Distinct C-terminal Amino Acid Sequence Motifs Serve
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
Recent Developments in Patent Law
... – General method for isolating a gene that encodes a particular protein where at least a short amino acid sequence of the encoded protein is known; and – General method employing a probe specifically corresponding to the known amino acid sequence for the protein. – Discloses that it is advantageous ...
... – General method for isolating a gene that encodes a particular protein where at least a short amino acid sequence of the encoded protein is known; and – General method employing a probe specifically corresponding to the known amino acid sequence for the protein. – Discloses that it is advantageous ...
Comparison of the activities of protein disulphide
... sequence (one residue only), suggesting there are other more relevant intrinsic differences between them. A further hint that the active site can tolerate a range of residues in the position preceding the second cysteine residue (see Fig. 2) is the finding by Gleason et al. (1990) that a mutant thio ...
... sequence (one residue only), suggesting there are other more relevant intrinsic differences between them. A further hint that the active site can tolerate a range of residues in the position preceding the second cysteine residue (see Fig. 2) is the finding by Gleason et al. (1990) that a mutant thio ...
Lecture 1 - "Hudel" Luecke
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
Mitochondrial trans-2-Enoyl-CoA Reductase of Wax Ester
... ml of buffer D, proteins were eluted in a 20-ml gradient of 0 –1 M KCl in buffer D, fractions of 280 l were collected. Active fractions were concentrated by ultrafiltration (Amicon Ultra, Millipore), applied in six runs to a continuous, preparative 6-cm 6% native polyacrylamide gel (Mini-Prepcell, ...
... ml of buffer D, proteins were eluted in a 20-ml gradient of 0 –1 M KCl in buffer D, fractions of 280 l were collected. Active fractions were concentrated by ultrafiltration (Amicon Ultra, Millipore), applied in six runs to a continuous, preparative 6-cm 6% native polyacrylamide gel (Mini-Prepcell, ...
PPTX - Bonham Chemistry
... To perform a step mash with an acid rest: – Add hot water to your grain bill to achieve an appropriate rest temperature for an acid rest. Hold the mash at this temperature until the proper pH is achieved . Note this may take more than one hour. – Add hot water infusion or direct heat to raise the ma ...
... To perform a step mash with an acid rest: – Add hot water to your grain bill to achieve an appropriate rest temperature for an acid rest. Hold the mash at this temperature until the proper pH is achieved . Note this may take more than one hour. – Add hot water infusion or direct heat to raise the ma ...
Safety assessment - Federal Register of Legislation
... The assessment of this Application identified no public health and safety concerns. On the basis of the available evidence, including detailed studies provided by the Applicant, food derived from corn line 88017 is considered as safe and wholesome as food derived from other commercial corn varieties ...
... The assessment of this Application identified no public health and safety concerns. On the basis of the available evidence, including detailed studies provided by the Applicant, food derived from corn line 88017 is considered as safe and wholesome as food derived from other commercial corn varieties ...
A motif and amino acid bias bioinformatics
... Lamport, 1994; Lamport et al., 2011). The extent of glycosylation of PRPs remains unclear as it has ...
... Lamport, 1994; Lamport et al., 2011). The extent of glycosylation of PRPs remains unclear as it has ...
Expanding the Genetic Code
... chain. Typical modifications include the oxidation or alkylation of cysteine residues, acylation of either the N-terminal aamino group or the e-amino group of lysine, and the condensation of amines with the carboxylate groups of aspartate or glutamate. In some cases it is possible to exploit the uni ...
... chain. Typical modifications include the oxidation or alkylation of cysteine residues, acylation of either the N-terminal aamino group or the e-amino group of lysine, and the condensation of amines with the carboxylate groups of aspartate or glutamate. In some cases it is possible to exploit the uni ...
Engineering subunit association of multisubunit proteins
... could be reduced significantly by deleting a loop from G113 through W120 that should have no apparent contact with biotin in a dimeric molecule. The resulting protein, containing both the H127D mutation and the loop deletion, formed a soluble dimeric streptavidin in the presence of biotin. There are ...
