The Major Component of the Paraflagellar Rod of Trypanosoma
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
Characterization of the cDNA and Gene Coding for the Biotin
... has severa1 unusual features, including imperfect palindromes and polypyrimidine sequences, which may function in the transcriptional regulation of the 9\02 gene. ...
... has severa1 unusual features, including imperfect palindromes and polypyrimidine sequences, which may function in the transcriptional regulation of the 9\02 gene. ...
Development of prokaryotic cell-free systems for synthetic
... Separately, work from the Kim (HJ) lab in 2005 identified strains with the overexpression of molecular chaperones capable of reducing aggregation and improve solubility of eukaryotic proteins such as human erythropoietin (Kang et al., 2005). The work inserted plasmids to overexpress chaperone and he ...
... Separately, work from the Kim (HJ) lab in 2005 identified strains with the overexpression of molecular chaperones capable of reducing aggregation and improve solubility of eukaryotic proteins such as human erythropoietin (Kang et al., 2005). The work inserted plasmids to overexpress chaperone and he ...
Purification and Biochemical Characterization of Digestive Lipase in
... To date, only a few studies are available about digestive lipases in crustaceans, their detailed function in lipid metabolism, and methods of purification and characterization. Lipases from crab Carcinus mediterraneus (Slim et al. 2007), scorpion Scorpio maurus (Zouari et al. 2005), and squid Todaro ...
... To date, only a few studies are available about digestive lipases in crustaceans, their detailed function in lipid metabolism, and methods of purification and characterization. Lipases from crab Carcinus mediterraneus (Slim et al. 2007), scorpion Scorpio maurus (Zouari et al. 2005), and squid Todaro ...
Solution Structure of the Tandem Acyl Carrier Protein Domains from
... A sequence alignment of the five ACP domains shows how nearly identical these ACP domains are (Figure 4). With only 13 amino acid positions which are not absolutely conserved for all five ACP domains, it is not entirely apparent why the first ACP domain would not have been recognized as such in a BL ...
... A sequence alignment of the five ACP domains shows how nearly identical these ACP domains are (Figure 4). With only 13 amino acid positions which are not absolutely conserved for all five ACP domains, it is not entirely apparent why the first ACP domain would not have been recognized as such in a BL ...
Patterns, motifs, PSI-BLAST
... • Such automatic patterns are usually distinct from those designed by an expert with some knowledge of the biochemical literature. ...
... • Such automatic patterns are usually distinct from those designed by an expert with some knowledge of the biochemical literature. ...
Marine Biotecnology
... To date, only a few studies are available about digestive lipases in crustaceans, their detailed function in lipid metabolism, and methods of purification and characterization. Lipases from crab Carcinus mediterraneus (Slim et al. 2007), scorpion Scorpio maurus (Zouari et al. 2005), and squid Todaro ...
... To date, only a few studies are available about digestive lipases in crustaceans, their detailed function in lipid metabolism, and methods of purification and characterization. Lipases from crab Carcinus mediterraneus (Slim et al. 2007), scorpion Scorpio maurus (Zouari et al. 2005), and squid Todaro ...
Data mining for important amino acid residues in multiple sequence
... Enzymes are highly efficient bio-catalysts interesting for industries and medicine. Therefore, a goal of utmost importance in biochemical research is to understand how an enzyme catalyzes a chemical reaction. Here, the computational identification of functionally or structurally important residue po ...
... Enzymes are highly efficient bio-catalysts interesting for industries and medicine. Therefore, a goal of utmost importance in biochemical research is to understand how an enzyme catalyzes a chemical reaction. Here, the computational identification of functionally or structurally important residue po ...
Early events in protein folding
... usually taken as a signature of barrier-less folding51–53. It should, however, be noted that downhill folding reactions may also show exponential kinetics, depending on the roughness of the free energy surface54,55. Some mutant variants of the λ-repressor fragment, λ6-85, fold on a timescale close t ...
... usually taken as a signature of barrier-less folding51–53. It should, however, be noted that downhill folding reactions may also show exponential kinetics, depending on the roughness of the free energy surface54,55. Some mutant variants of the λ-repressor fragment, λ6-85, fold on a timescale close t ...
