Protein kinases - Institut de recherches cliniques de Montréal
... How many phosphorylation sites are there? If there are ~10,000 proteins per cell with an average length of 400 aa (~ 17% of which are Ser, Thr or Tyr), then there are ~700,000 potential phosphorylation sites for any given kinase (including hidden residues). Although protein kinases have relatively ...
... How many phosphorylation sites are there? If there are ~10,000 proteins per cell with an average length of 400 aa (~ 17% of which are Ser, Thr or Tyr), then there are ~700,000 potential phosphorylation sites for any given kinase (including hidden residues). Although protein kinases have relatively ...
Protein Stability Protein Folding
... substitutions (which affect the entropy of the denatured state, and thus its free energy) and increased number of salt bridges. • Each of these alone makes only a small effect, but several such changes are enough. In general, it appears that there is no single determinant of increased thermal stabil ...
... substitutions (which affect the entropy of the denatured state, and thus its free energy) and increased number of salt bridges. • Each of these alone makes only a small effect, but several such changes are enough. In general, it appears that there is no single determinant of increased thermal stabil ...
Lecture 1 - Edward Dennis - University of California San Diego
... Department of Pharmacology, School of Medicine University of California, San Diego Copyright/attribution notice: You are free to copy, distribute, adapt and transmit this tutorial or individual slides (without alteration) for academic, non-profit and non-commercial purposes. Attribution: Edward A. D ...
... Department of Pharmacology, School of Medicine University of California, San Diego Copyright/attribution notice: You are free to copy, distribute, adapt and transmit this tutorial or individual slides (without alteration) for academic, non-profit and non-commercial purposes. Attribution: Edward A. D ...
PACS-2 (Q-20): sc-160645 - Santa Cruz Biotechnology
... PACS-2 (phosphofurin acidic cluster sorting protein-2), also known as KIAA0602 or PACS1L, is an 889 amino acid protein that localizes to both the mitochondrion and the lumen of the endoplasmic reticulum (ER) and belongs to the PACS (phosphofurin acidic cluster sorting) family. Expressed in a broad r ...
... PACS-2 (phosphofurin acidic cluster sorting protein-2), also known as KIAA0602 or PACS1L, is an 889 amino acid protein that localizes to both the mitochondrion and the lumen of the endoplasmic reticulum (ER) and belongs to the PACS (phosphofurin acidic cluster sorting) family. Expressed in a broad r ...
Signal Perception and Transduction: The Origin of
... pathways used by plant cells have been clarified as a result of the enormous attention they have received over the last 7 years. Signals are first perceived by receptors. Virtually all known receptors are members of families of related proteins and are located in the plasma membrane, nucleus, cytopl ...
... pathways used by plant cells have been clarified as a result of the enormous attention they have received over the last 7 years. Signals are first perceived by receptors. Virtually all known receptors are members of families of related proteins and are located in the plasma membrane, nucleus, cytopl ...
PROTEINS OF SPERM NUCLEI EXAMINED BY
... of tracks of other nuclei. Such a distribution might be expected if 12 chromosomes were distributed at random to eight nuclei. The data from Tables 2 and 3 indicate at best, that a single chromosome should produce about seven or so electron tracks at the fourth or fifth division. The only conceivabl ...
... of tracks of other nuclei. Such a distribution might be expected if 12 chromosomes were distributed at random to eight nuclei. The data from Tables 2 and 3 indicate at best, that a single chromosome should produce about seven or so electron tracks at the fourth or fifth division. The only conceivabl ...
Proteins
... proteins in the body. Forms the amino acid pool: Exogenous amino acids from food Endogenous amino acids from within the body 2. Nitrogen Balance = Protein utilization in the body. Zero N balance in equilibrium. Positive N balance - N consumed is greater than excreted. Negative N balance - N excreted ...
... proteins in the body. Forms the amino acid pool: Exogenous amino acids from food Endogenous amino acids from within the body 2. Nitrogen Balance = Protein utilization in the body. Zero N balance in equilibrium. Positive N balance - N consumed is greater than excreted. Negative N balance - N excreted ...
PowerPoint 演示文稿
... A considerable proportion of LADCs, the most common histological type of lung cancer that comprises ~40% of the total cases, develops through activation of oncogenes, for example, somatic mutations in EGFR (10–50% of cases) or KRAS (10–30% of cases) or fusion of ALK (5% of cases), in a mutually e ...
