The role of cytosolic proteins in the insertion of tail
... carrier cytochrome b5 (b5) and the protein tyrosine phosphatase (PTP)-1B, are instead able to translocate at nanomolar ATP concentrations into pure lipid vesicles, provided that these have low levels of cholesterol (Brambillasca et al., 2006; Brambillasca et al., 2005). We have called this latter me ...
... carrier cytochrome b5 (b5) and the protein tyrosine phosphatase (PTP)-1B, are instead able to translocate at nanomolar ATP concentrations into pure lipid vesicles, provided that these have low levels of cholesterol (Brambillasca et al., 2006; Brambillasca et al., 2005). We have called this latter me ...
A Simple 1-D Physical Model for the Crawling Nematode Sperm Cell
... interaction is discussed in more detail in ref. 10. In the model, we assume that adhesion between the cytoskeleton and the membrane is a function of pH, such that lowering the pH disrupts adhesion. (8) Two factors regulate the rate of MSP assembly at the front. First, this rate is controlled by pH: ...
... interaction is discussed in more detail in ref. 10. In the model, we assume that adhesion between the cytoskeleton and the membrane is a function of pH, such that lowering the pH disrupts adhesion. (8) Two factors regulate the rate of MSP assembly at the front. First, this rate is controlled by pH: ...
The effects of 405 nm light on bacterial membrane integrity
... stained cells with SYTOX green (Life Technologies), a high-affinity nucleic acid stain that can only permeate cells with compromised plasma membranes. For this technique, light-exposed samples, at a density of 109 c.f.u. ml 1, were centrifuged (as previously described) and cell pellets were immediat ...
... stained cells with SYTOX green (Life Technologies), a high-affinity nucleic acid stain that can only permeate cells with compromised plasma membranes. For this technique, light-exposed samples, at a density of 109 c.f.u. ml 1, were centrifuged (as previously described) and cell pellets were immediat ...
p53 and Apoptosis - Website Staff UI
... • Mutated/inactivated in a majority of human cancers • p53 -/- knockout mice embryos develop normally to term but after a few weeks the young mice develop numerous malignant tumors • adenovirus introduced wild-type p53 markedly enhanced the antitumor effect of a common chemotherapeutic agent, cispla ...
... • Mutated/inactivated in a majority of human cancers • p53 -/- knockout mice embryos develop normally to term but after a few weeks the young mice develop numerous malignant tumors • adenovirus introduced wild-type p53 markedly enhanced the antitumor effect of a common chemotherapeutic agent, cispla ...
Parts of the Cell In
... the cell there are many organelles, or little organs. These carry out many of the life’s functions like your organs. Please move on to the next slide and click on the boxes to see the names and functions of the cells organelles. ...
... the cell there are many organelles, or little organs. These carry out many of the life’s functions like your organs. Please move on to the next slide and click on the boxes to see the names and functions of the cells organelles. ...
Growth Control: A Saga of Cell Walls, ROS, and
... pollen tube is induced by ROS application and is required for ROS-induced bursting. It is intriguing in this context that rbohdeficient mutant pollen tubes (see below; Boisson-Dernier et al., 2013; Lassig et al., 2014) and root hairs (Duan et al., 2010) with low internal levels of ROS also display lo ...
... pollen tube is induced by ROS application and is required for ROS-induced bursting. It is intriguing in this context that rbohdeficient mutant pollen tubes (see below; Boisson-Dernier et al., 2013; Lassig et al., 2014) and root hairs (Duan et al., 2010) with low internal levels of ROS also display lo ...
Polarization of Endocytosis and Receptor
... protein, HRP occurs by fluid pinocytosis : that is, HRP does not bind to surface receptors but enters the cells during their normal uptake of medium (40, 41) . The uptake of HRP in J774 .2 cells follows these same kinetics (data not shown) . Furthermore, in J774.2 cells fluorescein- and rhodamine-co ...
... protein, HRP occurs by fluid pinocytosis : that is, HRP does not bind to surface receptors but enters the cells during their normal uptake of medium (40, 41) . The uptake of HRP in J774 .2 cells follows these same kinetics (data not shown) . Furthermore, in J774.2 cells fluorescein- and rhodamine-co ...
BI0I 121 cell and tissues
... State the different phases of the eu]caryotic cell cycle and tell what happens during each phase; contrast division of the eukaryotic to that of the prokaryotic cell; compare cyokinesis of plant and animal cells. Describe the different phases of mitosis; relate mitosis to DNA duplication; make carto ...
... State the different phases of the eu]caryotic cell cycle and tell what happens during each phase; contrast division of the eukaryotic to that of the prokaryotic cell; compare cyokinesis of plant and animal cells. Describe the different phases of mitosis; relate mitosis to DNA duplication; make carto ...
