Dot plot
... • What is the function of P05049? • P05049 is a serine protease. Would you run a wet lab experiment ...
... • What is the function of P05049? • P05049 is a serine protease. Would you run a wet lab experiment ...
Complete Mitochondrial DNA Sequence and Amino Acid Analysis of
... reported in the literature are numerous; therefore several sequences were selected that provide a broad representation of the different insect genera and these sequences were aligned with AeCOI using ClustalW (Thompson et al., 1994). Sequence alignment reveals that 12 amino acid residues are unique ...
... reported in the literature are numerous; therefore several sequences were selected that provide a broad representation of the different insect genera and these sequences were aligned with AeCOI using ClustalW (Thompson et al., 1994). Sequence alignment reveals that 12 amino acid residues are unique ...
Cdc45: the missing RecJ ortholog in eukaryotes?
... Fig. 1. Sequence analysis of the Cdc45 and RecJ protein families. (A) Domain architecture of human Cdc45 and Escherichia coli RecJ proteins. For the E.coli RecJ protein, domains were assigned according to the RecJ core structure (Yamagata et al., 2002) and the Pfam domain database (Finn et al., 2008 ...
... Fig. 1. Sequence analysis of the Cdc45 and RecJ protein families. (A) Domain architecture of human Cdc45 and Escherichia coli RecJ proteins. For the E.coli RecJ protein, domains were assigned according to the RecJ core structure (Yamagata et al., 2002) and the Pfam domain database (Finn et al., 2008 ...
Document
... column) while gij are the frequencies observed in a database (e.g., the complete alignment, sequence family, or complete sequence database) • and are scaling factors to weigh the f and g contributions: if equals the number of sequences in profile, and =1 (preceding slide), then the influence ...
... column) while gij are the frequencies observed in a database (e.g., the complete alignment, sequence family, or complete sequence database) • and are scaling factors to weigh the f and g contributions: if equals the number of sequences in profile, and =1 (preceding slide), then the influence ...
Slide 1
... (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help ident ...
... (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help ident ...
ProSEC 300S
... shape and are generally soluble in water. Nearly all enzymes with major metabolic functions are globular proteins. ...
... shape and are generally soluble in water. Nearly all enzymes with major metabolic functions are globular proteins. ...
Domain fusion between SNF1-related kinase subunits during plant
... (Niogret et al., 1996). These experiments identified a novel protein similar to yeast and animal SNF4/AMPKγ factors that we have named AKINβγ-1. Although a functional interaction of AKINβγ-1 with Rab28 has not been confirmed yet, full-length cDNA clones were characterized leading to the identificati ...
... (Niogret et al., 1996). These experiments identified a novel protein similar to yeast and animal SNF4/AMPKγ factors that we have named AKINβγ-1. Although a functional interaction of AKINβγ-1 with Rab28 has not been confirmed yet, full-length cDNA clones were characterized leading to the identificati ...
Improving protein fold recognition with hybrid
... the most challenging questions in science (Kennedy and Norman, 2005). The resolution of this problem would have a great impact on various fields of biology and medicine, allowing, for example, the rational design of new protein functions and drug molecules. Despite the progress made over the last de ...
... the most challenging questions in science (Kennedy and Norman, 2005). The resolution of this problem would have a great impact on various fields of biology and medicine, allowing, for example, the rational design of new protein functions and drug molecules. Despite the progress made over the last de ...
B2 - Enzymes
... B2 - Enzymes Starter: Which of these uses enzymes? Answer: Photosynthesis, digestion, respiration and biological washing powders all use enzymes! ...
... B2 - Enzymes Starter: Which of these uses enzymes? Answer: Photosynthesis, digestion, respiration and biological washing powders all use enzymes! ...
Structural Bioinformatics
... • Pioneered by David Baker’s group in the late 1990s • Remarkable success in CASP and CAFASP experiments • Recently made publicly available on an automated server by Christopher Bystroff’s group • Pot pourri of many different approaches • Key components – ‘Divide and conquer’ strategy with respect t ...
... • Pioneered by David Baker’s group in the late 1990s • Remarkable success in CASP and CAFASP experiments • Recently made publicly available on an automated server by Christopher Bystroff’s group • Pot pourri of many different approaches • Key components – ‘Divide and conquer’ strategy with respect t ...
Human Saliva Amylase Alpha
... 590 Units/mg Protein.One unit will catalyze the hydrolysis of one micromole of malto pentaose, which through coupled reactions results in the formation of 5 micromoles of glucose-6-phosphate per minute at 37°C. Measured at 340nm as an equimolar amount of NADH produced by a coupled reaction. ...
... 590 Units/mg Protein.One unit will catalyze the hydrolysis of one micromole of malto pentaose, which through coupled reactions results in the formation of 5 micromoles of glucose-6-phosphate per minute at 37°C. Measured at 340nm as an equimolar amount of NADH produced by a coupled reaction. ...
A Study of Phylogenetic Relationships and Homology of
... unlikely to survive. [Margoliash E, 1963]. Hence, the relationship between organisms can be compared by examining the amino acid sequence of cyt c. A protein can be assessed comprehensively by comparing its structure, function and sequence. The similarities or differences in these three criteria are ...