Recycling of vitamin B12 and NAD+ within the Pdu
... Recent crystallography studies suggested that some Pdu MCP shell proteins, such as PduA, T and U, have pores that may mediate the transport of enzyme substrates/cofactors across the MCP shell. However, it’s possible that the cofactors might be regenerated in the local vicinity of the metabolic enzym ...
... Recent crystallography studies suggested that some Pdu MCP shell proteins, such as PduA, T and U, have pores that may mediate the transport of enzyme substrates/cofactors across the MCP shell. However, it’s possible that the cofactors might be regenerated in the local vicinity of the metabolic enzym ...
The Vitamin K-dependent Carboxylase*
... protein is then secreted and circulates in the blood as a zymogen. The importance of the removal of the pro sequence for the normal function of a VKD blood protein is exemplified by the identification of Hemophilia B patients that have a point mutation at one of these conserved residues (e. g. R –4 ...
... protein is then secreted and circulates in the blood as a zymogen. The importance of the removal of the pro sequence for the normal function of a VKD blood protein is exemplified by the identification of Hemophilia B patients that have a point mutation at one of these conserved residues (e. g. R –4 ...
Basic Science for Clinicians
... gatekeeper, regulating the entry of glucose into glycolysis. However, the molecular signaling role of AMP is dramatically extended by activation of the AMPK pathway. Because many more proteins are regulated by AMPK than contain specialized AMP-binding sites, the ability of AMP to signal energy compr ...
... gatekeeper, regulating the entry of glucose into glycolysis. However, the molecular signaling role of AMP is dramatically extended by activation of the AMPK pathway. Because many more proteins are regulated by AMPK than contain specialized AMP-binding sites, the ability of AMP to signal energy compr ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic enzymes from the same organism, seem to be proteins. Some microorganisms, however, produce and secrete small non-proteinaceous inhibitors, which impair the proteolytic activity of host pe ...
... Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic enzymes from the same organism, seem to be proteins. Some microorganisms, however, produce and secrete small non-proteinaceous inhibitors, which impair the proteolytic activity of host pe ...
Multiple sequence alignment for phylogenetic purposes
... zone’ of protein similarity, where sequence alignment can be very problematic. The two most different sequences, human hydroxyacylglutathione hydrolase (or glyoxalase II) (labelled 1qh5A in the figure) and a bacterial penicillinase (labelled 1smlA), have only 16% amino-acid identity. Needless to say, ...
... zone’ of protein similarity, where sequence alignment can be very problematic. The two most different sequences, human hydroxyacylglutathione hydrolase (or glyoxalase II) (labelled 1qh5A in the figure) and a bacterial penicillinase (labelled 1smlA), have only 16% amino-acid identity. Needless to say, ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
... ACCase, and to characterize the biochemical properties of the CAC2 protein, we expressed it in E. coli (Fig. 2). An expressed GST-CAC2 fusion protein was used to generate antiserum (Fig. 2A). The resulting antiserum was used on western blots to identify the CAC2 protein in Arabidopsis leaf extracts ...
... ACCase, and to characterize the biochemical properties of the CAC2 protein, we expressed it in E. coli (Fig. 2). An expressed GST-CAC2 fusion protein was used to generate antiserum (Fig. 2A). The resulting antiserum was used on western blots to identify the CAC2 protein in Arabidopsis leaf extracts ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
... variants with greater specific activity, lower Km for peroxide, and higher thermostability. Starting with a mutant that is functionally expressed in Saccharomyces cerevisiae, we used random mutagenesis, recombination, and screening to identify HRP-C mutants that are more active and stable to incubat ...
... variants with greater specific activity, lower Km for peroxide, and higher thermostability. Starting with a mutant that is functionally expressed in Saccharomyces cerevisiae, we used random mutagenesis, recombination, and screening to identify HRP-C mutants that are more active and stable to incubat ...
The Cutting Edge of Affinity Electrophoresis Technology
... compound and a polysaccharide; (B) the affinity probe MPBA. 5. Supported Molecular Matrix Electrophoresis and Its Application to Affinity Electrophoresis Mucins are viscous glycoproteins produced by epithelial cells. It has been reported that changes in the structures of the sugar chains of mucins a ...
... compound and a polysaccharide; (B) the affinity probe MPBA. 5. Supported Molecular Matrix Electrophoresis and Its Application to Affinity Electrophoresis Mucins are viscous glycoproteins produced by epithelial cells. It has been reported that changes in the structures of the sugar chains of mucins a ...
