AutoDock 4 and AutoDock Vina
... Docking is frequently used to predict the binding orientation of small molecule drug candidates to their protein targets in order to in turn predict the affinity and activity of the small molecule. Hence docking plays an important role in the rational design of drugs, for example, hit identification ...
... Docking is frequently used to predict the binding orientation of small molecule drug candidates to their protein targets in order to in turn predict the affinity and activity of the small molecule. Hence docking plays an important role in the rational design of drugs, for example, hit identification ...
Q. No. 1. How can RNA be distinguished from DNA?
... Q. No.12. What is sedimentation coefficient? Ans. Sedimentation coefficient is the time taken by any solid particle to settle or to sediment in the process of centrifugation. Q.No.13. Write down the chemical composition of ribosomes Ans. Ribosomes are tiny particles, about 200 A. It is composed of b ...
... Q. No.12. What is sedimentation coefficient? Ans. Sedimentation coefficient is the time taken by any solid particle to settle or to sediment in the process of centrifugation. Q.No.13. Write down the chemical composition of ribosomes Ans. Ribosomes are tiny particles, about 200 A. It is composed of b ...
TAT Protein
... particular to the treatment of HIV/AIDS infection. The patent relates specifically to the discovery that certain peptides can target one of the HIV-1 proteins, Tat, and block its function. This discovery can lead to the development of innovative compounds blocking HIV-1 replication and HIV disease p ...
... particular to the treatment of HIV/AIDS infection. The patent relates specifically to the discovery that certain peptides can target one of the HIV-1 proteins, Tat, and block its function. This discovery can lead to the development of innovative compounds blocking HIV-1 replication and HIV disease p ...
Quant-iT™ Assay Kits for microplate
... of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double the amount shown. The inset shows the sensitivity of the assay for DNA. B The Quant-iT™ RNA Assay Kit has a linear detection range of 5–100 ng and is selec ...
... of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double the amount shown. The inset shows the sensitivity of the assay for DNA. B The Quant-iT™ RNA Assay Kit has a linear detection range of 5–100 ng and is selec ...
Immunochemical methods
... than mice or rabbits, making it more easy to obtain an immune response towards well conserved antigens. A single chicken can produce high amounts of antibody, up to 3 grams of IgY per month, which is 10-20 times the amount of a rabbit Antibodies are present in the yolk of the egg. Compared to rabbit ...
... than mice or rabbits, making it more easy to obtain an immune response towards well conserved antigens. A single chicken can produce high amounts of antibody, up to 3 grams of IgY per month, which is 10-20 times the amount of a rabbit Antibodies are present in the yolk of the egg. Compared to rabbit ...
Supplement Material…
... Wild-type ComA, wild-type His-ComA, and the His-ComA alanine-substituted mutants were overexpressed in E. coli strain BL21(DE3)pLysS grown in LB media supplemented with 15 g/ml kanamycin, 35 g/ml chloramphenicol, and 0.2% (w/v) Lglucose at 37 C to an OD600 of 0.800. Protein expression was induced ...
... Wild-type ComA, wild-type His-ComA, and the His-ComA alanine-substituted mutants were overexpressed in E. coli strain BL21(DE3)pLysS grown in LB media supplemented with 15 g/ml kanamycin, 35 g/ml chloramphenicol, and 0.2% (w/v) Lglucose at 37 C to an OD600 of 0.800. Protein expression was induced ...
The Cell Membrane
... The detailed structure of an animal cell’s plasma membrane, in cross section ...
... The detailed structure of an animal cell’s plasma membrane, in cross section ...
B1 - BBS Biology Revision
... B2.5 Proteins – functions and uses Use skills, knowledge and understanding to: evaluate the advantages and disadvantages of using enzymes in the home and in industry. You will need to know: a) Protein molecules are made up of long chains of amino acids. These long chains are folded to produce a spec ...
... B2.5 Proteins – functions and uses Use skills, knowledge and understanding to: evaluate the advantages and disadvantages of using enzymes in the home and in industry. You will need to know: a) Protein molecules are made up of long chains of amino acids. These long chains are folded to produce a spec ...