... A considerable proportion of LADCs, the most common histological type of lung cancer that comprises ~40% of the total cases, develops through activation of oncogenes, for example, somatic mutations in EGFR (10–50% of cases) or KRAS (10–30% of cases) or fusion of ALK (5% of cases), in a mutually e ...
Anopheles gambiae Drosophila melanogaster Bo Lindberg
... Malaria is a disease of poverty and continues to plague a great part of the world’s population. An increased understanding of the interactions between the vector mosquito, the malaria parasite, and also the mosquito gut microbiota are pivotal for the development of novel measures against the disease ...
... Malaria is a disease of poverty and continues to plague a great part of the world’s population. An increased understanding of the interactions between the vector mosquito, the malaria parasite, and also the mosquito gut microbiota are pivotal for the development of novel measures against the disease ...
Amino Acid Starter Kit in Brief
... The cysteine amino acids can form disulfide bonds. Acidic and basic amino acids can form salt bridges, or electrostatic interactions. The hydrophobic sidechains are buried in the interior of a globular protein. The hydrophilic sidechains are usually exposed on the surface of a globular protein. The ...
... The cysteine amino acids can form disulfide bonds. Acidic and basic amino acids can form salt bridges, or electrostatic interactions. The hydrophobic sidechains are buried in the interior of a globular protein. The hydrophilic sidechains are usually exposed on the surface of a globular protein. The ...
Slide 1
... 1. Action potential reaches the axon terminal where the presynaptic element resides. 2. Causes the opening of CA+ channels. 3. Ca+ forces the movement of microtubules onto synaptic vesicles pressing them to the presynaptic element. 4. Vesicles bind to specific sites on the presynaptic element and op ...
... 1. Action potential reaches the axon terminal where the presynaptic element resides. 2. Causes the opening of CA+ channels. 3. Ca+ forces the movement of microtubules onto synaptic vesicles pressing them to the presynaptic element. 4. Vesicles bind to specific sites on the presynaptic element and op ...
Genetically Encoded Fluorescent Reporters of Histone
... proteins around which DNA is wrapped in chromatin. Phosphorylation, acetylation, and methylation of the N-terminal tails of histone proteins strongly influence transcription of neighboring genes by altering steric access to DNA and by introducing or removing protein docking sites.1 Despite the impor ...
... proteins around which DNA is wrapped in chromatin. Phosphorylation, acetylation, and methylation of the N-terminal tails of histone proteins strongly influence transcription of neighboring genes by altering steric access to DNA and by introducing or removing protein docking sites.1 Despite the impor ...
Catabolism
... Carbohydrates and other nutrients serve two functions in the metabolism of heterotrophic microorganisms: 1. They are oxidized to release energy 2. They supply carbon or building blocks for the synthesis of new cell constituents. • Amphibolic pathways: function both catabolically and anabolically ...
... Carbohydrates and other nutrients serve two functions in the metabolism of heterotrophic microorganisms: 1. They are oxidized to release energy 2. They supply carbon or building blocks for the synthesis of new cell constituents. • Amphibolic pathways: function both catabolically and anabolically ...
Awan, Ali: In Silico Transfer of Ligand Binding Function between Structurally Analogous Proteins
... A, go to 2. If, on the other hand, a functional motif for the binding of L to A is already known, search for it in A using any string search method. Once found, this region in A should be the subject of the procedure in 5 to 7. 2. Is the general structural location (and hence the locations in the a ...
... A, go to 2. If, on the other hand, a functional motif for the binding of L to A is already known, search for it in A using any string search method. Once found, this region in A should be the subject of the procedure in 5 to 7. 2. Is the general structural location (and hence the locations in the a ...
งานนำเสนอ PowerPoint
... • the first committed step is too slow to permit its substrate and product to equilibrate • most of other reactions in a pathway function close to equilibrium • committed step = rate-limiting step ...
... • the first committed step is too slow to permit its substrate and product to equilibrate • most of other reactions in a pathway function close to equilibrium • committed step = rate-limiting step ...
SOD binds cell-adhesive peroxidase - Journal of Cell Science
... surface; it could be released from the membrane with high salt. It was thus concluded that the peroxinectin-binding protein is an extracellular SOD (EC-SOD) and a peripheral membrane protein, presumably kept at the cell surface via ionic interaction with its C-terminal region. This interaction with ...
... surface; it could be released from the membrane with high salt. It was thus concluded that the peroxinectin-binding protein is an extracellular SOD (EC-SOD) and a peripheral membrane protein, presumably kept at the cell surface via ionic interaction with its C-terminal region. This interaction with ...
