Farnesyl diphosphate synthase, the target for nitrogen
... in mammalian cells, and it has been concluded that the enzymes are cytosolic [10]. Hence, at present, the intracellular location of the mammalian mevalonate pathway enzymes remains unclear. The pathway has also been investigated in plants and it appears that some, but not all, of the enzymes are per ...
... in mammalian cells, and it has been concluded that the enzymes are cytosolic [10]. Hence, at present, the intracellular location of the mammalian mevalonate pathway enzymes remains unclear. The pathway has also been investigated in plants and it appears that some, but not all, of the enzymes are per ...
Nucleic Acids Research
... deletion or site-directed mutagenesis analysis of these sequences. the 27 base changes that occur in this 240 nucleotide segment, most (17 of 27) are transitions which preserve a purine or pyrimidine, respectively, at the site of the change. Of the transversions that have occurred, most of these hav ...
... deletion or site-directed mutagenesis analysis of these sequences. the 27 base changes that occur in this 240 nucleotide segment, most (17 of 27) are transitions which preserve a purine or pyrimidine, respectively, at the site of the change. Of the transversions that have occurred, most of these hav ...
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... with mCherry was found to accumulate in high levels in the apoplastic space of the epidermis of cotyledons of wild-type seedlings, with only a weak signal detected in the vacuolar lumen [8]. However, in the mutant of KEEP ON GOING (KEG), a RING E3 ligase involved in abscisic acid signaling during gr ...
... with mCherry was found to accumulate in high levels in the apoplastic space of the epidermis of cotyledons of wild-type seedlings, with only a weak signal detected in the vacuolar lumen [8]. However, in the mutant of KEEP ON GOING (KEG), a RING E3 ligase involved in abscisic acid signaling during gr ...
PCNA Protein Expression during Spermatogenesis of the
... these forms is a 32-kDa protein with a pI value of 4.6 and the other is a 36-kDa protein with a pI of 4.7. To our knowledge, there has been no report of two forms to data of PCNA in any species. RT-PCR analysis using testis poly (A)+ RNA and primers corresponding to the 5’ and the 3’ end of the open ...
... these forms is a 32-kDa protein with a pI value of 4.6 and the other is a 36-kDa protein with a pI of 4.7. To our knowledge, there has been no report of two forms to data of PCNA in any species. RT-PCR analysis using testis poly (A)+ RNA and primers corresponding to the 5’ and the 3’ end of the open ...
Regulation of Translation by Essential Amino Acids and Glucose in
... they will be brilliant for each one of you and I hope they will cross often. I would like to acknowledge Dr. Julie Kim – Julie, learning from your expertise in the lab is the reason I know how to conduct good lab work today, a valued skill that I owe to you. A special thank you to the staff at the B ...
... they will be brilliant for each one of you and I hope they will cross often. I would like to acknowledge Dr. Julie Kim – Julie, learning from your expertise in the lab is the reason I know how to conduct good lab work today, a valued skill that I owe to you. A special thank you to the staff at the B ...
Characterization of the chimeric seven
... wild-type BPR (491 nm) at pH 7. The absorption maxima of all the CFR isolates were between 515 and 522 nm at pH 7 (Table 2), which falls into the green light region. CFR 1 absorbs light maximally at 545 and 513, at pH 4 and 10, respectively (Fig. 4). The most blue-shifted CFR variant, CFR3, displaye ...
... wild-type BPR (491 nm) at pH 7. The absorption maxima of all the CFR isolates were between 515 and 522 nm at pH 7 (Table 2), which falls into the green light region. CFR 1 absorbs light maximally at 545 and 513, at pH 4 and 10, respectively (Fig. 4). The most blue-shifted CFR variant, CFR3, displaye ...
NMR studies of the methionine methyl groups in calmodulin
... [3]. NMR and X-ray studies of complexes of CaM with various CaM-binding domain peptides show that all the Met residues, with the possible exception of Met-76, are in contact with the hydrophobic face(s) of the bound peptides [8-12]. Oxidation studies of CaM's Met residues have revealed that all of t ...
... [3]. NMR and X-ray studies of complexes of CaM with various CaM-binding domain peptides show that all the Met residues, with the possible exception of Met-76, are in contact with the hydrophobic face(s) of the bound peptides [8-12]. Oxidation studies of CaM's Met residues have revealed that all of t ...
Deciphering the molecular basis of the specificity of protein
... carbohydrate antigens. Therefore, the need to comprehend these interactions in detail. Carbohydrate-binding proteins show a remarkable specificity, being able to discern between very similar glycan residues. In order to better understand this precise specificity, the spatial vicinity of common monos ...
