Chromatin-Remodeling and Memory Factors
... promoters and regulatory regions that affect chromatin structure and transcription. Thus, promoterspecific modulation in core histone acetylation concomitant with changes in transcription activity has been demonstrated for several yeast and human genes and in one plant gene (e.g. Kuo et al., 1998; K ...
... promoters and regulatory regions that affect chromatin structure and transcription. Thus, promoterspecific modulation in core histone acetylation concomitant with changes in transcription activity has been demonstrated for several yeast and human genes and in one plant gene (e.g. Kuo et al., 1998; K ...
PDF
... by the unregulated expansion of renal epithelial cells. A leading cause of endstage renal failure, autosomal dominant PKD is caused by mutations in PKD1 and PKD2, which encode polycystin 1 and 2. These large transmembrane proteins form a cation channel complex that is involved in mechanosensationtri ...
... by the unregulated expansion of renal epithelial cells. A leading cause of endstage renal failure, autosomal dominant PKD is caused by mutations in PKD1 and PKD2, which encode polycystin 1 and 2. These large transmembrane proteins form a cation channel complex that is involved in mechanosensationtri ...
The diagram below shows the arrangement of chromatin (thick black
... The diagram below shows the arrangement of chromatin (thick black strands) around histones on a chromosome in the nucleus of a eukaryotic cell. ...
... The diagram below shows the arrangement of chromatin (thick black strands) around histones on a chromosome in the nucleus of a eukaryotic cell. ...
Lecture 15 Genetic Regulation
... Metal ions are not passive components of biological systems as once thought. Recent studies have shown that Fe, Cu, or Zn are capable of regulating the expression of genes that code for that metal’s storage or transport proteins ...
... Metal ions are not passive components of biological systems as once thought. Recent studies have shown that Fe, Cu, or Zn are capable of regulating the expression of genes that code for that metal’s storage or transport proteins ...
Eukaryotic gene expression
... binding and activation are distinct, their domains may be separated on the level of DNA – By taking a domain for DNA binding and adding it to a domain for activation, a new protein may be engineered – This binds the DNA sequence specified by one gene, and responds to the signals of another – Such ex ...
... binding and activation are distinct, their domains may be separated on the level of DNA – By taking a domain for DNA binding and adding it to a domain for activation, a new protein may be engineered – This binds the DNA sequence specified by one gene, and responds to the signals of another – Such ex ...
Leukaemia Section t(2;11)(q37;q23) in AML Atlas of Genetics and Cytogenetics
... transcriptional repression activity; RD2 recruits histone desacetylases HDAC1 and HDAC2; 3 plant homeodomains (cystein rich zinc finger domains, with homodimerization properties), 1 bromodomain (may bind acetylated histones), and 1 plant homeodomain; these domains may be involved in protein-protein ...
... transcriptional repression activity; RD2 recruits histone desacetylases HDAC1 and HDAC2; 3 plant homeodomains (cystein rich zinc finger domains, with homodimerization properties), 1 bromodomain (may bind acetylated histones), and 1 plant homeodomain; these domains may be involved in protein-protein ...
The Nucleus: DNA, Chromatin And Chromosomes
... Nucleosomes can be isolated from chromatin by treatment with a hypotonic solution in an aqueous medium. ...
... Nucleosomes can be isolated from chromatin by treatment with a hypotonic solution in an aqueous medium. ...
Yeast Two Hybrid Screening: A new service at the DKFZ
... cytoskeleton regulation of organization and transcription, biogenesis DNA-dependent ...
... cytoskeleton regulation of organization and transcription, biogenesis DNA-dependent ...
The Copernican revolution of the biology
... Iris Jonkers & John T. Nature Reviews Genetics 16, 2015; Lis, Lanctôt et al. Nature Reviews Genetics 8, 2007 ...
... Iris Jonkers & John T. Nature Reviews Genetics 16, 2015; Lis, Lanctôt et al. Nature Reviews Genetics 8, 2007 ...
EXPLORE THE ISSUE BEING INVESTIGATED
... factor has attached to the DNA. By having particular shapes that bump into the regulatory proteins Pit-1 and NLI in just such a way, these domains influence what happens as the polymerase attempts to transcribe the genes directing production of the cell types. In humans, two mutations in the LHX3 ge ...
