Chemistry 1- Separation
... porous gel which separates the molecules according to its size. The pores are normally small and exclude the larger solute molecules, but allows smaller molecules to enter the gel, causing them to flow through a larger volume. This causes the larger molecules to pass through the column at a faster r ...
... porous gel which separates the molecules according to its size. The pores are normally small and exclude the larger solute molecules, but allows smaller molecules to enter the gel, causing them to flow through a larger volume. This causes the larger molecules to pass through the column at a faster r ...
(Rev. 9/04), Biographical Sketch Format Page
... between Go and Gq in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for Go signaling and regulates Gq activity. Genes Dev. 13, 1780-1793. Dong, M.Q., Chase, D., Patikoglou, G.A., and Koelle, M.R. (2000). Multiple RGS proteins alter neural G protein signaling to allow C. elegans to r ...
... between Go and Gq in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for Go signaling and regulates Gq activity. Genes Dev. 13, 1780-1793. Dong, M.Q., Chase, D., Patikoglou, G.A., and Koelle, M.R. (2000). Multiple RGS proteins alter neural G protein signaling to allow C. elegans to r ...
Protein synthesis File
... Transfer RNA (tRNA) transports amino acids to the ribosome for incorporation into protein. Each tRNA is a single polynucleotide that is folded into a clover-leaf shape. Hydrogen bonds between complementary bases stabilise the shape. tRNA has: an anticodon that is complementary to the codon ...
... Transfer RNA (tRNA) transports amino acids to the ribosome for incorporation into protein. Each tRNA is a single polynucleotide that is folded into a clover-leaf shape. Hydrogen bonds between complementary bases stabilise the shape. tRNA has: an anticodon that is complementary to the codon ...
SecStAnT: secondary structure analysis tool for data selection
... Biomolecules coordinates are stored in a format that is a widely used, internationally referred representation for macromolecular data, including experimental and structural information. These, however, are integrated within the coordinates file, and not of immediate use to the aim of building, e.g. ...
... Biomolecules coordinates are stored in a format that is a widely used, internationally referred representation for macromolecular data, including experimental and structural information. These, however, are integrated within the coordinates file, and not of immediate use to the aim of building, e.g. ...
Beyond Antibodies
... multispecific, two antigen combining sites with different specificity in tandem on an IgG ...
... multispecific, two antigen combining sites with different specificity in tandem on an IgG ...
Elucidation of the Genetic Code
... • This would imply that more than 50% of the time, a frame shift would result in a codon that would be a ‘nonsense’ codon. • This was contrary to experimental observations • Conclusion: the genetic code is degenerate. – More than one codon can code for each amino acid. – Each codon only codes for ...
... • This would imply that more than 50% of the time, a frame shift would result in a codon that would be a ‘nonsense’ codon. • This was contrary to experimental observations • Conclusion: the genetic code is degenerate. – More than one codon can code for each amino acid. – Each codon only codes for ...
Supporting information Highly sensitive and specific protein
... NanoPro+RCA signals were collected using HUVEC cell lysate to show identify overlapping and unique peaks representing the different proteins. Typically, phosphoprotein variants appear as unique peaks with a more acidic pI than the total protein peaks. (a) Detailed overlay graph of pERK1/2 (pink) and ...
... NanoPro+RCA signals were collected using HUVEC cell lysate to show identify overlapping and unique peaks representing the different proteins. Typically, phosphoprotein variants appear as unique peaks with a more acidic pI than the total protein peaks. (a) Detailed overlay graph of pERK1/2 (pink) and ...
BioSketch - WWU Computer Science Faculty Web Pages
... Filip Jagodzinski, Akbal-Delibas, Nurit Haspel. An Evolutionary Conservation and Rigidity Analysis Machine Learning Approach for Detecting Critical Protein Residues. Proceedings, CSBW Workshop at ACM-BCB 2013, Washington D.C. ...
... Filip Jagodzinski, Akbal-Delibas, Nurit Haspel. An Evolutionary Conservation and Rigidity Analysis Machine Learning Approach for Detecting Critical Protein Residues. Proceedings, CSBW Workshop at ACM-BCB 2013, Washington D.C. ...
Dormancy Metabolism Characteristics
... 3. Exogenous dormancy - development is delayed only because of one or more unfavorable chemical or physical conditions of the environment** *a) b) c) d) ...
... 3. Exogenous dormancy - development is delayed only because of one or more unfavorable chemical or physical conditions of the environment** *a) b) c) d) ...
7Synapse Form
... synaptic activity (e.g. tetanus or other LTPinducing stimuli) or by profound inactivity, presumably in conjunction with local secreted factors. Postsynaptic PDZ proteins are critical for the development of filopodia into mature spines representative mature mushroomshaped spine is shown). The mainten ...
... synaptic activity (e.g. tetanus or other LTPinducing stimuli) or by profound inactivity, presumably in conjunction with local secreted factors. Postsynaptic PDZ proteins are critical for the development of filopodia into mature spines representative mature mushroomshaped spine is shown). The mainten ...
Proteogenomics - The Fenyo Lab
... heavily on the quality of the protein sequence database (DB) • DBs with missing peptide sequences will fail to identify the corresponding peptides • DBs that are too large will have low sensitivity • Ideal DB is complete and small, containing all proteins in the sample and no irrelevant sequences ...
... heavily on the quality of the protein sequence database (DB) • DBs with missing peptide sequences will fail to identify the corresponding peptides • DBs that are too large will have low sensitivity • Ideal DB is complete and small, containing all proteins in the sample and no irrelevant sequences ...
Photocatalysis on TiOn Surfaces: Principles, Mechanisms, and
... exchange require orbital overlap between the interacting centers. When both processes are thermodynamically allowed, electron transfer predominates since the electron exchange process requires simultaneous overlap of two orbital pairs, whereas only one such overlap is necessary for electron t r a n ...