... carbohydrate antigens. Therefore, the need to comprehend these interactions in detail. Carbohydrate-binding proteins show a remarkable specificity, being able to discern between very similar glycan residues. In order to better understand this precise specificity, the spatial vicinity of common monos ...
Modulation of the Oligomerization State of the Bovine F1
... Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using the oligonucleotide primers GAA GAA ACA CAA GGA AAA TGA GAT and TAG GAA TTC CAT ATG CGA TAC TTC CGA GCT CGT GCT AAA, respectively. The plas ...
... Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using the oligonucleotide primers GAA GAA ACA CAA GGA AAA TGA GAT and TAG GAA TTC CAT ATG CGA TAC TTC CGA GCT CGT GCT AAA, respectively. The plas ...
Nucleotide sequence and structural organization of
... homologous (one mismatch) to the D S O of RC plasmids pE194, pSH71, pLSl and pWVO1, was identified 440 bp upstream of the RepB start codon and is believed to be the DSO of pCI411. The observation that the plasmid construct pCI430 (Table 1 and Fig. l ) , which lacks this region, could not replicate i ...
... homologous (one mismatch) to the D S O of RC plasmids pE194, pSH71, pLSl and pWVO1, was identified 440 bp upstream of the RepB start codon and is believed to be the DSO of pCI411. The observation that the plasmid construct pCI430 (Table 1 and Fig. l ) , which lacks this region, could not replicate i ...
High pKa variability of cysteine residues in structural databases and
... these oxidations are effectively counteracted through natural defensive mechanisms [9]; however, intense and/or persistent levels of ROS stress ultimately lead to protein damage (misfolding, cross-linkages, inactivation of functional residues) [10,11]. A critically important aspect of Cys reactivity ...
... these oxidations are effectively counteracted through natural defensive mechanisms [9]; however, intense and/or persistent levels of ROS stress ultimately lead to protein damage (misfolding, cross-linkages, inactivation of functional residues) [10,11]. A critically important aspect of Cys reactivity ...
Formation of Monoterpenes in Antirrhinum majus
... Some plant species contain multiple GGPPS and GGPPS-related enzymes (Okada et al., 2000; Burke and Croteau, 2002b). However, the A. majus floral EST database did not contain sequences with close similarity to M. piperita GPPS.LSU or to any other GGPPS proteins. To isolate cDNAs encoding a potential ...
... Some plant species contain multiple GGPPS and GGPPS-related enzymes (Okada et al., 2000; Burke and Croteau, 2002b). However, the A. majus floral EST database did not contain sequences with close similarity to M. piperita GPPS.LSU or to any other GGPPS proteins. To isolate cDNAs encoding a potential ...
Regulation of Cytochrome bd Expression in Mycobacterium
... and contains mycolic acid. Other Actinobacteria such as Corynebacterium, Rhodococcus and Nocardia have related properties. There are over 100 species that belong to the genus Mycobacterium and 16S rRNA sequence analyses reveals that all mycobacterial species are closely related (143, 159). Mycobacte ...
... and contains mycolic acid. Other Actinobacteria such as Corynebacterium, Rhodococcus and Nocardia have related properties. There are over 100 species that belong to the genus Mycobacterium and 16S rRNA sequence analyses reveals that all mycobacterial species are closely related (143, 159). Mycobacte ...
4. characterisation of novel proteins
... cassette encoding the two desaturase enzymes at a single genetic locus. The promoters and leader sequences used in the gene constructs are soybean-derived elements, known to be spatially and temporally active only in the developing seed. Data generated over three generations confirmed stability of t ...
... cassette encoding the two desaturase enzymes at a single genetic locus. The promoters and leader sequences used in the gene constructs are soybean-derived elements, known to be spatially and temporally active only in the developing seed. Data generated over three generations confirmed stability of t ...
SoyMilk - Soyfoods Association of North America
... Use 1-1/2 cups regular soymilk in place of 2 cups cow’s milk when making instant pudding (4-serving size), but the dessert may still be a little thinner than standard pudding. Pour your choice of regular, reduced-fat or non-fat soymilk over cereal, and add sliced fruit. Enjoy a glass of soy eggnog-s ...
... Use 1-1/2 cups regular soymilk in place of 2 cups cow’s milk when making instant pudding (4-serving size), but the dessert may still be a little thinner than standard pudding. Pour your choice of regular, reduced-fat or non-fat soymilk over cereal, and add sliced fruit. Enjoy a glass of soy eggnog-s ...
Angiotensin I-converting enzyme-inhibitory peptide fractions
... Table 1 shows the proximate analysis of the A. hypochondriacus grain and defatted flour used in this study. The present results show that crude protein (N 5.85) was not appreciably concentrated when the flour was defatted with acetone. On the contrary, ash and carbohydrates increased because of this ...