Programmed Changes in Form during Moss Development
... showed that the rise in membrane-associated calcium was not due to an increase in membrane density. The authors concluded that the increases in membrane-associated calcium reflected a localized increase in intracellular free calcium concentration. Further evidence that calcium plays an important rol ...
... showed that the rise in membrane-associated calcium was not due to an increase in membrane density. The authors concluded that the increases in membrane-associated calcium reflected a localized increase in intracellular free calcium concentration. Further evidence that calcium plays an important rol ...
Digging for the roots of amoeboid motility
... Even deeper insight can be obtained from phylogenetic studies of cell biological processes: It has become clear that suites of proteins involved in a particular structure or function are often maintained or lost in tandem. These observations can define the proteins involved in particular cellular fu ...
... Even deeper insight can be obtained from phylogenetic studies of cell biological processes: It has become clear that suites of proteins involved in a particular structure or function are often maintained or lost in tandem. These observations can define the proteins involved in particular cellular fu ...
Identification of a New Protein Localized at Sites of Cell
... a modification of the method of Cox, Schenk, and Olmsted (10). The 82-kD protein was partially purified from a low ionic strength extract of chicken gizzard smooth muscle followed by ammonium sulfate fractionation and ion-exchange chromatography. The column fractions were assayed for the presence of ...
... a modification of the method of Cox, Schenk, and Olmsted (10). The 82-kD protein was partially purified from a low ionic strength extract of chicken gizzard smooth muscle followed by ammonium sulfate fractionation and ion-exchange chromatography. The column fractions were assayed for the presence of ...
Structural Medicine II - European Science Foundation
... within a single cell and that many of these lipids are involved in modulating the processes of life in an area that is upcoming, lipidomics describes and quantitatively analyses the full complement of lipids, in the human body for example body fluids, cells and tissues. Lipidomics integrates these d ...
... within a single cell and that many of these lipids are involved in modulating the processes of life in an area that is upcoming, lipidomics describes and quantitatively analyses the full complement of lipids, in the human body for example body fluids, cells and tissues. Lipidomics integrates these d ...
Protozoa
... Cytoplasm is the living substance in which all the chemical reactions necessary for life are carried out. Ectoplasm is a clear gel-like layer enclosing the endoplasm which is more fluid and contains granules and other inclusions. Nucleus controls most of the reactions taking place in the cell and pl ...
... Cytoplasm is the living substance in which all the chemical reactions necessary for life are carried out. Ectoplasm is a clear gel-like layer enclosing the endoplasm which is more fluid and contains granules and other inclusions. Nucleus controls most of the reactions taking place in the cell and pl ...
The Eye and Ear (model-based undergrad
... on the models, identify them on the ear of yourself, another student, or a TA. The middle ear is separated from the external ear by the tympanic membrane and consists of an air-filled tympanic cavity. Identify the tympanic membrane and tympanic cavity on the models. 1) What are the names of the thre ...
... on the models, identify them on the ear of yourself, another student, or a TA. The middle ear is separated from the external ear by the tympanic membrane and consists of an air-filled tympanic cavity. Identify the tympanic membrane and tympanic cavity on the models. 1) What are the names of the thre ...
The Polymeric Immunoglobulin Receptor Accumulates in
... PCI2 cells stably transfected with plgR were differentiated in the presence of NGF on matrigel-coated chamber slides as described above. For some experiments, cells stably expressing pIgR were transiently transfected with eDNA encoding the human LDL-R before differentiation. Control experiments were ...
... PCI2 cells stably transfected with plgR were differentiated in the presence of NGF on matrigel-coated chamber slides as described above. For some experiments, cells stably expressing pIgR were transiently transfected with eDNA encoding the human LDL-R before differentiation. Control experiments were ...
Chapter 9 - Fullfrontalanatomy.com
... not be able to express your wishes to others, nor could you ask questions to find out more about your environment. Social organization is dependent on communication between the individuals that comprise that society; without communication, society would fall apart. As with people, it is vital for in ...
... not be able to express your wishes to others, nor could you ask questions to find out more about your environment. Social organization is dependent on communication between the individuals that comprise that society; without communication, society would fall apart. As with people, it is vital for in ...
Diverse Effects of Mutations in the Signal Sequence on the Secretion of b-lactamase in Salmonella typhimurium.
... the precursors synthesized by the remaining mutants resemble wild-type in that they remain trypsin-inaccessible. One of the latter mutants does produce mature protein, but at a very reduced rate. It thus appears that signalsequence mutations can affect more than one step in the secretion process, an ...
... the precursors synthesized by the remaining mutants resemble wild-type in that they remain trypsin-inaccessible. One of the latter mutants does produce mature protein, but at a very reduced rate. It thus appears that signalsequence mutations can affect more than one step in the secretion process, an ...
