DNA Sequence Alignment - National Taiwan University
... On the whole, the BLAST has two versions- Original BLAST and New BLAST (Gapped BLAST). The original version of BLAST stretches a longer ungapped alignment (any gap is not allowed in the alignment) between the query and database sequence in left and right directions, form the position where exact mat ...
... On the whole, the BLAST has two versions- Original BLAST and New BLAST (Gapped BLAST). The original version of BLAST stretches a longer ungapped alignment (any gap is not allowed in the alignment) between the query and database sequence in left and right directions, form the position where exact mat ...
Sample pages 1 PDF
... The first cytologist who described chromosome behavior during cell division and how chromosomes move during mitosis was Walter Flemming (1882) in 1882. His terms “prophase,” “metaphase,” and “anaphase” are still used to describe the different steps of mitosis. In 1888 the structures were termed “chr ...
... The first cytologist who described chromosome behavior during cell division and how chromosomes move during mitosis was Walter Flemming (1882) in 1882. His terms “prophase,” “metaphase,” and “anaphase” are still used to describe the different steps of mitosis. In 1888 the structures were termed “chr ...
A MICROFLUIDIC CHIP COMBINING DNA EXTRACTION AND
... Figure 3: Real-time amplification plot for the S. aureus nuc gene. 10 µL of MSSA cell lysate was used as the sample for each well, while the presence of primers and probes was varied between wells. CONCLUSION Multiplexed PCR is usually done with all primer sets combined in a single reaction. It is ...
... Figure 3: Real-time amplification plot for the S. aureus nuc gene. 10 µL of MSSA cell lysate was used as the sample for each well, while the presence of primers and probes was varied between wells. CONCLUSION Multiplexed PCR is usually done with all primer sets combined in a single reaction. It is ...
AP® BIOLOGY 2009 SCORING GUIDELINES (Form B)
... The response is well written and organized according to the question. Key terms are underlined and followed with good descriptions of those terms. A total of 6 points were earned from the description of how a plasmid can be modified. The first point was earned for providing the definition of the pla ...
... The response is well written and organized according to the question. Key terms are underlined and followed with good descriptions of those terms. A total of 6 points were earned from the description of how a plasmid can be modified. The first point was earned for providing the definition of the pla ...
Dr. Apr. Dieter Deforce
... 2.B.5. Special precautions to be taken in performing PCR for diagnostic purposes: Contamination is one of the major reasons for misinterpretation of results with PCR and occurs when exogenous DNA or RNA, proteases, nucleases and various inhibitors of Taq polymerase are introduced into the reaction m ...
... 2.B.5. Special precautions to be taken in performing PCR for diagnostic purposes: Contamination is one of the major reasons for misinterpretation of results with PCR and occurs when exogenous DNA or RNA, proteases, nucleases and various inhibitors of Taq polymerase are introduced into the reaction m ...
Troubleshooting Guide for DNA Electrophoresis
... Following electrophoresis, visualize DNA by staining in ethidium bromide solution (final concentration 0.5 μg/ml) or SYBR® Green I. Do not exceed the recommended concentration of the dye for staining. Avoid prolonged staining for more than 30 min as this may result in high background. If the g ...
... Following electrophoresis, visualize DNA by staining in ethidium bromide solution (final concentration 0.5 μg/ml) or SYBR® Green I. Do not exceed the recommended concentration of the dye for staining. Avoid prolonged staining for more than 30 min as this may result in high background. If the g ...
Ch11_lecture students
... result of replication errors, ultraviolet light, chemicals, and many other environmental factors are called __________. • Sometimes during DNA replication, an incorrect pair of nucleotides is incorporated into the growing DNA double helix. • This is called nucleotide substitution, or point mutation, ...
... result of replication errors, ultraviolet light, chemicals, and many other environmental factors are called __________. • Sometimes during DNA replication, an incorrect pair of nucleotides is incorporated into the growing DNA double helix. • This is called nucleotide substitution, or point mutation, ...
