Chem 109 C Fall 2014 Armen Zakarian Office: Chemistry Bldn 2217
... compositions are shown. Reaction of the intact decapeptide with Edman s reagent releases PTH-Gly. What is the sequence of the decapeptide? ...
... compositions are shown. Reaction of the intact decapeptide with Edman s reagent releases PTH-Gly. What is the sequence of the decapeptide? ...
Conversion of trypsin to a functional threonine protease
... hydroxyl group of Thr 195 to be used as a nucleophile in a manner similar to Ser 195, its hydroxyl group would have to be within hydrogen bonding distance the Ne2 of His 57 upon binding a peptide substrate. For efficient hydrogen bonding between Thr 195 and His 57 to occur, the methyl and hydroxyl g ...
... hydroxyl group of Thr 195 to be used as a nucleophile in a manner similar to Ser 195, its hydroxyl group would have to be within hydrogen bonding distance the Ne2 of His 57 upon binding a peptide substrate. For efficient hydrogen bonding between Thr 195 and His 57 to occur, the methyl and hydroxyl g ...
Biochemistry of Ensiling - DigitalCommons@University of Nebraska
... Two or more (up to 10) monosaccharides linked together are referred to as oligosaccharides (Kandler & Hopf, 1980). This is a bit of an arbitrary definition to draw a distinction between oligosaccharides and polysaccharides. In most cases naturally occurring oligosaccharides that are not intermediate ...
... Two or more (up to 10) monosaccharides linked together are referred to as oligosaccharides (Kandler & Hopf, 1980). This is a bit of an arbitrary definition to draw a distinction between oligosaccharides and polysaccharides. In most cases naturally occurring oligosaccharides that are not intermediate ...
Metabolism: Energy, Enzymes, and Regulation
... (e.g., a cell or a plant) called a system. All other matter in the universe is called the surroundings. Thermodynamics focuses on the energy differences between the initial state and the final state of a system. It is not concerned with the rate of the process. For instance, if a pan of water is hea ...
... (e.g., a cell or a plant) called a system. All other matter in the universe is called the surroundings. Thermodynamics focuses on the energy differences between the initial state and the final state of a system. It is not concerned with the rate of the process. For instance, if a pan of water is hea ...
Metabolic networks: enzyme function and metabolite structure
... example, 1.1 denotes oxidoreductases acting on the CH– OH group of donors. The third level of classification (k) denotes the cofactors used by the enzyme. For example, 1.1.1 denotes oxidoreductases acting on CH–OH groups using NADþ or NADPþ as acceptors. The fourth level (l) is specific to the subst ...
... example, 1.1 denotes oxidoreductases acting on the CH– OH group of donors. The third level of classification (k) denotes the cofactors used by the enzyme. For example, 1.1.1 denotes oxidoreductases acting on CH–OH groups using NADþ or NADPþ as acceptors. The fourth level (l) is specific to the subst ...
POULTRY BREEDING
... Mineral acids (hydrochloric, sulfuric, and phosphoric acids or the mixture of them), setting up the required pH; Organic acids: formic acid, propionic acid, Disadvantages of their use: - can disturb the mineral metabolism of the host, - danger in their use (corrosive, difficult to ...
... Mineral acids (hydrochloric, sulfuric, and phosphoric acids or the mixture of them), setting up the required pH; Organic acids: formic acid, propionic acid, Disadvantages of their use: - can disturb the mineral metabolism of the host, - danger in their use (corrosive, difficult to ...
Positively selected sites on the surface glycoprotein (G) of infectious
... also in a region of the molecule that does not change shape as the molecule converts from its prefusion state in the extracellular virion to the pH-activated state in the lysosome (Roche et al., 2007). This would allow for more amino acid substitutions in this region. Amino acid changes in a hinge r ...
... also in a region of the molecule that does not change shape as the molecule converts from its prefusion state in the extracellular virion to the pH-activated state in the lysosome (Roche et al., 2007). This would allow for more amino acid substitutions in this region. Amino acid changes in a hinge r ...
02/01/05 1 Cellulose-Degrading Symbioses BI 358 I. Intro: Guts of
... (short chain fatty acids), such as acetic and butyric acid and are then absorbed into the host body wall where they are substrates for aerobic respiration - fuel. a) Process analogous to production of EtOH by yeast 4. Symbionts can also contribute to nitrogen needs a) host diet often nitrogen-poor - ...
... (short chain fatty acids), such as acetic and butyric acid and are then absorbed into the host body wall where they are substrates for aerobic respiration - fuel. a) Process analogous to production of EtOH by yeast 4. Symbionts can also contribute to nitrogen needs a) host diet often nitrogen-poor - ...
