 500 μl of 80% ethanol to column. Centrifuge for 2 min at ≥10,000 rp ...
... 4. Transfer RNeasy MinElute column into a new 2 ml collection tube. Save flow through until sample quantitation is completed. 5. Pipet 500 μl of Buffer RPE onto column. Spin for 15 sec at ≥10,000 rpm. Discard flowthrough. 6. Pipette 500 μl of 80% ethanol to column. Centrifuge for 2 min at ≥10,000 rp ...
Gene Section FGA7 (Fused Gene 7 to AML1) in Oncology and Haematology
... The predicted AML1-FGA7 chimeric proteins contain a limited number of amino acid residues fused to AML1 in a situation similar to that reported for AML1EAP fusion that is a product of t(3;21). It is possible that the expression of a constitutively shortened AML1 could compete with full-length AML1 a ...
... The predicted AML1-FGA7 chimeric proteins contain a limited number of amino acid residues fused to AML1 in a situation similar to that reported for AML1EAP fusion that is a product of t(3;21). It is possible that the expression of a constitutively shortened AML1 could compete with full-length AML1 a ...
Supporting Information File SF5
... guanidine thiocyanate and phenol in mono-phase solution, the thymus tissues were homogenized in TRI REAGENT (1 ml per 50-100 mg of tissue) in a Polytron homogenizer and light vertex and incubation (10 min, room temp). 0.2 ml of chloroform was added per ml of TRI REAGENT and incubation (10 min, room ...
... guanidine thiocyanate and phenol in mono-phase solution, the thymus tissues were homogenized in TRI REAGENT (1 ml per 50-100 mg of tissue) in a Polytron homogenizer and light vertex and incubation (10 min, room temp). 0.2 ml of chloroform was added per ml of TRI REAGENT and incubation (10 min, room ...
Lecture 15: Translation and Transcription
... Prokaryotes have a single type of polymerase that synthesizes all types of RNA (e.g., tRNA, rRNA, mRNA) b. Eukaryotes have three types: i. RNA polymerase II catalyzes mRNA synthesis Transcription unit a. A specific sequence of DNA that is transcribed into a single mRNA molecule b. Includes sequences ...
... Prokaryotes have a single type of polymerase that synthesizes all types of RNA (e.g., tRNA, rRNA, mRNA) b. Eukaryotes have three types: i. RNA polymerase II catalyzes mRNA synthesis Transcription unit a. A specific sequence of DNA that is transcribed into a single mRNA molecule b. Includes sequences ...
Document
... polarity of T blast cell divisions? Why. (10) c) The lin-44 gene is expressed (transcribed) specifically in the hyp8/9 and hyp11 cells. Does this information help explain the results in examples 2, 3, 4 and why? (10) 7.) The properties that a molecule needs to satisfy to be considered a morphogen ar ...
... polarity of T blast cell divisions? Why. (10) c) The lin-44 gene is expressed (transcribed) specifically in the hyp8/9 and hyp11 cells. Does this information help explain the results in examples 2, 3, 4 and why? (10) 7.) The properties that a molecule needs to satisfy to be considered a morphogen ar ...
Applications of recombinant DNA technology in
... eukaryotic cells. Genes that encode proteins are transcribed into messenger RNA (mRNA) by RNA polymerase II. Ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs) are transcribed by RNA polymerase I and III, respectively. Some small nuclear and cytoplasmic RNAs are transcribed by RNA polymerase II and II ...
... eukaryotic cells. Genes that encode proteins are transcribed into messenger RNA (mRNA) by RNA polymerase II. Ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs) are transcribed by RNA polymerase I and III, respectively. Some small nuclear and cytoplasmic RNAs are transcribed by RNA polymerase II and II ...
Biological Chemistry II: Problem Set 1
... identical length, if you assume that all 20 proteinogenic amino acids occur with equal frequency and are distributed uniformly over the length of the protein? (c) BLAST searches are performed to identify proteins having similar amino acid sequences. A BLAST tutorial can be found at http://www.ncbi.n ...
