Database Modeling in Bioinformatics
... • Multifunctional proteins- genome projects often assign single function, info is lost in homology search • Hypothetical proteins (40% oRFs unknown), and poorly or even wrongly annotated proteins • No coverage of position-specific annotation eg active sites • Current methods provide only a phrase de ...
... • Multifunctional proteins- genome projects often assign single function, info is lost in homology search • Hypothetical proteins (40% oRFs unknown), and poorly or even wrongly annotated proteins • No coverage of position-specific annotation eg active sites • Current methods provide only a phrase de ...
Microarray on Germinating Yeast Spores (WP2)
... What is/are the environmental sensor(s) and the signal transduction pathway(s) involved in yeast spore germination? ...
... What is/are the environmental sensor(s) and the signal transduction pathway(s) involved in yeast spore germination? ...
Membrane pore architecture of the CslF6
... membrane proteins with a cytoplasmic active site and a membrane pore through which the polysaccharides are transported to exit the cell (1). The biosynthetic genes belong to the cellulose synthase (CesA) (2) and cellulose synthase–like (Csl) gene families (3). The CslF6 protein is a major component ...
... membrane proteins with a cytoplasmic active site and a membrane pore through which the polysaccharides are transported to exit the cell (1). The biosynthetic genes belong to the cellulose synthase (CesA) (2) and cellulose synthase–like (Csl) gene families (3). The CslF6 protein is a major component ...
Retinoic Acid and the Development of the Endoderm
... knockouts in mice that CRABPs and CRBP are not essential proteins [62,63]. Thus, the requirement for these binding proteins in intercellular signaling by RA is not well understood, although a role for CRABPs in hindbrain patterning in zebrafish, presumably through altering the dynamics of the retino ...
... knockouts in mice that CRABPs and CRBP are not essential proteins [62,63]. Thus, the requirement for these binding proteins in intercellular signaling by RA is not well understood, although a role for CRABPs in hindbrain patterning in zebrafish, presumably through altering the dynamics of the retino ...
Project proposal paper - igem
... inducible receptor is a fusion of the light responsive domain, Cph1 (maximally responsive at 660nm), and the Histidine-kinase domain, EnvZ (capable of phosphorylating the transcription factor OmpR) (1). Additional accessory proteins required for formation of the receptor are also present on the devi ...
... inducible receptor is a fusion of the light responsive domain, Cph1 (maximally responsive at 660nm), and the Histidine-kinase domain, EnvZ (capable of phosphorylating the transcription factor OmpR) (1). Additional accessory proteins required for formation of the receptor are also present on the devi ...
Amino acids and insulin act additively to regulate components of the
... Results: Incubation of C2C12 myotubes with 0.2 × physiological amino acids concentration (0.2 × PC AA), relative to 1.0 × PC AA, significantly increased total proteolysis and the expression of 14-kDa E2 ubiquitin conjugating enzyme (p < 0.05). The proteasome inhibitor MG132 blocked the rise in prote ...
... Results: Incubation of C2C12 myotubes with 0.2 × physiological amino acids concentration (0.2 × PC AA), relative to 1.0 × PC AA, significantly increased total proteolysis and the expression of 14-kDa E2 ubiquitin conjugating enzyme (p < 0.05). The proteasome inhibitor MG132 blocked the rise in prote ...
CD - Instituto de Investigaciones Biotecnológicas
... Radar - De novo repeat detection in protein sequences REP - Searches a protein sequence for repeats REPRO - De novo repeat detection in protein sequences TRUST - De novo repeat detection in protein sequences XSTREAM - De novo tandem repeat detection and architecture modeling in protein sequences SAP ...
... Radar - De novo repeat detection in protein sequences REP - Searches a protein sequence for repeats REPRO - De novo repeat detection in protein sequences TRUST - De novo repeat detection in protein sequences XSTREAM - De novo tandem repeat detection and architecture modeling in protein sequences SAP ...
Glycolysis is the major oxidative pathway for glucose
... Glucose uptake from blood Blood & cells (either direction) Fructose transport ...
... Glucose uptake from blood Blood & cells (either direction) Fructose transport ...
6. Protein Folding
... proline peptides is a slow process and in vitro it is frequently the rate limiting step in folding for those molecules that have been trapped in a folding intermediate with wrong isomers. ...