... could be reduced significantly by deleting a loop from G113 through W120 that should have no apparent contact with biotin in a dimeric molecule. The resulting protein, containing both the H127D mutation and the loop deletion, formed a soluble dimeric streptavidin in the presence of biotin. There are ...
Substrate Specificity of Human Kallikreins 1 and 6
... anti-pIII antisera. The TAFRSAYG sequence was efficiently cleaved under these conditions, resulting in loss of the Flag tag. In contrast, phage encoding SSYISNYG remained intact (Fig. 1). Thus, KLK6 does not cleave pIII nor does it affect the integrity of the phage, but it can recognize substrate se ...
... anti-pIII antisera. The TAFRSAYG sequence was efficiently cleaved under these conditions, resulting in loss of the Flag tag. In contrast, phage encoding SSYISNYG remained intact (Fig. 1). Thus, KLK6 does not cleave pIII nor does it affect the integrity of the phage, but it can recognize substrate se ...
University of Groningen Cross-linking of dimeric CitS and GltS
... described before (Dobrowolski et al. 2007, Dobrowolski and Lolkema 2009). Plasmid pGltS-BAD1 was obtained by ligation of the BAD encoding fragment with NcoI overhangs into the NcoI site of pN356C situated in between the His-tag and GltS encoding sequences. Plasmid pGltS-BAD206 was obtained in a simi ...
... described before (Dobrowolski et al. 2007, Dobrowolski and Lolkema 2009). Plasmid pGltS-BAD1 was obtained by ligation of the BAD encoding fragment with NcoI overhangs into the NcoI site of pN356C situated in between the His-tag and GltS encoding sequences. Plasmid pGltS-BAD206 was obtained in a simi ...
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of
... ABSTRACT: Maltose permease is required for maltose transport into Saccharomyces cells. Glucose addition to maltose-fermenting cells causes selective delivery of this integral plasma membrane protein to the yeast vacuole via endocytosis for degradation by resident proteases. This glucose-induced degr ...
... ABSTRACT: Maltose permease is required for maltose transport into Saccharomyces cells. Glucose addition to maltose-fermenting cells causes selective delivery of this integral plasma membrane protein to the yeast vacuole via endocytosis for degradation by resident proteases. This glucose-induced degr ...
Introduction to the BLAST Suite and BLASTN
... The DEFINITION field is usually a brief description of the sequence, its origin, and any additional information that may prove valuable to the reader. The definition line for this record is unusually long, clearly taken from the title of the patent: “A group of genes which is differentially expresse ...
... The DEFINITION field is usually a brief description of the sequence, its origin, and any additional information that may prove valuable to the reader. The definition line for this record is unusually long, clearly taken from the title of the patent: “A group of genes which is differentially expresse ...
Raw Glycerol as Substrate for the Production of Yeast Biomass
... 95% confidence using raw glycerol; however, C. lipolytica NRRL Y-1095 showed a lower specific growth rate, differing statistically from other strains, at 95% confidence, using pure glycerol. In general, the use of raw glycerol and, consequently, the presence of impurities such as ethanol, soaps and ...
... 95% confidence using raw glycerol; however, C. lipolytica NRRL Y-1095 showed a lower specific growth rate, differing statistically from other strains, at 95% confidence, using pure glycerol. In general, the use of raw glycerol and, consequently, the presence of impurities such as ethanol, soaps and ...
Biological significance of structural differences between two highly
... The structure of Uev1D30 was solved by molecular replacement using a poly-alanine model of Mms2 (PDB accession number: 1J74). The density for the core 140 residues of Uev1D30 was well ordered (Fig. 1B) and adopted a structure very similar to Mms2 [8] with an RMSD of 0.5 Å. This was expected given th ...
... The structure of Uev1D30 was solved by molecular replacement using a poly-alanine model of Mms2 (PDB accession number: 1J74). The density for the core 140 residues of Uev1D30 was well ordered (Fig. 1B) and adopted a structure very similar to Mms2 [8] with an RMSD of 0.5 Å. This was expected given th ...
Slide 1
... transmission and nerve activity. Therefore, PCs are critical for the health and well being of organisms. •Seven subfamilies of PCs have been identified: PC1/PC3, PC2, PC4, PACE4, PC5, Furin, and PC7. The subfamilies may be active in different conditions (e.g. pH level) and organs (e.g. PC7 can be fo ...