... unlikely to survive. [Margoliash E, 1963]. Hence, the relationship between organisms can be compared by examining the amino acid sequence of cyt c. A protein can be assessed comprehensively by comparing its structure, function and sequence. The similarities or differences in these three criteria are ...
A gene fusion consisting of 960 base pairs of 5`
... a-factor precursor (and other) proteins such that the secreted interferon molecules are free of most or all of a-factor prepro sequence. Emr et _al_. (31) reached a similar conclusion by studying a fusion between the MFal gene and the yeast SUC2 gene. They found that active invertase was secreted in ...
... a-factor precursor (and other) proteins such that the secreted interferon molecules are free of most or all of a-factor prepro sequence. Emr et _al_. (31) reached a similar conclusion by studying a fusion between the MFal gene and the yeast SUC2 gene. They found that active invertase was secreted in ...
Richards, F.M. The Protein Folding Problem. Scientific American, pp
... genes.) Outside factors, such as en- acids. The relative importance of varizymes that might catalyze folding, did ous rules of folding and assembly may not have to be invoked as mandatory be somewhat different for those proteins than for others-notably long fiparticipants. The discovery. which has s ...
... genes.) Outside factors, such as en- acids. The relative importance of varizymes that might catalyze folding, did ous rules of folding and assembly may not have to be invoked as mandatory be somewhat different for those proteins than for others-notably long fiparticipants. The discovery. which has s ...
Bacteriophage lambda surface display of a bacterial biotin acceptor
... biotinylation in E. coli. Stepwise truncations were also carried out on Ps_TC [23]. Removal of the last amino acid had no e¡ect, whereas removal of two amino acids from the C-terminus completely abolished biotinylation. Their ¢nding is in absolute agreement with the C-terminus of the minimal biotiny ...
... biotinylation in E. coli. Stepwise truncations were also carried out on Ps_TC [23]. Removal of the last amino acid had no e¡ect, whereas removal of two amino acids from the C-terminus completely abolished biotinylation. Their ¢nding is in absolute agreement with the C-terminus of the minimal biotiny ...
Comparative study of pathogenesis-related protein - NOPR
... the homology was in the range 75.67 (AlPR5) to 91.92% (ZoPR5-1). However, the maximum homology for ZzPR5 (85.71%) was observed with ZzPR5-1 and the minimum with AlPR5 (66.21%). The different physical and chemical protein parameters for PR5 proteins were analysed using the Expasy Protparam tool13 and ...
... the homology was in the range 75.67 (AlPR5) to 91.92% (ZoPR5-1). However, the maximum homology for ZzPR5 (85.71%) was observed with ZzPR5-1 and the minimum with AlPR5 (66.21%). The different physical and chemical protein parameters for PR5 proteins were analysed using the Expasy Protparam tool13 and ...
• 100 times faster than dynamic programming. • Good for database
... – The E-value depends on the size of database and the scoring system in use use. – When the E-value threshold is increased from the default value of 10, more hits can be reported. When reduced, more significant hits are reported. – The lower the E E-value value (or higher the bit score), the more si ...
... – The E-value depends on the size of database and the scoring system in use use. – When the E-value threshold is increased from the default value of 10, more hits can be reported. When reduced, more significant hits are reported. – The lower the E E-value value (or higher the bit score), the more si ...
Chapter 2.3: Proteins
... • Polypeptide chains may contain several hundred amino acids linked by peptide bonds • The particular amino acids and their ORDER in the sequence is called the primary structure of the protein ...
... • Polypeptide chains may contain several hundred amino acids linked by peptide bonds • The particular amino acids and their ORDER in the sequence is called the primary structure of the protein ...
Sequences of the Nucleocapsid Genes from Two Strains of Avian
... cDNA chining. Three cDNA clones, derived from RNA isolated from gradient-purified virus, have been used for sequencing. For Beaudene the oligo(dT)-primed clone C5.322 was used, which includes the poly(A) tail derived from the 3' terminus of the viral genome and extends for 1930base pairs in the 5' d ...
... cDNA chining. Three cDNA clones, derived from RNA isolated from gradient-purified virus, have been used for sequencing. For Beaudene the oligo(dT)-primed clone C5.322 was used, which includes the poly(A) tail derived from the 3' terminus of the viral genome and extends for 1930base pairs in the 5' d ...
Robustness of the model
... Robustness of the model In the model for emulating the history of the centrosome, there are a number of choices: Which protein classes make up the structural backbone? Is it necessary to assume a structural backbone? In this section, we show that coiled-coil proteins are unique among the protein cla ...
... Robustness of the model In the model for emulating the history of the centrosome, there are a number of choices: Which protein classes make up the structural backbone? Is it necessary to assume a structural backbone? In this section, we show that coiled-coil proteins are unique among the protein cla ...
Protein Modeling Challenge Science Olympiad Trial Event
... Protein folding is due to the behavior of different chemical groups on amino acids in an aqueous environment Explore amino acid sidechain chemistry and protein folding with the Amino Acid Starter Kit Activity (ITOPS, Introduction To Protein Structure) (www) ...
... Protein folding is due to the behavior of different chemical groups on amino acids in an aqueous environment Explore amino acid sidechain chemistry and protein folding with the Amino Acid Starter Kit Activity (ITOPS, Introduction To Protein Structure) (www) ...