... factor has attached to the DNA. By having particular shapes that bump into the regulatory proteins Pit-1 and NLI in just such a way, these domains influence what happens as the polymerase attempts to transcribe the genes directing production of the cell types. In humans, two mutations in the LHX3 ge ...
Gene Expression
... Tissue-specific transcription factors activate transcription in specific cell types, or in response to specific signals. They bind to short DNA sequences that are near the promoter. – Used to be thought promoters were upstream from the promoter, but it is now known they can be either upstream or dow ...
... Tissue-specific transcription factors activate transcription in specific cell types, or in response to specific signals. They bind to short DNA sequences that are near the promoter. – Used to be thought promoters were upstream from the promoter, but it is now known they can be either upstream or dow ...
Zebra: web-server for bioinformatic analysis of large protein
... systematically study diverse protein superfamilies and identify the subfamily-specific positions (SSPs) – conserved only within functional subfamilies but different between them – that seem to be responsible for different substrate specificity, catalytic activity, stability, etc. [1]. It is known fr ...
... systematically study diverse protein superfamilies and identify the subfamily-specific positions (SSPs) – conserved only within functional subfamilies but different between them – that seem to be responsible for different substrate specificity, catalytic activity, stability, etc. [1]. It is known fr ...
Final lecture
... • Hemimethylated sites are converted to fully methylated sites by a maintenance methyltransferase. • TET proteins convert 5-methylcytosine to 5hydroxymethylcytosine to lead to DNA demethylation. ...
... • Hemimethylated sites are converted to fully methylated sites by a maintenance methyltransferase. • TET proteins convert 5-methylcytosine to 5hydroxymethylcytosine to lead to DNA demethylation. ...
Archaeal Transcription Initiation - IMBB
... of the eucaryal TBP and TFIIB transcription initiation factors (Bult et al., 1996). Consistent with this, these are the only archaeal transcription factors needed to direct accurate transcription initiation in vitro by archaeal RNA polymerases (Thomm, 1996). Intriguingly, despite the usual complexit ...
... of the eucaryal TBP and TFIIB transcription initiation factors (Bult et al., 1996). Consistent with this, these are the only archaeal transcription factors needed to direct accurate transcription initiation in vitro by archaeal RNA polymerases (Thomm, 1996). Intriguingly, despite the usual complexit ...
File S1 - Genes | Genomes | Genetics
... by the modENCODE project using 1kb windows. The highest degrees of correlation were ...
... by the modENCODE project using 1kb windows. The highest degrees of correlation were ...
CHAPTER 17
... core promoter of certain genes, but not next to the core promoter of most genes. Answer: The glucocorticoid receptor binds only next to genes that have a GRE by their core promoters. FIGURE 17.8 Concept check: How might nucleosome eviction affect transcription? Answer: Nucleosome eviction may allow ...
... core promoter of certain genes, but not next to the core promoter of most genes. Answer: The glucocorticoid receptor binds only next to genes that have a GRE by their core promoters. FIGURE 17.8 Concept check: How might nucleosome eviction affect transcription? Answer: Nucleosome eviction may allow ...
Epigenetics
... • genes are “turned on” or “turned off” by chemical alterations to: - DNA (e.g.methylation) or RNA - histone modification (methylation, acetylation, etc.) • epigenetic changes can be heritable • if DNA (genome) is the hardware of a computer, epigenetics (epigenome) is like the “software”. ...
... • genes are “turned on” or “turned off” by chemical alterations to: - DNA (e.g.methylation) or RNA - histone modification (methylation, acetylation, etc.) • epigenetic changes can be heritable • if DNA (genome) is the hardware of a computer, epigenetics (epigenome) is like the “software”. ...
Guest lecture 3130 2015 - Scheid Signalling Lab @ York University
... • Sumoylation is the addition of one or more copies of the 101-amino acid polypeptide SUMO (Small Ubiquitin-Related Modifier) to lysine residues on a protein • Process is similar to ubiquitylation • Results quite different – sumoylated activators are targeted to a specific nuclear compartment that k ...