Document
... • Create a new SPARQL query or change an existing one is usually easier than create or rewrite some scripts. • We use named graphs (identified subsets of RDF statements) to separate results coming from different systems or different experiments and gold-standard data: results from different experime ...
... • Create a new SPARQL query or change an existing one is usually easier than create or rewrite some scripts. • We use named graphs (identified subsets of RDF statements) to separate results coming from different systems or different experiments and gold-standard data: results from different experime ...
From DNA to Protein
... • Met is at the start of every protein until post-translational modification takes place Eukaryotic vs Procaryotic – Figure 7-12 • Procaryotic – No CAP; have specific ribosome binding site upstream of AUG – Polycistronic – multiple proteins from same mRNA • Eucaryotic – Monocistronic – one polypepti ...
... • Met is at the start of every protein until post-translational modification takes place Eukaryotic vs Procaryotic – Figure 7-12 • Procaryotic – No CAP; have specific ribosome binding site upstream of AUG – Polycistronic – multiple proteins from same mRNA • Eucaryotic – Monocistronic – one polypepti ...
Translation - St. Robert CHS
... by the ribosomes • AUG ensures that the correct reading frame is used by the ...
... by the ribosomes • AUG ensures that the correct reading frame is used by the ...
DNA transcription
... reached. For visualisation you can watch video at: https://www.youtube.com/watch?v=gG7uCskUOrA. After a polypeptide chain is synthesized, it may undergo additional processes. For example, it may assume a folded shape due to interactions among its amino acids. It may also bind with other polypeptides ...
... reached. For visualisation you can watch video at: https://www.youtube.com/watch?v=gG7uCskUOrA. After a polypeptide chain is synthesized, it may undergo additional processes. For example, it may assume a folded shape due to interactions among its amino acids. It may also bind with other polypeptides ...
BOULANT Steeve PhD
... Targett-Adams P, Hope G, Boulant S and McLauchlan J. J Biol Chem (IF 7.7). 2008. Visualization of double-stranded RNA in cells supporting hepatitis C virus RNA replication. Targett-Adams P, Boulant S and McLauchlan J. J Virol.(IF 5.3) 2008. Transcriptional slippage at a rare adenine cluster in the c ...
... Targett-Adams P, Hope G, Boulant S and McLauchlan J. J Biol Chem (IF 7.7). 2008. Visualization of double-stranded RNA in cells supporting hepatitis C virus RNA replication. Targett-Adams P, Boulant S and McLauchlan J. J Virol.(IF 5.3) 2008. Transcriptional slippage at a rare adenine cluster in the c ...
PHL 424 1st SF
... Consequently, when infants are given dosages above 50 mg/kg/d, the drug may accumulate, resulting in a serious illness termed gray baby syndrome, with vomiting, refusal to suck, passage of loose, green stools, cyanosis, ashen-gray color, flaccidity, hypothermia, shock and collapse Death occurs in ab ...
... Consequently, when infants are given dosages above 50 mg/kg/d, the drug may accumulate, resulting in a serious illness termed gray baby syndrome, with vomiting, refusal to suck, passage of loose, green stools, cyanosis, ashen-gray color, flaccidity, hypothermia, shock and collapse Death occurs in ab ...
Database searching
... eMotif (a protein pattern database); eBLOCKs Gibbs and MEME – To infer patterns in unaligned sequences – Gibbs program starts with a fixed pattern length of W and a random set of locations of the pattern in given input sequences (i.e., the initial pattern is random); and then one sequence is sel ...
... eMotif (a protein pattern database); eBLOCKs Gibbs and MEME – To infer patterns in unaligned sequences – Gibbs program starts with a fixed pattern length of W and a random set of locations of the pattern in given input sequences (i.e., the initial pattern is random); and then one sequence is sel ...
ETHANOL CO-PRODUCTS 101
... – Grow-Finish Diets – 200-300#/ton – Limitations • Amino Acid Content – Lysine • Fiber – Grow/Finish ...