... proline peptides is a slow process and in vitro it is frequently the rate limiting step in folding for those molecules that have been trapped in a folding intermediate with wrong isomers. ...
lecture11&12-RS_Major Metabolic Pathways of
... Glucose uptake from blood Blood & cells (either direction) Fructose transport ...
... Glucose uptake from blood Blood & cells (either direction) Fructose transport ...
Chapter 16 Glycolysis and gluconeogenesis
... F1,6bisP F6P G6P ••• Glc (Does not take place in cytoplasm) The endpoint of gluconeogenesis in most tissues, can keep Glc or G6P is converted into glycogen. In liver and to a lesser extent the kidney, five proteins are involved ...
... F1,6bisP F6P G6P ••• Glc (Does not take place in cytoplasm) The endpoint of gluconeogenesis in most tissues, can keep Glc or G6P is converted into glycogen. In liver and to a lesser extent the kidney, five proteins are involved ...
An LL-Diaminopimelate Aminotransferase
... LL-DAP-AT activity. None of the other genes produced such an activity. At4g33680 was annotated as a 461-amino acid, class I/II family aminotransferase. The first 36 amino acids were predicted by TargetP to be a transit peptide for localization of the protein to plastids. The closest paralog to At4g3 ...
... LL-DAP-AT activity. None of the other genes produced such an activity. At4g33680 was annotated as a 461-amino acid, class I/II family aminotransferase. The first 36 amino acids were predicted by TargetP to be a transit peptide for localization of the protein to plastids. The closest paralog to At4g3 ...
Pharmacolecture 12 - pharmacology1lecnotes
... Histamine is released from mast cells and basophils by two general processes of degranulation: noncytolytic and cytolytic. Cytolytic release of histamine from mast cells occurs when the plasma membrane is damaged. This type of release is energy- independent, does not require intracellular Ca2+, and ...
... Histamine is released from mast cells and basophils by two general processes of degranulation: noncytolytic and cytolytic. Cytolytic release of histamine from mast cells occurs when the plasma membrane is damaged. This type of release is energy- independent, does not require intracellular Ca2+, and ...
Conservation of the three-dimensional structure in non
... consisting of six alpha helices [12]. The number of helices can be altered in different families of globin-like proteins. These helices are not randomly distributed in the protein, but they are oriented following standard helixhelix packing rules in order to form a globular structure. Globin-like fo ...
... consisting of six alpha helices [12]. The number of helices can be altered in different families of globin-like proteins. These helices are not randomly distributed in the protein, but they are oriented following standard helixhelix packing rules in order to form a globular structure. Globin-like fo ...
Identification of the P2Y12 Receptor in Nucleotide Inhibition of
... which multiple P2 receptors may be activated, stimulation of P1 adenosine receptors after breakdown of purines by ectonucleotidases, and the limited availability of P2 receptor subtype-selective agonists and antagonists. Adrenal chromaffin cells are embryonically derived from precursors of sympathet ...
... which multiple P2 receptors may be activated, stimulation of P1 adenosine receptors after breakdown of purines by ectonucleotidases, and the limited availability of P2 receptor subtype-selective agonists and antagonists. Adrenal chromaffin cells are embryonically derived from precursors of sympathet ...
Developing a Novel Means of Observing the
... Axonogenesis- the growth of axons- is an essential component of development for all organisms with a functioning nervous system. Once the process is complete, the axons, or nerve fibers, allow signals to be transmitted from one part of the body to the next in response to a series of chemical changes ...
... Axonogenesis- the growth of axons- is an essential component of development for all organisms with a functioning nervous system. Once the process is complete, the axons, or nerve fibers, allow signals to be transmitted from one part of the body to the next in response to a series of chemical changes ...
a proprietary enzyme blend designed
... Increased leucine, glucose and phosphatidic acid concentrations all activate mTOR, which subsequently activates protein synthesis and increases a cell’s capacity to produce new proteins.19-21 Ingestion of leucine independent of exercise has been shown to increase the phosphorylation of mTOR (mTOR ac ...
... Increased leucine, glucose and phosphatidic acid concentrations all activate mTOR, which subsequently activates protein synthesis and increases a cell’s capacity to produce new proteins.19-21 Ingestion of leucine independent of exercise has been shown to increase the phosphorylation of mTOR (mTOR ac ...
H - IS MU
... Laktose synthase is a complex of two proteins: • galactosyl transferase (present in many tissues) • -lactalbumin (present only in mammary gland during lactation, the synthesis is stimulated by hormone prolactin) ...