... transmission and nerve activity. Therefore, PCs are critical for the health and well being of organisms. •Seven subfamilies of PCs have been identified: PC1/PC3, PC2, PC4, PACE4, PC5, Furin, and PC7. The subfamilies may be active in different conditions (e.g. pH level) and organs (e.g. PC7 can be fo ...
Appendix 1: PBS listings of Nutritional Products
... The continuing treatment item codes allow for use in same conditions as the initial treatment item codes. Patients receiving continuing treatment of cows’ milk enteropathy and who are less than 24 months old (including with failure to thrive) or IgE-mediated cows’ milk protein allergy must be treate ...
... The continuing treatment item codes allow for use in same conditions as the initial treatment item codes. Patients receiving continuing treatment of cows’ milk enteropathy and who are less than 24 months old (including with failure to thrive) or IgE-mediated cows’ milk protein allergy must be treate ...
The phosphopantetheinyl transferases
... published. The work comprehensively described the identication of many PPTases using bioinformatics, the characterization of family II proteins by heterologous expression, purication and biochemical characterization and the clarication of the link between Sfp, EntD and Gsp in natural-product bios ...
... published. The work comprehensively described the identication of many PPTases using bioinformatics, the characterization of family II proteins by heterologous expression, purication and biochemical characterization and the clarication of the link between Sfp, EntD and Gsp in natural-product bios ...
Vanadium-Binding Proteins (Vanabins)
... organism because molecular biology has revealed that ascidians have key chordate features in common with the vertebrates. Moreover, their genome size is relatively small (~160 million bases) and they possess fundamentally all of basic developmental genes. They are, therefore, one of model organisms ...
... organism because molecular biology has revealed that ascidians have key chordate features in common with the vertebrates. Moreover, their genome size is relatively small (~160 million bases) and they possess fundamentally all of basic developmental genes. They are, therefore, one of model organisms ...
Structure-Based Prediction of Asparagine and Aspartate
... which is essential for the development of a drug candidate into a marketed product. Therapeutic proteins may degrade via asparagine (Asn) deamidation and aspartate (Asp) isomerization, but the factors responsible for such degradation remain poorly understood. We studied the structural properties of ...
... which is essential for the development of a drug candidate into a marketed product. Therapeutic proteins may degrade via asparagine (Asn) deamidation and aspartate (Asp) isomerization, but the factors responsible for such degradation remain poorly understood. We studied the structural properties of ...
Capacitation-associated Changes in Protein
... sperms were incubated in TALP devoid of BSA, and the Indirect immunofluorescence Immunofluorescence was employed to examine the percentage of sperms undergoing capacitation was subcellular localization of proteins phosphorylated in measured. When guinea pig sperms were incubated in TALP, tyrosine re ...
... sperms were incubated in TALP devoid of BSA, and the Indirect immunofluorescence Immunofluorescence was employed to examine the percentage of sperms undergoing capacitation was subcellular localization of proteins phosphorylated in measured. When guinea pig sperms were incubated in TALP, tyrosine re ...
PeptidePicker: a Tool for Determining Most Appropriate Peptides for
... scoring algorithm that reflects our knowledge in choosing best candidate peptides for an MRM experiment based on the uniqueness of the peptide in the targeted proteome, the chemophysio properties of each peptide, and whether it was previously observed or not. ...
... scoring algorithm that reflects our knowledge in choosing best candidate peptides for an MRM experiment based on the uniqueness of the peptide in the targeted proteome, the chemophysio properties of each peptide, and whether it was previously observed or not. ...
Scientific Opinion on Dietary Reference Values for protein
... both to high quality protein and to protein in mixed diets. For infants from six months, children and adolescents a factorial approach as proposed by WHO/FAO/UNU (2007) was accepted. For this, protein requirements for growth were estimated from average daily rates of protein deposition, assuming an ...
... both to high quality protein and to protein in mixed diets. For infants from six months, children and adolescents a factorial approach as proposed by WHO/FAO/UNU (2007) was accepted. For this, protein requirements for growth were estimated from average daily rates of protein deposition, assuming an ...