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statgen3
statgen3

... material on which natural selection can act. ...
Margaret Dayhoff - Georgia Tech ISyE
Margaret Dayhoff - Georgia Tech ISyE

... The BLAST programs are widely used tools for searching protein and DNA databases for sequence similarities.  For protein comparisons, a variety of definitional, algorithmic and statistical refinements described here  permits the execution time of the BLAST programs to be decreased substantially whil ...
Lutz Heide, Pharmaceutical Institute, Tübingen University
Lutz Heide, Pharmaceutical Institute, Tübingen University

... Retrospective: It was discovered in the mid 1950s and for many years it was tought to exist only in eukaryotes. Period of controversy: Serine, threonine and tyrosine phosphorylation is the most common type of phosphorylation in eukaryotes, on contrary, in bacteria phosphorylation occurs predominantl ...
Notes
Notes

...  Some traits are controlled by __________________________________ acting together as a group to produce a single trait.  Examples: ___________________________________, _______________________________, _______________________________ 6. Sex Chromosomes  The __________________ pair of chromosomes a ...
molecular biology
molecular biology

... endonuclease will digest a nucleic acid molecule any where in the middle of the chain. As a result the oligonucleotides are formed. The smallest molecule that can be formed as the action of an endonuclease is a dinucleodtide. An exonuclease, on the other hand, digests the nucleic acid either from it ...
Document
Document

... Select a subset of your MSA Redo the tree Repeat this operation N times (100 or 1000 times if you can) Compute a consensus tree of the N trees Measure how many of the N trees agree with the consensus tree on ...
Section 20.1
Section 20.1

... • The polymerase chain reaction (PCR) copies a specific DNA sequence through in vitro reactions that can amplify target DNA sequences present in very small ...
Exam 2 Answer Key
Exam 2 Answer Key

... lifetime to their offspring. For many years, biology textbooks have depicted Lamarck as having been right about evolution being real, but wrong about the mechanism. How do recent discoveries in the field of epigenetics indicate that Lamarck may not have been so wrong after all? The field of epigenet ...
Dot plot - TeachLine
Dot plot - TeachLine

... Compare new genes to known ones Compare genes from different species information about evolution ...
2/14 - Utexas
2/14 - Utexas

... mitochondria and chloroplasts from freeliving bacteria to cellular organelles CB 26.13 ...
Introduction to Genetics
Introduction to Genetics

... Studies the way in which genetic/hereditary information is passed on from parents to offspring ...
Homework 3 - Haixu Tang`s Homepage
Homework 3 - Haixu Tang`s Homepage

... We used a shotgun strategy to sequence an unknown DNA molecule. Assume we obtained reads (fragments) with coverage 10, i.e. in average, each nucleotide in the target DNA is covered by 10 different reads (as in the following figure). Suppose the distribution of sequencing errors is dependent on the r ...
Cold Spring Harbor Laboratory Scientists Produce High
Cold Spring Harbor Laboratory Scientists Produce High

... transcriptome analysis in plants. The Iso-Seq protocol allows scientists to generate long reads covering full-length gene transcripts, providing a more accurate view of gene structure, gene expression, and important mechanisms such as alternative gene splicing. Iso-Seq analysis of SMRT Sequencing da ...
5. Genetics
5. Genetics

... alleles whose loci are closely linked may travel together through many generations without being separated. ...
MPS1 Antibody / Metallopan-stimulin 1 (R32561)
MPS1 Antibody / Metallopan-stimulin 1 (R32561)

... gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm ...
Megan Silas - University of Illinois at Chicago
Megan Silas - University of Illinois at Chicago

... Used to amplify short fragments of DNA without using cells Introduce primer to the plasmid containing the wild type cDNA Complementary regions will anneal Elongation will create a new plasmid containing the desired mutation that was initially present in the primer ...
Presentation
Presentation

... glycogenolysis.  These processes are regulated by the pancreas, intestine, liver, kidneys and muscle.  Hyperglycaemia can result from disruption of the hormones involved in glucose regulation such as insulin or glucagon or from dysfunction of the organs involved in glucose homeostasis. ...
Biological and Environmental Foundations
Biological and Environmental Foundations

...  Only one allele affects the child’s characteristics  Dominant allele – one that affects the child’s characteristics  Recessive – one that has no effect on the child’s ...
Easy Transformation of E.coli Using GPF
Easy Transformation of E.coli Using GPF

... resistance example just described. Once a plasmid enters the cell, the cellular machinery is used to replicate the plasmid, sometimes as many as 800 copies per cell. These are called high copy number plasmids. A plasmid is relatively small, typically only a few thousand base pairs in length. This is ...
Manipulation DNA
Manipulation DNA

... DNA by cutting it in specific places. DNA ligase is an enzyme that exist in all cells and is responsible for joining together strands of DNA. Scientists use restriction enzymes to cut DNA at a specific sequence called recognition sites. They can rejoin the cut strands with DNA ligase to make a new c ...
Revision exercise
Revision exercise

... the structure and function of DNA. James Watson ...
doc bio 202 2009
doc bio 202 2009

... molecule). This type of mutation is called a: a. a leaky mutation b. a dominant-negative mutation c. recessive mutation d. haplo-insufficient mutation e. haplo-sufficient mutation Answer b. Note: “Haplo-insufficient mutation” is an incorrect answer. While this is a dominant mutation, the phenotypic ...
Protein Synthesis Study Questions
Protein Synthesis Study Questions

... 7. Describe the 3 steps involved in making RNA. 8. What is the name of the process that makes RNA? 9. Describe the 3 steps involved in RNA processing. 10. What is the purpose of RNA processing? 11. Describe the 3 steps involved in using RNA to make proteins. 12. What is the name of the process that ...
The HNF-3 Gene Family of Transcription Factors in Mice: Gene
The HNF-3 Gene Family of Transcription Factors in Mice: Gene

Student Exploration Sheet: Growing Plants
Student Exploration Sheet: Growing Plants

... Just as a construction crew uses blueprints to build a house, a cell uses DNA as plans for building proteins. In addition to DNA, another nucleic acid, called RNA, is involved in making proteins. In the RNA and Protein Synthesis Gizmo™, you will use both DNA and RNA to construct a protein out of ami ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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