phenotype describes a new mutation affecting
... Altered expression of several ABA responsive genes is observed in different vp mutants: in cultured embryos of class II mutants there is usually a reduced basal level of the transcript, but gene expression can be induced by treating embryos with ABA (Butler and Cuming, 1993; Paiva and Kriz, 1994; Pl ...
... Altered expression of several ABA responsive genes is observed in different vp mutants: in cultured embryos of class II mutants there is usually a reduced basal level of the transcript, but gene expression can be induced by treating embryos with ABA (Butler and Cuming, 1993; Paiva and Kriz, 1994; Pl ...
protein 2 gene: study of a cohort of Israeli patients - MRC
... 453CRT (pD151E), resulting in a change of a conserved residue at the methyl-binding domain, and a rare GT deletion of intron 1 donor splice site are reported. It is shown that various MECP2 mutations had distinct effects on MECP2 expression levels in peripheral blood. The most significant (p,0.001) ...
... 453CRT (pD151E), resulting in a change of a conserved residue at the methyl-binding domain, and a rare GT deletion of intron 1 donor splice site are reported. It is shown that various MECP2 mutations had distinct effects on MECP2 expression levels in peripheral blood. The most significant (p,0.001) ...
Regulation of meiotic progression by the meiosis
... responses cause genomic instability, leading to tumor development (reviewed by Weinert, 1998; Lowndes and Murguia, 2000; Melo and Toczyski, 2002). Meiosis is a specialized type of cell division that generates haploid gametes from diploid parental cells because a single round of DNA replication is fo ...
... responses cause genomic instability, leading to tumor development (reviewed by Weinert, 1998; Lowndes and Murguia, 2000; Melo and Toczyski, 2002). Meiosis is a specialized type of cell division that generates haploid gametes from diploid parental cells because a single round of DNA replication is fo ...
Distalless and dachshund pattern both plesiomorphic
... conferring proximal (hth and exd), medial (dac), and distal (Dll) identities (Sunkel and Whittle 1987; Cohen and Jürgens 1989; Mardon et al. 1994; González‐Crespo and Morata 1996; Lecuit and Cohen 1997; Rieckhof et al. 1997; Abu‐Shaar and Mann 1998; Casares and Mann 1998; Abu‐Shaar et al. 1999; Wu a ...
... conferring proximal (hth and exd), medial (dac), and distal (Dll) identities (Sunkel and Whittle 1987; Cohen and Jürgens 1989; Mardon et al. 1994; González‐Crespo and Morata 1996; Lecuit and Cohen 1997; Rieckhof et al. 1997; Abu‐Shaar and Mann 1998; Casares and Mann 1998; Abu‐Shaar et al. 1999; Wu a ...
A role for wingless in the segmental gradient of Drosophila?
... metric; in both thorax and abdomen, the denticles of each segment are oriented towards a central mirror plane. The mirror plane runs through the middle of the belt, irrespective of the belt size (compare, for example, T1 with T2 in Fig. 2C). Furthermore, each belt is symmetrical with respect to the ...
... metric; in both thorax and abdomen, the denticles of each segment are oriented towards a central mirror plane. The mirror plane runs through the middle of the belt, irrespective of the belt size (compare, for example, T1 with T2 in Fig. 2C). Furthermore, each belt is symmetrical with respect to the ...
Mycoplasma genitalium: a brief review
... in the M. genitalium genome correspond to certain sequences of M. pneumoniae’s genome.14 The small genome of M. genitalium gives a good indication of the minimal set of genes needed to sustain bacterial life. The minimum set of genes, also called essential genes, in both prokaryotes and eukaryotes, ...
... in the M. genitalium genome correspond to certain sequences of M. pneumoniae’s genome.14 The small genome of M. genitalium gives a good indication of the minimal set of genes needed to sustain bacterial life. The minimum set of genes, also called essential genes, in both prokaryotes and eukaryotes, ...
PH4 of Petunia Is an R2R3 MYB Protein That Activates
... AN1 and AN11 are required for transcriptional activation of a subset of structural anthocyanin genes, encoding the enzymes of the pathway, in all pigmented tissues (Quattrocchio et al., 1993) and encode a basic-helix-loop-helix (BHLH) transcription factor and a WD40 protein, respectively (de Vetten ...
... AN1 and AN11 are required for transcriptional activation of a subset of structural anthocyanin genes, encoding the enzymes of the pathway, in all pigmented tissues (Quattrocchio et al., 1993) and encode a basic-helix-loop-helix (BHLH) transcription factor and a WD40 protein, respectively (de Vetten ...
The Choline/Ethanolamine Kinase Family in
... Arabidopsis Information Resource, www.arabidopsis.org). We therefore excluded this gene from our study. The CEKs encode ...
