Label-free and redox proteomic analyses of the
... intracellular lipids in Rhodococcus strains were performed by TLC. For intracellular analysis, 4–5 mg of lyophilized cells were extracted with a mixture of chloroform and methanol (2 : 1, v/v) for 120 min at 4 uC. Fifteen to thirty microlitres of extracts (depending on culture conditions) were separ ...
... intracellular lipids in Rhodococcus strains were performed by TLC. For intracellular analysis, 4–5 mg of lyophilized cells were extracted with a mixture of chloroform and methanol (2 : 1, v/v) for 120 min at 4 uC. Fifteen to thirty microlitres of extracts (depending on culture conditions) were separ ...
Human Metabolism: Macronutrients Instructors: Rosalind Coleman
... Nutrition 600: Learning Objectives General Objectives: Students will be able to: 1. Explain how nutrients are transported in the blood and across cell membranes. 2. Explain how hormones transmit signals that regulate metabolic pathways. 3. Explain major metabolic pathways and critical regulatory poi ...
... Nutrition 600: Learning Objectives General Objectives: Students will be able to: 1. Explain how nutrients are transported in the blood and across cell membranes. 2. Explain how hormones transmit signals that regulate metabolic pathways. 3. Explain major metabolic pathways and critical regulatory poi ...
LESSON 11. СOMMUNICATION BETWEEN CELLS. MECHANISM
... LIPOPHILIC HORMONES are carried through the bloodstream by plasma proteins. They enter the cell by diffusion through the cell membrane. Inside the cell, they interact with intracellular receptors. As a result of this interaction, a structural change occurs in the receptor, and the hormone-receptor c ...
... LIPOPHILIC HORMONES are carried through the bloodstream by plasma proteins. They enter the cell by diffusion through the cell membrane. Inside the cell, they interact with intracellular receptors. As a result of this interaction, a structural change occurs in the receptor, and the hormone-receptor c ...
Chapter 7 How Cells Make ATP: Energy
... • Each two-carbon acetyl group combines with a four-carbon compound • Two CO2 molecules are removed • Energy captured as one ATP, three NADH, and one FADH2 per acetyl group (These will act as energy carriers for the electron transport chain) **So… 2 acetyl groups yields 4 CO2, 6 NADH, 2 FADH2, 2 ATP ...
... • Each two-carbon acetyl group combines with a four-carbon compound • Two CO2 molecules are removed • Energy captured as one ATP, three NADH, and one FADH2 per acetyl group (These will act as energy carriers for the electron transport chain) **So… 2 acetyl groups yields 4 CO2, 6 NADH, 2 FADH2, 2 ATP ...
Application of SVM to predict membrane protein types
... machine based on statistical learning theory. The most remarkable characteristics of SVMs are the absence of local minima, the sparseness of the solution, and the use of the kernel-induced feature spaces. The basic idea of applying SVMs to pattern classification can be outlined as follows. First, map ...
... machine based on statistical learning theory. The most remarkable characteristics of SVMs are the absence of local minima, the sparseness of the solution, and the use of the kernel-induced feature spaces. The basic idea of applying SVMs to pattern classification can be outlined as follows. First, map ...
Regulation of gene expression by polyunsaturated fatty acids
... lipids such as DAG, can increase cellular concentrations of second messengers such as cyclic AMP (cAMP), inositol triphosphate (IP3), and calcium (Ca) (7). These second messengers or their lipid precursors can all have effects on gene expression (8). Alternatively, free fatty acids and fatty acyl Co ...
... lipids such as DAG, can increase cellular concentrations of second messengers such as cyclic AMP (cAMP), inositol triphosphate (IP3), and calcium (Ca) (7). These second messengers or their lipid precursors can all have effects on gene expression (8). Alternatively, free fatty acids and fatty acyl Co ...
