Slide 1
... The dataset for the algorithm for binding site prediction and extraction : 90 HIV protease protein (21 wild type, and 69 mutated) PDBs The descriptors such as volume, dipole moment, moment of inertia, quadruple moment, hydrophobicity, residue interface propensity, integral of properties, and, Betti ...
... The dataset for the algorithm for binding site prediction and extraction : 90 HIV protease protein (21 wild type, and 69 mutated) PDBs The descriptors such as volume, dipole moment, moment of inertia, quadruple moment, hydrophobicity, residue interface propensity, integral of properties, and, Betti ...
A new type of Hidden Markov Models to predict complex domain
... transitions between states. The core of a profile HMM (see the insert in Fig. 1) is a linear sequence of match (M ) states, one for each conserved position (consensus column) of a multiple alignment. Each M state emits (aligns to) a single residue, with a probability that is determined by the frequ ...
... transitions between states. The core of a profile HMM (see the insert in Fig. 1) is a linear sequence of match (M ) states, one for each conserved position (consensus column) of a multiple alignment. Each M state emits (aligns to) a single residue, with a probability that is determined by the frequ ...
From DNA to Protein: Transcription and Translation
... nucleus it goes to a ribosome (site of protein synthesis). ...
... nucleus it goes to a ribosome (site of protein synthesis). ...
In silico Prediction and Docking of Tertiary Structure of LuxI, an
... Background: LuxI is a component of the quorum sensing signaling pathway in Vibrio fischeri responsible for the inducer synthesis that is essential for bioluminescence. Methods: Homology modeling of LuxI was carried out using Phyre2 and refined with the GalaxyWEB server. Five models were generated an ...
... Background: LuxI is a component of the quorum sensing signaling pathway in Vibrio fischeri responsible for the inducer synthesis that is essential for bioluminescence. Methods: Homology modeling of LuxI was carried out using Phyre2 and refined with the GalaxyWEB server. Five models were generated an ...
L -2 Sample preparation Before crystallization (first step
... Is the sample pooled purification aliquots or a single batch? How much sample do I have and how much more is available? How pure is the sample? How homogeneous is the sample? Does anyone possess any solubility information on this sample? What is unique about this protein? What is necessary chemicall ...
... Is the sample pooled purification aliquots or a single batch? How much sample do I have and how much more is available? How pure is the sample? How homogeneous is the sample? Does anyone possess any solubility information on this sample? What is unique about this protein? What is necessary chemicall ...
没有幻灯片标题
... 2.4.2 All these proteins are involved in molecular recognition (antigen recognition by antibodies, TCR, and MHC proteins; cell-cell interactions by ICAMs). 2.4.3 Most of the immunoglobulin superfamily members are found on cell surfaces (e.g., IgM, TCR, MHC) or secreted (IgG). ...
... 2.4.2 All these proteins are involved in molecular recognition (antigen recognition by antibodies, TCR, and MHC proteins; cell-cell interactions by ICAMs). 2.4.3 Most of the immunoglobulin superfamily members are found on cell surfaces (e.g., IgM, TCR, MHC) or secreted (IgG). ...
Proteins in nutrition
... Adults – about 1,0 g proteins / kg of body weight / day Children: quick growth phase (till 2 years) : about 1,5 g / kg / day other: about 1,2 – 1,5 g / kg / day Pregnancy and breastfeeding: about 1,5 g / kg / day Sportsmen in training: max. 1,3 – 1,5 g / kg / day (higher amount do not use for proteo ...
... Adults – about 1,0 g proteins / kg of body weight / day Children: quick growth phase (till 2 years) : about 1,5 g / kg / day other: about 1,2 – 1,5 g / kg / day Pregnancy and breastfeeding: about 1,5 g / kg / day Sportsmen in training: max. 1,3 – 1,5 g / kg / day (higher amount do not use for proteo ...
STUDYING PROTEIN DYNAMICS USING NMR Martin
... measurements (see CvHeijenoort1.pdf). Further examples will be encountered in the afternoon practical session. Residual dipolar couplings (RDCs) have emerged as a powerful addition to these techniques. The sensitivity of RDCs to the relative orientation of a pair of coupled spins has led to their ro ...
... measurements (see CvHeijenoort1.pdf). Further examples will be encountered in the afternoon practical session. Residual dipolar couplings (RDCs) have emerged as a powerful addition to these techniques. The sensitivity of RDCs to the relative orientation of a pair of coupled spins has led to their ro ...