RNA analysis on non-denaturing agarose gel electrophoresis
... 2. Heat an aliquot of the RNA solution at 70°C for 1 min and place it on ice before loading on a gel. 3. Load a known amount of DNA or RNA ladder alongside your RNA sample as a standard for determining the RNA concentration. RNA concentration can be roughly estimated assuming that the efficiency of ...
... 2. Heat an aliquot of the RNA solution at 70°C for 1 min and place it on ice before loading on a gel. 3. Load a known amount of DNA or RNA ladder alongside your RNA sample as a standard for determining the RNA concentration. RNA concentration can be roughly estimated assuming that the efficiency of ...
BioTeke Corporation
... Transfer the Spin-column AC to a clean tube, add 100μl Buffer EB (having been incubated at 65-70℃ water-bath), stand for 3-5 min in RT. Centrifuge at 12,000 rpm for 1 min. Take flow-through back the Spin-column AC, stand for 3-5 min in RT, centrifuge at 12,000 rpm for 1 min. More elution volume, mor ...
... Transfer the Spin-column AC to a clean tube, add 100μl Buffer EB (having been incubated at 65-70℃ water-bath), stand for 3-5 min in RT. Centrifuge at 12,000 rpm for 1 min. Take flow-through back the Spin-column AC, stand for 3-5 min in RT, centrifuge at 12,000 rpm for 1 min. More elution volume, mor ...
1. Translation
... For activator or repressor proteins to do their job, each must be able to exist in two states: one that can bind its DNA targets and one that cannot. The binding state must be in accord with the cellular environment; that is, be appropriate for a given set of physiological conditions. A site on the ...
... For activator or repressor proteins to do their job, each must be able to exist in two states: one that can bind its DNA targets and one that cannot. The binding state must be in accord with the cellular environment; that is, be appropriate for a given set of physiological conditions. A site on the ...
Replication timing as an epigenetic mark
... these segments remain in their respective sub-nuclear locations throughout interphase.18 Labeled foci, even adjacent chromosome segments that replicate less than two hours apart, remain distinct and retain their size and intensity for multiple generations.16,17 Intriguingly, the entire cohort of foc ...
... these segments remain in their respective sub-nuclear locations throughout interphase.18 Labeled foci, even adjacent chromosome segments that replicate less than two hours apart, remain distinct and retain their size and intensity for multiple generations.16,17 Intriguingly, the entire cohort of foc ...
On the feasibility of using network processors for DNA processing
... monitors that need to perform increasingly complex operations on data traffic at increasingly high link speeds. Indeed, to our knowledge, this has been the only application domain for NPUs to date. We observe, however, that bio-computing fields like DNA processing share many of the properties and pr ...
... monitors that need to perform increasingly complex operations on data traffic at increasingly high link speeds. Indeed, to our knowledge, this has been the only application domain for NPUs to date. We observe, however, that bio-computing fields like DNA processing share many of the properties and pr ...
Three Reversible Data Encoding Algorithms based on DNA and
... table 5 to get an amino acid character without a remainder. What is left is to give the information of the original size of the remainder to be used in decoding to extract the exact remainder and continue decoding. This is done by additional two DNA: “AC” represents the ‘one’ size and “AG” to repres ...
... table 5 to get an amino acid character without a remainder. What is left is to give the information of the original size of the remainder to be used in decoding to extract the exact remainder and continue decoding. This is done by additional two DNA: “AC” represents the ‘one’ size and “AG” to repres ...
Document
... the metabolites are called co-inducers Decreased synthesis in response to a metabolite is termed ‘repression’ and the metabolite a co-repressor Some substrates induce enzyme synthesis/expression even though the enzymes can’t metabolize the substrate o these examples are ‘gratuitous inducers’ (su ...
... the metabolites are called co-inducers Decreased synthesis in response to a metabolite is termed ‘repression’ and the metabolite a co-repressor Some substrates induce enzyme synthesis/expression even though the enzymes can’t metabolize the substrate o these examples are ‘gratuitous inducers’ (su ...
Slide 1
... And Wikipedia continues in this vein by describing 2 types of (IT-related) ontology: “A domain ontology (or domain-specific ontology) models a specific domain, or part of the world. It represents the particular meanings of terms as they apply to that domain.” (Wikipedia, 2008) “An upper ontology (o ...
