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... Chapter 12. Transcriptional Activators in Eucaryotes Chapter 11. General Transcription Factors in Eucaryotes. ...
Fruit Salad—Hold the DNA, Please
Fruit Salad—Hold the DNA, Please

Investigation of the structure of DNA
Investigation of the structure of DNA

Protein Synthesis Introduction
Protein Synthesis Introduction

... The Flow of Genetic Information A gene is a segment of DNA that is located on a chromosome and that codes for a particular trait. The trait is expressed by the actions of the protein which is produced when the gene is used. For example, a person’s hair color is determined by a gene which directs the ...
XXII – DNA cloning and sequencing Outline
XXII – DNA cloning and sequencing Outline

... change of colonies grown on Xgal media. Other bacteria can also be excluded by use of ampicillin in media, as the pUC18 vector contains an ampicillin-resistance gene. ...
Proteins
Proteins

... perform specific functions for the organism; therefore, individual cells differentiate and become specialized in structure and function.  Differentiation happens due to selective gene expression – some genes are turned off, some are ...
Mutations
Mutations

A closer look at Transcription and Translation
A closer look at Transcription and Translation

Biochemistry WebQuest - For the love of Science!
Biochemistry WebQuest - For the love of Science!

Nucleic acids store and transmit hereditary information
Nucleic acids store and transmit hereditary information

... DNA double helix • An RNA molecule is single polynucleotide chain. • DNA molecules have two polynucleotide strands that spiral around an imaginary axis to form a double helix. – The double helix was first proposed as the structure of DNA in 1953 by James Watson and Francis Crick. ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... • In 2006 a crystal structure with GTP rather than UTP in the A site, opposite a C, revealed a part of Rpb1 roughly encompassing residues 1070 to 1100 - a trigger loop • The trigger loop only comes into play when the correct substrate occupies the A site and makes several important contacts with the ...
Transcription and Translation
Transcription and Translation

...  In your groups of four (each table should work with the one behind ...
I INTRODUCTION Deoxyribonucleic Acid (DNA), genetic material of
I INTRODUCTION Deoxyribonucleic Acid (DNA), genetic material of

... material of all cellular organisms and most viruses. DNA carries the information needed to direct protein synthesis and replication. Protein synthesis is the production of the proteins needed by the cell or virus for its activities and development. Replication is the process by which DNA copies itse ...
File - Dr Hayley Siddons
File - Dr Hayley Siddons

... The allele for normal colour vision is dominant A female who has an allele for colour-deficient vision on one X chromosome and an allele for normal colour vision on the other, will have normal colour vision. However she is called a carrier because she carries the affected gene for colour ...
DNA to Protein Worksheet
DNA to Protein Worksheet

... Second, transcribe the DNA sequence into an mRNA sequence and record in the second row of each table. Each box should have three (3) bases (one codon). Third, indicate what the anticodon on the tRNA would be and write each sequence in the third row. Finally, use the mRNA sequence to determine what a ...
Chapter 25: Molecular Basis of Inheritance
Chapter 25: Molecular Basis of Inheritance

DNA Strand 2
DNA Strand 2

... These nucleotides –G,C,A,T- are code for the synthesis (putting together) of proteins and proteins make traits. The long strands of DNA have special segments called genes and these genes hold the genetic code, or the blueprints for life. DNA is used by the cell as the storehouse of information on ho ...
From DNA to Protein
From DNA to Protein

... Redundant but not Ambiguous • Redundant- multiple codons can code for the same amino acid ...
DNA Song (Row, Row, Row your Boat)
DNA Song (Row, Row, Row your Boat)

... certain traits such as blue eyes, or baldness. ...
document
document

Microbiology - Las Positas College
Microbiology - Las Positas College

... List some properties of vectors and describe their use. Outline the steps in PCR and provide an examples of its use. Describe various different ways of getting DNA into a cell. Differentiate cDNA from synthetic DNA. Explain how each of the following are used to locate a clone: antibiotic-resistance ...
Reg Bio DNA tech 2013 ppt
Reg Bio DNA tech 2013 ppt

... Bacterial enzymes are used to cut DNA molecules Recognize specific sequences of nucleotides Create “sticky ends” that can bind to complimentary chains of DNA Used to cut DNA samples for DNA fingerprinting and cloning vectors ...
Site Directed Nucleases (SDN) for targeted
Site Directed Nucleases (SDN) for targeted

... Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated proteins (CRISPR/Cas) (Gaj et al., 2013, Podevin et al., 2013). Collectively, these are often discussed under the acronym site directed nucleases (SDNs), pointing out to the general principle of the technology to use a DNA cutting e ...
dna-proteins-m
dna-proteins-m

... b. DNA is composed of either purines or pyrimidines, but not both. c. DNA molecules are arranged as a tightly coiled helix. d. DNA and proteins have the same basic structure. The amount of guanine in an organism always equals the amount of a. protein. c. adenine. b. thymine. d. cytosine. During DNA ...
9-1
9-1

... PCR makes many copies of a specific DNA sequence in a few hours. PCR is a three-step process. 1)Separating – container with all reactants is heated to separate the DNA strands 2)Binding – container is cooled; nucleotides bind to the original DNA by base pairing 3)Copying – container is heated again ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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