Mr. David Cortens In Vivo Synthesis of ?Click? Functionalized
... The site-specific modification of proteins plays an important role in current technologies. With the fast progress and miniaturization of protein-based applications, a controlled orientation of proteins on solid surfaces is required. Here we focus on the production of innovative, sensitive and cost- ...
... The site-specific modification of proteins plays an important role in current technologies. With the fast progress and miniaturization of protein-based applications, a controlled orientation of proteins on solid surfaces is required. Here we focus on the production of innovative, sensitive and cost- ...
Structural and functional analyses of a yeast mitochondrial
... also been found to have sequence similarity with S15-like proteins. Allowing for the insertion of five amino acids toward the carboxy-terminus of the archaebacterial protein, there is 24%, 17%, and 16% sequence identity with eubacterial, chloroplast, and mitochondrial S15-like proteins, respectively ...
... also been found to have sequence similarity with S15-like proteins. Allowing for the insertion of five amino acids toward the carboxy-terminus of the archaebacterial protein, there is 24%, 17%, and 16% sequence identity with eubacterial, chloroplast, and mitochondrial S15-like proteins, respectively ...
How to build a glycinergic postsynaptic ...
... genomic sequences encoding a fourth variant, a4, have been isolated from mouse (Y. Maulet, B. Matzenbach, and H. Betz, unpublished observations). All these a sequences display a high degree of amino acid identity and correspond to GlyR proteins, whose expression is under distinct temporal and region ...
... genomic sequences encoding a fourth variant, a4, have been isolated from mouse (Y. Maulet, B. Matzenbach, and H. Betz, unpublished observations). All these a sequences display a high degree of amino acid identity and correspond to GlyR proteins, whose expression is under distinct temporal and region ...
coIP就是co-immunoprecipitation，假如有两种蛋白质A,B相互作用
... disassemble in concert with an ever-changing influx of intra, inter and extracellular cues. A preliminary step in understanding protein structure and function is to determine which proteins interact with each other, thereby identifying the relevant biological pathways. The pull-down technique has be ...
... disassemble in concert with an ever-changing influx of intra, inter and extracellular cues. A preliminary step in understanding protein structure and function is to determine which proteins interact with each other, thereby identifying the relevant biological pathways. The pull-down technique has be ...
Illustration of Skeletal Muscle Calsequestrin Complex Formation by
... and two sarcoplasmic reticulum markers, the ryanodine receptor and triadin, were determined by indirect immunofluorescence microscopy [2]. Nuclei were identified by blue DAPI staining. The preparation of muscle tissues, the characterization of monoclonal antibodies, the description of materials used ...
... and two sarcoplasmic reticulum markers, the ryanodine receptor and triadin, were determined by indirect immunofluorescence microscopy [2]. Nuclei were identified by blue DAPI staining. The preparation of muscle tissues, the characterization of monoclonal antibodies, the description of materials used ...
CS273_SequenceSimilarity1
... Actin sequence • Actin is ancient and abundant Most abundant protein in cells 1-2 actin genes in bacteria, yeasts, amoebas ...
... Actin sequence • Actin is ancient and abundant Most abundant protein in cells 1-2 actin genes in bacteria, yeasts, amoebas ...
The Case Against a Darwinian Origin of Protein Folds
... that these long proteins perform an impressive variety of functions with equally impressive specificity and efficiency. In the face of this, either we accept that proteins are what they seem to be—long amino acid chains that need to meet stringent sequence requirements in order to work—or we suppose ...
... that these long proteins perform an impressive variety of functions with equally impressive specificity and efficiency. In the face of this, either we accept that proteins are what they seem to be—long amino acid chains that need to meet stringent sequence requirements in order to work—or we suppose ...
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families
... measure of similarity on the presence or absence of motifs that are likely to have functional relevance. While there are extensive databases of known patterns and profiles that have been built from alignments of related sequences (Mulder and Apweiler 2001), novel sets of statistically significant mo ...
... measure of similarity on the presence or absence of motifs that are likely to have functional relevance. While there are extensive databases of known patterns and profiles that have been built from alignments of related sequences (Mulder and Apweiler 2001), novel sets of statistically significant mo ...
Experimentally testing the hypothesis of a limited amino acid
... As to the origin and evolution of the genetic code, a number of theories have been proposed. Crick proposed the frozen accident theory [1] in which he suggested two things; i) The code is universal because, at the present time, any change would be lethal, or at least very strongly selected against; ...
... As to the origin and evolution of the genetic code, a number of theories have been proposed. Crick proposed the frozen accident theory [1] in which he suggested two things; i) The code is universal because, at the present time, any change would be lethal, or at least very strongly selected against; ...