... identical length, if you assume that all 20 proteinogenic amino acids occur with equal frequency and are distributed uniformly over the length of the protein? (c) BLAST searches are performed to identify proteins having similar amino acid sequences. A BLAST tutorial can be found at http://www.ncbi.n ...
11060_2014_1398_MOESM3_ESM
... Exon 4 genome DNA isolated of each sample was submitted to PCR amplification for exon 4 of both IDH1 and IDH2 genes, using the Platinum DNA Polymerase (5,0U) (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instruction. Primers were designed with the assistance of Primer Express Software ...
... Exon 4 genome DNA isolated of each sample was submitted to PCR amplification for exon 4 of both IDH1 and IDH2 genes, using the Platinum DNA Polymerase (5,0U) (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instruction. Primers were designed with the assistance of Primer Express Software ...
PURExpress® Δ Ribosome Kit
... 1. The DHFR control template is now supplied at 125 ng/µl. Use 2 µl for the positive control reaction. We use 60 pmoles of ribosomes in a standard 25 μl reaction. The supplied control ribosomes are enough for two reactions. Note: Using a smaller amount of ribosomes is possible but the protein yield ...
... 1. The DHFR control template is now supplied at 125 ng/µl. Use 2 µl for the positive control reaction. We use 60 pmoles of ribosomes in a standard 25 μl reaction. The supplied control ribosomes are enough for two reactions. Note: Using a smaller amount of ribosomes is possible but the protein yield ...
Unit 4 Checklist of Knowledge File
... modifications, to include addition of a poly-A tail, a GTP cap, and the excision of introns Translation of mRNA occurs in the cytoplasm on the ribosome. In prokaryotic organisms, transcription is coupled to translation of the message. Translation involves energy and many steps, including initiation, ...
... modifications, to include addition of a poly-A tail, a GTP cap, and the excision of introns Translation of mRNA occurs in the cytoplasm on the ribosome. In prokaryotic organisms, transcription is coupled to translation of the message. Translation involves energy and many steps, including initiation, ...
Slide 1
... The lac operon When an E. coli encounters lactose, all the enzymes needed for its metabolism are made at once using the lactose operon. – In the absence of lactose, the repressor binds to the operator and prevents RNA polymerase action. – In presence of lactose, lactose inactivates the repressor, ...
... The lac operon When an E. coli encounters lactose, all the enzymes needed for its metabolism are made at once using the lactose operon. – In the absence of lactose, the repressor binds to the operator and prevents RNA polymerase action. – In presence of lactose, lactose inactivates the repressor, ...
Gene Ontology Annotation (UniProt-GOA) - EMBL-EBI
... Manual annotation benefits Manual annotation provides the most specific and detailed annotations for gene products. One of our aims is to undertake focused annotation projects, to improve both the ontology and its association to gene products. Recent examples of this include annotation of proteins i ...
... Manual annotation benefits Manual annotation provides the most specific and detailed annotations for gene products. One of our aims is to undertake focused annotation projects, to improve both the ontology and its association to gene products. Recent examples of this include annotation of proteins i ...
Microbiology Chapter 9
... DNA replication – the process of making an exact copy of DNA molecule DNA replication has to occur before cell division in order for each cell to have a copy of the genetic information 1. One strand of DNA acts as template for the formation of the corresponding strand 2. 2. Replication is carried ou ...
... DNA replication – the process of making an exact copy of DNA molecule DNA replication has to occur before cell division in order for each cell to have a copy of the genetic information 1. One strand of DNA acts as template for the formation of the corresponding strand 2. 2. Replication is carried ou ...
Lab
... • For proteins a different scoring matrix used, optimized for smaller evolutionary distances ...
... • For proteins a different scoring matrix used, optimized for smaller evolutionary distances ...
