Noureddine Loukili Studies on UEV, a new regulator of polyubiquitination:
... The ubiquitin is a heat-stable small molecule (8.6 kDa) with 76 amino acids, that adopts a stable compact globular conformation with four strands of β-sheet and a single α-helix. Its name comes from the fact that it is abundant in all eukaryotes, and extraordinarily well conserved in creatures as di ...
... The ubiquitin is a heat-stable small molecule (8.6 kDa) with 76 amino acids, that adopts a stable compact globular conformation with four strands of β-sheet and a single α-helix. Its name comes from the fact that it is abundant in all eukaryotes, and extraordinarily well conserved in creatures as di ...
HbF inducers
... exhibiting >2% HbF baseline levels responded to treatment, while those with lower HbF levels were resistant to this treatment ● Butyrate was also assayed in β-thalassemia patients ● Na phenyl butyrate: increase Hb levels 2gr/dl(1-2.5) ● Arginine butyrate ± EPO: increase Hb levels 2.7gr/dl (1-5). It ...
... exhibiting >2% HbF baseline levels responded to treatment, while those with lower HbF levels were resistant to this treatment ● Butyrate was also assayed in β-thalassemia patients ● Na phenyl butyrate: increase Hb levels 2gr/dl(1-2.5) ● Arginine butyrate ± EPO: increase Hb levels 2.7gr/dl (1-5). It ...
The bovine papillomavirus type 4 long control region contains an
... mucosal epitheliotropic viruses HPV-16 and -18 are the most common viruses associated with cervical carcinoma and one of the mechanisms restricting these viruses to epithelial cell types involves transcriptional control. The transforming genes of these viruses, E6 and E7, are controlled by the long ...
... mucosal epitheliotropic viruses HPV-16 and -18 are the most common viruses associated with cervical carcinoma and one of the mechanisms restricting these viruses to epithelial cell types involves transcriptional control. The transforming genes of these viruses, E6 and E7, are controlled by the long ...
Bridging structural biology and genomics: assessing protein
... and genetic protein-interaction studies. The 3D structures of RNA polymerase II, the proteasome and the Arp2/3 complex, which have 10, 14 and 7 subunits, respectively, were examined to identify stable protein–protein interaction interfaces, defined as more than 800 Å2 of contacting surface area. Thi ...
... and genetic protein-interaction studies. The 3D structures of RNA polymerase II, the proteasome and the Arp2/3 complex, which have 10, 14 and 7 subunits, respectively, were examined to identify stable protein–protein interaction interfaces, defined as more than 800 Å2 of contacting surface area. Thi ...
DISC1 on the P300 Waveform in Psychosis Effect of
... Structured interviews using the Schedule for Affective Disorders and Schizophrenia-Lifetime version23 or the Structured Clinical Interview for DSM-IV Axis I Disorders24 were completed and additional clinical information regarding the timing and nature of symptoms obtained to enable DSM-IV diagnoses ...
... Structured interviews using the Schedule for Affective Disorders and Schizophrenia-Lifetime version23 or the Structured Clinical Interview for DSM-IV Axis I Disorders24 were completed and additional clinical information regarding the timing and nature of symptoms obtained to enable DSM-IV diagnoses ...
lecture - Berkeley MCB
... regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have sequences for them • Even when a ...
... regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have sequences for them • Even when a ...
Two Waves of Nuclear Factor κB Recruitment to Target Promoters
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
The relationship between higher‑order chromatin structure and
... have reasonable models for. However, it is clear from electron microscopy studies that a lot of mammalian chromatin is packaged into levels beyond this. The few assays that we have for tertiary levels of chromatin structure are, in the main, cytological. There does seem to be a link between chromati ...
... have reasonable models for. However, it is clear from electron microscopy studies that a lot of mammalian chromatin is packaged into levels beyond this. The few assays that we have for tertiary levels of chromatin structure are, in the main, cytological. There does seem to be a link between chromati ...
Point mutations in BCL6 DNA-binding domain reveal distinct
... proteins and for nuclear targeting. However, the functional specificity of the six ZFs in these cellular roles is unknown. To characterize this domain, we mutated individually each ZF of BCL6. Our results reveal that mutation of the two N-terminal ZFs does not impair cognate DNA-binding, cellular lo ...
