Novel Expression Pattern of Cytosolic Gln
... bands representing GS subunits. The mass of the major polypeptide band (43 kD) was slightly larger than the predicted monomer from the translated amino acid sequence of DgGS1-1 (39 kD). This major nodule polypeptide band corresponded to the single band observed in the root extracts (Fig. 4) and fall ...
... bands representing GS subunits. The mass of the major polypeptide band (43 kD) was slightly larger than the predicted monomer from the translated amino acid sequence of DgGS1-1 (39 kD). This major nodule polypeptide band corresponded to the single band observed in the root extracts (Fig. 4) and fall ...
13835_Amplite™ Fluorimetric Ascorbic Acid Assay
... L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense. Ascorbate is produced from glucose in the liver of most mammalian species. For humans ascorbate has to be obtained from food to survive, and a lack of sufficient Vitamin ...
... L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense. Ascorbate is produced from glucose in the liver of most mammalian species. For humans ascorbate has to be obtained from food to survive, and a lack of sufficient Vitamin ...
A New Species of Actinomycete, Amycolata alni
... Cultural properties. The cultural characteristics of Amycolata autotrophica and Amycolata alni strains were similar, corresponding to those given in the description of Amycolata autotrophica (19). All of the strains produced aerial mycelia which were white to cream colored. However, the color of the ...
... Cultural properties. The cultural characteristics of Amycolata autotrophica and Amycolata alni strains were similar, corresponding to those given in the description of Amycolata autotrophica (19). All of the strains produced aerial mycelia which were white to cream colored. However, the color of the ...
Nutrition, Metabolism, and Body Temperature Regulation
... Chapter 24 Nutrition, Metabolism, and Body Temperature Regulation ...
... Chapter 24 Nutrition, Metabolism, and Body Temperature Regulation ...
(EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts
... A cDNA encoding the Calvin cycle enzyme transketolase (TKL; EC 2.2.1.1) was isolated from Sorghum bicolor via subtractive differential hybridization, and used to isolate several full-length cDNA clones for this enzyme from spinach. Functional identity of the encoded mature subunit was shown by an 8. ...
... A cDNA encoding the Calvin cycle enzyme transketolase (TKL; EC 2.2.1.1) was isolated from Sorghum bicolor via subtractive differential hybridization, and used to isolate several full-length cDNA clones for this enzyme from spinach. Functional identity of the encoded mature subunit was shown by an 8. ...
Tissue-specific expression of AUX1 in maize roots
... auxin distribution in the maize primary root is still unclear. This is because the pattern of ZmAUX1 in situ signals (Fig. 3) and the distribution of auxin (see Fig. 3 in Kerk and Feldman 1995) do not completely overlap. Besides the root system, ZmAUX1 expression was also observed in mesocotyls and ...
... auxin distribution in the maize primary root is still unclear. This is because the pattern of ZmAUX1 in situ signals (Fig. 3) and the distribution of auxin (see Fig. 3 in Kerk and Feldman 1995) do not completely overlap. Besides the root system, ZmAUX1 expression was also observed in mesocotyls and ...
Key Residues Controlling Binding of Diverse Ligands to Human
... The ligand that bound the tightest to both enzymes is 8-methoxypsoralen. In contrast, nicotine had the lowest affinity for both enzymes. MAP, PEITC, and coumarin had intermediate KD values. In terms of differential affinities, the compounds fall into three groups. First, coumarin binds equally well ...
... The ligand that bound the tightest to both enzymes is 8-methoxypsoralen. In contrast, nicotine had the lowest affinity for both enzymes. MAP, PEITC, and coumarin had intermediate KD values. In terms of differential affinities, the compounds fall into three groups. First, coumarin binds equally well ...
Mutations in type I collagen genes resulting in osteogenesis
... gene in which the mutation did occur. Mutations in the COL1A1 gene may be highly deleterious, and even lethal, because they comprise three-quarters of all the procollagen molecules of type I synthesized (Fig. 3). In contrast, similar mutations in the COL1A2 gene would result in the loss of only half ...
... gene in which the mutation did occur. Mutations in the COL1A1 gene may be highly deleterious, and even lethal, because they comprise three-quarters of all the procollagen molecules of type I synthesized (Fig. 3). In contrast, similar mutations in the COL1A2 gene would result in the loss of only half ...
characterization of proteins from the cytoskeleton of giardia lamblia
... Proteins from the axonemes and disc cytoskeleton of Giardia lamblia have been examined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. In addition to tubulin and the 30 X 103 molecular weight disc protein, at least 18 minor components copurify with the two major proteins in Triton-ins ...