Analysis - The Journal of Cell Biology
... are modulated both by protein kinases and phosphatases. Although the importance of kinases in this process has long been recognized, an appreciation for the complex and fundamental role of phosphatases is more recent. Through extensive biochemical and genetic analysis, we now know that pathways are ...
... are modulated both by protein kinases and phosphatases. Although the importance of kinases in this process has long been recognized, an appreciation for the complex and fundamental role of phosphatases is more recent. Through extensive biochemical and genetic analysis, we now know that pathways are ...
syllabusbioch205 - OSU Biochemistry and Molecular Biology
... will be given Februry 26 and April 4, respectively. Each exam will be worth 100 points of the inresidence grade. These exams will be multiple choice and hosted by thetestingcenter. 2. Discussion Twelve contributions worth 5 points each required by Monday morning of each class week. These are to be c ...
... will be given Februry 26 and April 4, respectively. Each exam will be worth 100 points of the inresidence grade. These exams will be multiple choice and hosted by thetestingcenter. 2. Discussion Twelve contributions worth 5 points each required by Monday morning of each class week. These are to be c ...
Sphingolipid homeostasis in the web of metabolic routes
... survival as demonstrated by the existence of sphingolipidoses, human diseases generated by the defects in these pathways [3]. 2.3. Regulation of sphingolipid synthesis Although SLs are essential players in cell homeostasis the regulatory mechanisms controlling this pathway have only recently begun t ...
... survival as demonstrated by the existence of sphingolipidoses, human diseases generated by the defects in these pathways [3]. 2.3. Regulation of sphingolipid synthesis Although SLs are essential players in cell homeostasis the regulatory mechanisms controlling this pathway have only recently begun t ...
Signalling drought in guard cells
... induces production of H2O2. If H2O2 production is blocked by an inhibitor, ABA induction of the Ca2+ channel or stomatal closure is also blocked, implicating H2O2 production in the ABA signalling pathway between ABA and Ca2+ elevation. It is not known if the Ca2+ channels identified by these two stu ...
... induces production of H2O2. If H2O2 production is blocked by an inhibitor, ABA induction of the Ca2+ channel or stomatal closure is also blocked, implicating H2O2 production in the ABA signalling pathway between ABA and Ca2+ elevation. It is not known if the Ca2+ channels identified by these two stu ...
PROTEIN FOLDING
... Levinthal’s proposal: There SHOULD be a defined folding pathway through a defined series of intermediates, instead of a fully random process ...
... Levinthal’s proposal: There SHOULD be a defined folding pathway through a defined series of intermediates, instead of a fully random process ...
Re-routing the huntingtin protein inside cells
... Huntingtin - and its evil twin Though Huntington’s disease is due to a genetic abnormality, we know that the damage it causes to cells is due to the mutant huntingtin protein. The HD gene is a set of instructions for making the huntingtin protein. In people with HD, and those who are destined to dev ...
... Huntingtin - and its evil twin Though Huntington’s disease is due to a genetic abnormality, we know that the damage it causes to cells is due to the mutant huntingtin protein. The HD gene is a set of instructions for making the huntingtin protein. In people with HD, and those who are destined to dev ...
Engineering of metabolic pathways by artificial enzyme channels
... catalyzed by free-floating, isolated enzymes but by multienzyme complexes. In these microcompartments, several enzymes form so-called metabolons or metabolic channels overcoming flux imbalances, diffusion and loss of intermediates, and release of toxic intermediates (Figure 2; Conrado et al., 2008 ...
... catalyzed by free-floating, isolated enzymes but by multienzyme complexes. In these microcompartments, several enzymes form so-called metabolons or metabolic channels overcoming flux imbalances, diffusion and loss of intermediates, and release of toxic intermediates (Figure 2; Conrado et al., 2008 ...
Gene Section S100A2 (S100 calcium binding protein A2)
... exon 2 codes for the N-terminal 47 amino acids and exon 3 codes for the C-terminal 50 amino acids. The S100A2 gene has a total length of 4723 bp and seven splice variants, which can be categorized into five variants with protein product and two transcripts without an open-reading frame. Four of the ...
