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Cholesterol Synthesis Regulation of cholesterol synthesis pathway
Cholesterol Synthesis Regulation of cholesterol synthesis pathway

... - affected individuals are slow to clear cholesterol from blood stream - passage of large gall stones is extremely painful ...
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The Basics of Cellular Respiration

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Jeopardy - Alfred State College intranet site

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... In brief, E. coli cells containing the gst-lcf expression plasmids were grown overnight at 23 °C in 2× YT-G medium (Pharmacia) supplemented with 100 µg/mL of ampicillin, then diluted 10-fold in fresh medium and grown for 3 h at 23 °C. Isopropyl β-D-thiogalactoside (IPTG) was added to a final concent ...
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22. Think of two different proteins: both are enzymes. a) What
22. Think of two different proteins: both are enzymes. a) What

... Phosphorylation/Dephosphorylation: requires donor of phosphate group (ATP=P1+energy) d) What is the main difference between the mechanism in c) and allosteric control? Two enzymes are needed to regulate for covalent binding in phosphorylation/de. and no enzymes are used for non covalent allosteric b ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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