... • Sumoylation is the addition of one or more copies of the 101-amino acid polypeptide SUMO (Small Ubiquitin-Related Modifier) to lysine residues on a protein • Process is similar to ubiquitylation • Results quite different – sumoylated activators are targeted to a specific nuclear compartment that k ...
Genome Organization
... – Abundant – Histone protein sequence is highly conserved among eukaryotes—conserved function – Provide the first level of packaging for the chromosome; compact the chromosome by a factor of approximately 7 – DNA is wound around histone proteins to produce nucleosomes; stretch of unwound DNA between ...
... – Abundant – Histone protein sequence is highly conserved among eukaryotes—conserved function – Provide the first level of packaging for the chromosome; compact the chromosome by a factor of approximately 7 – DNA is wound around histone proteins to produce nucleosomes; stretch of unwound DNA between ...
Procaryotic chromosome
... essential for DNA packing to nucleoid HU –small, dimeric & basic, non-specific binding H-NS –monomeric, neutral Specific DNA binding proteins RNA polymerases etc. ...
... essential for DNA packing to nucleoid HU –small, dimeric & basic, non-specific binding H-NS –monomeric, neutral Specific DNA binding proteins RNA polymerases etc. ...
Ph.D. THESIS Analysis of the chromatin structure of MDR1 gene in
... that can enter the cell through diffusion. The drug efflux is generally mediated by the increased activity of a transporter, encoded by ABCB1/MDR1 (multidrug resistance 1) gene, a member of the family of ABC (ATP binding cassette) transporters. Clinical studies have described that MDR1 gene is often ...
... that can enter the cell through diffusion. The drug efflux is generally mediated by the increased activity of a transporter, encoded by ABCB1/MDR1 (multidrug resistance 1) gene, a member of the family of ABC (ATP binding cassette) transporters. Clinical studies have described that MDR1 gene is often ...
Modular Structure of Transcription Factors: Implications for Gene
... Another unexpected feature of many transcription factor domains, especially eukaryotic ones, is that they sometimes show only a modest degree of specificity and affinity in their interactions with ligands. For example, )L repressor binds to specific operator sequences with affinities as high as lo-l ...
... Another unexpected feature of many transcription factor domains, especially eukaryotic ones, is that they sometimes show only a modest degree of specificity and affinity in their interactions with ligands. For example, )L repressor binds to specific operator sequences with affinities as high as lo-l ...
Gene Section THRAP3 (thyroid hormone receptor associated protein 3)
... Moreover, TRAP150 was also reported to interact with the domains that provide links between transcription and splicing, such as the WW domains of pinin and the ...
... Moreover, TRAP150 was also reported to interact with the domains that provide links between transcription and splicing, such as the WW domains of pinin and the ...
Expressing_CENH3_Orthologs
... Faithful chromosome segregation is mediated by the centromere and the kinetochore (1). The main protein that recruits other kinetochore proteins and assembles a functional centromere is the centromere-specific histone H3 (CENH3) which replaces the canonical histone H3 in centromeric chromatin (2). C ...
... Faithful chromosome segregation is mediated by the centromere and the kinetochore (1). The main protein that recruits other kinetochore proteins and assembles a functional centromere is the centromere-specific histone H3 (CENH3) which replaces the canonical histone H3 in centromeric chromatin (2). C ...
Histone acetyltransferase
Histone acetyltransferases (HATs) are enzymes that acetylate conserved lysine amino acids on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyllysine. DNA is wrapped around histones, and, by transferring an acetyl group to the histones, genes can be turned on and off. In general, histone acetylation increases gene expression.In general, histone acetylation is linked to transcriptional activation and associated with euchromatin. When it was first discovered, it was thought that acetylation of lysine neutralizes the positive charge normally present, thus reducing affinity between histone and (negatively charged) DNA, which renders DNA more accessible to transcription factors. Research has emerged, since, to show that lysine acetylation and other posttranslational modifications of histones generate binding sites for specific protein–protein interaction domains, such as the acetyllysine-binding bromodomain. Histone acetyltransferases can also acetylate non-histone proteins, such as nuclear receptors and other transcription factors to facilitate gene expression.