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25. biosynthesis of lipids
25. biosynthesis of lipids

... This reaction is very similar to other biotin-dependent carboxylation reactions, such as those catalyzed by pyruvate carboxylase and propionyl-CoA carboxylase. Acetyl-CoA carboxylase is also important because it is a regulatory step ; citrate acts as an allosteric activator for the animal enzyme, bu ...
VITAMINS
VITAMINS

... of folic acids. Also involved in maturation of erythrocytes. ...
From DNA sequence to application: possibilities and
From DNA sequence to application: possibilities and

... various LAB species, have now become available (Table I). We are now in the position to compare their overall genetic organization and, by homology comparisons, assign probable functions to a number of their ORF's. Figure 1 displays a schematic representation of such a functional assignment for a sp ...
A four-column theory for the origin of the genetic code: tracing the
A four-column theory for the origin of the genetic code: tracing the

... code. Organisms that share the same code can also share transferred genes. Therefore there is an advantage to using common codes. It could be true that horizontal transfer was frequent at the time of the origin of the code and, if so, this could help to explain why a universal code spread through al ...
vitamine
vitamine

... Vitamins are divided up into two main groups which are fatsoluble vitamins and watersoluble vitamins. Fat-soluble vitamins are usually found in foods that contain fat. The body stores the fat soluble vitamins and because of this, people don’t usually need to make a special effort to include them in ...
20 Tricarboxylic Acid Cycle
20 Tricarboxylic Acid Cycle

... and aerobic exercise program of daily tennis and jogging (see Chapter 19). He has lost a total of 33 lb and is just 23 lb from his college weight of 154 lb. His exercise capacity has markedly improved; he can run for a longer time at a faster pace before noting shortness of breath or palpitations of ...
Disposition of Glutathione Conjugates in Rats by a Novel Glutamic
Disposition of Glutathione Conjugates in Rats by a Novel Glutamic

... mercapturic acid pathway is currently unknown. It is postulated that the ␥-carboxylic acid is activated (perhaps by ATP) before enzymatic addition to the ␣-amino group of cysteine or glutamate takes place. The discovery of these peptide conjugates of acetaminophen represents a novel disposition of g ...
KIEBER, ROBERT J., LINDA H. HYDRO, AND PAMELA J. SEATON
KIEBER, ROBERT J., LINDA H. HYDRO, AND PAMELA J. SEATON

Characterisation of the diol dehydratase pdu operon of Lactobacillus
Characterisation of the diol dehydratase pdu operon of Lactobacillus

... the degrees of homologies observed with other dehydratases, the molecular mass deduced from the ORFs as well as the genetic environment of these genes showed that this protein is rather a diol dehydratase than a glycerol dehydratase. Therefore, the name previously used to design the gene, dha [10], ...
Succinate Dehydrogenase of Saccharomyces cerevisiae
Succinate Dehydrogenase of Saccharomyces cerevisiae

... two spatially separated sites with an edge-to-edge distance of 25.4 Å. The new amino acid residues that may determine the structural or catalytic properties of each of the two quinone binding sites were identified. The model also provided insight into the unusual use of a cysteine (Sdh4p Cys78) as t ...
Comparative day/night metatranscriptomic analysis of microbial
Comparative day/night metatranscriptomic analysis of microbial

RED CELL MEMBRANE DEFECTS
RED CELL MEMBRANE DEFECTS

... enzymes involved in these pathways The availability of five RBC substrates: Glucose, Glutathione, NAD, NAD phosphate & Adenosine diphosphate (ADP) ...
7.6 Hydrolysis of Amides
7.6 Hydrolysis of Amides

... is as dangerous for our health as is the aspartame in diet soda! Nonetheless there have been (and still are) major objections about the widespread use of aspartame as an artificial sweetener. Note that the methanol that is produced is potentially toxic because it can be converted into formaldehyde b ...
Interactions between lactic and propionic acid bacteria
Interactions between lactic and propionic acid bacteria

