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The Serum Proteins of the Rat During Development
The Serum Proteins of the Rat During Development

... An asymmetry was found in the trailing region of the gamma-globulin fraction during all stages of development, including the adult. An asymmetry very similar in appearance and position was also found by Gurvich & Karsaevskaya (1956), but only in sera from perinatal animals. These workers have design ...
Pupko_pairwise
Pupko_pairwise

... sequences are similar there are short ungapped regions of high similarity between them • The heuristic: 1. Discard irrelevant sequences 2. Perform exact local alignment only with the remaining sequences ...
types of gel - WordPress.com
types of gel - WordPress.com

... Polyacrylamide gel electrophoresis (PAGE), describes a technique widely used biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. For proteins, sodium dodecyl sulfate (SDS) is an anionic detergent applied to protein samp ...
corrected Amino acids and Protein
corrected Amino acids and Protein

... Amino acids are building blocks of proteins. More than100 amino acids have been isolated and identified but only 25 are obtained upon hydrolysis of typical proteins. All 25 except 2 are αamino acids; the two exceptions are proline and hydroxy proline, which are imino acids. Only 20 amino acids are o ...
Docking QM/MM
Docking QM/MM

... improved H-bonding interaction overcomes the small distortion cost. Experimental evidence for this type of effect can be seen from both microwave studies of common substituents such as aniline60 and from an analysis of small molecule structures reported in the CSD where hydrogen atom positions have ...
Computer Storage of Sequences
Computer Storage of Sequences

... produces and distributes the PIR-International Protein Sequence Database (PSD) .  Comprehensive and expertly annotated protein sequence database.  The primary sources of PSD data are sequences from GenBank/EMBL/DDBJ translations, published literature, and direct submission to PIR-International. ...
Nutritional Assessment & Clinical Application Part 2
Nutritional Assessment & Clinical Application Part 2

... • May be useful indicator of nutritional status in “healthy” person. • Not a good indicator of protein status during critical illness (due to acute phase response) • Long half life (14-20 days) and large body pool  slow to respond to improvements in clinical status ...
LOF1 and Interacting Transcription Factors in Plant Development
LOF1 and Interacting Transcription Factors in Plant Development

... Transcription factors (TFs) help ensure proper gene expression in developing tissues, and thus play a role in plant development and plant architecture. LATERAL ORGAN FUSION1, or LOF1, is a TF expressed in the organ boundaries of Arabidopsis thaliana. lof1 mutants have fused axillary branches and cau ...
Characterisation of Milk Proteins in Confectionery Products
Characterisation of Milk Proteins in Confectionery Products

... although representing only a small percentage (3-4 %) of its total composition are considered to possess unique structural and functional properties which will influence these final quality parameters as well as affecting processing. Of particular impor tance in confectionery are organoleptic, hydra ...
Slides
Slides

... EC 3 : hydrolases (enzymes that use water to break up some other molecules ) EC 3.4 : hydrolases that act on peptide bonds EC 3.4.11 : hydrolases that cleave off the aminoterminal amino acid from polypeptide EC 3.4.11.4 : hydrolases that cleave off the aminoReference: terminal end from a tripeptide ...
Protein kinase inhibition: natural and synthetic variations on a theme
Protein kinase inhibition: natural and synthetic variations on a theme

... kindly provided by Nikola Paveltich (Memorial Sloan-Kettering Cancer Center, New York). ...
File
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... If the pH is higher or lower, it will affect the chem rxns that take place in the cells. • buffers – weak acids or bases that are used to control the acidity or basicity of a substance; they prevent sharp sudden ...
Intro to Cell Biology - local.brookings.k12.sd.us
Intro to Cell Biology - local.brookings.k12.sd.us

... recognize that different structures perform different functions; describe the life cycle of somatic cells; identify DNA as the structure that carries the genetic code; ...
Functional Assay for Botulinum Neurotoxin Type A
Functional Assay for Botulinum Neurotoxin Type A

... and SNAPtide® (Mca/Dnp). These assays were run with one hour toxin binding at room temperature and a six hour room temperature cleavage reaction. Both substrates show a linear response to the toxin concentrations. Three hundred picograms of BTA can be detected with either peptide. ...
Theory and practice of size exclusion chromatography for
Theory and practice of size exclusion chromatography for

... volume to the flow rate needs to be decreased. Reducing the column size and increasing the flow rate are the straightforward ways for performing fast SEC analysis [34]. Shortening the column length has been applied in fast SEC analysis to keep the backpressure at a reasonable level even at high flow ra ...
Pierce Trypsin Protease, MS Grade
Pierce Trypsin Protease, MS Grade

... Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package inserts (“Documentation”). No claim of suitability for use in applicati ...
Mammalian Two-Hybrid Assay Kit
Mammalian Two-Hybrid Assay Kit

... should be optimized with a reporter plasmid before performing the assays. Sufficient quantity of plasmid is included for several optimization experiments. Because the luciferase assay is very sensitive, various transfection methods, such as calcium phosphate precipitation and lipid-mediated transfec ...
Equilibrium in CHNOSZ
Equilibrium in CHNOSZ

... where K is the equilibrium constant and Q is the activity quotient of species in the reaction (log in this text denotes base-10 logarithms, i.e. log10 in R). (1) Reference activity User-defined (usually equal) activities of species of interest. (1) Reference affinity (Aref ) Chemical affinity of for ...
Archives of microbiology
Archives of microbiology

... of metal clusters that are associated with nitrogenase (Howard and Rees 2006). Thus, the availability of nutritional iron might impose restrictions on the expression, assembly, and function of an effective nitrogenase complex. Iron depletion in the mutant exbD gene from Herbaspirillum seropedicae th ...
full text pdf
full text pdf

... the total serum protein. Its structure contains many hydrophobic pockets which bind a variety of biological molecules. Several examples follow: –– By binding to albumin, fatty acids can be transported at high concentrations (free fatty acids are soluble to about 10-6 M while the fatty acid - albumin ...
1055 BIOTECHNOLOGYDERIVED ARTICLES—PEPTIDE
1055 BIOTECHNOLOGYDERIVED ARTICLES—PEPTIDE

... Other Parameters— Temperature control of the column is usually necessary to achieve good reproducibility. The flow rates for the mobile phases range from 0.1 to 2.0 mL per minute, and the detection of peptides is performed with a UV detector at 200 to 230 nm. Other methods of detection have been use ...
Introns and mutations
Introns and mutations

... A conservative amino acid substitution is one that should not affect the function of the protein. However, this is often not easy to predict. In one context, leucine and isoleucine might be interchangeable, while in another, substituting isoleucine for leucine could lead to an inactive protein. That ...
Bacterial cultivation - Furry Helpers Pet Sitting
Bacterial cultivation - Furry Helpers Pet Sitting

... Cells will prefer to use sugars for energy when they are present, sparing the proteins; Proteins will be used only after the sugar source has been depleted. ...
MEMS Processing
MEMS Processing

... Basic MEMS materials Silicon and its derivatives, mostly • Micro-electronics heritage Si is a good semiconductor, properties can be tuned Si oxide is very robust Si nitride is a good electrical insulator ...
antibodies - immunology.unideb.hu
antibodies - immunology.unideb.hu

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Protein adsorption



Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.
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