Chem 352 - Fall 2014 - Exam II
... acid/base catalyst (as an acid) d. What catalytic mode does His 95 play in step 1 of this reaction? ___________________ acid/base catalyst (as a base) e. What catalytic role does Glu 165 play in step 1 of this reaction? ______________________ 4. Why are some sugars considered reducing sugars while o ...
... acid/base catalyst (as an acid) d. What catalytic mode does His 95 play in step 1 of this reaction? ___________________ acid/base catalyst (as a base) e. What catalytic role does Glu 165 play in step 1 of this reaction? ______________________ 4. Why are some sugars considered reducing sugars while o ...
Integration and Control - Academic Resources at Missouri Western
... – The juvenile stage for most conifers lasts 10 - 20 years. Exogenous application of GA can cause ...
... – The juvenile stage for most conifers lasts 10 - 20 years. Exogenous application of GA can cause ...
purification and properties of glutamate dehydrogenase from the
... activity ratios were 7 at pH 7.5 and 15 at pH 8. Enzyme kinetic constants (So.5) for NH~, ~-ketoglutarate and coenzyme were 210 _+ 10, 2.7 _+ 0.2 and 0.032 _+ 0.001 mM for the NADH linked reaction and 61 _+ 2.0, 0.56 _+ 0.06 and 0.060 _+ 0.003 mM for the NADPH reaction. Ammonium ion kinetics were di ...
... activity ratios were 7 at pH 7.5 and 15 at pH 8. Enzyme kinetic constants (So.5) for NH~, ~-ketoglutarate and coenzyme were 210 _+ 10, 2.7 _+ 0.2 and 0.032 _+ 0.001 mM for the NADH linked reaction and 61 _+ 2.0, 0.56 _+ 0.06 and 0.060 _+ 0.003 mM for the NADPH reaction. Ammonium ion kinetics were di ...
Glycolysis - Rose
... efficient, but much slower, oxidation of pyruvate. The fact that some microorganisms perform primarily anaerobic glycolysis under some conditions is also of benefit to humans: the production of ethanol and carbon dioxide from glucose is used for beer, wine, and bread production. In multicellular org ...
... efficient, but much slower, oxidation of pyruvate. The fact that some microorganisms perform primarily anaerobic glycolysis under some conditions is also of benefit to humans: the production of ethanol and carbon dioxide from glucose is used for beer, wine, and bread production. In multicellular org ...
... Restriction endonucleases can easily distinguish between these two sequences due to the formation of hydrogen bonds in the major groove. If restriction endonucleases bound in the minor groove would they be able to differentiate between an AT and a TA basepair? Begin by marking all non-watson crick h ...
ppt - Chair of Computational Biology
... disequilibrium (LD) with one another, and the numbers on the bottom indicate each of the 12 SNPs. SNP 9 is the causal variant, which in this simple example determines drug response: allele C results in a therapeutic response, whereas allele G results in an adverse reaction. In this example, the sele ...
... disequilibrium (LD) with one another, and the numbers on the bottom indicate each of the 12 SNPs. SNP 9 is the causal variant, which in this simple example determines drug response: allele C results in a therapeutic response, whereas allele G results in an adverse reaction. In this example, the sele ...
Sequential Expression of Macromolecule
... the intracellular effector controlling antibiotic synthesis is suggested by the work of Janglova, Suchy & Vanek (1969) and J. F. Martin, P. Liras & A. L. Demain (unpublished observations). Since oxygen uptake and DNA synthesis follow diphasic patterns in cultures synthesizing polyene macrolide antib ...
... the intracellular effector controlling antibiotic synthesis is suggested by the work of Janglova, Suchy & Vanek (1969) and J. F. Martin, P. Liras & A. L. Demain (unpublished observations). Since oxygen uptake and DNA synthesis follow diphasic patterns in cultures synthesizing polyene macrolide antib ...
The effect of pH on the digestion of proteins in vitro by pepsin
... but it has also been claimed (cf. Taylor, 1959a) that it shows a second peak of activity at pH 3’5-4 in the digestion of certain substrates, such as, for example, plasma proteins. Extracts of human and pig’s gastric mucosa frequently also show two peaks of activity one at pH 1.8-2 and the other at p ...
... but it has also been claimed (cf. Taylor, 1959a) that it shows a second peak of activity at pH 3’5-4 in the digestion of certain substrates, such as, for example, plasma proteins. Extracts of human and pig’s gastric mucosa frequently also show two peaks of activity one at pH 1.8-2 and the other at p ...