... exchange require orbital overlap between the interacting centers. When both processes are thermodynamically allowed, electron transfer predominates since the electron exchange process requires simultaneous overlap of two orbital pairs, whereas only one such overlap is necessary for electron t r a n ...
pH of the electrophoresis buffer
... charge-to-size-ratio of all polynucleotide: same Separation based on size changing pH of electrophoretic buffer: No affect on separation For protein: pH value
... charge-to-size-ratio of all polynucleotide: same Separation based on size changing pH of electrophoretic buffer: No affect on separation For protein: pH value
2: Enzymes
... such as column chromatography can be used to separate proteins based upon their size. Often multiple techniques are combined to isolate the pure enzyme. ...
... such as column chromatography can be used to separate proteins based upon their size. Often multiple techniques are combined to isolate the pure enzyme. ...
Human Body Poetry Booklet
... When you think of systems, think of parts of a whole, working together, with one common goal. The inputs go in, the outputs go out, and inside the system, transformations come about. ...
... When you think of systems, think of parts of a whole, working together, with one common goal. The inputs go in, the outputs go out, and inside the system, transformations come about. ...
Coarse-grained normal mode analysis in structural biology
... Overview of various methodologies and applications to the GroEL–GroES complex of EN models. The EN model (b) requires an initial input structure, typically an atomic-resolution structure such as in (a), colored according to secondary structure elements. As noted in the text, a lower-resolution struc ...
... Overview of various methodologies and applications to the GroEL–GroES complex of EN models. The EN model (b) requires an initial input structure, typically an atomic-resolution structure such as in (a), colored according to secondary structure elements. As noted in the text, a lower-resolution struc ...
Chromatography Resins for Protein Purification
... purification of biomolecules. Pall offers a line of chromatography resins ideal for protein purification applications (see Table 4.6). This broad line of chromatography products exhibits superior performance and is useful for affinity, ion exchange, size exclusion, and hydrophobic interaction chroma ...
... purification of biomolecules. Pall offers a line of chromatography resins ideal for protein purification applications (see Table 4.6). This broad line of chromatography products exhibits superior performance and is useful for affinity, ion exchange, size exclusion, and hydrophobic interaction chroma ...
5 Production of Microbial Biomass - Wiley-VCH
... be used in foodstuffs as many are pathogenic. In addition, separation is difficult because of their small size. ...
... be used in foodstuffs as many are pathogenic. In addition, separation is difficult because of their small size. ...
slides - NMRbox
... reorientation. NMR assignment is complicated, and generally requires 13C / 15N labeled protein. Y Aubin, DJ Hodgson, WB Thach, G Gingras, and S Sauvé: Monitoring Effects of Excipients, Formulation Parameters and Mutations on the High Order Structure of Filgrastim by NMR. Pharm Res., 32, 3365-3375 (2 ...
... reorientation. NMR assignment is complicated, and generally requires 13C / 15N labeled protein. Y Aubin, DJ Hodgson, WB Thach, G Gingras, and S Sauvé: Monitoring Effects of Excipients, Formulation Parameters and Mutations on the High Order Structure of Filgrastim by NMR. Pharm Res., 32, 3365-3375 (2 ...
Supplementary information - Word file (31 KB )
... This step was repeated and beads were then resuspended in 280l of TBST+0.2% BSA. 20l of this slurry (containing ~1g of GST-Smad4 protein) was mixed with 2-20l of ...
... This step was repeated and beads were then resuspended in 280l of TBST+0.2% BSA. 20l of this slurry (containing ~1g of GST-Smad4 protein) was mixed with 2-20l of ...
Transient Overexpression of Recombinant Proteins in Mammalian
... Flow cytometry was used to quantify the expression levels of AcGFP1 in adherent and suspension cells. Cells transfected with pHEK293 Ultra Expression Vector I (conditions 2 and 7) and pHEK293 Ultra Expression Vector II (conditions 3, 4, 8, and 9) displayed fluorescence intensities 5–11 times higher ...
... Flow cytometry was used to quantify the expression levels of AcGFP1 in adherent and suspension cells. Cells transfected with pHEK293 Ultra Expression Vector I (conditions 2 and 7) and pHEK293 Ultra Expression Vector II (conditions 3, 4, 8, and 9) displayed fluorescence intensities 5–11 times higher ...
ion exchange chromatography
... It is a non-denaturing technique. It can be used at all stages and scales of purification An IEX separation can be controlled by changing pH, salt concentration and/or the ion exchange media It can serve as a concentrating step. A large volume of dilute sample can be applied to a media, and the a ...
... It is a non-denaturing technique. It can be used at all stages and scales of purification An IEX separation can be controlled by changing pH, salt concentration and/or the ion exchange media It can serve as a concentrating step. A large volume of dilute sample can be applied to a media, and the a ...
ppt
... Phosphorylation is one of the most common posttranslational modifications (PTMs) for proteins and is involved in numerous biological processes. Detection of the dynamic phosphorylation state of the cellular proteome is essential for understanding the regulatory network of biological pathways. There ...
... Phosphorylation is one of the most common posttranslational modifications (PTMs) for proteins and is involved in numerous biological processes. Detection of the dynamic phosphorylation state of the cellular proteome is essential for understanding the regulatory network of biological pathways. There ...
Folding quality control in the export of proteins by the
... pathway. This facilitates protein separation, the most costly step in bioreactor processes Way to engineer proteins for transport without needed to change structure of protein ...
... pathway. This facilitates protein separation, the most costly step in bioreactor processes Way to engineer proteins for transport without needed to change structure of protein ...
Protein adsorption
Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.