... exon 2 codes for the N-terminal 47 amino acids and exon 3 codes for the C-terminal 50 amino acids. The S100A2 gene has a total length of 4723 bp and seven splice variants, which can be categorized into five variants with protein product and two transcripts without an open-reading frame. Four of the ...
CELLULAR RESPIRATION
... Cellular Respiration - Cells transfer energy from food molecules to ATP, the byproduct is water and carbon dioxide ...
... Cellular Respiration - Cells transfer energy from food molecules to ATP, the byproduct is water and carbon dioxide ...
Luiziana Ferreira da Silva Lab of Bioproducts Department of Microbiology
... Biotechnological applications Purified granules can be chemically converted to a derivative with reduced arginine content or to completely biodegradable poly(aspartic acid) , which can be used as a substitute for nonbiodegradable polyacrylates with many technical and medical applications ...
... Biotechnological applications Purified granules can be chemically converted to a derivative with reduced arginine content or to completely biodegradable poly(aspartic acid) , which can be used as a substitute for nonbiodegradable polyacrylates with many technical and medical applications ...
Journal: Applied Microbiology and Biotechnology
... nucleotides for biosynthesis. We finally conclude that with sufficient availability of substrates the dynamics of glucose and glutamine/glutamate metabolism is influenced mainly by the cellular growth regime and regulatory function of key enzymes. ...
... nucleotides for biosynthesis. We finally conclude that with sufficient availability of substrates the dynamics of glucose and glutamine/glutamate metabolism is influenced mainly by the cellular growth regime and regulatory function of key enzymes. ...
Functional and quantitative proteomics using SILAC
... labelling is very high — quantitation is straightforward. SILAC does require adapting one’s cell line to dialyzed serum, and further steps might have to be taken if cells don’t adapt well. However, this step can be checked without labelled amino acids. The SILAC approach is not necessarily expensive ...
... labelling is very high — quantitation is straightforward. SILAC does require adapting one’s cell line to dialyzed serum, and further steps might have to be taken if cells don’t adapt well. However, this step can be checked without labelled amino acids. The SILAC approach is not necessarily expensive ...
Metabolic Minimap article
... 120° stages. At each stage each of the -subunits in turn change conformation; L changes to T (after binding ADP and phosphate), T to O, and O to L (after releasing ATP). The new L site then binds new ADP and phosphate and begins a new reaction sequence. One complete revolution of F0 therefore resul ...
... 120° stages. At each stage each of the -subunits in turn change conformation; L changes to T (after binding ADP and phosphate), T to O, and O to L (after releasing ATP). The new L site then binds new ADP and phosphate and begins a new reaction sequence. One complete revolution of F0 therefore resul ...
4.3. monosaccharides
... strong interactions between water molecules and the polyanionic complex. It is present in cartilage and tendon, vitreous humour (eyes), extracellular matrix, mucosal surface and synovial fluid. Chondroitin sulfate. It promotes tension resistance in cartilage, tendon and arteries. It is present in ...
... strong interactions between water molecules and the polyanionic complex. It is present in cartilage and tendon, vitreous humour (eyes), extracellular matrix, mucosal surface and synovial fluid. Chondroitin sulfate. It promotes tension resistance in cartilage, tendon and arteries. It is present in ...
UNIT 4. CARBOHYDRATES
... strong interactions between water molecules and the polyanionic complex. It is present in cartilage and tendon, vitreous humour (eyes), extracellular matrix, mucosal surface and synovial fluid. Chondroitin sulfate. It promotes tension resistance in cartilage, tendon and arteries. It is present in ...
... strong interactions between water molecules and the polyanionic complex. It is present in cartilage and tendon, vitreous humour (eyes), extracellular matrix, mucosal surface and synovial fluid. Chondroitin sulfate. It promotes tension resistance in cartilage, tendon and arteries. It is present in ...