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Supplementary Information (doc 82K)
Supplementary Information (doc 82K)

... Supplemental Figure S9. Sequence alignment of the Leishmania ATP-dependent DEAD-box helicase HEL67 with homologues from other eukaryotes. Sequence comparison of L. infantum HEL67 with corresponding homologues using ClustalW2. The ClustalW2 aligned sequences were used for box shade by BOXSHADE 3.21 p ...
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is

... designed to encode FDH (Fig. 3). Several clones were thus identified. The cloned cDNA encoded 377 amino acids of an open reading frame corresponding to a protein with a molecular mass of 41.5 kD and a pI of 6.7. The deduced molecular mass and pI were those of W in 2D PAGE. A sequence consisting of 2 ...
Fatty Acid Synthesis
Fatty Acid Synthesis

... entering Krebs Cycle. ...
Farnesyl diphosphate synthase, the target for nitrogen
Farnesyl diphosphate synthase, the target for nitrogen

... the mammalian mevalonate pathway enzymes remains unclear. The pathway has also been investigated in plants and it appears that some, but not all, of the enzymes are peroxisomal, and that there may also be mitochondrial isoenzymes of both isopentenyl diphosphate isomerase and FDPS [3,11–13]. However, ...
Document
Document

... Two consecutive phenylalanine residues are present in the flap of Bla g 2. The sequence alignment of Bla g 2 with pepsin and chymosin, performed for molecular modeling, showed that the second F coincided with Y75 in active aspartic proteinases, an important residue for catalytic activity.8 Therefore ...
Sequence-based predictions of membrane-protein topology, homology and insertion
Sequence-based predictions of membrane-protein topology, homology and insertion

... Life is a complex thing (Keyes 1966). During the short time a biological cell spends on earth before dividing itself into two new cells, or worse, committing suicide (Hengartner 2000), a myriad of biochemical reactions will have taken place, each of which is required for the development, survival an ...
Acyl Carrier Protein (ACP) lmport into Chloroplasts Does not
Acyl Carrier Protein (ACP) lmport into Chloroplasts Does not

... peptide. Substitution of serine 38 by alanine, eliminating the phosphopantetheine prosthetic group attachment site of ACP, produced a precursor mutant that gave rise to only the 14-kD peptide during import, showing that the modified form depends on the presence of serine 38. Furthermore, these resul ...
Laroche: Mouse Colouration
Laroche: Mouse Colouration

... mRNA Strand (identify 5’,3’): 5’–CUU GUC CAC CAA GGU–3’ Amino Acid Strand (identify N,C): N-Leu-Val-His-Gln-Gly- C ...
Hydroxy carboxylic acids
Hydroxy carboxylic acids

... Naturally occurring tartaric acid is chiral, meaning that it has molecules that are non-superimposable on their mirror-images. It is a useful raw material in organic chemistry for the synthesis of other chiral molecules. The naturally occurring form of the acid is L-(+)-tartaric acid or dextrotartar ...
handedness - UNIT NAME
handedness - UNIT NAME

... of death in general was lower in left-handers and mixed-handers than in right-handers of either sex. Halpern and Coren (1991) stated that it is likely that the correlates of sinistrality, not sinistrality itself, are responsible for the increased risk; left-handedness may indicate covert neuropathol ...
Couchioplanes caeruleus - International Journal of Systematic and
Couchioplanes caeruleus - International Journal of Systematic and

... of these strains fragmented during the growth cycle and produced motile spores arranged in chains within the mycelia. Sporangia were not observed. The strains contained menaquinone 9(H4), had guanine-plus-cytosine contents of 69.9 to 72.1 mol%, and had D-glutamic acid, D- and L-serine, glycine, L-al ...
application of recombinant smr-domain containing protein of
application of recombinant smr-domain containing protein of

... angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with pooled positive sera from proven angiostrongyliasis patients. The clone contained an open reading frame of 2,136 bp encoding a 80.5 kDa protein with a predicted isoelectric point of 5.8. The deduced amino aci ...
Biochemical but not clinical vitamin A deficiency results from
Biochemical but not clinical vitamin A deficiency results from

... ranges (Table 3). Because zinc deficiency is frequently associated with low plasma retinol concentrations (21), we measured plasma zinc concentrations. As shown in Table 3, plasma zinc concentrations were normal in both siblings and their mother. TTR gene analysis A missense mutation of the TTR gene ...
Citric acid cycle
Citric acid cycle

... The equilibrium of this reaction lies far to the left under standard conditions. In cells, the concentration of OAA is extremely low (<10–6 M), pulling the malate dehydrogenase reaction towards the formation of OAA. ...
Accurate Prediction of Contact Numbers for Multi
Accurate Prediction of Contact Numbers for Multi

