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Immunology
Immunology

... (1) They are produced by the body in response to the presence of foreign substances. (2) They may be produced in response to an antigen. (3) They are nonspecific, acting against any foreign substance in the body. (4) They may be produced by white blood cells. 7. A part of the Hepatitis B virus is sy ...
Immunology Notes
Immunology Notes

... common cold, while another produces antibody that zeros in on a bacterium that causes pneumonia. When a B cell encounters its triggering antigen(along with collaborating T cells and accessory cells), it gives rise to many large plasma cells. Every plasma cell is essentially a factory for producing a ...
Fighting Infectious Disease
Fighting Infectious Disease

... Vaccinations stimulate immune system with antigens  Immune system responds by producing memory B cells and memory T Cells  Quicken and strengthen response ...
Non-specific host defenses
Non-specific host defenses

... – Antigenic site of a molecule (Eg. Exotoxin) ...
Biotechnology and Genetic Engineering-PBIO 450
Biotechnology and Genetic Engineering-PBIO 450

... •The amino acid sequence in the tips of the "Y" varies greatly among different antibodies. This variable region, composed of 110-130 amino acids, give the antibody its specificity for binding antigen. The variable region includes the ends of the light and heavy chains. Treating the antibody with a p ...
Cell Week6
Cell Week6

... 4. What name is given to the voltage across a muscle cell sarcolemma? 5. Inside a muscle cell there are long structures running the length of the cell. Name them. 6. Which filamentous structures do you find in a sarcomere? Explain what happens in a sarcomere when a muscle contracts 7. What event ini ...
ImprovIng Immune response In newborn Calves
ImprovIng Immune response In newborn Calves

... “Characterization of Fcy (gamma) receptor II (CD32) on B lymphocytes and its role in modulation of antibody responses in neonates” ...
The Adaptive Immune Response B
The Adaptive Immune Response B

... pathway, and complement products promote phagocytosis and destruction of microbes. The production of most opsonizing and complement-fixing IgG antibodies is stimulated by TH1 helper cells, which respond to many bacteria and viruses; thus, the protective response to most bacteria and viruses is drive ...
elisa - WordPress.com
elisa - WordPress.com

... solution that when oxidized by HRP turns yellow. A substrate is a compound or substance that undergoes change. – Substrates bind to active sites on the surface of enzymes and are converted or changed. In ELISA the specific substrate used changes color. – Substrate Solution: • chromogen A and • chrom ...
Immunology (B)
Immunology (B)

... A. are joined to heavy chains by peptide bonds B. can be present as both k and l chains as part of a single Ig molecule C. are not found in every major immunoglobulin class D. all have the same amino acid composition E. are present in the Fab fragment of Ig 8. A B cell can express on its cell surfac ...
BIOTECHNOLOGY Biology W4034/3034 Final Exam Dec
BIOTECHNOLOGY Biology W4034/3034 Final Exam Dec

... it to an E. coli promoter for production of the domain in E. coli. Attach it to some bead material. UseT7 RNA polymerase to synthesize 1014 molecules of random 20-mers from a synthetic DNA templates consisting of a T7 promoter followed by an arbitrary 20-nt PCR primer sequence, 20 random nucleotides ...
Introduction to flow cytometry
Introduction to flow cytometry

... Flow cytometry is now a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell populations, assessing the purity of isolated subpopulations and analyzing cell size and volume. It allows sim ...
1. The Cell-Mediated Immune Response
1. The Cell-Mediated Immune Response

... The initial exposure of a B cell to its specific antigen results in its activation as follows: 1) dendritic cell, macrophage or B cell ingests extracellular antigen by phagocytosis 2) peptides fr. antigen presented on MHC class II 3) specific TH cells activated to become TH2 cells 4) TH2 cells in tu ...
The Rh System
The Rh System

... Both parents have one haplotype that is a total Rh deletion, for example Dce/-- Each parent passes the deletion on to the ...
The Immune System
The Immune System

... • Vasodilation of the microcirculation (small blood vessels) resulting in increased blood flow. • An increase in vascular permeability to protein. • Filtration of fluid into the tissue which leads to swelling. • Neutrophils (a type of white blood cell) and later monocytes (another type of white bloo ...
Immunity (Ag).
Immunity (Ag).

... (serum) to test for presence of antibody. • Place target sample on a support. • Add serum that has antibody against antigen being tested for. Antibody, if present, binds the antigen. • Add a second antibody that was separately developed to react with the antibody/antigen complex in step B and binds ...
The Immune System
The Immune System

... 2. T cell response, an active, cell-mediated defense that involves the destruction of pathogens by cytotoxic T cells ...
Effect of Sugar Intake on Phagocytosis
Effect of Sugar Intake on Phagocytosis

...  Therefore, “I think we got it all” can best be translated, “I can’t see or feel anymore cancer, so let’s hope the immune system will destroy that which is probably still there.” ...
Immune Systm.graffle
Immune Systm.graffle

... The ability of the body to defend itself against pathogens or poisons depends on the immune system. The T helper cells have the ability to recognize antigens (foreign substance). Once this is done, other cells (B cells) must make special molecules out of protein that attach to the antigen. These spe ...
cancer treatment that uses monoclonal antibodies (download only)
cancer treatment that uses monoclonal antibodies (download only)

... Worldwide, 1.6 million new cases of breast cancer are diagnosed each year, leading to over half a million deaths. 40 years ago in the UK, the five-year survival rate following diagnosis of breast cancer was 50%. Today that figure is 80% thanks to advances in treatment and screening. One of these adv ...
Specific Host Defense IMMUNOLOGY
Specific Host Defense IMMUNOLOGY

... • L chain located at the top of the Y • The tops of both H & L are the Antigenbinding sites (Fab) - Bivalent in which a.a. sequences are variable (VH, VL) • The sequences of the rest: Constant (Fc) ...
Immunogens in the generation of Triple A Polyclonals and PrecisA
Immunogens in the generation of Triple A Polyclonals and PrecisA

... Atlas Antibodies and the Human Protein Atlas project (HPA)3,4,5,6 use these PrEST Antigens for immunization. The resulting polyclonal antibodies are affinity purified against the corresponding PrEST Antigens in a three-step procedure to remove the tag-specific antibodies and to catch the PrEST-speci ...
Basic Immunology
Basic Immunology

... ANTIBODY GENERATOR: foreign substance induces antibody production ...
serving up cancer cells - Ludwig Institute for Cancer Research
serving up cancer cells - Ludwig Institute for Cancer Research

... immune responses of patients in the upcoming clinical trial. They will investigate whether the patients’ T cells are activated by anti-CD47 antibodies, as they are in mice. The scientists are also planning animal studies to examine whether anti-CD47 antibody treatment can be powered up by drugs that ...
Lymphatic System Notes- Chapter 12
Lymphatic System Notes- Chapter 12

... *Most become _______________ that secrete _____________________ and last _____ * Some become __________________ and cause a _____________ response the 2nd time -T cells become immunocompetent in ________________________ *Cytotoxic T cells- specialized in _______________________________ *____________ ...
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Monoclonal antibody



Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are made by identical immune cells that are all clones of a unique parent cell, in contrast to polyclonal antibodies which are made from several different immune cells. Monoclonal antibodies have monovalent affinity, in that they bind to the same epitope.Given almost any substance, it is possible to produce monoclonal antibodies that specifically bind to that substance; they can then serve to detect or purify that substance. This has become an important tool in biochemistry, molecular biology and medicine. When used as medications, the non-proprietary drug name ends in -mab (see ""Nomenclature of monoclonal antibodies""), and many immunotherapy specialists use the word mab anacronymically.
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