... Laktose synthase is a complex of two proteins: • galactosyl transferase (present in many tissues) • -lactalbumin (present only in mammary gland during lactation, the synthesis is stimulated by hormone prolactin) ...
Nitrogen lectures (part 3)
... » Rapid analysis is difficult and expensive – Animal requirements » Differ between controlled research and in the field » Requirements vary with: Animal genetics Sex Stage of growth Environmental temperature Balance with other nutrients Growth promotants – Ractopamine (Paylean) ...
... » Rapid analysis is difficult and expensive – Animal requirements » Differ between controlled research and in the field » Requirements vary with: Animal genetics Sex Stage of growth Environmental temperature Balance with other nutrients Growth promotants – Ractopamine (Paylean) ...
regionally specific induction by the spemann
... group of cells in vertebrate embryos, the morphogenetic and inductive properties of which are of paramount importance for the early establishment of the vertebrate body plan. Grafted to the ventral side of a host embryo, the DORSAL BLASTOPORE LIP, which corresponds to the organizer in amphibians, in ...
... group of cells in vertebrate embryos, the morphogenetic and inductive properties of which are of paramount importance for the early establishment of the vertebrate body plan. Grafted to the ventral side of a host embryo, the DORSAL BLASTOPORE LIP, which corresponds to the organizer in amphibians, in ...
A Comparison of Suffix Tree based Indexing and Search
... a protein structure in a database containing all the known proteins are needed to classify the protein and predict its function. Comparing the structure of unknown protein individually with every protein in the database can be highly inefficient. Database indexing methods are therefore best suited f ...
... a protein structure in a database containing all the known proteins are needed to classify the protein and predict its function. Comparing the structure of unknown protein individually with every protein in the database can be highly inefficient. Database indexing methods are therefore best suited f ...
The Crystal Structure of Arabidopsis thaliana Allene Oxide Cyclase
... In complete agreement with our biochemical data, we found AOC2 to form trimers when crystallized (Figure 4). The barrel axes of the monomers are tilted ;308 with respect to the trimer axis and pack closely with their barrel walls. Trimerization buries ;2000 A2 of the barrel surface and is the only i ...
... In complete agreement with our biochemical data, we found AOC2 to form trimers when crystallized (Figure 4). The barrel axes of the monomers are tilted ;308 with respect to the trimer axis and pack closely with their barrel walls. Trimerization buries ;2000 A2 of the barrel surface and is the only i ...
A consensus sequence in the endothelin
... increases NHE3 activity (7). In addition, NHE3 activation by ET-1 was inhibited by ETB receptor blockers, but not by ETA receptor blockers. Finally, in proximal tubules harvested from ETB receptor knockout mice, ET-1 fails to increase NHE3 activity, whereas stimulation is seen in tubules from wild-t ...
... increases NHE3 activity (7). In addition, NHE3 activation by ET-1 was inhibited by ETB receptor blockers, but not by ETA receptor blockers. Finally, in proximal tubules harvested from ETB receptor knockout mice, ET-1 fails to increase NHE3 activity, whereas stimulation is seen in tubules from wild-t ...
Subunit Isoform of X,K-ATPase in Human Skeletal Muscle
... N-terminal hexahistidine tag. Only variant C was successfully cloned into that vector (resulting construct pH3130) while no recombinant colonies were obtained for variants A and B. This is possibly due to their severe toxicity to bacteria (a frequent phenomenon for recombinant proteins with hydropho ...
... N-terminal hexahistidine tag. Only variant C was successfully cloned into that vector (resulting construct pH3130) while no recombinant colonies were obtained for variants A and B. This is possibly due to their severe toxicity to bacteria (a frequent phenomenon for recombinant proteins with hydropho ...
Negative Regulation of Rho Signaling by Insulin and Its
... of precleared lysate proteins (250 g) were incubated with 25 g (40 l) of the Rhotekin-Rho binding domain (RBD)-agarose slurry (UBI, Lake Placid, NY) and incubated for 1 h at 4°C with shaking. The agarose beads were washed with wash buffer according to the manufacturer’s protocol and resuspended i ...
... of precleared lysate proteins (250 g) were incubated with 25 g (40 l) of the Rhotekin-Rho binding domain (RBD)-agarose slurry (UBI, Lake Placid, NY) and incubated for 1 h at 4°C with shaking. The agarose beads were washed with wash buffer according to the manufacturer’s protocol and resuspended i ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.