IDENTIFICATION AND CHARACTERIZATION OF SYNAPTONEMAL
... arealigned at adistance of about 300 nm, aphenomenon called presynaptic alignment (from J.H. deJong, with permission). ...
... arealigned at adistance of about 300 nm, aphenomenon called presynaptic alignment (from J.H. deJong, with permission). ...
Comparison of Ligand-Binding Sites of Modeled Apo[a] Kringle
... little is known about the mechanism(s) by which Lp[a] leads to atherosclerosis or whether an elevated level of plasma Lp[a] is a cause or a result of atherosclerosis. Lp[a] contains two major and distinctly different highmolecular-weight apoproteins.5-7 Within Lp[a], apoprotein[a], apo[a], a highly ...
... little is known about the mechanism(s) by which Lp[a] leads to atherosclerosis or whether an elevated level of plasma Lp[a] is a cause or a result of atherosclerosis. Lp[a] contains two major and distinctly different highmolecular-weight apoproteins.5-7 Within Lp[a], apoprotein[a], apo[a], a highly ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748
... database, we found expressed sequence tag sequences of SGLT2 in the cDNA library of bovine mammary tissues, indicating its expression in bovine mammary gland. To facilitate study of the mechanism of glucose reabsorption in bovine kidneys in maintenance of glucose homeostasis of lactating cows and th ...
... database, we found expressed sequence tag sequences of SGLT2 in the cDNA library of bovine mammary tissues, indicating its expression in bovine mammary gland. To facilitate study of the mechanism of glucose reabsorption in bovine kidneys in maintenance of glucose homeostasis of lactating cows and th ...
Comparative Sequence Analysis of the Genomic Segment 6 of Four
... RNA encodes a protein of" 397 amino acids in all strains with the exception of strain H-2 in which it encodes a protein of 399 amino acids. Alignment of amino acid sequences of the VP6 protein of strain FI-14 and subgroup II rotaviruses (Wa and Gottfried) indicates a high degree of homology (94~), w ...
... RNA encodes a protein of" 397 amino acids in all strains with the exception of strain H-2 in which it encodes a protein of 399 amino acids. Alignment of amino acid sequences of the VP6 protein of strain FI-14 and subgroup II rotaviruses (Wa and Gottfried) indicates a high degree of homology (94~), w ...
The Three-Dimensional Structure of Aspergillus niger Pectin Lyase
... preparations. Pectin lyases cleave highly esterified pectins without affecting the volatile esters of scent as well as reduce flocculating effects due to fruit Ca21 complexes if de-esterified pectin derivatives are present (Fogarty and Kelly, 1982; Alana et al., 1989). PLB belongs to a multigene fam ...
... preparations. Pectin lyases cleave highly esterified pectins without affecting the volatile esters of scent as well as reduce flocculating effects due to fruit Ca21 complexes if de-esterified pectin derivatives are present (Fogarty and Kelly, 1982; Alana et al., 1989). PLB belongs to a multigene fam ...
The Structure of the PanD/PanZ Protein Complex
... of enzymes have been shown to be enhanced by accessory proteins (McDonald et al., 1998), although the structural basis for such enhancement has not been determined. Since the first identification of pyruvoyl-dependent enzymes (van Poelje and Snell, 1990; Williamson and Brown, 1979), it has been wide ...
... of enzymes have been shown to be enhanced by accessory proteins (McDonald et al., 1998), although the structural basis for such enhancement has not been determined. Since the first identification of pyruvoyl-dependent enzymes (van Poelje and Snell, 1990; Williamson and Brown, 1979), it has been wide ...
Identification of a structural motif that confers specific interaction with
... To identify novel COP1-interacting proteins, we used fulllength COP1 protein fused to the Gal4 DNA-binding domain (Gal4-BD) to screen a library made from 3-dayold dark-grown Arabidopsis seedlings (Kim et al., 1997). Fourteen of ~170 000 independent transformants allowed auxotrophic growth and showed ...
... To identify novel COP1-interacting proteins, we used fulllength COP1 protein fused to the Gal4 DNA-binding domain (Gal4-BD) to screen a library made from 3-dayold dark-grown Arabidopsis seedlings (Kim et al., 1997). Fourteen of ~170 000 independent transformants allowed auxotrophic growth and showed ...