... Arabidopsis Information Resource, www.arabidopsis.org). We therefore excluded this gene from our study. The CEKs encode ...
PDF reprint - Stanford Medicine
... Drosophila genomic DNA and obtained six DNA fragments that satisfied genetic criteria in yeast for Tin binding sites (Figure 1B). Most of the genomic DNA fragments were isolated multiple times. Sequence analysis confirmed the presence of core recognition sites for NK class homeodomains in all of the ...
... Drosophila genomic DNA and obtained six DNA fragments that satisfied genetic criteria in yeast for Tin binding sites (Figure 1B). Most of the genomic DNA fragments were isolated multiple times. Sequence analysis confirmed the presence of core recognition sites for NK class homeodomains in all of the ...
PDF
... and Collagen type II at high levels, and stained positively with Alcian Blue, markers that are all absent from the wild-type vp. All three markers were also occasionally seen in the malformed ap and pp of lep, uki, igu and ptc1+/–; lep–/– embryos (data not shown). Expression of otx1 in the dysmorphi ...
... and Collagen type II at high levels, and stained positively with Alcian Blue, markers that are all absent from the wild-type vp. All three markers were also occasionally seen in the malformed ap and pp of lep, uki, igu and ptc1+/–; lep–/– embryos (data not shown). Expression of otx1 in the dysmorphi ...
Resveratrol Opportunism: What Is the Science Behind the Claims?
... benefits. It is well known that many genes are influenced positively in vitro by both resveratrol and methylated resveratrol; therefore, it is premature to conclude that one form is more beneficial than the other.13 It is likely that resveratrol and its methylated derivatives such as pterostilbene h ...
... benefits. It is well known that many genes are influenced positively in vitro by both resveratrol and methylated resveratrol; therefore, it is premature to conclude that one form is more beneficial than the other.13 It is likely that resveratrol and its methylated derivatives such as pterostilbene h ...
Isolation and characterization of an RNA that binds with high affinity
... results demonstrate that factors that are important in the transcription process can bind to TAR RNA and, therefore, that the TAR decoy can inhibit transcription in vitro. In analogous experiments based on the LTR promoter, we observed similar inhibition of transcription (data not shown). The effect ...
... results demonstrate that factors that are important in the transcription process can bind to TAR RNA and, therefore, that the TAR decoy can inhibit transcription in vitro. In analogous experiments based on the LTR promoter, we observed similar inhibition of transcription (data not shown). The effect ...
Identification of Functional Domains in the Cohesin Loader Subunit
... was prepared using a transposon insertion kit, in which a 57 bp DNA fragment was randomly inserted into a CEN URA3 plasmid harboring the SCC4 coding sequence under the control of the GAL promoter (Materials and Methods and Figure S1). During preparation of the library, we noticed residual kanamycin ...
... was prepared using a transposon insertion kit, in which a 57 bp DNA fragment was randomly inserted into a CEN URA3 plasmid harboring the SCC4 coding sequence under the control of the GAL promoter (Materials and Methods and Figure S1). During preparation of the library, we noticed residual kanamycin ...
Regulation of limb bud initiation and limbtype
... and although npnt is ubiquitously expressed during frog development at the time forelimb outgrowth is initiated, transcripts are enriched in pronephros. The correlation with pronephros is potentially significant since extirpation experiments in the chick have implicated nephrogenic mesoderm in foreli ...
... and although npnt is ubiquitously expressed during frog development at the time forelimb outgrowth is initiated, transcripts are enriched in pronephros. The correlation with pronephros is potentially significant since extirpation experiments in the chick have implicated nephrogenic mesoderm in foreli ...
neurotic, a novel maternal neurogenic gene, encodes an O
... serine or threonine residues in the consensus sequence CXXGGS/TC (X is any amino acids) between the second and third conserved cysteines of EGF repeats (Harris and Spellman, 1993; Wang et al., 2001). Notch and its ligands, Delta and Serrate, have such sequences in their extracellular EGF repeats, an ...
... serine or threonine residues in the consensus sequence CXXGGS/TC (X is any amino acids) between the second and third conserved cysteines of EGF repeats (Harris and Spellman, 1993; Wang et al., 2001). Notch and its ligands, Delta and Serrate, have such sequences in their extracellular EGF repeats, an ...
Developing a Bioassay for Triclosan Toxicity Abstract
... performed on RNA from embryos treated with control, solvent control and TCS treated embryos. The gene expression of actin and GAPDH were used as controls to normalize the levels of the target genes. The expression of gli2a did not change with exposure to TCS (Fig. 3). In contrast, there was a slight ...