ICSB3: DRPM Measures
... (MIT/HMS), Ed DeLong (MIT BE), Chris Marx (Harvard OEB), Penny Chisholm (MIT Civil Eng). These basic enabling technologies feed into all of the other aims. We are improving our pipeline from 1. metagenomics (single cell sequencing) to 2. datamining to 3. combinatorial (semi)synthetic library formati ...
... (MIT/HMS), Ed DeLong (MIT BE), Chris Marx (Harvard OEB), Penny Chisholm (MIT Civil Eng). These basic enabling technologies feed into all of the other aims. We are improving our pipeline from 1. metagenomics (single cell sequencing) to 2. datamining to 3. combinatorial (semi)synthetic library formati ...
![[S], K m](http://s1.studyres.com/store/data/008275352_1-bf2876422b91ee7fa9eb4fe4480083af-300x300.png)
[S], K m
... stick and stickase. When the enzyme is complementary to the substrate, as in (b), the ES complex is more stable and has less free energy in the ground state than substrate alone. The result is an increase in the activation energy. For simplicity, the EP complexes are not shown. ...
... stick and stickase. When the enzyme is complementary to the substrate, as in (b), the ES complex is more stable and has less free energy in the ground state than substrate alone. The result is an increase in the activation energy. For simplicity, the EP complexes are not shown. ...
analyzing gene and protein sequences
... This disease, also known as PKU, is caused by an inborn error of metabolism. PKU is autosomal recessive, which means babies born with PKU have two abnormal copies of the gene phenylalanine hydroxylase. This gene codes for an enzyme that converts the amino acid phenylalanine to the amino acid tyrosin ...
... This disease, also known as PKU, is caused by an inborn error of metabolism. PKU is autosomal recessive, which means babies born with PKU have two abnormal copies of the gene phenylalanine hydroxylase. This gene codes for an enzyme that converts the amino acid phenylalanine to the amino acid tyrosin ...
Full text in pdf - International Microbiology
... bacterial strains. The enzyme responsible for this epimerization was first purified from actinomycetes [26,56], and the gene encoding its activity, cefD, was found to be located in the cephamycin gene cluster [6,23]. The bacterial cefD-encoded protein is a pyridoxal phosphate-dependent enzyme of abo ...
... bacterial strains. The enzyme responsible for this epimerization was first purified from actinomycetes [26,56], and the gene encoding its activity, cefD, was found to be located in the cephamycin gene cluster [6,23]. The bacterial cefD-encoded protein is a pyridoxal phosphate-dependent enzyme of abo ...
in Power-Point Format
... • E. coli trp operon contains 5 genes (E,D,C,B,A) for enzymes to make amino acid tryptophan (anabolic) • Anabolic enzyme synthesis typically on, and turned off by high level of product of pathway (repressed) • Operon subject to negative control of transcription by repressor when high tryptophan leve ...
... • E. coli trp operon contains 5 genes (E,D,C,B,A) for enzymes to make amino acid tryptophan (anabolic) • Anabolic enzyme synthesis typically on, and turned off by high level of product of pathway (repressed) • Operon subject to negative control of transcription by repressor when high tryptophan leve ...
Molecular modeling of HIV-1 reverse
... A more direct measure of this geometry alteration is the distance between the D186 residue (CA atom) near the dNTP binding site and a reference residue in the primer-grip region (Jacobo-Molina et al., 1993) of RT (G231:CA). Reduction of this distance to ,11.0 Å (wild-type 11.7 Å) appears to lead t ...
... A more direct measure of this geometry alteration is the distance between the D186 residue (CA atom) near the dNTP binding site and a reference residue in the primer-grip region (Jacobo-Molina et al., 1993) of RT (G231:CA). Reduction of this distance to ,11.0 Å (wild-type 11.7 Å) appears to lead t ...
Gene clusters for β-lactam antibiotics and control of their expression
... bacterial strains. The enzyme responsible for this epimerization was first purified from actinomycetes [26,56], and the gene encoding its activity, cefD, was found to be located in the cephamycin gene cluster [6,23]. The bacterial cefD-encoded protein is a pyridoxal phosphate-dependent enzyme of abo ...
... bacterial strains. The enzyme responsible for this epimerization was first purified from actinomycetes [26,56], and the gene encoding its activity, cefD, was found to be located in the cephamycin gene cluster [6,23]. The bacterial cefD-encoded protein is a pyridoxal phosphate-dependent enzyme of abo ...