PDF
... 2008; Olmedo-Verd et al., 2011). Membrane anchoring was particularly well demonstrated for the Valyl-tRNA synthetase (ValRS) of Anabaena sp. PCC 7120 (hereafter Anabaena), which harbors an idiosyncratic domain, termed CAAD, with a putative C-terminal coiled-coil and two transmembrane helices, that m ...
... 2008; Olmedo-Verd et al., 2011). Membrane anchoring was particularly well demonstrated for the Valyl-tRNA synthetase (ValRS) of Anabaena sp. PCC 7120 (hereafter Anabaena), which harbors an idiosyncratic domain, termed CAAD, with a putative C-terminal coiled-coil and two transmembrane helices, that m ...
Organelle Fission. Crossing the Evolutionary Divide
... this conclusion is still tentative, as there are only three full-length FtsZ2 genes from higher plants currently represented in the public databases. Nevertheless, the predicted localizations of FtsZ1 and FsZ2 correspond with electron micrographs of dividing chloroplasts in which dense deposits term ...
... this conclusion is still tentative, as there are only three full-length FtsZ2 genes from higher plants currently represented in the public databases. Nevertheless, the predicted localizations of FtsZ1 and FsZ2 correspond with electron micrographs of dividing chloroplasts in which dense deposits term ...
Targeting of P-Selectin to Two Regulated Secretory Organelles in
... monocytes and neutrophils following its regulated appearance on the cell surface as part of the inflammatory response (24, 30). Expression in the pituicyte line AtT-20 revealed that it was targeted to the secretory granules and studies using chimeras revealed that the cytoplasmic domain was both nec ...
... monocytes and neutrophils following its regulated appearance on the cell surface as part of the inflammatory response (24, 30). Expression in the pituicyte line AtT-20 revealed that it was targeted to the secretory granules and studies using chimeras revealed that the cytoplasmic domain was both nec ...
Section 3A Analysis on a Western Blot
... In some Western blots, especially under nonoptimal conditions, the detecting antibody may bind non-specifically to non-tagged proteins in the sample. To avoid confusion in interpreting the band pattern, always include proper controls on the blot. These controls should not contain the tagged protein, ...
... In some Western blots, especially under nonoptimal conditions, the detecting antibody may bind non-specifically to non-tagged proteins in the sample. To avoid confusion in interpreting the band pattern, always include proper controls on the blot. These controls should not contain the tagged protein, ...
Localization of Light-harvesting Complex II to the Occluded Surfaces
... there is no evidencel for extrinsic proteins associated with the outer stacked surface. In contrast, the large particle on the outer nonstacked surface is the extrinsic subunit of the ATP synthase, CF~ (Miller and Staehelin, 1976) with a molecular weight in excess of 350,000. The tetrameric particle ...
... there is no evidencel for extrinsic proteins associated with the outer stacked surface. In contrast, the large particle on the outer nonstacked surface is the extrinsic subunit of the ATP synthase, CF~ (Miller and Staehelin, 1976) with a molecular weight in excess of 350,000. The tetrameric particle ...
Cell shape determination in Escherichia coli
... hence a change in the stress placed on the cell envelope. The stress theory of morphogenesis is discussed in detail by Harold in this issue. It should be emphasized, however, that there are other possible transduction mechanisms, for example, changes in concentrations of second messengers or other s ...
... hence a change in the stress placed on the cell envelope. The stress theory of morphogenesis is discussed in detail by Harold in this issue. It should be emphasized, however, that there are other possible transduction mechanisms, for example, changes in concentrations of second messengers or other s ...
Pulsatile Stretch Remodels Cell-to-Cell Communication in Cultured
... cultured for 4 days as confluent monolayers on thin silicone membranes and then subjected to linear pulsatile stretch for up to 6 hours. Action potential upstrokes and propagation velocity (⌰) were measured with multisite optical recording of transmembrane voltage of the cells stained with the volta ...
... cultured for 4 days as confluent monolayers on thin silicone membranes and then subjected to linear pulsatile stretch for up to 6 hours. Action potential upstrokes and propagation velocity (⌰) were measured with multisite optical recording of transmembrane voltage of the cells stained with the volta ...
Cell membrane
The cell membrane (also known as the plasma membrane or cytoplasmic membrane) is a biological membrane that separates the interior of all cells from the outside environment. The cell membrane is selectively permeable to ions and organic molecules and controls the movement of substances in and out of cells. The basic function of the cell membrane is to protect the cell from its surroundings. It consists of the phospholipid bilayer with embedded proteins. Cell membranes are involved in a variety of cellular processes such as cell adhesion, ion conductivity and cell signalling and serve as the attachment surface for several extracellular structures, including the cell wall, glycocalyx, and intracellular cytoskeleton. Cell membranes can be artificially reassembled.