... The free amino acid composition of control and RR wheys are shown in figure 3. Arginine and phenylalanine were not detected in either whey. Some amino acids (eg alanine, methionine, isoleucine, tyrosine, histidine and proline), which were not found in the control whey, were present in RR whey. In ad ...
Degradation signals within both terminal domains of the cauliflower
Degradation signals within both terminal domains of the cauliflower

... Introduction Protein degradation plays an important role in many cellular processes: it allows much faster alteration of the amount of regulatory proteins than transcriptional or translational regulation, and is important for the relocation of biochemical resources. Although protein degradation has ...
Phenyl Acetate Preparation ( from Phenol and
Phenyl Acetate Preparation ( from Phenol and

... Phenyl acetate preparation To a solution of 40 g phenol (0.425 mol) in 300 g glacial acetic acid (5.0 mol) was added 2 ml cone. sulfuricacid. This solutmn was boiled while slowly distilling at atmospheric pressure up an efficient column (100 X 3 cm) fitted with a variable reflux ratio head set at I ...
The acetyl-CoA pathway of autotrophic growth
The acetyl-CoA pathway of autotrophic growth

... the total synthesis of an organic compound from which the succeeding anabolic reactions proceed. ...
Carbon dioxide fixation.
Carbon dioxide fixation.

regulation of mammalian acetyl
regulation of mammalian acetyl

... by cAMP-dependent protein kinase and ACC kinase (67) causes primarily an increase in the Ka for citrate and a more modest decrease in Vmax (67). The isolation and characterization of the various phosphopeptides of ACC-α that can be phosphorylated by different protein kinases in vitro and in vivo (32 ...
Document
Document

... KEY REACTIONS of GLYCOLYSIS substrate level phosphorylation redox reaction involving NAD ...
How did LUCA make a living?
How did LUCA make a living?

... of CO2 to acetyl CoA without the participation of ATP or any other triphosphate. Transition metal sulphides abound in the methanogen version of the acetyl CoA pathway,(6) but the universal energy currency ATP is missing. Instead, thioesters like acetyl CoA are central to the bioenergetics of the mos ...
Ser Trp Thr His Glu Asn Gly Lys His Val Trp Arg
Ser Trp Thr His Glu Asn Gly Lys His Val Trp Arg

... Biomolecular folding is a type of molecular self-assembly in which self-interaction drives a molecule to its native conformation. We synthesized six 12-amino acid β-hairpin peptides stabilized by π-π interactions between the indole groups of two tryptophan residues. Histidine binding pockets of vary ...
Arabidopsis Contains Nine Long-Chain Acyl
Arabidopsis Contains Nine Long-Chain Acyl

... characterize genes that encode LACSs in Arabidopsis. Nine cDNAs were identified, cloned, and tested for their ability to complement a LACS-deficient strain of yeast (Saccharomyces cerevisiae). Seven of the nine successfully restored growth, whereas two cDNAs encoding putative peroxisomal isoforms di ...
Substrate orientation and the origin of catalytic power in xanthine
Substrate orientation and the origin of catalytic power in xanthine

... the reaction (in this case, through to formation of the LMoIVO(SH)(OR) intermediate) decreased by two orders of magnitude from 1.97 × 106 M-1s-1 to 1.34 × 104 M-1s-1. At face value, the effect on kred and Kd is such that the interaction between Glu 232 and substrate provides some 3 kcal/mol of free ...
Stepwise Acquisition of Pyrimethamine Resistance in the Malaria
Stepwise Acquisition of Pyrimethamine Resistance in the Malaria

... Fig. 1. A flow chart of the computer algorithm is provided in Fig. S1. Briefly, replicate evolutionary landscapes were defined by random sampling of IC50 values for each of the 16 possible alleles from normal distributions with the allele-specific genotypic means and standard deviations depicted in ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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