Review Convergent Extension - Massachusetts Institute of Technology
... Focusing on frogs and fish, we review the different cellular mechanisms and the well-conserved signaling pathways that underlie this process. One of the attractions of working on embryos is the fascination of watching them change their shape. Despite the temptation to study morphogenesis, most devel ...
... Focusing on frogs and fish, we review the different cellular mechanisms and the well-conserved signaling pathways that underlie this process. One of the attractions of working on embryos is the fascination of watching them change their shape. Despite the temptation to study morphogenesis, most devel ...
Deletion mutant of FGFR4 induces onion
... Fig. 4. Nuclear structures induced by ∆Ext/R4Tth are found in cells at various cell cycle stages. (A,B) COS-1 cells transfected with ∆Ext/R4Tth were incubated with BrdU for 18 hours, then fixed and labeled with antiBrdU (red) and anti-FGFR4 (green) antibodies. Yellow color indicates overlap between ...
... Fig. 4. Nuclear structures induced by ∆Ext/R4Tth are found in cells at various cell cycle stages. (A,B) COS-1 cells transfected with ∆Ext/R4Tth were incubated with BrdU for 18 hours, then fixed and labeled with antiBrdU (red) and anti-FGFR4 (green) antibodies. Yellow color indicates overlap between ...
Deletion mutant of FGFR4 induces onion
... Fig. 4. Nuclear structures induced by ∆Ext/R4Tth are found in cells at various cell cycle stages. (A,B) COS-1 cells transfected with ∆Ext/R4Tth were incubated with BrdU for 18 hours, then fixed and labeled with antiBrdU (red) and anti-FGFR4 (green) antibodies. Yellow color indicates overlap between ...
... Fig. 4. Nuclear structures induced by ∆Ext/R4Tth are found in cells at various cell cycle stages. (A,B) COS-1 cells transfected with ∆Ext/R4Tth were incubated with BrdU for 18 hours, then fixed and labeled with antiBrdU (red) and anti-FGFR4 (green) antibodies. Yellow color indicates overlap between ...
Technical White Paper SOMAmer® Reagent Specificity
... SOMAmer reagent specificity in the SOMAscan assay The highly multiplexed SOMAscan assay takes advantage of both of the specificity attributes of SOMAmer reagents described above (high affinity and slow dissociation rates for cognate proteins), and includes assay steps to maximize that specificity. F ...
... SOMAmer reagent specificity in the SOMAscan assay The highly multiplexed SOMAscan assay takes advantage of both of the specificity attributes of SOMAmer reagents described above (high affinity and slow dissociation rates for cognate proteins), and includes assay steps to maximize that specificity. F ...
Biophysics 101 Genomics and Computational Biology
... Immunoglobulin V region variants in hybridoma cells. I. Isolation of a variant with altered idiotypic and antigen binding specificity. In vitro selection for altered divalent metal specificity in the RNase P RNA. In vitro selection of zinc fingers with altered DNA-binding specificity. In vivo select ...
... Immunoglobulin V region variants in hybridoma cells. I. Isolation of a variant with altered idiotypic and antigen binding specificity. In vitro selection for altered divalent metal specificity in the RNase P RNA. In vitro selection of zinc fingers with altered DNA-binding specificity. In vivo select ...
Budding yeast Saccharomyces cerevisiae as a model to study
... It is clear from the works analyzed above and some others that the identification of protein carbonyl levels as a marker of oxidative damage of proteins has both advantages and disadvantages. Certainly, the techniques based on this principle are most commonly used because they are well developed, re ...
... It is clear from the works analyzed above and some others that the identification of protein carbonyl levels as a marker of oxidative damage of proteins has both advantages and disadvantages. Certainly, the techniques based on this principle are most commonly used because they are well developed, re ...
IGF signaling directs ventricular cardiomyocyte
... development, as mouse knockouts of ligand or receptor genes result in a hypoplastic phenotype (Lavine et al., 2005). Nonetheless, there are uncertainties related to their possible role as epicardial factors (see Discussion). We showed previously that an immortalized adult rat atrial epicardial cell ...
... development, as mouse knockouts of ligand or receptor genes result in a hypoplastic phenotype (Lavine et al., 2005). Nonetheless, there are uncertainties related to their possible role as epicardial factors (see Discussion). We showed previously that an immortalized adult rat atrial epicardial cell ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.