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Basic Chemistry and Major Biomolecules
Basic Chemistry and Major Biomolecules

... It is more energetically favorable for nonpolar compounds to aggregate together in water than stay apart. Place two drops of vegetable oil in a bowl of water, and with some time they will collide and become one. The warmer the water the faster this happens. Why? Cell membranes form from lipids due t ...
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... visualized using the Jalview software [1]. Intuitively, one would expect to see greater sequence homology in regions encoding orthologous SH3 domains and higher sequence divergence in adjacent, nonconserved regions. Aligning Bem1p orthologues from several fungal species revealed a highly conserved 5 ...
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... construct used for targeting CSN2 locus in bovine mammary epithelial cells with ZFNs. (D) human lysozyme and EGFP fusion protein was expressed in the cells. The bovine mammary epithelial cells were transfected by pEGFP-C-hLYZ. (E) The expressed human lysozyme and EGFP fusion protein was secreted in ...
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Cell-penetrating peptide



Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.
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