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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 23. Mention the parameters to be considered for primer designing for PCR applications. How do you design primers using Primer3 software? 24. Differentiate RFLP and RAPD. How can the former technique be used for detection of genetic disorders and epidemiological typing? 25. Enumerate the steps involv ...
Biology Fact Sheet
Biology Fact Sheet

... Sex Chromosomes: The pair of chromosomes that determine the gender of an individual. XX-Female; XY- Male Codominance - Two different alleles at a locus are responsible for different phenotypes, and both alleles affect the phenotype of the heterozygote. Blood type AB-both the A and B alleles contribu ...
lab 10 SCA HO
lab 10 SCA HO

Genetically Modified Organisms
Genetically Modified Organisms

... What are GM’s? are a result of technology that has altered the DNA of living organisms (animals, plants or bacteria) Other terms that mean the same thing: ...
Expressed sequence tag (EST) - Washington State University
Expressed sequence tag (EST) - Washington State University

Chapter 10: Genes and Chromosomes
Chapter 10: Genes and Chromosomes

... seriously affect gene function • Any chemical change that affects the DNA molecule has the potential to produce gene mutations • The smallest changes, known as point mutations, affect no more than a single nucleotide • However, if a single base is inserted or deleted, the groupings are shifted for e ...
5b . Students know how to apply base-pairing rules to explain... semiconservative replication and transcription of information from DNA into mRNA.
5b . Students know how to apply base-pairing rules to explain... semiconservative replication and transcription of information from DNA into mRNA.

... editing  ribosomes read mRNA as it is being transcribed ...
Molecular basis of evolution.
Molecular basis of evolution.

... (and codons corresponding to these tRNA). Codon usage bias is the same for all highly expressed genes in the same organism. - Mutation pressure. Difference between mutation rates between GC  AT and AT  GC. GC-content is different in different organisms. ...
Fanconi Anemia
Fanconi Anemia

... The protein responsible for Type A FA is titled FANCA. When one of the three previously mentioned mutations occurs, the FANCA protein is transcribed. The transcription of this protein results in the phenotype described earlier. ...
03-131 F 2013 Final Exam Name:_________________________
03-131 F 2013 Final Exam Name:_________________________

... The signal sequence will cause the ribosome that is synthesizing the protein to attach to the membrane of the rough endoplasmic reticulum (ER) and the protein will be exported into the lumen of the rough ER. The protein will then travel to the golgi in a membrane vesicle, and then the vesicle will f ...
Test Your Knowledge – Chapter 3 Name
Test Your Knowledge – Chapter 3 Name

... 1. Cellulose is a ______ made of many______. a. polypeptide … monomers b. carbohydrate … fatty acids c. polymer … glucose molecules d. protein … amino acids e. lipid … triglycerides 2. In a hydrolysis reaction ______, and in this process water is ______. a. a polymer breaks up to form monomers … con ...
Chapter 10 - Saint Demetrios Astoria School
Chapter 10 - Saint Demetrios Astoria School

... • Orchestration of gene expression during development (cont’d.): – The nucleus turns on master genes based on its position relative to the gradient proteins – Master gene products also form gradients, further influencing which additional master genes the nucleus will turn on ...
3. polygenic traits
3. polygenic traits

... or allele encodes the particular trait. What we can observe is that an allele and a phenotype variant co-occur in a given cellular and genetic environment. We can raise the question of how it is possible that the 1 gene, 1 phenotype, or more precisely the 1 allele, 1 phenotype variant relationship c ...
Lecture 27
Lecture 27

... nucleus so that only properly processed mRNAs are exported to the cytoplasm •The role of introns is still controversial but the favored hypothesis is that they arose early in evolution and allowed recombination between mini-genes. They have been almost eliminated in bacteria and many lower eukaryote ...
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17GeneToProtein

... Ch. 17:From Gene to Protein ...
Scientific Writing
Scientific Writing

... The protein encoded by the oncogene is often part of a fusion protein with other virally-encoded amino acids attached ...
Semester 2 Exam Review
Semester 2 Exam Review

... (HINT: think about where they take place, what happens in each, do they use  DNA or RNA as a template, what is the end product….LOTS to put here) ...
Cellular Reproduction - Genomic DNA
Cellular Reproduction - Genomic DNA

... Each copy of a homologous pair of chromosomes originates from a dierent parent; therefore, the genes themselves are not identical. The variation of individuals within a species is due to the specic combination of the genes inherited from both parents. Even a slightly altered sequence of nucleotide ...
FINAL EXAM - 09 December 2005
FINAL EXAM - 09 December 2005

... A cell is composed of compounds that include proteins, nucleic acids, lipids, and carbohydrates. A cell is capable of reproduction, but when the compounds of the cell are isolated, none of them can reproduce. Therefore, cell reproduction is an example of ... A. B. C. D. E. ...
Wildlife Forensics Pre-Visit Lesson This pre
Wildlife Forensics Pre-Visit Lesson This pre

Voting: In Your Genes? - James Fowler
Voting: In Your Genes? - James Fowler

... environmental factors such as whether more of the identical than fraternal twins were living together, which might inflate their degree of similarity. The researchers concluded that the correlation for voting was much higher between pairs of identical (.71) than fraternal (.50) twins. From this they ...
DNA Replication - Toronto District Christian High School
DNA Replication - Toronto District Christian High School

... Professor Michael Smith Then, the desired mutations were carefully selected. This process was time-consuming. It would be much more efficient if a researcher could simply select a particular gene and mutate it as desired. Such a system was first proposed in the 1970s by Canadian scientist Michael Sm ...
DNA Extraction Lab
DNA Extraction Lab

... 4. Answer the question in the purpose and give some explanation. 5. Create an observation table. Introduction In this investigation, you will isolate DNA from strawberries and liver. DNA is 100 000 times longer than the cell itself, but only takes up about 10% of the space in the cell. It achieves t ...
Analysis of large gene expression datasets using bioinformatics The
Analysis of large gene expression datasets using bioinformatics The

... Analysis of large gene expression datasets using bioinformatics Russell Johnson week #11 ...
Margaret Dayhoff - Georgia Tech ISyE
Margaret Dayhoff - Georgia Tech ISyE

... The BLAST programs are widely used tools for searching protein and DNA databases for sequence similarities.  For protein comparisons, a variety of definitional, algorithmic and statistical refinements described here  permits the execution time of the BLAST programs to be decreased substantially whil ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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