... proteins and for nuclear targeting. However, the functional specificity of the six ZFs in these cellular roles is unknown. To characterize this domain, we mutated individually each ZF of BCL6. Our results reveal that mutation of the two N-terminal ZFs does not impair cognate DNA-binding, cellular lo ...
THEMATICS: A simple computational predictor of enzyme function
... The theoretical titration functions for AR reveal seven residues with unusual curves: Tyr-48, Cys-298, Glu-185, Lys-21, Lys-77, Tyr-107, and Tyr-209. Fig. 1B shows the predicted mean net charge as a function of pH for five selected tyrosine residues. (Tyrosine residues were selected to show typical ...
... The theoretical titration functions for AR reveal seven residues with unusual curves: Tyr-48, Cys-298, Glu-185, Lys-21, Lys-77, Tyr-107, and Tyr-209. Fig. 1B shows the predicted mean net charge as a function of pH for five selected tyrosine residues. (Tyrosine residues were selected to show typical ...
Long Noncoding RNA as a Regulator for Transcription
... liposarcoma) inhibits histone acetyltransferase (HAT) activity of CBP and p300 (Wang et al. 2008b). The HAT inhibitor, TLS, turns out to have specific target genes, cyclin D1 and E1, and represses the expression of cyclin D1 upon binding the RNA containing the GGUG-consensus sequence (Lerga et al. 2 ...
... liposarcoma) inhibits histone acetyltransferase (HAT) activity of CBP and p300 (Wang et al. 2008b). The HAT inhibitor, TLS, turns out to have specific target genes, cyclin D1 and E1, and represses the expression of cyclin D1 upon binding the RNA containing the GGUG-consensus sequence (Lerga et al. 2 ...
A. thaliana - UC Davis Plant Sciences
... Identification of SCR specificity determinants by domain swapping Regions between Cys-3 and Cys-4; Cys-5 and Cys6 are candidate specificity determinants - predicted to be surface-exposed Generated SCR6-SCR13 chimeras by exchanging various domains Only SCR6 chimera (5-6) showed modified specif ...
... Identification of SCR specificity determinants by domain swapping Regions between Cys-3 and Cys-4; Cys-5 and Cys6 are candidate specificity determinants - predicted to be surface-exposed Generated SCR6-SCR13 chimeras by exchanging various domains Only SCR6 chimera (5-6) showed modified specif ...
Technique ChIC and ChEC: Genomic Mapping of Chromatin Proteins
... binding of antibodies and pA-MN. Hence, it is necessary to use high-quality antibodies and optimized concentrations of the added components (antisera and pA-MN) and to execute the washing steps with high stringency. One way to examine the specificity questions is by immunofluorescence microscopy usi ...
... binding of antibodies and pA-MN. Hence, it is necessary to use high-quality antibodies and optimized concentrations of the added components (antisera and pA-MN) and to execute the washing steps with high stringency. One way to examine the specificity questions is by immunofluorescence microscopy usi ...
Chromatin dynamics during cellular differentiation in the female
... important rewiring of the transcriptional program. The surrounding somatic cells play a central role in either promoting or restricting the developmental competence of the SMC and gametophyte (for comprehensive reviews, see Feng et al., 2013; Schmidt et al., 2015). The molecular pathways involved in ...
... important rewiring of the transcriptional program. The surrounding somatic cells play a central role in either promoting or restricting the developmental competence of the SMC and gametophyte (for comprehensive reviews, see Feng et al., 2013; Schmidt et al., 2015). The molecular pathways involved in ...
View as PDF
... that functions at telomeres and the silent mating-type loci, HMR and HML. We show that expression of the Nand C-terminal-encoding halves of SIR3 in trans partially complements the mating defect of the sir3 null allele, suggesting that the two domains have distinct functions. We present here a functi ...
... that functions at telomeres and the silent mating-type loci, HMR and HML. We show that expression of the Nand C-terminal-encoding halves of SIR3 in trans partially complements the mating defect of the sir3 null allele, suggesting that the two domains have distinct functions. We present here a functi ...
Full-Text PDF
... (5-methoxycarbonylmethyl(2-thio)uridine) modifications at position 34 from the anticodon loop of some tRNAs (Figure 2; [13,39]). In S. cerevisiae, the formation of mcm5 (s2 )U involves at least 15 proteins. The first reaction, consisting of the addition of the carboxymethyl group at position 5 of th ...