... – Grow-Finish Diets – 200-300#/ton – Limitations • Amino Acid Content – Lysine • Fiber – Grow/Finish ...
Teaching Notes
... Q1. In PDB entry 1hla, how many polymer chains are there? What are they? A1: There are 3 protein chains - HLA, beta-2 microglobulin and the antigen peptide Q2. Where are the Cys residues located? Comment about how they are contributing to the stability of the structure. A2: A single S-S bond stabili ...
... Q1. In PDB entry 1hla, how many polymer chains are there? What are they? A1: There are 3 protein chains - HLA, beta-2 microglobulin and the antigen peptide Q2. Where are the Cys residues located? Comment about how they are contributing to the stability of the structure. A2: A single S-S bond stabili ...
Review Structural glycobiology: A game of snakes and ladders
... Thus far, only rarely has the design of carbohydrate-based therapeutic agents made extensive use of 3D structural information, reflecting in part the difficulties of determining carbohydrate conformation, as well as a paucity of structural data for many carbohydrate—protein complexes. To help revers ...
... Thus far, only rarely has the design of carbohydrate-based therapeutic agents made extensive use of 3D structural information, reflecting in part the difficulties of determining carbohydrate conformation, as well as a paucity of structural data for many carbohydrate—protein complexes. To help revers ...
Model Description Sheet
... intestine and a 50% chance of developing cancer by the age of 50. When cell energy, ATP, is low LKB1 will be activated. Active LKB1 regulates the activity of adenosine monophosphateactivated protein kinase (AMPK). LKB1 directly activates AMPK by adding a phosphate group to Thr-172. AMPK activity inc ...
... intestine and a 50% chance of developing cancer by the age of 50. When cell energy, ATP, is low LKB1 will be activated. Active LKB1 regulates the activity of adenosine monophosphateactivated protein kinase (AMPK). LKB1 directly activates AMPK by adding a phosphate group to Thr-172. AMPK activity inc ...
i principi di base - Structural Biology
... The pK of the carboxylic group in the single-alanine is 2.3 and of the amminic group group is 9.6, for the dipeptide the pK of the carboxylic group is 3.1 and of the amminic group becomes 8.3, for the tripeptide 3.4 and 8.0 while for the tetrapeptide there is no change and the pK values are yet 3.4 ...
... The pK of the carboxylic group in the single-alanine is 2.3 and of the amminic group group is 9.6, for the dipeptide the pK of the carboxylic group is 3.1 and of the amminic group becomes 8.3, for the tripeptide 3.4 and 8.0 while for the tetrapeptide there is no change and the pK values are yet 3.4 ...
mRNA and protein abundance for glutathione-S
... mRNPs and mRNAs engaged in pre-translational complexes (fractions 1–10; lightblue area, designated as ‘free’), and those containing the polysome-bound mRNAs (fractions 11–20; dark-blue area, labelled ‘bound’). Hybridization with probes specific for the mRNAs encoding p38MAPK (red), GADD153 (green) a ...
... mRNPs and mRNAs engaged in pre-translational complexes (fractions 1–10; lightblue area, designated as ‘free’), and those containing the polysome-bound mRNAs (fractions 11–20; dark-blue area, labelled ‘bound’). Hybridization with probes specific for the mRNAs encoding p38MAPK (red), GADD153 (green) a ...
Sec14p-like proteins regulate phosphoinositide homoeostasis and
... Overexpression of SFH1–SFH4 fails to restore growth of any sects strain (other than sec14ts ) at restrictive temperatures, but SFH5 overexpression reverses the temperature-sensitive growth and secretory defects associated with each of the sec8-9ts , sec10-2ts and sec15-1ts mutations [10]. These muta ...
... Overexpression of SFH1–SFH4 fails to restore growth of any sects strain (other than sec14ts ) at restrictive temperatures, but SFH5 overexpression reverses the temperature-sensitive growth and secretory defects associated with each of the sec8-9ts , sec10-2ts and sec15-1ts mutations [10]. These muta ...
Protein adsorption
Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.