... Proteins from the axonemes and disc cytoskeleton of Giardia lamblia have been examined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. In addition to tubulin and the 30 X 103 molecular weight disc protein, at least 18 minor components copurify with the two major proteins in Triton-ins ...
Identification of genomic features in the classification of loss
... Methods: In order to collect experimentally verified LoF and GoF mutational information, we obtained 816 LoF mutations and 474 GoF mutations from a literature text-mining process. Next, with data-preprocessing steps, 258 LoF and 129 GoF mutations remained for a further analysis. We analyzed the prop ...
... Methods: In order to collect experimentally verified LoF and GoF mutational information, we obtained 816 LoF mutations and 474 GoF mutations from a literature text-mining process. Next, with data-preprocessing steps, 258 LoF and 129 GoF mutations remained for a further analysis. We analyzed the prop ...
A comparison of scoring functions for protein sequence
... function varies but other design elements are maintained, allowing us to isolate any differences that are due to the position scoring function alone. 1.1 Profile-profile alignment algorithms Most profile-profile methods can be viewed as variants of the well-known algorithms for pairwise sequence ali ...
... function varies but other design elements are maintained, allowing us to isolate any differences that are due to the position scoring function alone. 1.1 Profile-profile alignment algorithms Most profile-profile methods can be viewed as variants of the well-known algorithms for pairwise sequence ali ...
Bile Acids and Bile Acid Sequestrants
... The small heterodimer partner (SHP) protein is a member of the nuclear receptor family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore tec ...
... The small heterodimer partner (SHP) protein is a member of the nuclear receptor family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore tec ...
Bio CET 2015 Key Answers
... Hibernating animals have tissues containing mitochondria with a membrane protein that accelerates electron transport while blocking the synthesis of ATP. What is the consequence of this ? 1) Hibernating animals can synthesize fat instead of wasting energy of respiration. 2) Energy is saved because g ...
... Hibernating animals have tissues containing mitochondria with a membrane protein that accelerates electron transport while blocking the synthesis of ATP. What is the consequence of this ? 1) Hibernating animals can synthesize fat instead of wasting energy of respiration. 2) Energy is saved because g ...
Complementary DNA Cloning, Messenger RNA
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
Nucleotide sequence and taxonomic value of the
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
PDH02 - OSU Biochemistry and Molecular Biology
... from octanoic acid via an enzymatic S-insertion. Additional details have been learned about lipoic acid synthesis. The question of why mitochondria synthesize fatty acids has been answered: the synthesis of lipoic acid. Wada, Shintani, and Ohlrogge (17) established that pea mitochondria can acyl car ...
... from octanoic acid via an enzymatic S-insertion. Additional details have been learned about lipoic acid synthesis. The question of why mitochondria synthesize fatty acids has been answered: the synthesis of lipoic acid. Wada, Shintani, and Ohlrogge (17) established that pea mitochondria can acyl car ...
Novel Allergen Structures with Tandem Amino Acid Repeats
... Sequence Analysis of Bla g 1 Clones, Homology with Mosquito ANG12 Precursor—Initially, we compared the sequence similarity of the three Bla g 1 clones with the antigenically related allergen from P. americana, Per a 1 (16). The results showed 70 –72% sequence identity between Per a 1, Bla g 1.01, an ...
... Sequence Analysis of Bla g 1 Clones, Homology with Mosquito ANG12 Precursor—Initially, we compared the sequence similarity of the three Bla g 1 clones with the antigenically related allergen from P. americana, Per a 1 (16). The results showed 70 –72% sequence identity between Per a 1, Bla g 1.01, an ...
Cloning and Characterization of Unusual Fatty Acid Desaturases
... Rothamsted Research, Harpenden, Herts AL5 2JQ, United Kingdom The seed oil of Anemone leveillei contains significant amounts of sciadonic acid (20:3D5,11,14; SA), an unusual non-methyleneinterrupted fatty acid with pharmaceutical potential similar to arachidonic acid. Two candidate cDNAs (AL10 and A ...
... Rothamsted Research, Harpenden, Herts AL5 2JQ, United Kingdom The seed oil of Anemone leveillei contains significant amounts of sciadonic acid (20:3D5,11,14; SA), an unusual non-methyleneinterrupted fatty acid with pharmaceutical potential similar to arachidonic acid. Two candidate cDNAs (AL10 and A ...
Supporting document 1 Safety assessment
... properties of AHAS expressed in CV127. These studies demonstrated that the AHAS protein expressed in CV127 is as expected in terms of its physicochemical and functional properties but the mature form of protein is slightly larger (by 34 amino acids) than anticipated due to the N-terminal signal pept ...