... (5-methoxycarbonylmethyl(2-thio)uridine) modifications at position 34 from the anticodon loop of some tRNAs (Figure 2; [13,39]). In S. cerevisiae, the formation of mcm5 (s2 )U involves at least 15 proteins. The first reaction, consisting of the addition of the carboxymethyl group at position 5 of th ...
Structure and functions of lampbrush chromosomes
... compounds bound with LBC loops. The transcriptionally active loops represent 5-10% of DNA. The remainder is inactive chromatin compacted in the chromomeres. The result of transcription is visible under an electron microscope as a ribonucleoproteinic mantle. The mantle tends to be asymmetrical, corre ...
... compounds bound with LBC loops. The transcriptionally active loops represent 5-10% of DNA. The remainder is inactive chromatin compacted in the chromomeres. The result of transcription is visible under an electron microscope as a ribonucleoproteinic mantle. The mantle tends to be asymmetrical, corre ...
Structure-Function Analysis of the Conserved Histone Chaperone
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
Development of Zinc Finger Domains for Recognition of the 5
... reactions contained 15 kcpm 32P-end-labeled ERBB-2 promoter fragment and 5 mM dithiothreitol, and the protein concentration was varied from 0.1 to 100 nM. Reactions were incubated at 4 °C for 12–18 h. Digestion of DNA was performed using DNase I (Roche Diagnostics) as has been described (34). Sample ...
... reactions contained 15 kcpm 32P-end-labeled ERBB-2 promoter fragment and 5 mM dithiothreitol, and the protein concentration was varied from 0.1 to 100 nM. Reactions were incubated at 4 °C for 12–18 h. Digestion of DNA was performed using DNase I (Roche Diagnostics) as has been described (34). Sample ...
Transgenerational epigenetics in the germline cycle
... of such expansion, it is probably useful to consider two main mechanistic components that have to exist in any epigenetic process: 1) There is an initiating event that affects activity or structural state at a locus or loci, and 2) there are subsequent processes that maintain the altered activity or ...
... of such expansion, it is probably useful to consider two main mechanistic components that have to exist in any epigenetic process: 1) There is an initiating event that affects activity or structural state at a locus or loci, and 2) there are subsequent processes that maintain the altered activity or ...
Nat. Struct. Biol. 8, 192-194.
... Much of the work on chromatin insulators has centered on the characterization of associated proteins with the goal of explaining how these sequences affect enhancer function, however, progress in the field has been hampered by the lack of understanding of how enhancers activate transcription. Enhanc ...
... Much of the work on chromatin insulators has centered on the characterization of associated proteins with the goal of explaining how these sequences affect enhancer function, however, progress in the field has been hampered by the lack of understanding of how enhancers activate transcription. Enhanc ...
Chromatin DNA Methylayion
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
functional analysis of chromatin assembly genes in tetrahymena
... First and foremost, I express my extreme gratitude and appreciation toward Dr. Jeffrey Fillingham for providing me with an exceptional opportunity to grow and gain knowledge in the field of molecular biology under his supportive guidance. I am also extremely thankful to Dr.Jyoti Garg who provided me ...
... First and foremost, I express my extreme gratitude and appreciation toward Dr. Jeffrey Fillingham for providing me with an exceptional opportunity to grow and gain knowledge in the field of molecular biology under his supportive guidance. I am also extremely thankful to Dr.Jyoti Garg who provided me ...
Yeast whole-genome analysis of conserved regulatory motifs
... – Determine type of function: especially outside protein-coding – Reveal relevant cell type of activity – Link to upstream regulators and downstream target genes ...
... – Determine type of function: especially outside protein-coding – Reveal relevant cell type of activity – Link to upstream regulators and downstream target genes ...
Histone acetyltransferase
Histone acetyltransferases (HATs) are enzymes that acetylate conserved lysine amino acids on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyllysine. DNA is wrapped around histones, and, by transferring an acetyl group to the histones, genes can be turned on and off. In general, histone acetylation increases gene expression.In general, histone acetylation is linked to transcriptional activation and associated with euchromatin. When it was first discovered, it was thought that acetylation of lysine neutralizes the positive charge normally present, thus reducing affinity between histone and (negatively charged) DNA, which renders DNA more accessible to transcription factors. Research has emerged, since, to show that lysine acetylation and other posttranslational modifications of histones generate binding sites for specific protein–protein interaction domains, such as the acetyllysine-binding bromodomain. Histone acetyltransferases can also acetylate non-histone proteins, such as nuclear receptors and other transcription factors to facilitate gene expression.