INSILICO MODELING OF CAPSULAR POLYSACCHARIDE BIOSYNTHESIS PROTEIN STREPTOCOCCUS PNEUMONIAE LIGAND IDENTIFICATION
... using MODELLER 9v7 and validated using Structural Analysis and Verification Server. Ligands were taken from drug port and its analogues were retrieved from pubchem. These ligands were docked to the modeled proteins using DISCOVERY STUDIO and the ADMET toxicity of the ligands was also studied. Result ...
... using MODELLER 9v7 and validated using Structural Analysis and Verification Server. Ligands were taken from drug port and its analogues were retrieved from pubchem. These ligands were docked to the modeled proteins using DISCOVERY STUDIO and the ADMET toxicity of the ligands was also studied. Result ...
Cloning, sequence and in vitro transcription/translation analysis of a
... beginning of ORF I (.5’-GTATGAGT) and the 1-nt overlap between the end of ORF 1 and the beginning of ORF E (5’-CTTAATGGC) suggest possible translational coupling of these ORFs during translation from a single polycistronic transcript (Normark et al.. 1983) derived from an AIE operon. The presence of ...
... beginning of ORF I (.5’-GTATGAGT) and the 1-nt overlap between the end of ORF 1 and the beginning of ORF E (5’-CTTAATGGC) suggest possible translational coupling of these ORFs during translation from a single polycistronic transcript (Normark et al.. 1983) derived from an AIE operon. The presence of ...
Molecular and General Genetics
... the B. amyloliquefaciens and the similar B. subtilis betaglucanases, but striking homologies between those mesophilic enzymes and thermophilic B. macerans beta-glucanase do exist. The extent of similarity of amino acid sequence among the beta-glucanases of B. macerans and B. amyloliquefaciens is 70% ...
... the B. amyloliquefaciens and the similar B. subtilis betaglucanases, but striking homologies between those mesophilic enzymes and thermophilic B. macerans beta-glucanase do exist. The extent of similarity of amino acid sequence among the beta-glucanases of B. macerans and B. amyloliquefaciens is 70% ...
bodybuilding supplements
... are controlled substances in many countries, including the USA and the UK, while in other countries they are available over-the-counter. In countries where steroids are controlled, there is often a black market in which smuggled or counterfeit drugs are sold to users. The quality of these illegal dr ...
... are controlled substances in many countries, including the USA and the UK, while in other countries they are available over-the-counter. In countries where steroids are controlled, there is often a black market in which smuggled or counterfeit drugs are sold to users. The quality of these illegal dr ...
Mutation of exposed hydrophobic amino acids to arginine to
... were expressed with the baculovirus system [29]. This enzyme is a dimer anchor to the membrane via a glycolipid [30]. We expressed a soluble dimeric form obtained by deletion of the hydrophobic peptide, precursor of the glycolipid anchor at the C-terminal end [31]. Furthermore, the enzyme was delete ...
... were expressed with the baculovirus system [29]. This enzyme is a dimer anchor to the membrane via a glycolipid [30]. We expressed a soluble dimeric form obtained by deletion of the hydrophobic peptide, precursor of the glycolipid anchor at the C-terminal end [31]. Furthermore, the enzyme was delete ...
2nd Amino Acid Workshop - Maastricht Proteomics Center
... electrophoresis (11,12). In the latter approach, a sample containing a mixture of proteins is applied to a gel to which ampholytes are added in a way that divides the surface of the gel into a defined pH-range. An electric current focuses the different proteins to a band on the gel according to thei ...
... electrophoresis (11,12). In the latter approach, a sample containing a mixture of proteins is applied to a gel to which ampholytes are added in a way that divides the surface of the gel into a defined pH-range. An electric current focuses the different proteins to a band on the gel according to thei ...
Nucleotide sequence of the 3h-terminal two
... outgroup. In the case of HSP70, the pea HSP70 (L03299) was used as a non-closterovirus outgroup. In the case of CP, sweet potato sunken vein virus (SPSVV) CP (X80995) was also included. To facilitate the alignment, only 250 amino acid residues in the C-terminal portion of CP (LChV and GLRaV-3) and C ...
... outgroup. In the case of HSP70, the pea HSP70 (L03299) was used as a non-closterovirus outgroup. In the case of CP, sweet potato sunken vein virus (SPSVV) CP (X80995) was also included. To facilitate the alignment, only 250 amino acid residues in the C-terminal portion of CP (LChV and GLRaV-3) and C ...