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DNA How the Molecule of Heredity Carries, Replicates, and
DNA How the Molecule of Heredity Carries, Replicates, and

... Pol III – produces new stands of complementary DNA Pol I – fills in gaps between newly synthesized Okazaki segments DNA helicase – unwinds double helix Single-stranded binding proteins – keep helix open Primase – creates RNA primers to initiate synthesis Ligase – welds together Okazaki fragments Cop ...
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When epigenetics meets alternative splicing: the roles of DNA

... The process of pre-mRNA splicing has been studied for more than 30 years, yet it is far from being fully understood. Accumulating evidence suggests that many splicing events occur cotranscriptionally and that the mRNA precursor remains associated with the chromatin until all of the introns have been ...
DNA transcription 3.lecture ENG OK
DNA transcription 3.lecture ENG OK

Enhancing fairness in DNA jury trials
Enhancing fairness in DNA jury trials

Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides

McCance, J. An attempt at isolating and characterizing segmented
McCance, J. An attempt at isolating and characterizing segmented

... Cloning was carried out on the PCR product produced with the general bacterial primers using the TOPO cloning kit. The PCR product was first ligated by adding 2u1 of fresh PCR product, 2 ul of sterile water and lul PCR2.1-TOPO vector which was then incubated at room temperature for 5 minutes. A nega ...
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typing methods - Micro-Rao

... The process takes 2-4 days and requires costly reagents and equipment besides being labour intensive. 4. Southern blot analysis of RFLPs In contrast to REA of DNA, southern blot analyses detect only the particular restriction fragment. The DNA is digested by endonuclease, the fragments are separated ...
Chapter 9 From DNA to Protein
Chapter 9 From DNA to Protein

... • RNA is composed of a single-strand chain of nucleotides • An RNA nucleotide has three phosphate groups, a sugar, and one of four bases – Unlike DNA, the sugar is a ribose ...
101. The Role of Rigidity in DNA Looping
101. The Role of Rigidity in DNA Looping

... one domain, two basic modulation mechanisms are possible. First, the affinity of individual domains for DNA can be modulated, and, second, the joint affinity or binding cooperativity between the two units can be modulated. A simple way to accomplish the latter is for the ligand to shift the separati ...
DNA Mimic Proteins: Functions, Structures, and Bioinformatic Analysis
DNA Mimic Proteins: Functions, Structures, and Bioinformatic Analysis

Forensic DNA Fundamentals for the Prosecutor
Forensic DNA Fundamentals for the Prosecutor

... of a case. First, they can determine the genetic profile drawn from biological evidence found at a crime scene and match it to the genetic profile from a defendant, which would tie this defendant to this charged crime. Then, a scientist can calculate the statistical probability that a random unrelat ...
1-6 DNA Sp12
1-6 DNA Sp12

... Important because it revealed how DNA can be duplicated from cell to cell And led to the birth of molecular biology ...
Educator's Resource Guide 4226  Biology 1 s 4-5
Educator's Resource Guide 4226 Biology 1 s 4-5

... 11. Using the principle of independent assortment, complete the Punnett square to show the results of an F1 cross between two individuals heterozygous for both pod color (C = green and c = yellow) and pod shape (S = smooth and s + constricted). The gametes and some of the genotypes of the F2 offspri ...
Candidatus Liberibacter asiaticus - Southwest Florida Research and
Candidatus Liberibacter asiaticus - Southwest Florida Research and

Codon Dictionary Worksheet
Codon Dictionary Worksheet

... To the right is a codon dictionary. This is a listing of messenger RNA (mRNA) triplets that correspond to the anticodons found on transfer RNA (tRNA) and the amino acids that tRNA carries. Remember that the sense strand of DNA carries the original genetic code for each amino acid. DNA polymerase mat ...
Chapter 8 Human Chromosomes
Chapter 8 Human Chromosomes

... centromere location, and banding pattern. As menIn addition to their length, Cytogenetists can tioned before, these are called autosomes. However distinguish chromosomes using their centromere note that two of the chromosomes, the X and the Y, do position and banding pattern. Note that at the not lo ...
The Basics of RT-PCR
The Basics of RT-PCR

... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
Identification of a cis-Element That Determines Autonomous DNA
Identification of a cis-Element That Determines Autonomous DNA

View/Open - DukeSpace
View/Open - DukeSpace

... The sequences of DNA that comprise a gene are referred to as exons or exonic sequences. Most exonic sequences will code for a particular protein, but they also include other regulatory or non-coding regions that, although not coding for a particular protein, are important to the translation of mRNA. ...
Unit 3 Solutions - Manning`s Science
Unit 3 Solutions - Manning`s Science

... parents and maintain their genetic identity. ...
Pre-AP Biology
Pre-AP Biology

... What are the electron carriers used in photosynthesis and respiration? Why are electron carriers ...
An active foamy virus integrase is required for virus replication
An active foamy virus integrase is required for virus replication

... Foamy viruses (FVs) make use of a replication strategy which is unique among retroviruses and shows analogies to hepadnaviruses. The presence of an integrase (IN) and obligate provirus integration distinguish retroviruses from hepadnaviruses. To clarify whether a functional IN is required for FV rep ...
Mutation, repair, and recombination
Mutation, repair, and recombination

TRANSPOSON INSERTION SITE VERIFICATION
TRANSPOSON INSERTION SITE VERIFICATION

... Fax. +44 (0)1603 450021, mailto: [email protected] ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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