Cellular and Molecular Biology (HTH SCI 1I06) Legacy Summary
... RNA translation is the next step in protein synthesis. Translation involves the building of an amino acid chain from a modified version of the original RNA transcript (known as mRNA). In order to accomplish this, the mRNA is transported from the nucleus to ribosomes (functional units that build prot ...
... RNA translation is the next step in protein synthesis. Translation involves the building of an amino acid chain from a modified version of the original RNA transcript (known as mRNA). In order to accomplish this, the mRNA is transported from the nucleus to ribosomes (functional units that build prot ...
Executive Summary - Defra Science Search
... state of the marine environment require ever more sophisticated tools to detect cause and effect. 2. Biological measurements are an increasingly important part of monitoring and biomarkers provide evidence for exposure and indicate risk for adverse effects at individual and population level. 3. Hist ...
... state of the marine environment require ever more sophisticated tools to detect cause and effect. 2. Biological measurements are an increasingly important part of monitoring and biomarkers provide evidence for exposure and indicate risk for adverse effects at individual and population level. 3. Hist ...
Answer keyDNA Practice problems
... If you were to replicate the above strand of DNA in this direction ----, from left to right, which of the parent stands is used to build the leading strand of DNA? The lagging strand??? Here is a model for the above DNA strands: 3’-------------------------------5’----this is the parent strand which ...
... If you were to replicate the above strand of DNA in this direction ----, from left to right, which of the parent stands is used to build the leading strand of DNA? The lagging strand??? Here is a model for the above DNA strands: 3’-------------------------------5’----this is the parent strand which ...
Document
... 1. Which one of the following nucleotide pair bonds would be found in a DNA molecule? a. adenine-guanine c. adenine-cytosine b. guanine-cytosine d. cytosine-uracil 2. The backbone of a DNA molecule is made of which two components? a. phosphate molecules and ribose sugars b. deoxyphosphate molecules ...
... 1. Which one of the following nucleotide pair bonds would be found in a DNA molecule? a. adenine-guanine c. adenine-cytosine b. guanine-cytosine d. cytosine-uracil 2. The backbone of a DNA molecule is made of which two components? a. phosphate molecules and ribose sugars b. deoxyphosphate molecules ...
DNA Kit Instructions
... and binds there. This new strand detaches, and the original DNA strands pair up again and the helix shape reforms. The newly formed detached strand is called mRNA. It is an exact opposite of the original DNA strand that was copied. The mRNA strand leaves the nucleus and enters the cytoplasm, and fin ...
... and binds there. This new strand detaches, and the original DNA strands pair up again and the helix shape reforms. The newly formed detached strand is called mRNA. It is an exact opposite of the original DNA strand that was copied. The mRNA strand leaves the nucleus and enters the cytoplasm, and fin ...
DNA : The Genetic Material
... switch for transcription. (it aids in shielding the RNA polymerase binding site of a specific gene. • Operon: a group of genes that code for enzymes involved in the same function, their promoter site, and the operator that controls them. • The operon that controls the metabolism of lactose is called ...
... switch for transcription. (it aids in shielding the RNA polymerase binding site of a specific gene. • Operon: a group of genes that code for enzymes involved in the same function, their promoter site, and the operator that controls them. • The operon that controls the metabolism of lactose is called ...
Prescott`s Microbiology, 9th Edition Chapter 17 – Recombinant DNA
... Figure 17.4 Why might two DNA fragments inadvertently be cloned into a single vactor when using this cloning strategy? Two fragments of DNA from the organism could anneal together, making a concatemer, which would still have the two appropriate ends to be accepted into the vector, because both sites ...
... Figure 17.4 Why might two DNA fragments inadvertently be cloned into a single vactor when using this cloning strategy? Two fragments of DNA from the organism could anneal together, making a concatemer, which would still have the two appropriate ends to be accepted into the vector, because both sites ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.