... properties of AHAS expressed in CV127. These studies demonstrated that the AHAS protein expressed in CV127 is as expected in terms of its physicochemical and functional properties but the mature form of protein is slightly larger (by 34 amino acids) than anticipated due to the N-terminal signal pept ...
Metabolic profiling indicates impaired pyruvate dehydrogenase
... pathway or from breakdown of certain amino acids. Under normal aerobic conditions, pyruvate is transported into mitochondria, where it is converted to acetyl-CoA by the pyruvate dehydrogenase (PDH) complex. In addition, acetyl-CoA is produced independently of PDH, via degradation of fatty acids and ...
... pathway or from breakdown of certain amino acids. Under normal aerobic conditions, pyruvate is transported into mitochondria, where it is converted to acetyl-CoA by the pyruvate dehydrogenase (PDH) complex. In addition, acetyl-CoA is produced independently of PDH, via degradation of fatty acids and ...
Sources of blood glucose
... Hormonal Control of Blood Glucose • Endocrine pancreas – Only 2% of entire pancreas, – the rest produces digestive enzymes ...
... Hormonal Control of Blood Glucose • Endocrine pancreas – Only 2% of entire pancreas, – the rest produces digestive enzymes ...
1 - Plant Research International
... The method of entry into pathway 2 will be by pulse labelling of the roots of rooted cloves in nutrient medium containing 35SO4 followed by analysis of the pattern of labelling in the leaves. Particular attention will be paid to the labelling in the S-allyl--glutamyl cysteine and S-allyl cysteine. ...
... The method of entry into pathway 2 will be by pulse labelling of the roots of rooted cloves in nutrient medium containing 35SO4 followed by analysis of the pattern of labelling in the leaves. Particular attention will be paid to the labelling in the S-allyl--glutamyl cysteine and S-allyl cysteine. ...
Quick Guide - Fulvic Acid
... supercharged antioxidants, nutrients, free-radical scavengers, super oxide dismutases, enzymes, hormones, amino acids, antibiotics, fungicides and antivirals. Fulvic Acid has proved to be one of nature's most powerful organic electrolytes and it serves to balance cell life. When powerful organic ele ...
... supercharged antioxidants, nutrients, free-radical scavengers, super oxide dismutases, enzymes, hormones, amino acids, antibiotics, fungicides and antivirals. Fulvic Acid has proved to be one of nature's most powerful organic electrolytes and it serves to balance cell life. When powerful organic ele ...
Regulation of Stage I1 of Sporulation in Bacillus subtilis
... that, at t4, 3% of the cells have reached stage I1 and 6% have reached a malformed stage 111, viz. the engulfed ‘prespore’ appears to be surrounded by a single instead of a double membrane; the remaining cells are blocked at stage 0. In both mutants, about 1 in 10000 cells makes a heatresistant spor ...
... that, at t4, 3% of the cells have reached stage I1 and 6% have reached a malformed stage 111, viz. the engulfed ‘prespore’ appears to be surrounded by a single instead of a double membrane; the remaining cells are blocked at stage 0. In both mutants, about 1 in 10000 cells makes a heatresistant spor ...
Regulation of Stage I1 of Sporulation in Bacillus subtilis
... that, at t4, 3% of the cells have reached stage I1 and 6% have reached a malformed stage 111, viz. the engulfed ‘prespore’ appears to be surrounded by a single instead of a double membrane; the remaining cells are blocked at stage 0. In both mutants, about 1 in 10000 cells makes a heatresistant spor ...
... that, at t4, 3% of the cells have reached stage I1 and 6% have reached a malformed stage 111, viz. the engulfed ‘prespore’ appears to be surrounded by a single instead of a double membrane; the remaining cells are blocked at stage 0. In both mutants, about 1 in 10000 cells makes a heatresistant spor ...
Genetic code
The genetic code is the set of rules by which information encoded within genetic material (DNA or mRNA sequences) is translated into proteins by living cells. Biological decoding is accomplished by the ribosome, which links amino acids in an order specified by mRNA, using transfer RNA (tRNA) molecules to carry amino acids and to read the mRNA three nucleotides at a time. The genetic code is highly similar among all organisms and can be expressed in a simple table with 64 entries.The code defines how sequences of these nucleotide triplets, called codons, specify which amino acid will be added next during protein synthesis. With some exceptions, a three-nucleotide codon in a nucleic acid sequence specifies a single amino acid. Because the vast majority of genes are encoded with exactly the same code (see the RNA codon table), this particular code is often referred to as the canonical or standard genetic code, or simply the genetic code, though in fact some variant codes have evolved. For example, protein synthesis in human mitochondria relies on a genetic code that differs from the standard genetic code.While the genetic code determines the protein sequence for a given coding region, other genomic regions can influence when and where these proteins are produced.