... And Wikipedia continues in this vein by describing 2 types of (IT-related) ontology: “A domain ontology (or domain-specific ontology) models a specific domain, or part of the world. It represents the particular meanings of terms as they apply to that domain.” (Wikipedia, 2008) “An upper ontology (o ...
Expansion of tandem repeats and oligomer
... interest due to their role in genome organization and evolutionary processes [1–11]. It is known that SSR constitute a large fraction of noncoding DNA and are relatively rare in protein coding sequences. SSR are of considerable practical and theoretical interest due to their high polymorphism [7]. T ...
... interest due to their role in genome organization and evolutionary processes [1–11]. It is known that SSR constitute a large fraction of noncoding DNA and are relatively rare in protein coding sequences. SSR are of considerable practical and theoretical interest due to their high polymorphism [7]. T ...
embryos) (1). Smaller P elements are also present
... and Messing (12) during the construction of pUC8 to remove an Eco RI site. The origin of the 39 bp deletion is unclear; these nucleotides were deleted from Carnegie 1 as compared to the corresponding region of pBR322 (13). The Carnegie 1 DNA sequence shown was assembled as follows: The DNA sequence ...
... and Messing (12) during the construction of pUC8 to remove an Eco RI site. The origin of the 39 bp deletion is unclear; these nucleotides were deleted from Carnegie 1 as compared to the corresponding region of pBR322 (13). The Carnegie 1 DNA sequence shown was assembled as follows: The DNA sequence ...
8.1 Why Do Cells Divide?
... Duplicated chromosomes separate during cell division. • Prior to cell division, the DNA within each chromosome is replicated. • The duplicated chromosomes then consist of two DNA double helixes and associated proteins that are attached to each other at the centromere. Each of the duplicated chromo ...
... Duplicated chromosomes separate during cell division. • Prior to cell division, the DNA within each chromosome is replicated. • The duplicated chromosomes then consist of two DNA double helixes and associated proteins that are attached to each other at the centromere. Each of the duplicated chromo ...
Primer on Molecular Genetics
... genes. Interspersed within many genes are intron sequences, which have no coding function. The balance of the genome is thought to consist of other noncoding regions (such as control sequences and intergenic regions), whose functions are obscure. All living organisms are composed largely of proteins ...
... genes. Interspersed within many genes are intron sequences, which have no coding function. The balance of the genome is thought to consist of other noncoding regions (such as control sequences and intergenic regions), whose functions are obscure. All living organisms are composed largely of proteins ...
Chromosomal theory of inheritance
... attaches to the spindle fibres • The position of the centromere – Metacentric: in the middle – Acrocentric: off centre – Telocentric: at one end ...
... attaches to the spindle fibres • The position of the centromere – Metacentric: in the middle – Acrocentric: off centre – Telocentric: at one end ...
1 Transmission of genetic variation: conjugation Transmission of
... pCTX and the V. cholerae chromosome (green triangles) generates either a single CTX prophage or (as shown) tandem prophages. (e) Tandem prophages can serve as a template for production of extrachromosomal phage DNA. This process is initiated by the phageencoded protein RstA; (f) it results in the fo ...
... pCTX and the V. cholerae chromosome (green triangles) generates either a single CTX prophage or (as shown) tandem prophages. (e) Tandem prophages can serve as a template for production of extrachromosomal phage DNA. This process is initiated by the phageencoded protein RstA; (f) it results in the fo ...
A unique pattern of intrastrand anomalies in base
... The 50 non-coding bases immediately internal to the telomeric repeats in the two 5′ ends of macronuclear DNA molecules of a group of hypotrichous ciliates are anomalous in composition, consisting of 61% purines and 39% pyrimidines, A>T (ratio of 44:32), and G>C (ratio of 17:7). These ratio imbalance ...
... The 50 non-coding bases immediately internal to the telomeric repeats in the two 5′ ends of macronuclear DNA molecules of a group of hypotrichous ciliates are anomalous in composition, consisting of 61% purines and 39% pyrimidines, A>T (ratio of 44:32), and G>C (ratio of 17:7). These ratio imbalance ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.