... performed on RNA from embryos treated with control, solvent control and TCS treated embryos. The gene expression of actin and GAPDH were used as controls to normalize the levels of the target genes. The expression of gli2a did not change with exposure to TCS (Fig. 3). In contrast, there was a slight ...
A novel role for the floral homeotic gene APETALA2
... margins and replum tissues (supplementary material Fig. S1J,K), suggesting that AP2 may function during valve margin and replum development. The reporter activity gradually diminished as fruit growth proceeded (data not shown). ...
... margins and replum tissues (supplementary material Fig. S1J,K), suggesting that AP2 may function during valve margin and replum development. The reporter activity gradually diminished as fruit growth proceeded (data not shown). ...
Virp1 Is a Host Protein with a Major Role in Potato - IMBB
... Viroids are small, circular, single-stranded RNA molecules that, while not coding for any protein, cause several plant diseases. Viroids rely for their infectious cycle on host proteins, most of which are likely to be involved in endogenous RNA-mediated phenomena. Therefore, characterization of host ...
... Viroids are small, circular, single-stranded RNA molecules that, while not coding for any protein, cause several plant diseases. Viroids rely for their infectious cycle on host proteins, most of which are likely to be involved in endogenous RNA-mediated phenomena. Therefore, characterization of host ...
Requirement for chitin biosynthesis in epithelial tube morphogenesis. Proc. Natl. Acad. Sci. USA 102: 17014-17019. pdf
... Portions of the tube dilated poorly, whereas other regions expanded too much (Fig. 1Z). As expansion proceeded, the dorsal trunk became progressively more misshapen, with constricted and distended regions all along its length. Expression of the 2A12 and AS55 luminal markers, which normally begin to ...
... Portions of the tube dilated poorly, whereas other regions expanded too much (Fig. 1Z). As expansion proceeded, the dorsal trunk became progressively more misshapen, with constricted and distended regions all along its length. Expression of the 2A12 and AS55 luminal markers, which normally begin to ...
Cytokinin signaling - Biology Department | UNC Chapel Hill
... displays reduced sensitivity to exogenous cytokinin in root elongation assays. The quintuple ahp1 ahp2 ahp3 ahp4 ahp5 mutant is severely impaired in the induction of cytokinin primary response genes and shows developmental defects. Together, these findings indicate that the AHPs are positive, redund ...
... displays reduced sensitivity to exogenous cytokinin in root elongation assays. The quintuple ahp1 ahp2 ahp3 ahp4 ahp5 mutant is severely impaired in the induction of cytokinin primary response genes and shows developmental defects. Together, these findings indicate that the AHPs are positive, redund ...
Mir-92 microRNA precursor family
The miR-92 microRNAs are short single stranded non-protein coding RNA fragments initially discovered incorporated into an RNP complex with a proposed role of processing RNA molecules and further RNP assembly. Mir-92 has been mapped to the human genome as part of a larger cluster at chromosome 13q31.3, where it is 22 nucleotides in length but exists in the genome as part of a longer precursor sequence. There is an exact replica of the mir-92 precursor on the X chromosome. MicroRNAs are endogenous triggers of the RNAi pathway which involves several ribonucleic proteins (RNPs) dedicated to repressing mRNA molecules via translation inhibition and/or induction of mRNA cleavage. miRNAs are themselves matured from their long RNA precursors by ribonucleic proteins as part of a 2 step biogenesis mechanism involving RNA polymerase 2.Most miRNAs are grouped into clusters in the human genome or within families that share functions, expression profiles, promotors, or are incorporated into the same ribonucleic protein. The purpose of having a variety of miRNAs in a single piece of RNA processing machinery is to act as complementary strands to the recognition elements of a variety of target RNA molecules.The recognition elements of target mRNAs are typically within the 3' untranslated regions and with 678 human miRNAs and 472 mouse miRNAs confidently identified so far (miRBASE) there are extensive efforts taking place using bioinformatics tools to scan genomes for potential recognition elements for families of miRNAs in order to identify potential target genes.Mir-92 is no exception and currently identified gene targets have been among those involved in cell cycle regulation and cell signalling, and thus necessary during all stages of mammalian development and essential for the proliferation of cells. miRNAs can be oncogenes or tumor suppressor genes depending on their targets while mir-92 has been implicated as the former in leukaemia forms AML and ALL, Hepatocellular carcinoma (HCC) and several other cancers.The search for noninvasive tools for diagnosis and management of cancer is extremely important for reducing the worldwide health burden of cancer. miRNAs show potential as biomarkers and can even be found circulating in the serum. Some circulating miRNAs are specific to tumour patients, while miR-92 on the other-hand is present in healthy individuals in the serum, but levels are variable and appear to change in response to the onset of some cancers.