... Table 1 shows the proximate analysis of the A. hypochondriacus grain and defatted flour used in this study. The present results show that crude protein (N 5.85) was not appreciably concentrated when the flour was defatted with acetone. On the contrary, ash and carbohydrates increased because of this ...
Introduction to NMR spectroscopy
... size expressed in terms of tumbling (correlation) time -> there is an experimental limit to the size of the proteins one can determine by NMR ! we need tricks, more tricks.... ...
... size expressed in terms of tumbling (correlation) time -> there is an experimental limit to the size of the proteins one can determine by NMR ! we need tricks, more tricks.... ...
SBT202 - SibTech, Inc.
... Stability: EGF/Si is shipped lyophilized. After reconstitution, it should be aliquoted and frozen for long-term storage. Freezing-thawing should be avoided. Handling: Good laboratory technique should be employed in the safe handling of this product. This requires observing the following practices: 1 ...
... Stability: EGF/Si is shipped lyophilized. After reconstitution, it should be aliquoted and frozen for long-term storage. Freezing-thawing should be avoided. Handling: Good laboratory technique should be employed in the safe handling of this product. This requires observing the following practices: 1 ...
Phylogenomic Investigation of Phospholipid Synthesis in Archaea
... 15 carbons), geranylgeranyl diphosphate (GGPP, 20 carbons), and so forth. Different IPPSs are characterized by the allylic substrate that they accept (DMAPP, GPP, FPP, . . .) and by the stereochemistry of the double bonds, but the main characteristic used to classify them is the size of the products ...
... 15 carbons), geranylgeranyl diphosphate (GGPP, 20 carbons), and so forth. Different IPPSs are characterized by the allylic substrate that they accept (DMAPP, GPP, FPP, . . .) and by the stereochemistry of the double bonds, but the main characteristic used to classify them is the size of the products ...
Molecular genetics of the extracellular lipase of
... Introduction Lipases are triacylglycerol acylhydrolases (EC 3.1.1.3) that preferentially hydrolyse emulsified triglycerides. In organic solvents, lipases are also able to catalyse reverse reactions, e.g. the synthesis of esters, and transesterifications. The reaction mechanism postulated for the cat ...
... Introduction Lipases are triacylglycerol acylhydrolases (EC 3.1.1.3) that preferentially hydrolyse emulsified triglycerides. In organic solvents, lipases are also able to catalyse reverse reactions, e.g. the synthesis of esters, and transesterifications. The reaction mechanism postulated for the cat ...
Protein metabolism in exercising human with special reference to
... protein synthesis is generally depressed during exercise. After exercise, protein synthesis increases for periods up to 48 hours before declining to baseline values. Human muscle can oxidize at least seven amino acids. Of these amino acids, however, oxidation of only the branched chain amino acids ( ...
... protein synthesis is generally depressed during exercise. After exercise, protein synthesis increases for periods up to 48 hours before declining to baseline values. Human muscle can oxidize at least seven amino acids. Of these amino acids, however, oxidation of only the branched chain amino acids ( ...
Src protein–tyrosine kinase structure and regulation
... threonine kinases and protein–tyrosine kinases. Manning et al. [4] identified 478 typical and 40 atypical protein kinase genes in humans (total 518) that correspond to about 1.7% of all human genes. The family includes 385 protein–serine/threonine kinases, 90 protein–tyrosine kinases, and 43 tyrosine ...
... threonine kinases and protein–tyrosine kinases. Manning et al. [4] identified 478 typical and 40 atypical protein kinase genes in humans (total 518) that correspond to about 1.7% of all human genes. The family includes 385 protein–serine/threonine kinases, 90 protein–tyrosine kinases, and 43 tyrosine ...
Pepsinogen and Pepsin - The Journal of General Physiology
... for catalytic action on small substrate structures, such as dipeptides. This spatial proximity of the two or more critical side groups of the enzyme would be expected to be disturbed by denaturation of the protein since this is almost invariably accompanied by shape changes. A second approach to an ...
... for catalytic action on small substrate structures, such as dipeptides. This spatial proximity of the two or more critical side groups of the enzyme would be expected to be disturbed by denaturation of the protein since this is almost invariably accompanied by shape changes. A second approach to an ...
Identification of Bioactive Peptide Sequences from Amaranth
... but that expression data (such as existence of complementary deoxyribonucleic acids, real-time polymerase chain reaction, or Northern blots) indicate the existence of a transcript; (3) evidence inferred from homology, which indicates that the existence of a protein is probable because clear ortholog ...
... but that expression data (such as existence of complementary deoxyribonucleic acids, real-time polymerase chain reaction, or Northern blots) indicate the existence of a transcript; (3) evidence inferred from homology, which indicates that the existence of a protein is probable because clear ortholog ...