... of residues in the protein for computing the oligomeric contact number. All nonprotein molecules were removed before computing the contact numbers. Nonprotein molecules such as coenzymes, ligands, and internal waters play important roles in the function of membrane proteins. However, the biochemical ...
New lysosomal acid lipase gene mutants explain the phenotype of
New lysosomal acid lipase gene mutants explain the phenotype of

... site of the corresponding intron) that results in exon skipping and the subsequent loss of 24 amino acids from the protein is the most frequent defect observed in patients with CESD (4, 7–9). Skipping of the same exon has also been observed in a Wolman disease patient. However, in this case, the G t ...
Evolution of Phenotypic Robustness
Evolution of Phenotypic Robustness

... (Wagner et al., 1997). Phenomena of this kind have been observed in computational models of RNA secondary structure and have been called plasto-genetic congruence (Ancel and Fontana, 2000). In order to account for congruence effects, we may further refine our definition by restricting the reference ...
¹⁵N Heteronuclear Single Quantum Coherence
¹⁵N Heteronuclear Single Quantum Coherence

... group binds with alternate orientations of 180° along the α,γ-meso axis (see Figure 2). Heme re-orientation will then progress until the equilibrium ratio of 9:1 is reached. NMR spectroscopy has been effective at monitoring this distribution of conformational isomers. Paramagnetism causes resonances ...
CFTR mutation classifications toward genotype
CFTR mutation classifications toward genotype

... infectious symptoms. The identification of the CFTR gene in 1989 gave hopes of rapidly finding a cure for the disease, for which over 1,950 mutations have been identified. Since 2012, recent approaches have enabled the identification of small molecules targeting either the CFTR protein directly or i ...
Structural Analysis of Type III Collagen Using Two Dimensional
Structural Analysis of Type III Collagen Using Two Dimensional

... including being a major component of blood vessels, internal organs, and skin. A collagen molecule comprises of over a thousand amino acids, which are all arranged in three left-handed peptide chains folded together into a characteristic right-handed triple helix. Type III collagen is known to play ...
The Drosophila pipsqueak gene encodes a nuclear BTB
The Drosophila pipsqueak gene encodes a nuclear BTB

... permitted unambiguous identification of the position of each intron relative to the cDNA (see Fig. 4A). We identified introns between the following pairs of nucleotides in the psq-1 cDNA (numbering as in Fig. 4A): (614, 615), (883, 884), (1048,1049), (2014, 2015), (2153, 2154), (2693, 2694), (3332, ...
chapter2 review of literature
chapter2 review of literature

... circumvent the short comings associated with the technology. One way to achieve this is by domesticating wild plants that accumulate oil with desirable characteristics. However, the long time scale (of over 20 years) needed to adapt them to cultivation and the requirement for remodelling of agricult ...
Trophic ecology of a green turtle breeding population
Trophic ecology of a green turtle breeding population

... diet and habitat differences among and/or within populations (Layman et al. 2007a, Martínez del Rio et al. 2009, Navarro et al. 2011). An isotopic niche is a proxy for an ecological niche and is influenced by what individuals consume (bionomic factors), as well as where they live (scenopoetic factor ...
UNIVERSITAT DE BARCELONA Divisió de Ciències Experimentals i Matemàtiques Facultat de Biologia
UNIVERSITAT DE BARCELONA Divisió de Ciències Experimentals i Matemàtiques Facultat de Biologia

... accrue more protein —in addition to much more fat— than control reference diet-fed animals (Esteve et al., 1992), and it has been generally assumed that this is may be due in part to the higher biological value of the protein of some components of the cafeteria diets. This study has been developed a ...
Modeling RNA Molecules
Modeling RNA Molecules

... and stress responses, and disease), really need to know about the 3D structures of the RNA molecules they study, and in what form do they need it? In this context, how deep do we need to go into atomic details to gain useful insights? How can knowledge of RNA 3D structure be applied to infer RNA fun ...
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Genetic code



The genetic code is the set of rules by which information encoded within genetic material (DNA or mRNA sequences) is translated into proteins by living cells. Biological decoding is accomplished by the ribosome, which links amino acids in an order specified by mRNA, using transfer RNA (tRNA) molecules to carry amino acids and to read the mRNA three nucleotides at a time. The genetic code is highly similar among all organisms and can be expressed in a simple table with 64 entries.The code defines how sequences of these nucleotide triplets, called codons, specify which amino acid will be added next during protein synthesis. With some exceptions, a three-nucleotide codon in a nucleic acid sequence specifies a single amino acid. Because the vast majority of genes are encoded with exactly the same code (see the RNA codon table), this particular code is often referred to as the canonical or standard genetic code, or simply the genetic code, though in fact some variant codes have evolved. For example, protein synthesis in human mitochondria relies on a genetic code that differs from the standard genetic code.While the genetic code determines the protein sequence for a given coding region, other genomic regions can influence when and where these proteins are produced.
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